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Chen C.-D.,Pingdingshan University | La M.,Pingdingshan University | Zhou B.-B.,Hunan Institute of Food Quality Supervision Inspection and Research | Zhou B.-B.,Central South University
International Journal of Electrochemical Science | Year: 2014

Electrochemical genosensors have held great promise as devices suitable for point-of-care diagnostics and multiplexed platforms for fast, simple and inexpensive nucleic acid analysis. MicroRNA (miRNA), regulating gene expression by translational repression or degradation of messenger RNA, is believed to be important for cancer diagnosis and prognosis serving as a reliable molecular biomarker. In recent years, there have been many attempts for miRNA detection using electrochemical genesensors. In this work, we first addressed the progress in designing of miRNA detection strategies based on the difference in the structure of RNA and DNA. This work should be valuable for the design of new types of miRNA genesensors. © 2014 The Authors.

Gu Y.-Y.,Central South University | Xu W.-Y.,Hunan Institute of Food Quality Supervision Inspection and Research | Zhou T.-Y.,Changsha Bureau of Commerce
Chinese Rare Earths | Year: 2014

Pechini method was taken to synthesize perovskite-like mixed oxides La2-xSrxCuO4 (x =0 ~0.4); XRD, TG, SEM, BET and H2-TPR were used to characterize the samples obtained. The effects of the amount of citric acid, calcination temperature and the substitution amount of Sr on the phase formation, specific area and catalytic capability have been investigated. The experiment shows that when citric acid-to-metal nitrates molar ratio and calcination temperature reaches 4 and 800°C respectively, the sample obtained is single phase with good particle distribution and satisfying crystallization. XRD shows that when x > 0, all the samples obtained are single-phase perovskite-like mixed oxides. With the increase of x, the specific surface areas of the samples increase and the catalytic performance of samples at low temperature has been improved.

Li S.,Central South University | Wang L.,Central South University | Hao Y.,Central South University | Zhang L.,Central South University | And 4 more authors.
RSC Advances | Year: 2014

A peptide nanosphere-based colorimetric aptasensor for the ultrasensitive detection of adenosine triphosphate (ATP) has been fabricated. By conjugating gold nanoparticles (AuNPs) onto peptide nanospheres (PNS), the PNS/AuNPs nanocomposite was obtained and used as a carrier for single-strand ATP aptamer (S1) and signaling DNAzyme sequence. In the presence of ATP, the nucleic acids decorated PNS/AuNPs could immobilize to a 96-well plate which was modified with another single-strand ATP aptamer (S2) DNA. Then, upon addition of K+ and hemin, the designed signaling DNAzyme sequence on PNS/AuNPs could form the hemin-G-quadruplex DNAzyme and catalyze the conversion of TMB to coloured TMB2+. The colorimetric aptasensor adopting PNS/AuNPs nanocomposites as signal enhancers possesses much higher sensitivity towards ATP compared with the conventional AuNPs-based aptasensors. The PNS/AuNPs-based sensor is available for sensitively detecting ATP in a concentration range of 0.01-1 nM with a detection limit of 1.35 pM. Moreover, the constructed aptasensor displayed excellent selectivity for ATP over other analogues such as GTP, CTP and UTP. The proposed strategy for the construction of an aptasensor based on nanocomposites has great potential to become a universal technique for developing various aptasensors by using different aptamers. © 2014 the Partner Organisations.

Zhao L.-G.,Lanzhou University | Chen T.-Q.,Lanzhou University | Chen T.-Q.,Hunan Institute of Food Quality Supervision Inspection and Research | Feng D.-M.,Peoples Hospital of Gansu Province | And 4 more authors.
Journal of Asian Natural Products Research | Year: 2014

A water-soluble polysaccharide (HPS3aS) with a molecular mass of 1.22 × 104 Da was isolated from Hedysarum polybotrys using anion-exchange and gel-permeation chromatography. HPS3aS exhibits a globular-shaped conformation in 0.1M NaNO3 by size exclusion chromatography with multi-angle laser light scattering (SEC-MALLS). The investigation of the structural features of this heteropolysaccharide through a combination of chemical and instrumental analyses revealed that the backbone of HPS3aS is composed of α-D-(1 → 4)-linked glucopyranose residues, which occasionally branched at O-6. The branches are composed of (1 → 4)-linked galactopyranose residues and terminated with glucopyranose residues. HPS3aS possesses good in vitro antioxidant activity, as evaluated by scavenging assays with 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, and superoxide radicals, which suggests that HPS3aS could be a potential antioxidant. © 2014 Taylor & Francis.

Hao Y.,Central South University | Chen W.,Central South University | Wang L.,Central South University | Zhou B.,Central South University | And 4 more authors.
Analytical Methods | Year: 2014

A naphthalimide-based azo dye (1) used as a colorimetric and ratiometric probe for the detection of CN- was synthesized by incorporating a salicylaldehyde moiety, which acts as a recognition unit, to the naphthalimido diazonium salt. Upon reacting with CN-, probe 1 displays dramatic colour changes rapidly from yellowish to red, corresponding to the absorption band shift from 408 nm to 497 nm. The colorimetric probe allows ratiometric detection of CN- with a linear range of 2-45 μM and a LOD (limit of detection) of 0.4 μM. In the optimized H2O/DMSO (7/3, v/v) mixed solvent, probe 1 displays excellent selectivity for CN- over other anions. In addition, 1 was successfully employed to prepare the filter paper-based test strips which can be used to monitor CN- conveniently, and the discernible concentration of CN- can be as low as 20 μM. © 2014 the Partner Organisations.

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