Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients

Changsha, China

Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients

Changsha, China
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Chen S.,Hunan Agricultural University | Jiang H.,Hunan Agricultural University | Peng H.,Hunan Agricultural University | Wu X.,Hunan Agricultural University | And 2 more authors.
Oxidative Medicine and Cellular Longevity | Year: 2017

Several of the most prevalent etiological factors which contribute towards global death rates are associated with cardiovascular diseases (CVDs), which include a range of conditions such as angina, rheumatic heart disease, and venous thrombosis. Extensive research has been conducted into the role played by oxidative stress and inflammation in the functional transformations associated with the progression of CVDs, while the research findings from these investigations have been both fruitful and informative. In view of the adverse secondary effects that result from the clinical administration of many synthetic medications, research which explored the treatment of severe and long-lasting conditions, including CVDs, has primarily centered on the potential benefits displayed by natural agents, one of which is food protein-based bioactive peptides. Most importantly, previous research has revealed the possible benefits associated with these products' anti-inflammatory and antioxidant characteristics. In light of these considerations, this paper aims to review the degree to which ovotransferrin (otrf, also referred to as conalbumin) and otrf-derived peptides, including IRW, IQW, and KVREGT, are, by virtue of their anti-inflammatory and antioxidant characteristics, viable treatment agents for endothelial dysfunction and the prevention of CVD. © 2017 Shuang Chen et al.


Yuan Z.-H.,Hunan Agricultural University | Yuan Z.-H.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Wu J.,Hunan Agricultural University | Wu J.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | And 4 more authors.
Molecules (Basel, Switzerland) | Year: 2016

Koumine is a kind of alkaloid extracted from Gelsemium elegans (G. elegans). Benth, which has shown promise as an anti-tumor, anxiolytic, and analgesic agent. In our present study, the effect of koumine on lipopolysaccharide (LPS)-mediated RAW 264.7 cell apoptosis was evaluated. MTT assays showed that koumine obviously increased cell viability in LPS-mediated RAW 264.7 macrophages. Preincubation with koumine ameliorated LPS-medicated apoptosis by decreasing reactive oxygen species (ROS) production, which resulted in a significant decrease in the levels of nitric oxide (NO) and inducible nitric oxide synthase (iNOS). In addition, koumine-pretreated RAW 264.7 macrophages exhibited reduction of LPS-induced levels of TNF-α, IL-1β, and IL-6 mRNA. Furthermore, pretreatment with koumine suppressed LPS-mediated p53 activation, loss of mitochondrial membrane potential, caspase-3 activation, decrease of Bcl-2 expression, and elevation of Bax and caspase-3 expressions, suggesting that koumine might act directly on RAW 264.7 cells to inhibit LPS-induced apoptosis. It seems as though the mechanism that koumine possesses is the anti-apoptotic effect mediated by suppressing production of ROS, activation of p53, and mitochondrial apoptotic pathways in RAW 264 cells. Koumine could potentially serve as a protective effect against LPS-induced apoptosis.


Su Y.,Hunan Agricultural University | Luo W.,Hunan Agricultural University | Chen X.,Hunan Agricultural University | Liu H.,Hunan Agricultural University | And 5 more authors.
Biological Procedures Online | Year: 2017

Background: Indole-3-acetic acid (IAA) extraction and purification are of great importance in auxin research, which is a hot topic in the plant growth and development field. Solid-phase extraction (SPE) is frequently used for IAA extraction and purification. However, no IAA-specific SPE columns are commercially available at the moment. Therefore, the development of IAA-specific recognition materials and IAA extraction and purification methods will help researchers meet the need for more precise analytical methods for research on phytohormones. Results: Since the AUXIN RESISTANT/INDOLE-3-ACETIC ACID INDUCIBLE (Aux/IAA) proteins show higher specific binding capability with auxin, recombinant IAA1, IAA7 and IAA28 proteins were used as sorbents to develop an IAA extraction and purification method. A GST tag was used to solidify the recombinant protein in a column. Aux/IAA proteins solidified in a column have successfully trapped trace IAA in aqueous solutions. The IAA7 protein showed higher IAA binding capability than the other proteins tested. In addition, expression of the IAA7 protein in Drosophila Schneider 2 (S2) cells produced better levels of binding than IAA7 expressed in E. coli. Conclusion: This work validated the potential of Aux/IAA proteins to extract and purify IAA from crude plant extracts once we refined the techniques for these processes. © 2017 The Author(s).


Yuan Z.-H.,Hunan Agricultural University | Yuan Z.-H.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Wu J.,Hunan Agricultural University | Wu J.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | And 6 more authors.
Molecules | Year: 2016

Koumine is a kind of alkaloid extracted from Gelsemium elegans (G. elegans). Benth, which has shown promise as an anti-Tumor, anxiolytic, and analgesic agent. In our present study, the effect of koumine on lipopolysaccharide (LPS)-mediated RAW 264.7 cell apoptosis was evaluated. MTT assays showed that koumine obviously increased cell viability in LPS-mediated RAW 264.7 macrophages. Preincubation with koumine ameliorated LPS-medicated apoptosis by decreasing reactive oxygen species (ROS) production, which resulted in a significant decrease in the levels of nitric oxide (NO) and inducible nitric oxide synthase (iNOS). In addition, koumine-pretreated RAW 264.7 macrophages exhibited reduction of LPS-induced levels of TNF-α, IL-1β, and IL-6 mRNA. Furthermore, pretreatment with koumine suppressed LPS-mediated p53 activation, loss of mitochondrial membrane potential, caspase-3 activation, decrease of Bcl-2 expression, and elevation of Bax and caspase-3 expressions, suggesting that koumine might act directly on RAW 264.7 cells to inhibit LPS-induced apoptosis. It seems as though the mechanism that koumine possesses is the anti-Apoptotic effect mediated by suppressing production of ROS, activation of p53, and mitochondrial apoptotic pathways in RAW 264 cells. Koumine could potentially serve as a protective effect against LPS-induced apoptosis. © 2016 by the authors; licensee MDPI, Basel, Switzerland.


Xiao H.-B.,Hunan Agricultural University | Lu X.-Y.,Hunan Agricultural University | Lu X.-Y.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Liu Z.-K.,Hunan Agricultural University | And 2 more authors.
Journal of Physiology and Biochemistry | Year: 2016

In the present study, we tested the hypothesis that aldosterone regulates osteopontin (OPN)-related signaling pathways to promote nuclear factor κB (NF-κB) activation in primary human umbilical vein endothelial cells (HUVECs) and that kaempferol, a flavonoid compound, blocks those changes. Aldosterone induced productions of reactive oxygen species (ROS), OPN, interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α) and expression of nicotinamide adenine dinucleotide phosphate-oxidase 4 (Nox4), NF-κB, OPN, alphavbeta3 (αvβ3) integrin, and inhibitor of NF-κB alpha phosphorylation (P-IκBα) in HUVEC. HUVECs were pretreated with kaempferol (0, 1, 3, or 10 μM) for 1 h and exposed to aldosterone (10−6 M) for 24 h. Kaempferol reduced ROS, OPN, NF-κB, IL-6, and TNF-α levels; Nox4, αvβ3 integrin; and P-IκBα expressions. The effect of aldosterone was also abrogated by spironolactone (10−6 M). In addition, vitamin C (20 mmol/L) reduced ROS production. Vitamin C and LM609 (10 μg/mL) treatment decreased expressions of OPN, αvβ3 integrin, and NF-κB (P < 0.05 or P < 0.01). The present results suggest that kaempferol may modulate OPN–αvβ3 integrin pathway to inhibit NF-κB activation in HUVECs. © 2016 University of Navarra


Ding W.,Hunan Agricultural University | Ding W.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Li Y.,Hunan Agricultural University | Li G.,Hunan Agricultural University | And 3 more authors.
Molecules | Year: 2016

Three new 30-noroleanane triterpenoid saponins, akebonoic acid 28-O-β-D-glucopyranosyl-(1″→6′)-β-D-glucopyranosyl ester (1), akebonoic acid 28-O-(6″-O-caffeoyl)-β-D-glucopyranosyl-(1″→6′)-β-D-glucopyranosyl ester (Holboelliside A, 2) and 3β,20α,24-trihydroxy-29-norolean-12-en-28-oic acid 3-O-(6′-O-caffeoyl)-β-D-glucopyranoside (Holboelliside B, 3) were isolated from the stems of Holboellia coriacea Diels, together with five known compounds, eupteleasaponin VIII (4), 3α-akebonoic acid (5), quinatic acid (6), 3β-hydroxy-30-norhederagenin (7) and quinatoside A (8). The structures of these compounds were determined on the basis of spectral and chemical evidence. Compounds 1-5 were evaluated for their inhibitory activity against three human tumors HepG2, HCT116 and SGC-7901 cell lines in vitro. © 2016 by the authors; licensee MDPI.


Su Y.,Hunan Agricultural University | Su Y.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Luo W.,Hunan Agricultural University | Zhao X.,Hunan Agricultural University | And 4 more authors.
Plant and Soil | Year: 2015

Aims: KUP/HAK/KT transporters mainly play a role of K+ uptake in plants. PaHAK1 was a previously isolated KUP/HAK/KT transporter gene from a high K+ plant Phytolacca acinosa Roxb. In this study, we want to identify the function of PaHAK1. Methods: PaHAK1 was overexpressed in eukaryotes including Arabidopsis, rice, and yeast. The growth and K+ uptake of transgenic lines in media of low K+ concentration were investigated. Results: We found that PaHAK1 rescued the growth phenotype of athak5 seedlings which was hard to grow in the media of 10 μM K+. Faster depletion from diluted K solutions was detected for transgenic than for WT plants. Moreover, PaHAK1 confers the growth of K+ uptake deletion yeast mutant on AP media containing 50 μM of K+. Conclusions: Taken together, these results suggest an important role for PaHAK1 transporter on K+ uptake. © 2015 Springer International Publishing Switzerland


Li G.,Hunan Agricultural University | Ding W.,Hunan Agricultural University | Ding W.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Wan F.,Hunan Agricultural University | And 3 more authors.
Molecules | Year: 2016

Two new clerodane-type diterpenes, tinosporins C (1) and tinosporins D (2) were isolated from the stems of Tinospora sagittata (Oliv.), together with three known ones, columbin (3), tinophylloloside (4), and tinospinoside D (5). The structures of these compounds were determined on the basis of spectroscopic data interpretation, with that of the absolute configuration of compound 1 was assigned by experimental and calculated ECD spectra. The cytotoxicity and α-glucosidase inhibitory activities of isolated compounds were evaluated in vitro. © 2016 by the authors; licensee MDPI.


Su Y.,Hunan Agricultural University | Su Y.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Luo W.,Hunan Agricultural University | Lin W.,Hunan Agricultural University | And 3 more authors.
Biological Procedures Online | Year: 2015

Trk/Ktr/HKT transporters probably were evolved from simple K+ channels KcsA. HKT transporters, which mediate Na+-uniport or Na+/K+-symport, maintain K+/Na+ homeostasis and increase salinity tolerance, can be classified into three subfamilies in higher plants. In this review, we systematically analyzed the characteristics of amino acids sequences and physiological functions of HKT transporters in higher plant. Furthermore, we depicted the hypothetical models of cations selection and transportation mediated by HKT transporters according to the highly conserved structure for the goal of better understanding the cations transportation processes. © 2015 Su et al.; licensee BioMed Central.


Liu X.,Hunan Agricultural University | Liu X.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Zheng S.,Hunan Agricultural University | Zheng S.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | And 8 more authors.
Food Analytical Methods | Year: 2016

In this paper, a low-cost, simple, and highly sensitive electrochemical immunosensor was fabricated for the tetracycline detection based on gold electrode-modified carboxyl-Fe3O4 nanoparticle (MNPs) by chitosan (CS) as linker. The anti-tetracycline monoclonal antibody (Ab) was immobilized on the modified electrode surface. The binding of tetracycline to Ab was analyzed by differential pulse voltammetry. Here, MNPs were used as the signal amplifier to improve the sensitivity of the immunosensor. The stepwise assembly process of the electrochemical immunosensor was characterized by cyclic voltammetry and electrochemical impedance spectroscopy. Under the optimum operating conditions, the fabricated immunosensor showed a linear current response to the target concentration in the range from 0.08 to 1 ng/mL with a lower detection limit of 0.0321 ng/mL (S/N = 3). It was successfully applied to the detection of tetracycline in milk. Enzyme-linked immunoassay analysis was also conducted to detect tetracycline in the same samples for demonstrating the applicability of the electrochemical immunosensor. © 2016 Springer Science+Business Media New York

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