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Xiao H.-B.,Hunan Agricultural University | Lu X.-Y.,Hunan Agricultural University | Lu X.-Y.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Liu Z.-K.,Hunan Agricultural University | Luo Z.-F.,Xiangnan University
Journal of Physiology and Biochemistry | Year: 2016

In the present study, we tested the hypothesis that aldosterone regulates osteopontin (OPN)-related signaling pathways to promote nuclear factor κB (NF-κB) activation in primary human umbilical vein endothelial cells (HUVECs) and that kaempferol, a flavonoid compound, blocks those changes. Aldosterone induced productions of reactive oxygen species (ROS), OPN, interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α) and expression of nicotinamide adenine dinucleotide phosphate-oxidase 4 (Nox4), NF-κB, OPN, alphavbeta3 (αvβ3) integrin, and inhibitor of NF-κB alpha phosphorylation (P-IκBα) in HUVEC. HUVECs were pretreated with kaempferol (0, 1, 3, or 10 μM) for 1 h and exposed to aldosterone (10−6 M) for 24 h. Kaempferol reduced ROS, OPN, NF-κB, IL-6, and TNF-α levels; Nox4, αvβ3 integrin; and P-IκBα expressions. The effect of aldosterone was also abrogated by spironolactone (10−6 M). In addition, vitamin C (20 mmol/L) reduced ROS production. Vitamin C and LM609 (10 μg/mL) treatment decreased expressions of OPN, αvβ3 integrin, and NF-κB (P < 0.05 or P < 0.01). The present results suggest that kaempferol may modulate OPN–αvβ3 integrin pathway to inhibit NF-κB activation in HUVECs. © 2016 University of Navarra

Su Y.,Hunan Agricultural University | Su Y.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Luo W.,Hunan Agricultural University | Zhao X.,Hunan Agricultural University | And 4 more authors.
Plant and Soil | Year: 2015

Aims: KUP/HAK/KT transporters mainly play a role of K+ uptake in plants. PaHAK1 was a previously isolated KUP/HAK/KT transporter gene from a high K+ plant Phytolacca acinosa Roxb. In this study, we want to identify the function of PaHAK1. Methods: PaHAK1 was overexpressed in eukaryotes including Arabidopsis, rice, and yeast. The growth and K+ uptake of transgenic lines in media of low K+ concentration were investigated. Results: We found that PaHAK1 rescued the growth phenotype of athak5 seedlings which was hard to grow in the media of 10 μM K+. Faster depletion from diluted K solutions was detected for transgenic than for WT plants. Moreover, PaHAK1 confers the growth of K+ uptake deletion yeast mutant on AP media containing 50 μM of K+. Conclusions: Taken together, these results suggest an important role for PaHAK1 transporter on K+ uptake. © 2015 Springer International Publishing Switzerland

Su Y.,Hunan Agricultural University | Su Y.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Luo W.,Hunan Agricultural University | Lin W.,Hunan Agricultural University | And 3 more authors.
Biological Procedures Online | Year: 2015

Trk/Ktr/HKT transporters probably were evolved from simple K+ channels KcsA. HKT transporters, which mediate Na+-uniport or Na+/K+-symport, maintain K+/Na+ homeostasis and increase salinity tolerance, can be classified into three subfamilies in higher plants. In this review, we systematically analyzed the characteristics of amino acids sequences and physiological functions of HKT transporters in higher plant. Furthermore, we depicted the hypothetical models of cations selection and transportation mediated by HKT transporters according to the highly conserved structure for the goal of better understanding the cations transportation processes. © 2015 Su et al.; licensee BioMed Central.

Ran T.,CAS Institute of Subtropical Agriculture | Ran T.,University of Chinese Academy of Sciences | Li H.,CAS Institute of Subtropical Agriculture | Li H.,University of Chinese Academy of Sciences | And 15 more authors.
Livestock Science | Year: 2016

In non-ruminants, sweet taste receptor and monosaccharide transporters are important components of nutrient chemosensing in the intestinal tracts. Ruminants, however, have evolved a polygastric digestive system linked to a transition in nutrient supply from readily-digestible carbohydrate (lactose) in milk during the pre-ruminant suckling phase to poorly-digestible structural carbohydrates when the rumen becomes functional. Whether these developmental and feed changes alter the mRNA expression of genes related to monosaccharide sensing (Taste receptor family 1 member 2, T1R2; Taste receptor family 1 member 3, T1R3) and transporting (monosaccharide transporters Na+/glucose co-transporter, SGLT1; glucose activated ion channel, SGLT3; solute carrier family 2 member 5, GLUT5; solute carrier family 2 member 2, GLUT2) are unknown. This study investigates the expression of sweet taste receptors (T1R2 and T1R3) and monosaccharides transporters (SGLT1, SGLT3, GLUT5 and GLUT2) along the gastrointestinal tracts (GIT) during different stages of development (suckling, weaning and grazing) in goats. The results showed that the expression of T1R2 decreased with age, and was mainly expressed in the duodenum and jejunum. Although T1R3 expression in different GIT segments fluctuated during growth, there was an increase with age, with the abomasum showing the greatest expression. Both SGLT1 and SGLT3 were mainly expressed in the jejunum at all ages, with the greatest expression in the middle jejunum during suckling, and decreased greatly (P<0.05) during weaning and grazing. GLUT5 was mainly expressed at the duodenum and jejunum, with differences (P<0.05) between suckling and the later developmental stages. No expression of GLUT5 was detected at the rumen and abomasum at any stage, but was noted in the ileum, cecum, colon and rectum during suckling, but with lower abundances during weaning and grazing. The expression of GLUT2 was detected only in the small intestine and decreased with age. The expression of T1R2 correlated (P<0.05) with SGLT1, SGLT3, GLUT5 and GLUT2. There were correlations (P<0.01) between the expressions of SGLT3 and SGLT1, as well as between SGLT3 and GLUT5. The current results indicate that: (1) the ability of the GIT of goats to absorb monosaccharides varies during development, being greatest at the suckling stage; (2) the duodenum and jejunum play a critical role in sensing and absorption of monosaccharides; (3) the mRNA expressions of sweet taste receptors and various transporters correlated well in the GIT of goats. © 2016 Elsevier B.V.

Ding W.,Hunan Agricultural University | Ding W.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Li Y.,Hunan Agricultural University | Li G.,Hunan Agricultural University | And 3 more authors.
Molecules | Year: 2016

Three new 30-noroleanane triterpenoid saponins, akebonoic acid 28-O-β-D-glucopyranosyl-(1″→6′)-β-D-glucopyranosyl ester (1), akebonoic acid 28-O-(6″-O-caffeoyl)-β-D-glucopyranosyl-(1″→6′)-β-D-glucopyranosyl ester (Holboelliside A, 2) and 3β,20α,24-trihydroxy-29-norolean-12-en-28-oic acid 3-O-(6′-O-caffeoyl)-β-D-glucopyranoside (Holboelliside B, 3) were isolated from the stems of Holboellia coriacea Diels, together with five known compounds, eupteleasaponin VIII (4), 3α-akebonoic acid (5), quinatic acid (6), 3β-hydroxy-30-norhederagenin (7) and quinatoside A (8). The structures of these compounds were determined on the basis of spectral and chemical evidence. Compounds 1-5 were evaluated for their inhibitory activity against three human tumors HepG2, HCT116 and SGC-7901 cell lines in vitro. © 2016 by the authors; licensee MDPI.

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