Time filter

Source Type

Yuan Z.-H.,Hunan Agricultural University | Yuan Z.-H.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Wu J.,Hunan Agricultural University | Wu J.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | And 6 more authors.
Molecules | Year: 2016

Koumine is a kind of alkaloid extracted from Gelsemium elegans (G. elegans). Benth, which has shown promise as an anti-Tumor, anxiolytic, and analgesic agent. In our present study, the effect of koumine on lipopolysaccharide (LPS)-mediated RAW 264.7 cell apoptosis was evaluated. MTT assays showed that koumine obviously increased cell viability in LPS-mediated RAW 264.7 macrophages. Preincubation with koumine ameliorated LPS-medicated apoptosis by decreasing reactive oxygen species (ROS) production, which resulted in a significant decrease in the levels of nitric oxide (NO) and inducible nitric oxide synthase (iNOS). In addition, koumine-pretreated RAW 264.7 macrophages exhibited reduction of LPS-induced levels of TNF-α, IL-1β, and IL-6 mRNA. Furthermore, pretreatment with koumine suppressed LPS-mediated p53 activation, loss of mitochondrial membrane potential, caspase-3 activation, decrease of Bcl-2 expression, and elevation of Bax and caspase-3 expressions, suggesting that koumine might act directly on RAW 264.7 cells to inhibit LPS-induced apoptosis. It seems as though the mechanism that koumine possesses is the anti-Apoptotic effect mediated by suppressing production of ROS, activation of p53, and mitochondrial apoptotic pathways in RAW 264 cells. Koumine could potentially serve as a protective effect against LPS-induced apoptosis. © 2016 by the authors; licensee MDPI, Basel, Switzerland.


Xiao H.-B.,Hunan Agricultural University | Lu X.-Y.,Hunan Agricultural University | Lu X.-Y.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Liu Z.-K.,Hunan Agricultural University | And 2 more authors.
Journal of Physiology and Biochemistry | Year: 2016

In the present study, we tested the hypothesis that aldosterone regulates osteopontin (OPN)-related signaling pathways to promote nuclear factor κB (NF-κB) activation in primary human umbilical vein endothelial cells (HUVECs) and that kaempferol, a flavonoid compound, blocks those changes. Aldosterone induced productions of reactive oxygen species (ROS), OPN, interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α) and expression of nicotinamide adenine dinucleotide phosphate-oxidase 4 (Nox4), NF-κB, OPN, alphavbeta3 (αvβ3) integrin, and inhibitor of NF-κB alpha phosphorylation (P-IκBα) in HUVEC. HUVECs were pretreated with kaempferol (0, 1, 3, or 10 μM) for 1 h and exposed to aldosterone (10−6 M) for 24 h. Kaempferol reduced ROS, OPN, NF-κB, IL-6, and TNF-α levels; Nox4, αvβ3 integrin; and P-IκBα expressions. The effect of aldosterone was also abrogated by spironolactone (10−6 M). In addition, vitamin C (20 mmol/L) reduced ROS production. Vitamin C and LM609 (10 μg/mL) treatment decreased expressions of OPN, αvβ3 integrin, and NF-κB (P < 0.05 or P < 0.01). The present results suggest that kaempferol may modulate OPN–αvβ3 integrin pathway to inhibit NF-κB activation in HUVECs. © 2016 University of Navarra


Ding W.,Hunan Agricultural University | Ding W.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Li Y.,Hunan Agricultural University | Li G.,Hunan Agricultural University | And 3 more authors.
Molecules | Year: 2016

Three new 30-noroleanane triterpenoid saponins, akebonoic acid 28-O-β-D-glucopyranosyl-(1″→6′)-β-D-glucopyranosyl ester (1), akebonoic acid 28-O-(6″-O-caffeoyl)-β-D-glucopyranosyl-(1″→6′)-β-D-glucopyranosyl ester (Holboelliside A, 2) and 3β,20α,24-trihydroxy-29-norolean-12-en-28-oic acid 3-O-(6′-O-caffeoyl)-β-D-glucopyranoside (Holboelliside B, 3) were isolated from the stems of Holboellia coriacea Diels, together with five known compounds, eupteleasaponin VIII (4), 3α-akebonoic acid (5), quinatic acid (6), 3β-hydroxy-30-norhederagenin (7) and quinatoside A (8). The structures of these compounds were determined on the basis of spectral and chemical evidence. Compounds 1-5 were evaluated for their inhibitory activity against three human tumors HepG2, HCT116 and SGC-7901 cell lines in vitro. © 2016 by the authors; licensee MDPI.


Su Y.,Hunan Agricultural University | Su Y.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Luo W.,Hunan Agricultural University | Zhao X.,Hunan Agricultural University | And 4 more authors.
Plant and Soil | Year: 2015

Aims: KUP/HAK/KT transporters mainly play a role of K+ uptake in plants. PaHAK1 was a previously isolated KUP/HAK/KT transporter gene from a high K+ plant Phytolacca acinosa Roxb. In this study, we want to identify the function of PaHAK1. Methods: PaHAK1 was overexpressed in eukaryotes including Arabidopsis, rice, and yeast. The growth and K+ uptake of transgenic lines in media of low K+ concentration were investigated. Results: We found that PaHAK1 rescued the growth phenotype of athak5 seedlings which was hard to grow in the media of 10 μM K+. Faster depletion from diluted K solutions was detected for transgenic than for WT plants. Moreover, PaHAK1 confers the growth of K+ uptake deletion yeast mutant on AP media containing 50 μM of K+. Conclusions: Taken together, these results suggest an important role for PaHAK1 transporter on K+ uptake. © 2015 Springer International Publishing Switzerland


Li G.,Hunan Agricultural University | Ding W.,Hunan Agricultural University | Ding W.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Wan F.,Hunan Agricultural University | And 3 more authors.
Molecules | Year: 2016

Two new clerodane-type diterpenes, tinosporins C (1) and tinosporins D (2) were isolated from the stems of Tinospora sagittata (Oliv.), together with three known ones, columbin (3), tinophylloloside (4), and tinospinoside D (5). The structures of these compounds were determined on the basis of spectroscopic data interpretation, with that of the absolute configuration of compound 1 was assigned by experimental and calculated ECD spectra. The cytotoxicity and α-glucosidase inhibitory activities of isolated compounds were evaluated in vitro. © 2016 by the authors; licensee MDPI.


Su Y.,Hunan Agricultural University | Su Y.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Luo W.,Hunan Agricultural University | Lin W.,Hunan Agricultural University | And 3 more authors.
Biological Procedures Online | Year: 2015

Trk/Ktr/HKT transporters probably were evolved from simple K+ channels KcsA. HKT transporters, which mediate Na+-uniport or Na+/K+-symport, maintain K+/Na+ homeostasis and increase salinity tolerance, can be classified into three subfamilies in higher plants. In this review, we systematically analyzed the characteristics of amino acids sequences and physiological functions of HKT transporters in higher plant. Furthermore, we depicted the hypothetical models of cations selection and transportation mediated by HKT transporters according to the highly conserved structure for the goal of better understanding the cations transportation processes. © 2015 Su et al.; licensee BioMed Central.


Iser M.,Hunan Agricultural University | Iser M.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Iser M.,CAS Institute of Subtropical Agriculture | Iser M.,University of Granma | And 13 more authors.
BioMed Research International | Year: 2016

This study was conducted to determine the effects of Agave fourcroydes powder as a dietary supplement on the growth performance, gut morphology, serum concentration of IgG, and the hematology parameters of broiler rabbits. A total of 32 rabbits [New Zealand × Californian] were weaned at 35 days. They were randomly selected for two dietary treatments (eight repetitions per treatment), which consisted of a basal diet and a basal diet supplemented with 1.5% dried-stem powder of A. fourcroydes. On day 60 from the initiation of treatment, gut histomorphology (duodenum and cecum), serum concentration of IgG, and hematology parameters were all measured. The results showed that A. fourcroydes powder supplementation improved (P < 0.05) the ADFI, ADG, and final BW. Correspondingly, this treatment increased (P < 0.05) the muscle and mucosa thickness and height and width of villi. However, duodenum crypts depth was lower (P < 0.05) when rabbits were fed with this natural product, compared with the basal diet treatment. Results also indicated that the A. fourcroydes powder increased (P < 0.05) the serum concentration of IgG but did not change the hematology parameters. This data indicates that A. fourcroydes powder, as a supplement, had beneficial effects on increasing the growth performance and serum concentration of IgG, as well as improving the gut morphology without affecting the hematology parameters in broiler rabbits. © 2016 Maidelys Iser et al.


Liu X.,Hunan Agricultural University | Liu X.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | Zheng S.,Hunan Agricultural University | Zheng S.,Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients | And 8 more authors.
Food Analytical Methods | Year: 2016

In this paper, a low-cost, simple, and highly sensitive electrochemical immunosensor was fabricated for the tetracycline detection based on gold electrode-modified carboxyl-Fe3O4 nanoparticle (MNPs) by chitosan (CS) as linker. The anti-tetracycline monoclonal antibody (Ab) was immobilized on the modified electrode surface. The binding of tetracycline to Ab was analyzed by differential pulse voltammetry. Here, MNPs were used as the signal amplifier to improve the sensitivity of the immunosensor. The stepwise assembly process of the electrochemical immunosensor was characterized by cyclic voltammetry and electrochemical impedance spectroscopy. Under the optimum operating conditions, the fabricated immunosensor showed a linear current response to the target concentration in the range from 0.08 to 1 ng/mL with a lower detection limit of 0.0321 ng/mL (S/N = 3). It was successfully applied to the detection of tetracycline in milk. Enzyme-linked immunoassay analysis was also conducted to detect tetracycline in the same samples for demonstrating the applicability of the electrochemical immunosensor. © 2016 Springer Science+Business Media New York


Ran T.,CAS Institute of Subtropical Agriculture | Ran T.,University of Chinese Academy of Sciences | Li H.,CAS Institute of Subtropical Agriculture | Li H.,University of Chinese Academy of Sciences | And 15 more authors.
Livestock Science | Year: 2016

In non-ruminants, sweet taste receptor and monosaccharide transporters are important components of nutrient chemosensing in the intestinal tracts. Ruminants, however, have evolved a polygastric digestive system linked to a transition in nutrient supply from readily-digestible carbohydrate (lactose) in milk during the pre-ruminant suckling phase to poorly-digestible structural carbohydrates when the rumen becomes functional. Whether these developmental and feed changes alter the mRNA expression of genes related to monosaccharide sensing (Taste receptor family 1 member 2, T1R2; Taste receptor family 1 member 3, T1R3) and transporting (monosaccharide transporters Na+/glucose co-transporter, SGLT1; glucose activated ion channel, SGLT3; solute carrier family 2 member 5, GLUT5; solute carrier family 2 member 2, GLUT2) are unknown. This study investigates the expression of sweet taste receptors (T1R2 and T1R3) and monosaccharides transporters (SGLT1, SGLT3, GLUT5 and GLUT2) along the gastrointestinal tracts (GIT) during different stages of development (suckling, weaning and grazing) in goats. The results showed that the expression of T1R2 decreased with age, and was mainly expressed in the duodenum and jejunum. Although T1R3 expression in different GIT segments fluctuated during growth, there was an increase with age, with the abomasum showing the greatest expression. Both SGLT1 and SGLT3 were mainly expressed in the jejunum at all ages, with the greatest expression in the middle jejunum during suckling, and decreased greatly (P<0.05) during weaning and grazing. GLUT5 was mainly expressed at the duodenum and jejunum, with differences (P<0.05) between suckling and the later developmental stages. No expression of GLUT5 was detected at the rumen and abomasum at any stage, but was noted in the ileum, cecum, colon and rectum during suckling, but with lower abundances during weaning and grazing. The expression of GLUT2 was detected only in the small intestine and decreased with age. The expression of T1R2 correlated (P<0.05) with SGLT1, SGLT3, GLUT5 and GLUT2. There were correlations (P<0.01) between the expressions of SGLT3 and SGLT1, as well as between SGLT3 and GLUT5. The current results indicate that: (1) the ability of the GIT of goats to absorb monosaccharides varies during development, being greatest at the suckling stage; (2) the duodenum and jejunum play a critical role in sensing and absorption of monosaccharides; (3) the mRNA expressions of sweet taste receptors and various transporters correlated well in the GIT of goats. © 2016 Elsevier B.V.


RAN T.,CAS Institute of Subtropical Agriculture | RAN T.,University of Chinese Academy of Sciences | ZHOU C.-S.,CAS Institute of Subtropical Agriculture | XU L.-W.,CAS Institute of Subtropical Agriculture | And 7 more authors.
Journal of Integrative Agriculture | Year: 2016

Quorum sensing (QS) is a type of microbe-microbe communication system that is widespread among the microbial world, particularly among microorganisms that are symbiotic with plants and animals. Thereby, the cell-cell signalling is likely to occur in an anaerobic rumen environment, which is a complex microbial ecosystem. In this study, using six ruminally fistulated Liuyang black goats as experimental animals, we aimed to detect the activity of quorum sensing autoinducers (AI) both in vivo and in vitro and to clone the luxS gene that encoded autoinducer-2 (AI-2) synthase of microbial samples that were collected from the rumen of goats. Neutral detergent fiber (NDF) and soluble starch were the two types of substrates that were used for in vitro fermentation. The fermented fluid samples were collected at 0, 2, 4, 6, 8, 12, 24, 36, and 48 h of incubation. The acyl-homoserine lactones (AHLs) activity was determined using gas chromatography-mass spectrometer (GC-MS) analysis. However, none of the rumen fluid extracts that were collected from the goat rumen showed the same or similar fragmentation pattern to AHLs standards. Meanwhile, the AI-2 activity, assayed using a Vibrio harveyi BB170 bioassay, was negative in all samples that were collected from the goat rumen and from in vitro fermentation fluids. Our results indicated that the activities of AHLs and AI-2 were not detected in the ruminal contents from six goats and in ruminal fluids obtained from in vitro fermentation at different sampling time-points. However, the homologues of luxS in Prevotella ruminicola were cloned from in vivo and in vitro ruminal fluids. We concluded that AHLs and AI-2 could not be detected in in vivo and in vitro ruminal fluids of goats using the current detection techniques under current dietary conditions. However, the microbes that inhabited the goat rumen had the potential ability to secrete AI-2 signaling molecules and to communicate with each other via AI-2-mediated QS because of the presence of luxS. © 2016 Chinese Academy of Agricultural Sciences

Loading Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients collaborators
Loading Hunan Co Innovation Center for Utilization of Botanical Functional Ingredients collaborators