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Hao Y.,Shangqiu Normal University | Chen W.,Central South University | Wang L.,Central South University | Zhu X.,Shangqiu Normal University | And 7 more authors.
Talanta | Year: 2015

A novel fluorescent probe C-GGH for recognition of Cu2+ and S2- was designed and synthesized by incorporation a natural tripeptide GGH (Gly-Gly-His) moiety, which acts as a recognition unit for Cu2+, to a coumarin fluorophore. C-GGH displayed ON-OFF type fluorescence response towards Cu2+ with high selectivity in 100% aqueous solution. Furthermore, the in situ generated non-fluorescent C-GGH-Cu2+ complex can serve as an effective OFF-ON type fluorescent probe for selective sensing sulfide anion. Due to the strong binding affinity of S2- to Cu2+, sulfide anion can extract Cu2+ from C-GGH-Cu2+ complex, resulting the release of C-GGH and the revival of fluorescent emission of the system. Techniques of emission spectrometry, NMR, mass spectrometry, and HPLC were used to confirm the displacement mechanism of C-GGH-Cu2+ for sensing sulfide anion. The probe C-GGH-Cu2+ allowed detection of S2- in aqueous buffer solution with a LOD (limit of detection) of 0.44 μM. By taking advantages of the natural tripeptide GGH, the sensor exhibits excellent biocompatibility and water solubility. Moreover, the cell imaging study demonstrated that the probe can be exploited to detect sulfur anion in biological systems. © 2015 Published by Elsevier B.V.


Li Z.-W.,Changsha University | Li J.-S.,Changsha University | Kang S.-Y.,Hunan Institute of Food Quality Supervision Inspection and Research | Liu W.-D.,Hunan Institute of Engineering | And 2 more authors.
Jiegou Huaxue | Year: 2013

The title compound (2-iodo-5-nitrophenyl)-[1-(1-methylazepan-3-yl)-1H- indol-3-yl]methanone (C22H22IN3O3, Mr=503.07 ) was synthesized by the reaction of 3-(2-iodo-5-nitrobenzoyl)indole with 2-chloromethyl-1-methylpiperidine. Its chemical structure was determined by 1H NMR, 13C NMR, DEPT, COSY, HMQC, HMBC, HRMS and X-ray single-crystal diffraction. The crystal belongs to the triclinic system, space group P1 with a=9.153(4), b=10.409(3), c=11.882(4) , α=71.84(3), =78.67(3), =75.49(3), V=1032.7(6) 3, Z=2, Dc=1.619 g/cm3, μ=1.579 mm-1, F(000)=504, R=0.0270 and wR=0.0498 for 3634 observed reflections with I > 2(I). X-ray analysis shows that the indole ring forms a dihedral angle of 84.57(12) with the iodobenzene ring and the azepane ring adopts a twisted chair conformation. Weak C-HO hydrogen bonding contributes to the stability and packing of the structure.


Gu Y.-Y.,Central South University | Xu W.-Y.,Hunan Institute of Food Quality Supervision Inspection and Research | Zhou T.-Y.,Changsha Bureau of Commerce
Chinese Rare Earths | Year: 2014

Pechini method was taken to synthesize perovskite-like mixed oxides La2-xSrxCuO4 (x =0 ~0.4); XRD, TG, SEM, BET and H2-TPR were used to characterize the samples obtained. The effects of the amount of citric acid, calcination temperature and the substitution amount of Sr on the phase formation, specific area and catalytic capability have been investigated. The experiment shows that when citric acid-to-metal nitrates molar ratio and calcination temperature reaches 4 and 800°C respectively, the sample obtained is single phase with good particle distribution and satisfying crystallization. XRD shows that when x > 0, all the samples obtained are single-phase perovskite-like mixed oxides. With the increase of x, the specific surface areas of the samples increase and the catalytic performance of samples at low temperature has been improved.


Chen C.-D.,Pingdingshan University | La M.,Pingdingshan University | Zhou B.-B.,Hunan Institute of Food Quality Supervision Inspection and Research | Zhou B.-B.,Central South University
International Journal of Electrochemical Science | Year: 2014

Electrochemical genosensors have held great promise as devices suitable for point-of-care diagnostics and multiplexed platforms for fast, simple and inexpensive nucleic acid analysis. MicroRNA (miRNA), regulating gene expression by translational repression or degradation of messenger RNA, is believed to be important for cancer diagnosis and prognosis serving as a reliable molecular biomarker. In recent years, there have been many attempts for miRNA detection using electrochemical genesensors. In this work, we first addressed the progress in designing of miRNA detection strategies based on the difference in the structure of RNA and DNA. This work should be valuable for the design of new types of miRNA genesensors. © 2014 The Authors.


PubMed | Central South University, Hunan Institute of Food Quality Supervision Inspection and Research and Shangqiu Normal University
Type: | Journal: Talanta | Year: 2015

A novel fluorescent probe C-GGH for recognition of Cu(2+) and S(2-) was designed and synthesized by incorporation a natural tripeptide GGH (Gly-Gly-His) moiety, which acts as a recognition unit for Cu(2+), to a coumarin fluorophore. C-GGH displayed ON-OFF type fluorescence response towards Cu(2+) with high selectivity in 100% aqueous solution. Furthermore, the in situ generated non-fluorescent C-GGH-Cu(2+) complex can serve as an effective OFF-ON type fluorescent probe for selective sensing sulfide anion. Due to the strongbindingaffinity of S(2-) to Cu(2+), sulfide anion can extract Cu(2+) from C-GGH-Cu(2+) complex, resulting the release of C-GGH and the revival of fluorescent emission of the system. Techniques of emission spectrometry, NMR, mass spectrometry, and HPLC were used to confirm the displacement mechanism of C-GGH-Cu(2+) for sensing sulfide anion. The probe C-GGH-Cu(2+) allowed detection of S(2-) in aqueous buffer solution with a LOD (limit of detection) of 0.44M. By taking advantages of the natural tripeptide GGH, the sensor exhibits excellent biocompatibility and water solubility. Moreover, the cell imaging study demonstrated that the probe can be exploited to detect sulfur anion in biological systems.


Li S.,Central South University | Wang L.,Central South University | Hao Y.,Central South University | Zhang L.,Central South University | And 4 more authors.
RSC Advances | Year: 2014

A peptide nanosphere-based colorimetric aptasensor for the ultrasensitive detection of adenosine triphosphate (ATP) has been fabricated. By conjugating gold nanoparticles (AuNPs) onto peptide nanospheres (PNS), the PNS/AuNPs nanocomposite was obtained and used as a carrier for single-strand ATP aptamer (S1) and signaling DNAzyme sequence. In the presence of ATP, the nucleic acids decorated PNS/AuNPs could immobilize to a 96-well plate which was modified with another single-strand ATP aptamer (S2) DNA. Then, upon addition of K+ and hemin, the designed signaling DNAzyme sequence on PNS/AuNPs could form the hemin-G-quadruplex DNAzyme and catalyze the conversion of TMB to coloured TMB2+. The colorimetric aptasensor adopting PNS/AuNPs nanocomposites as signal enhancers possesses much higher sensitivity towards ATP compared with the conventional AuNPs-based aptasensors. The PNS/AuNPs-based sensor is available for sensitively detecting ATP in a concentration range of 0.01-1 nM with a detection limit of 1.35 pM. Moreover, the constructed aptasensor displayed excellent selectivity for ATP over other analogues such as GTP, CTP and UTP. The proposed strategy for the construction of an aptasensor based on nanocomposites has great potential to become a universal technique for developing various aptasensors by using different aptamers. © 2014 the Partner Organisations.


Hao Y.,Central South University | Chen W.,Central South University | Wang L.,Central South University | Zhou B.,Central South University | And 4 more authors.
Analytical Methods | Year: 2014

A naphthalimide-based azo dye (1) used as a colorimetric and ratiometric probe for the detection of CN- was synthesized by incorporating a salicylaldehyde moiety, which acts as a recognition unit, to the naphthalimido diazonium salt. Upon reacting with CN-, probe 1 displays dramatic colour changes rapidly from yellowish to red, corresponding to the absorption band shift from 408 nm to 497 nm. The colorimetric probe allows ratiometric detection of CN- with a linear range of 2-45 μM and a LOD (limit of detection) of 0.4 μM. In the optimized H2O/DMSO (7/3, v/v) mixed solvent, probe 1 displays excellent selectivity for CN- over other anions. In addition, 1 was successfully employed to prepare the filter paper-based test strips which can be used to monitor CN- conveniently, and the discernible concentration of CN- can be as low as 20 μM. © 2014 the Partner Organisations.


Zhou B.,Central South University | Zhou B.,Hunan Institute of Food Quality Supervision Inspection and Research | Sun Z.,Central South University | Li D.,Central South University | And 3 more authors.
Nanoscale | Year: 2013

An aniline-GGAAKLVFF peptide (AFP) was prepared by solid-phase synthesis. The peptide can readily self-assemble into fibrils. Platinum nanoparticles (Pt NPs) were directly immobilized on the surface of the AFP fibrils via electrostatic interaction. Compared to other currently available techniques for the fabrication of metal-peptide fibrils, the noncovalent functionalization strategy is able to deposit nanoparticles on peptide fibrils with different morphologies and high metal loading, which is important for applications in catalysis, electronic materials and other corresponding fields. The Pt-AFP fibrils were employed to modify the electrode, which exhibits high electrocatalytic activities towards oxygen reduction. Thus, the Pt-AFP fibrils hold great potential for polymer electrolyte fuel cells and other electrochemical applications. © The Royal Society of Chemistry 2013.


Hao Y.,Central South University | Zhou B.,Central South University | Zhou B.,Hunan Institute of Food Quality Supervision Inspection and Research | Wang F.,Hunan Institute of Food Quality Supervision Inspection and Research | And 3 more authors.
Biosensors and Bioelectronics | Year: 2014

A novel electrochemical active polyaniline (PANI) nanowire was fabricated and utilized for the construction of a highly sensitive and selective electrochemical sensor for hepatitis B virus gene. The uniform PANI nanowire was prepared by the enzymatic polymerization of aniline monomers on the amyloid-like nanofiber (AP nanowire), which was self-assembled from an aniline-attached nonapeptide, aniline-GGAAKLVFF (AP). The prepared PANI nanowires were characterized by electron microscopy, UV-vis absorption spectra, and cyclic voltammetry (CV). These ultra-thin nanowires displayed high electrochemical activity. Then the nucleic acid biosensor was constructed by modifying a glass carbon electrode with AP nanowires which were functionalized by a designed hair-pin loop DNA. Upon the presence of target nucleic acid and horseradish peroxidase (HRP) labeled oligonucleotide, the HRP will catalyze the polymerization of aniline monomers conjugated in AP nanowires, leading to the formation of PANI nanowires which can bring about a dramatical increase in the current response of the biosensor. The dynamic range of the sensor for hepatitis B virus gene is 2.0-800.0. fM with a low detection limit of 1.0. fM (3. σ, n=10). The biosensor also displayed highly selectivity and stability. All these excellent performances of the developed biosensor indicate that this platform can be easily extended to the detection of other nucleic acids. © 2013 Elsevier B.V.


Hao Y.,Central South University | Xiong D.,Central South University | Wang L.,Central South University | Chen W.,Central South University | And 3 more authors.
Talanta | Year: 2013

A novel reversible colorimetric sensor, which based on a competitive ligation of Hg2+ by thiols, cysteine (Cys) or glutathione (GSH), and thiocyanate (SCN) on the N3 dye (bis(4,4′-dicarboxy-2,2′-bipyridine) dithiocyanato ruthenium (II)), was developed for the detection of biothiols. First, Hg2+ ions coordinate to the sulfur atom of the dyes' SCN groups, and this interaction induces a change in color from red to yellow, owing to the formation of a complex of Hg2+-N3. Then, in the presence of biothiols, the red color of N3 is recovered concomitantly with the dissociation of the Hg2+-N3 complex, due to the extraction of Hg2+ by biothiols. Thus the corresponding color variation in the process of the dissociation of the Hg2+-N3 complex can be employed for the quantitative detection of thiols using UV-vis spectroscopy. In particular, the transformation can be readily viewed with the naked eye. A good linear relationship between the change in absorbance (ΔAbs) of Hg2+-N3 at 461 nm and the thiol concentration was obtained in the range of 0.5-25 μM, and the detection limits are then calculated to be 57 and 52 nM for Cys and GSH, respectively. The proposed colorimetric assay displays a high selectivity for Cys over various other amino acids and GSSG (oxidized glutathione). © 2013 Elsevier B.V.

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