Hunan Institute of Animal and Veterinary Science

Changsha, China

Hunan Institute of Animal and Veterinary Science

Changsha, China

Time filter

Source Type

Xiao D.,Hunan Agricultural University | Xiao D.,CAS Institute of Subtropical Agriculture | Tang Z.,Southwest University | Yin Y.,CAS Institute of Subtropical Agriculture | And 4 more authors.
International Immunopharmacology | Year: 2013

This study was conducted to investigate how chitosan (COS) affects intestinal mucosal barrier function and to further explain mechanisms of COS on growth performance. Thirty piglets, weaned at 21 days of age, were challenged with enterotoxigenic Escherichia coli during preliminary trial period. Three groups of Piglets in individual pens were fed a corn-soybean meal diet containing no addition, 50 mg/kg chlortetracycline, or 300 mg/kg COS for 21 days. Jejunal morphology and histology were analyzed under light microscope. The concentrations of occludin proteins were determined by western blot. Immunohistochemistry assays were used to determine secretory immunoglobulin (sIgA) level. Real-time PCR was used to detect Toll-like receptor 4 (TLR4) and Claudin-1 in jejunal mucosa. Feeding COS or chlortetracycline reduced (P < 0.05) feed conversion ratio. Villus length, villus length/crypt depth, and goblet cells, were increased (P < 0.05), but villus width and crypt depth were decreased (P < 0.05) in both COS and chlortetracycline groups. Intraepithelial lymphocytes were higher (P < 0.05) in the COS group than both chlortetracycline and control groups. Occludin protein expression was increased (P < 0.01) in the COS group, but was decreased (P < 0.05) in the chlortetracycline group. Expression of sIgA protein was higher (P < 0.05) in the COS group than both control and chlortetracycline groups, however TLR4 mRNA expression was decreased (P < 0.05) in both COS and chlortetracycline groups. There was no difference in expression of claudin-1 among the three groups. In conclusion, chitosan and the antibiotic have similar effects in promoting piglet growth and reducing intestinal inflammation, but different effects on intestinal mucosal barrier function. This indicates that chitosan can replace chlortetracycline as a feed additive for piglets. © 2013 Published by Elsevier B.V.


Chen C.,Huazhong Agricultural University | Chen C.,Hunan Institute of Animal and Veterinary Science | Peng Y.,Huazhong Agricultural University | Peng Y.,Hunan Institute of Animal and Veterinary Science | And 2 more authors.
Journal of Molecular Endocrinology | Year: 2014

MicroRNAs are endogenous, conserved, and non-coding small RNAs that function as post-transcriptional regulators of fat development and adipogenesis. Adipogenic marker genes, such as CCAAT/enhancer binding protein α (Cebpa), peroxisome proliferator-activated receptor γ (Pparg), adipocyte fatty acid binding protein (Ap2), and fatty acid synthase (Fas), are regarded as the essential transcriptional regulators of preadipocyte differentiation and lipid storage in mature adipocytes. Canonical Wnt/β-catenin signaling is recognized as a negative molecular switch during adipogenesis. In the present work we found that miR-135a-5p is markedly downregulated during the process of 3T3-L1 preadipocyte differentiation. Overexpression of miR-135a-5p impairs the expressions of adipogenic marker genes as well as lipid droplet accumulation and triglyceride content, indicating the importance of miR-135a-5p for adipogenic differentiation and adipogenesis. Further studies show that miR-135a-5p directly targets adenomatous polyposis coli (Apc), contributes to the translocation of β-catenin from cytoplasm to nucleus, and then activates the expressions of cyclin D1 (Ccnd1) and Cmyc, indicating the induction of canonical Wnt/β-catenin signaling. In addition, inhibition of APC with siRNA exhibits the same effects as overexpression of miR-135a-5p. Our findings demonstrate that miR-135a-5p suppresses 3T3-L1 preadipocyte differentiation and adipogenesis through the activation of canonical Wnt/β-catenin signaling by directly targeting Apc. Taken together, these results offer profound insights into the adipogenesis mechanism and the development of adipose tissue. © 2014 Society for Endocrinology.


Trefil P.,Research Institute of Biopharmacy and Veterinary Drugs | Bakst M.R.,U.S. Department of Agriculture | Yan H.,Hunan Institute of Animal and Veterinary Science | Hejnar J.,Academy of Sciences of the Czech Republic | And 2 more authors.
Theriogenology | Year: 2010

Transplantation of male germ line cells into sterilized recipients has been used in mammals for conventional breeding as well as for transgenesis. We have previously adapted this approach for the domestic chicken and we present now an improvement of the germ cell transplantation technique by using an enriched subpopulation of c-Kit-positive spermatogonia as donor cells. Dispersed c-Kit positive testicular cells from 16 to 17 week-old pubertal donors were transplanted by injection directly into the testes of recipient males sterilized by repeated gamma irradiation. We describe the repopulation of the recipient's testes with c-Kit positive donor testicular cells, which resulted in the production of functional heterologous spermatozoa.Using manual semen collection, the first sperm production in the recipient males was observed about nine weeks after the transplantation. The full reproduction cycle was accomplished by artificial insemination of hens and hatching of chickens. © 2010 Elsevier Inc.


Chen C.,Huazhong Agricultural University | Chen C.,Hunan Institute of Animal and Veterinary Science | Xiang H.,Huazhong Agricultural University | Peng Y.-L.,Hunan Institute of Animal and Veterinary Science | And 2 more authors.
Cellular Signalling | Year: 2014

Differentiation of preadipocytes into adipocytes and the formation of the subsequent adipose tissue are critical for mammalian growth and development. The molecular mechanism relating to preadipocyte differentiation and adipogenesis from the perspective of miRNAs is not yet completely understood. Here we investigated whether miR-183 functioned in the differentiation process. Both gain-of-function and loss-of-function assays demonstrated that miR-183 positively regulated 3T3-L1 differentiation by enhancing the expression of adipogenic marker genes such as CCAAT/enhancer binding protein α (C/EBPα), peroxisome proliferator-activated receptor γ (PPARγ), adiponectin and fatty acid synthase (FAS), as well as the triglyceride content and accumulation of lipid droplets. Meanwhile, low-density lipoprotein receptor-related protein 6 (LRP6) was known to impair the canonical Wnt/β-catenin signaling pathway and thereafter reduce c-myc and nuclear β-catenin protein. We showed that the inhibition of LRP6 by siRNA promoted 3T3-L1 adipogenic differentiation and adipogenesis. Further analysis showed that mouse miR-183 gene had its own transcription unit containing CpG islands, transcription start site (TSS), coding sequence (CDS) and polyA signal within the flanking sequences 2500. nt upstream and downstream of mouse miR-183 in genome. The core promoter of miR-183 gene was identified and transcription factor GATA3 (GATA binding protein 3) significantly inhibited the expression of mature miR-183 by binding to its core promoter in vivo, as indicated by thechromatin immunoprecipitation (ChIP) assay. These results suggest that miR-183, though negatively regulated by transcription factor GATA3, enhances 3T3-L1 preadipocyte differentiation and adipogenesis through the inhibition of the canonical Wnt/β-catenin signaling pathway by targeting LRP6. © 2014 Elsevier Inc.


Li C.,Jilin University | Sun Y.,Jilin University | Sun Y.,Jilin Agricultural University | Yi K.,Hunan Institute of Animal and Veterinary Science | And 3 more authors.
Theriogenology | Year: 2010

The objective was to confirm the presence of nerve growth factor (NGF) and its specific receptor, TrkA, in ejaculated bovine sperm, and to investigate the effects of NGF on specific aspects of bovine sperm function. Both TrkA transcripts and immunoreactivity typical of the translated protein were detected by RT-PCR and western blotting. However, only the NGF protein was detected in bovine sperm using western blotting, and there was no RT-PCR evidence for NGF transcripts in sperm. Using an immunofluorescent technique, NGF-immunoreactivity was localized to the sperm head and tail, whereas that of TrkA was detected in the acrosomal cap, nucleus, and tail regions When sperm were treated with exogenous NGF, both leptin secretion and sperm viability were increased (P < 0.05); moreover, the percentages of late apoptotic and dead sperm were increased (P < 0.05). However, NGF had no effects on insulin secretion, mitochondrial activity, intracellular calcium levels, or the acrosome reaction of sperm (P > 0.05). In conclusion, the presence of TrkA transcript, as well as NGF and TrkA immunoreactivity were confirmed in bovine sperm. Furthermore, exogenous NGF had significant effects on the secretion of leptin, cell viability, and sperm apoptosis. This study provided strong evidence that NGF/TrkA may have roles in regulation of sperm physiology and perhaps male fertility and infertility. © 2010 Elsevier Inc.


Zhang W.,Jilin University | Yi K.,Hunan Institute of Animal and Veterinary Science | Yan H.,Hunan Institute of Animal and Veterinary Science | Zhou X.,Jilin University
Animal Reproduction Science | Year: 2012

There have been intensive attempts to establish reliable in vitro production (IVP) and cryopreservation methods of embryos in pigs. Although a great deal of progress has been made, current IVP systems and cryopreservation still suffer from insufficient cytoplasmic abilities of in vitro matured oocytes, polyspermic fertilization, poor quality of in vitro produced embryos and low efficiency of embryo cryopreservation. Compared to other mammalian species, pig oocytes and embryos are characterized by large amounts of lipid content stored mainly in the form of lipid droplets in the cytoplasm. This fact has a negative influence on biotechnological applications on porcine oocytes and embryos. In this review, we will discuss recent studies about methods and techniques for modifying porcine embryo IVP system and embryo cryopreservation that produces high quality of pig blastocysts using in vitro maturation, in vitro fertilization, in vitro culture, microsurgical manipulation, addition of protein, the use of cytoskeleton stabilizing agents and various physical methods. The presented methods and techniques make it possible to modify the characteristics of oocytes and embryos and thus may become major tools in mammalian gamete and embryo agricultural or biotechnological applications in the future. © 2012 Elsevier B.V.


Zhang W.,Jilin University | Yi K.,Hunan Institute of Animal and Veterinary Science | Chen C.,Jilin University | Hou X.,Jilin University | Zhou X.,Jilin University
Animal Reproduction Science | Year: 2012

Although cryopreserved boar semen has been available since 1975, a major breakthrough in commercial application has not yet occurred due to the high susceptibility of boar spermatozoa to damage during cryopreservation and the complicated process required for deep freezing. In recent years, the application of antioxidants during the cryopreservation of boar semen has been the subject of considerable research aimed at improving the quality of post-thaw semen. Centrifugation is necessary before using cryopreservation protocols for freezing boar spermatozoa. Studies of the effect of different centrifugation regimens on boar sperm recovery, yield and cryosurvival have made significant contributions. Therefore this review elucidates results of recent applications of various antioxidants and centrifugation regimens used in efforts to improve cryopreservation of boar spermatozoa. This review is intended to enhance understanding of the roles of these antioxidants and centrifugation regimens with respect to mechanisms that increase resistance to cryodamage of boar spermatozoa. In addition, the discussion addresses the need for developing an objective evaluation of effectiveness and estimating the prospect of application of new techniques for the cryopreservation of boar semen and its use in artificial insemination. © 2012 Elsevier B.V.


PubMed | Hunan Institute of Animal and Veterinary Science and Hunan Agricultural University
Type: | Journal: BioMed research international | Year: 2016

The purpose of this study was to explore relationships between low dose dietary supplementation with chitosan (COS) and body weight, feed intake, intestinal barrier function, and permeability in mice. Twenty mice were randomly assigned to receive an unadulterated control diet (control group) or a dietary supplementation with 30mg/kg dose of chitosan (COS group) for two weeks. Whilst no significant differences were found between the conditions for body weight or food and water intake, mice in the COS group had an increased serum D-lactate content (P < 0.05) and a decreased jejunal diamine oxidase (DAO) activity (P < 0.05). Furthermore, mice in COS group displayed a reduced expression of occludin and ZO-1 (P < 0.05) and a reduced expression of occludin in the ileum (P < 0.05). The conclusion drawn from these findings showed that although 30mg/kg COS-supplemented diet had no effect on body weight or feed intake in mice, this dosage may compromise intestinal barrier function and permeability. This research will contribute to the guidance on COS supplements.


PubMed | King Saud University, Hunan Institute of Animal and Veterinary Science, CAS Institute of Subtropical Agriculture and Hunan Agricultural University
Type: | Journal: Mediators of inflammation | Year: 2016

C57BL/6 mice were tested in order to investigate the effects of dietary chitosan (COS) supplements on intestinal microflora and resistance to


PubMed | King Saud University, Hunan Institute of Animal and Veterinary Science, CAS Institute of Subtropical Agriculture and Hunan Agricultural University
Type: | Journal: Mediators of inflammation | Year: 2017

This study aims to investigate the effects of dietary chitosan (COS) on gastrointestinal pathogen resistance in mice model. For two weeks, a control group of ICR mice received a basal diet whilst the intervention group received the basal diet supplemented with 300mg/kg COS. After two weeks, the mice fed the supplemented diet had a lower body weight. Then enterotoxigenic

Loading Hunan Institute of Animal and Veterinary Science collaborators
Loading Hunan Institute of Animal and Veterinary Science collaborators