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Song R.,CAS Wuhan Institute of Hydrobiology | Song R.,Hunan Fisheries Science Institute | Song R.,University of Chinese Academy of Sciences | Li W.X.,CAS Wuhan Institute of Hydrobiology | And 4 more authors.
Journal of Parasitology

The acanthocephalan Acanthosentis cheni was found in anadromous, freshwater, and landlocked stocks of its fish host, Coilia nasus. To examine the genetic variations of the acanthocephalan among the 3 populations with the adaptation of the host to the freshwater, the genetic structure of the helminth was investigated in anadromous (Zhoushan and Chongming islands, and Anqing), freshwater (Anqing, Ezhou, and Poyang Lake), and landlocked (Tian'ezhou Reserve) populations by sequencing intergenic transcribed spacers (ITS) of the ribosomal RNA coding genes. Low Fst values and high gene flow were found among the 7 populations (Fst = 0.0135, P = 0.2723; Nm = 36.48) and the 3 ecotypes of Acanthosentis cheni (Fst = 0.0178, P = 0.1044; Nm = 27.67). On the other hand, significant genetic differentiation of the C. nasus host populations was detected between the upstream and downstream areas of Xiaogu Mountain (Fst = 0.1961, P = 0.0030; Nm = 2.05), which is the farthest location of spawning migration for C. nasus. However, the migration break of the fish host appeared not to cause significant genetic differentiation of A. cheni populations between the upper and lower reaches of Xiaogu Mountain. Other factors might promote genetic exchange of A. cheni populations such as dispersal of the intermediate host by flooding or other fish species serving as the definitive or paratenic hosts. In Anqing, nucleotide diversity of the acanthocephalan was highest in the freshwater population (0.0038) and lower in the anadromous population (0.0026). This suggested that new mutations may have occurred in the freshwater A. cheni population in Anqing when adapting to a freshwater environment. © American Society of Parasitologists 2014. Source

Chu W.Y.,Changsha University | Liu X.L.,Changsha University | Liu X.L.,Guangxi Normal University | Chen D.X.,Changsha University | And 6 more authors.
Aquaculture Nutrition

Grass carp (Ctenopharyngodon idella) is a valuable aquacultural species in China, but it is highly vulnerable to infectious diseases, which consequently may cause tremendous economical loss. To reduce the risk of disease in the fish, antibiotics have been used in the fish diet, which in turn caused some negative effects on human health. In this study, we choose lactosucrose (LS) as antibiotic alternative added to the diet of the grass carp juveniles and then to evaluate the effects of 15 g Kg-1 LS in the diet on the gene transcript profiles. After the trial for 56 days, we observed that the weight gain rate, specific gain rate and feed conversion rate were increased by 14.2%, 9.2% and 10.1%, respectively, in the trial group compared with the control group. The gene expression levels in the liver tissues of grass carps were assayed using the zebrafish cDNA microarray technology and real-time RT-PCR. Comparing with the control group, 416 genes were identified, and among them, 266 genes were up-regulated and 150 were down-regulated on the trial group. Among the up-regulated genes selected, most of them related to growth, immune reaction and sugar metabolism. Most of the down-regulated genes are RAS oncogene family members, V-myc viral oncogene homologue and programmed cell death-related factors. The apparent regulation of gene expression stimulated by LS suggests that the potential application of the LS in improving the growth performance and immunity on the grass carps. Together, this study provides convincing data in support of the use of LS as an alternative dietary antibiotic in fish. © 2013 John Wiley & Sons Ltd. Source

Liu Y.,Chinese Academy of Fishery Sciences | Liu Y.,Huazhong Agricultural University | Liu Y.,Hubei Freshwater Aquaculture Collaborative Innovation Center | Ai X.,Chinese Academy of Fishery Sciences | And 7 more authors.
Analytical Letters

Niclosamide is a mollusicide used for aquaculture on a large scale in China. However, it has high toxicity to aquatic animals and may cause damage to DNA. A modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction procedure with high-performance liquid chromatography–heated electrospray ionization-tandem mass spectrometry method was developed for its determination. Samples were extracted with ammoniated acetonitrile, and anhydrous magnesium sulfate was added to remove moisture and precipitate proteins. The extracts were further purified with octadecylsilane. The recoveries of niclosamide in fortified muscle tissue at concentrations of 0.5 to 20 µg/kg were between 76.40% and 95.60% with relative standard deviations less than 9.54%. The limits of detection and quantification were 0.2 µg/kg and 0.5 µg/kg, respectively. The developed method was used to study the adsorption of niclosamide in channel catfish after administration. © 2015, Yangtze River Fisheries Research Institute. Source

Zeng C.-F.,Hunan Fisheries Science Institute | Li X.-L.,Hunan Fisheries Science Institute | Li C.-W.,Hunan Fisheries Science Institute | Huang X.-R.,Hunan Fisheries Science Institute | Wan Y.-W.,Hunan Fisheries Science Institute
Mitochondrial DNA

The complete mitochondrial genome sequence of Onychostoma rara was determined to be 16,590 bp in length and contains 13 protein-coding genes (PCGs), 22 tRNA genes, large (rrnL) and small (rrnS) rRNA and the non-coding control region. Its total A + T content is 55.65%. We also analyzed the structure of control region, 6 CSBs (CSB-1, CSB-2, CSB-3, CSB-D, CSB-E and CSB-F) and 2 bp tandem repeat were detected. © 2014 Informa UK Ltd. Source

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