Hunan Geriatric Hospital
Hunan Geriatric Hospital
Wang Z.,Eighth Hospital of Changsha |
Wang B.,Eighth Hospital of Changsha |
Guo H.,Shouguang Hospital of Traditional Chinese Medicine |
Shi G.,Shouguang Hospital of Traditional Chinese Medicine |
Hong X.,Hunan Geriatric Hospital
Drug Design, Development and Therapy | Year: 2014
Background: Previous studies demonstrate that T-cadherin is a candidate tumor suppressor in several types of human tumors, including non-small cell lung cancer (NSCLC). Lack of protein expression of T-cadherin by hypermethylation has been found to play an important role in lung alveolar differentiation regulation and epithelial tumorigenesis. However, the correlation between T-cadherin hypermethylation and clinicopathological characteristics of NSCLC remains unclear. Here we conducted a systematic review and meta-analysis to quantitatively evaluate the effects of T-cadherin hypermethylation on the incidence of NSCLC and clinicopathological characteristics.Methods: A detailed literature search was carried out for related research publications. Analyses of pooled data were performed. Odds ratio (OR) and hazard ratio (HR) were calculated and summarized, respectively.Results: Final analysis of 1,172 NSCLC patients from 15 eligible studies was performed. T-cadherin hypermethylation was observed to be significantly higher in NSCLC than in normal lung tissue, based on the pooled OR from nine studies including 532 NSCLC and 372 normal lung tissue samples (OR=8.19, 95% confidence interval [CI]=5.41–12.39, P,0.00001). T-cadherin hypermethylation may also be associated with pathological types. The pooled OR was obtained from four studies including 111patients with squamous cell carcinoma and 106 with adenocarcinoma (OR=0.35, 95% CI=0.19–0.66, P=0.001), which indicated that T-cadherin hypermethylation plays a more important role in the pathogenesis of adenocarcinoma. We did not find that T-cadherin hypermethylation was correlated with the sex or smoking status, clinical stages, or epidermal growth factor receptor (EGFR) mutation status. However, T-cadherin hypermethylation was found to be significantly higher in poorly differentiated NSCLC than in moderately and highly differentiated NSCLC, and NSCLC patients with T-cadherin hypermethylation had a lower survival rate than those without T-cadherin hypermethylation.Conclusion: The results of this meta-analysis suggest that T-cadherin hypermethylation is associated with an increased risk and worse survival in NSCLC. T-cadherin hypermethylation, which induces the inactivation of T-cadherin gene, plays an important role in the carcinogenesis, cancer progression, as well as clinical outcome. © 2015 Wang et al.
Hong X.,Central South University |
Hong X.,Hunan Geriatric Hospital |
Ward P.,Flinders University |
Woodman R.J.,Flinders University |
And 3 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2017
Patients with hypertension and elevated homocysteine levels have a higher prevalence of risk factors for cardiovascular diseases (CVD) than individuals without either hypertension or elevated homocysteine levels, but the independence of homocysteine as a predictor of hypertension and vice-versa is controversial. This study was to explore the independence of hypertension and homocysteine (HCY) associations using a case-control study design based on a hospital in China. A case-control study was performed using 230 randomly selected 65-year-old and older inpatients with hypertension (cases) and 230 age and sex matched non-hypertensive controls. All participants were assessed for fasting triglycerides (TG), total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL), blood urea nitrogen (BUN), creatinine (CR), uric acid (UA), C-reactive protein (CRP), fasting glucose (BS), aspartate aminotransferase (AST) and body mass index (BMI). Data analysis was performed using conditional logistic regression. Compared to patients with hypertension, HCY, TG, TC, HDL, LDL, BUN, UA, CRP, BS and BMI were worse than controls. In multivariate analysis HCY (OR=1.22, P<0.001), BUN (OR=1.25, P=0.03) and CRP (OR=1.25, P=0.02) were independent predictors of hypertension. Amongst all subjects (cases and controls), the presence of elevated homocysteine was independently associated with hypertension (OR=9.93, P<0.001), TG (OR=3.56, P<0.001), LDL (OR=1.64, P=0.01), CRP (OR=1.50, P<0.001), BS (OR=0.75, P=0.02) and BMI (OR=1.14, P=0.001). Elevated homocysteine and hypertension are each independent predictors of each other. Hypertension is also independently associated with BUN and CRP, while elevated homocysteine is independently associated with TG, TC, LDL, CRP, BS and BMI. © 2017, E-Century Publishing Corporation. All rights reserved.
Zhu L.-M.,Central South University |
Zhu L.-M.,Hunan Geriatric Hospital |
Liu C.-H.,Central South University |
Chen P.,Central South University |
And 6 more authors.
International Journal of Tuberculosis and Lung Disease | Year: 2011
SETTING: Human neutrophil peptides 1, 2 and 3 (HNP1-3) are involved in innate host defence and acquired immune response, which is possibly associated with the genesis of multidrug-resistant tuberculosis (MDR-TB). OBJECTIVE: To investigate the relationship between HNP1-3 and MDR-TB. DESIGN: We divided 42 patients with post-primary pulmo nary TB into two groups according to their drug susceptibility test results: the drug-susceptible group (n = 21) and the MDR-TB group (n = 21). The concentration of HNP1-3 in the plasma of all specimens was measured by enzyme-linked immunosorbent assay before treatment and 6, 18 and 24 months after. Duration of Mycobacterium tuberculosis in sputum and peripheral blood neutrophil counts were measured at the same time. RESULTS: Before treatment, the plasma HNP1-3 concentration in the MDR-TB group was lower than that of healthy controls and the drug-susceptible group. After treatment, plasma HNP1-3 concentrations were higher than pre-treatment levels in the MDR-TB group, but were still lower than in healthy controls or the drug-susceptible group. The concentration of HNP1-3 was negatively correlated with the duration of M. tuberculosis in sputum, while it was positively correlated with neutrophils. CONCLUSION: MDR-TB is associated with low plasma concentrations of HNP1-3. © 2011 The Union.
Xiao K.,Central South University |
Jiang J.,Changsha Central Hospital |
Wang W.,Central South University |
Cao S.,Central South University |
And 5 more authors.
Oncology Reports | Year: 2013
Sirt3, a member of the mammalian sirtuin family protein that is localized to mitochondria, is a NAD+-dependent deacetylase and plays an important role in the control of metabolic activity. Recently, several studies have shown the potential role of Sirt3 in certain types of tumors such as breast cancer and hepatocellular carcinoma. However, the role of Sirt3 in lung adenocarcinoma has never been studied. In the present study, we found that Sirt3 protein expression was downregulated in human lung adenocarcinoma tissue when compared with that in adjacent normal tissue. Overexpression of Sirt3 using adenovirus significantly inhibited the growth of the A549 lung adenocarcinoma cell line. In this cell line, overexpression of Sirt3 induced apoptosis, which was evidenced by Annexin V + PI assay and cleaved caspase-3 immunoblotting. Furthermore, overexpression of Sirt3 increased the bax/bcl-2 and bad/bcl-x/L ratios, and promoted AIF translocation to the nucleus. Finally, Sirt3 overexpression upregulated p53 and p21 protein levels, and decreased intracellular ROS levels. Collectively, our data suggest that Sirt3 is a tumor suppressor in lung adenocarcinoma development and progression and may be a promising therapeutic target for lung adenocarcinoma.
Zeng F.-R.,Southern Medical University |
Zeng F.-R.,Hunan Geriatric Hospital |
Ling Y.,Hunan Geriatric Hospital |
Yang J.,Hunan Geriatric Hospital |
And 3 more authors.
Tumor Biology | Year: 2013
X-ray repair cross-complementing group 1 (XRCC1), a DNA repair enzyme, plays a crucial role in the base excision repair by generating a single nucleotide repair patch. It has been demonstrated that the XRCC1 Arg399Gln gene polymorphism was associated with variations in XRCC1 enzyme activity. The aim of this study was to quantitatively summarize the association between the XRCC1 Arg399Gln polymorphism and susceptibility to colorectal cancer (CRC). A comprehensive search of the PubMed, Embase, and China National Knowledge Infrastructure databases was conducted for studies on the association between the XRCC1 Arg399Gln polymorphism and CRC risk. Summary odds ratio (OR) with its corresponding 95 % confidence interval (95 %CI) was estimated, in a fixed-effects model or a random-effects model when appropriate, to assess the association. Totally, 26 case-control studies with 6,979 cases and 11,470 controls were included into this meta-analysis. The pooled results of total studies showed that the XRCC1 Arg399Gln polymorphism was significantly associated with increased risk of CRC in all genetic contrast models (OR A vs. G = 1.13, 95 %CI 1.03-1.23, P OR = 0.008; OR Gln/Gln vs. Arg/Arg = 1.24, 95 %CI 1.04-1.46, P OR = 0.015; ORGln/Gln vs. Arg/Gln + Arg/Arg = 1.19, 95 %CI 1.03-1.38, P OR = 0.021; ORGln/Gln + Arg/Gln vs. Arg/Arg = 1.14, 95 %CI 1.02-1.28, P OR = 0.022), except for the additive contrast model (ORArg/Gln vs. Arg/Arg = 1.11, 95 %CI 0.99-1.25, P OR = 0.064). The statistically significant association between the XRCC1 Arg399Gln polymorphism and CRC risk was observed among studies with high quality and in Asians, but not in Caucasians. Sensitivity analyses by sequential omission of any individual studies further identified the significant association. Publication bias was inexistent in this meta-analysis. The meta-analysis suggests that the XRCC1 Arg399Gln polymorphism is associated with increased risk of CRC. © 2012 International Society of Oncology and BioMarkers (ISOBM).
PubMed | University of South Australia, Hunan Geriatric Hospital, University of New South Wales and Central South University
Type: Journal Article | Journal: Clinical and experimental hypertension (New York, N.Y. : 1993) | Year: 2016
Hypertension is a major risk factor for cardiovascular diseases in China; hence, identifying good serum markers might provide cost benefits in terms of reducing morbidity rates. In this population-based case-control study, participants were recruited from five districts in Hunan province, and 416 cases were matched with an equal number of controls. Markers related to elevated blood pressure were assessed: Body Mass Index, total cholesterol, triglycerides, fasting blood glucose, and creatinine. Three potential serum markers homocysteine (HCY), C-reactive protein (CRP), and alanine aminotransferase (ALT) were dichotomized as normal or high level. Binary logistic regression was used to determine odds ratios (ORs) and 95% confidence intervals (CIs). The findings showed that ALT is a powerful serum marker for predicting high risk of high blood pressure with OR = 2.94, 95% CI (1.44-6.02), while there were no significant differences between cases and controls for HCY and CRP. Additionally, it seems likely that high concentrations of HCY conferred a protective effect against elevated blood pressure. When adjusted for sex, ORs for hypertensive females were nearly five times higher than for hypertensive males (OR = 4.34, 95% CI = 1.17-16.04). The study strongly supports findings showing ALT is a potential indicator for patients with hypertension.
Jia W.,Hunan Geriatric Hospital |
Shilling W.,Hunan Geriatric Hospital |
Dan Z.,Hunan Geriatric Hospital |
Na Z.,Hunan Geriatric Hospital |
Xiuqin H.,Hunan Geriatric Hospital
Chinese Journal of Cerebrovascular Diseases | Year: 2015
Objective To investigate the relationship between methylenetetrahydrofotate reductase (MTHFR) C677T polymorphisms and H-type hypertension and increased plasma homocysteine ( Hey) levels. Methods From September 2013 to June 2014,4 012 permanent residents aged 30year from 12 natural villages or communities in 6 regions of Hunan province were extracted according to the cluster random sampling method. Using computer random number table, 571 residents were randomly selected as the research objects. According to the blood pressure and Hey levels,571 residents were divided into 3 groups;a common hypertension group ( n = 190), an H-type hypertension group (n =94) , and a normal blood pressure group ( n = 287 ). Amplification refractory mutation systein-polymerase chain reaction (ARMS-PCR) method was used to detect the MTHFR C677T polymorphisms in all the research objects and the penotyping was performed. Hey levels were detected at the same time. Results There were significant differences in recessive model (CC + CT,TT) genotype frequencies among the H-type hypertension group (n = 66[70.2% ],n = 28[ 29. 8% ]),common hypertension group (n = 156[ 82.1 % ],n = 34[ 17.9% ]), and normal blood pressure group (n = 235 [ 81. 9% ] , n = 52 [ 18. 1%]) = 6. 797 ,P = 0. 033) , and there were no significant differences in CC,CT,and TT genotype frequencies among the 3 groups (P > 0.05). In the recessive model, there were significant differences in TT genotype frequencies between the H-type hypertension group and the normal blood pressure group or the common hypertension group ( χ2 = 5.812,P = 0.016;χ 2 = 5. 212,P = 0.022). There was no significant difference in TT genotype frequencies between the common hypertension group and the normal blood pressure group ( P > 0. 05 ). The CC + CT and TT genotype Hey levels of the MTHFR C677T recessive model in the H-type hypertension group were 17.1 ±1.6 and 19.0 ±2.9 (xmol/L respectively. There was significant difference between the genotypes (t= - 3. 115, P = 0.004). The logistic regression analysis of MTHFR C677T recessive model genotype showed that after adjusting for sex and age, the residents with recessive model TT genotype had higher risk of H-type hypertension (OR, 1. 946,95% CI 1. 172-3. 232, P = 0. 01). Conclusion The TT MTHFR C677T gene mutation in this population may be an important genetic factor for the increased Hey levels and the onset of H-type hypertension.
Liu X.Y.,Hunan Geriatric Hospital
Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology | Year: 2012
To study the expression of lung Krüppel-like transcription factor (KLF2/LKLF) in lung tissues of rats with chronic obstructive pulmonary disease (COPD) and the relationship between KLF2 and NF-E2-related factor 2 (Nrf2), and make further explore the effects of KLF2 on the expression of gamma-glutamylcysteine synthetase (gamma-GCS). Twenty-two male SD rats were randomly divided into a COPD group (n = 10) and a normal control group (n = 11). The rat model of COPD established by cigarette smoking and intratracheal instillation of lipopolysaccharide (LPS), and lung tissues were obtained. The expressions of KLF2, Nrf2, gamma-GCS mRNA and protein in lung tissues were measured by immunohistochemistry (IHC), Western blot, in situ hybridization (ISH) and reverse transcription-polymerase chain reaction (RT-PCR). To explore the relationship between KLF2 and Nrf2 protein,we utilize the method of co-immunoprecipitation (CO-IP). IHC and Western blot showed that protein expressions of KLF2, Nrf2, gamma-GCS were higher in the lung tissues from rats with COPD than those in the control groups (all P < 0.05). The levels of KLF2, gamma-GCS mRNA were markedly increased in the COPD group (all P < 0.01) while Nrf2 mRNA expression in COPD group had no significant difference with that in control group ( P > 0.05). CO-IP result showed that KLF2 were obviously present in immunoprecipitates of Nrf2 (P < 0.01) . Linear correlation analysis showed that the level of KLF2 protein was positively correlated with the level of Nrf2 protein (P < 0.05), and KLF2, Nrf2 proteins were positively correlated with gamma-GCS mRNA and protein (all P < 0.05). The expression of KLF2 is significantly up-regulated in COPD, which maybe up-regulate gamma-GCS mRNA expression by increasing Nrf2 expression and nuclear translocation against oxidative stress.
PubMed | Hunan Geriatric Hospital
Type: Journal Article | Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology | Year: 2012
To study the expression of lung Krppel-like transcription factor (KLF2/LKLF) in lung tissues of rats with chronic obstructive pulmonary disease (COPD) and the relationship between KLF2 and NF-E2-related factor 2 (Nrf2), and make further explore the effects of KLF2 on the expression of gamma-glutamylcysteine synthetase (gamma-GCS).Twenty-two male SD rats were randomly divided into a COPD group (n = 10) and a normal control group (n = 11). The rat model of COPD established by cigarette smoking and intratracheal instillation of lipopolysaccharide (LPS), and lung tissues were obtained. The expressions of KLF2, Nrf2, gamma-GCS mRNA and protein in lung tissues were measured by immunohistochemistry (IHC), Western blot, in situ hybridization (ISH) and reverse transcription-polymerase chain reaction (RT-PCR). To explore the relationship between KLF2 and Nrf2 protein,we utilize the method of co-immunoprecipitation (CO-IP).IHC and Western blot showed that protein expressions of KLF2, Nrf2, gamma-GCS were higher in the lung tissues from rats with COPD than those in the control groups (all P < 0.05). The levels of KLF2, gamma-GCS mRNA were markedly increased in the COPD group (all P < 0.01) while Nrf2 mRNA expression in COPD group had no significant difference with that in control group ( P > 0.05). CO-IP result showed that KLF2 were obviously present in immunoprecipitates of Nrf2 (P < 0.01) . Linear correlation analysis showed that the level of KLF2 protein was positively correlated with the level of Nrf2 protein (P < 0.05), and KLF2, Nrf2 proteins were positively correlated with gamma-GCS mRNA and protein (all P < 0.05).The expression of KLF2 is significantly up-regulated in COPD, which maybe up-regulate gamma-GCS mRNA expression by increasing Nrf2 expression and nuclear translocation against oxidative stress.
PubMed | Hunan Geriatric Hospital
Type: Journal Article | Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology | Year: 2010
To elucidate the location and effects of transcription factor-nuclear factor-kappaB (NF-kappaB) in lung tissues of rats with chronic obstructive pulmonary disease (COPD).Fourteen male Wistar rats were randomly divided into COPD model and control groups equally. The COPD model was established by intratracheal instillation of lipopolysaccharide twice and exposure to cigarette smoke daily. We detected the NF-kappaB p65 protein in lung by immunohistochemical method, and the expression of NF-kappaB p65 mRNA in lung by in situ hybridization.Immunohistochemistry, the expression of NF-kappaB p65 protein in alveolar, bronchiolar epithelium and arteriolar endothelium was significantly higher in the COPD group (0.426 +/- 0.007, 0.434 +/- 0.012 and 0.313 +/- 0.007, respectively) than those of the control group (0.115 +/- 0.006, 0.116 +/- 0.005 and 0.095 +/- 0.007, respectively, all P < 0.01). In situ hybridization showed that the expressions of NF-kappaB p65 mRNA in alveolar epithelium (0.203 +/- 0.008), bronchiolar and arteriolar smooth muscle cell (0.208 + 0. 010 and 0.206 + 0.007) of rats in the COPD group were stronger than those in the control group (0.100 +/- 0.006, 0.102 +/- 0.002 and 0.103 +/- 0.003 respectively) by semiquantitative analysis (all P < 0.01).The expression and nuclear translocation of NF-kappaB may be the basis event of gene expression of many cytokines and inflammatory mediators, which may positively regulate gene expression of many cytokines and inflammatory mediators in various cell lines.