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Lu L.,Central South University | Shan Y.,Central South University | Shan Y.,State and Local Joint Engineering Laboratory of Citrus Comprehensive Utilization | Shan Y.,Hunan Agricultural Product Processing Institute | Xia J.,Central South University
Journal of Chinese Institute of Food Science and Technology | Year: 2015

Takeing naringin as inducer, a strain with the ability producing naringinase was obtained from the soil and rutten citrus peel by dilution plate method and transparent circle screening. The naringinase producing capability reached 264.07 U/mL. By observation of morphological and phylogenetic tree analysis which based on rDNA-ITS sequence alignment, the strain was identified as Aspergillus aculeatus. The strain has strong degradation of naringin ability and also has potential to be exploited in strain breeding. ©, 2015, Chinese Institute of Food Science and Technology. All right reserved.


Li P.-J.,Central South University | Xia J.-L.,Central South University | Shan Y.,Hunan Academy of Agricultural science | Shan Y.,Hunan Agricultural Product Processing Institute | And 7 more authors.
Waste and Biomass Valorization | Year: 2014

A novel strain Penicillium oxalicum PJ02 was isolated for the production of exo-pectinase, endo-pectinase, carboxymethylcellulase (CMCase) and xylanase. Response surface methodology (RSM) was employed to optimize orange peel submerged fermentation for pectinase production. Plackett–Burman factorial design was used to select significant parameters. Temperature and NH4Cl concentration had significant influence on exo-pectinase production. The central composite design was used to generate a total of 22 fermentation experiments. The statistical results indicated that the optimum temperature for exo-pectinase and endo-pectinase production was 36.5 °C, and optimal NH4Cl concentration was 1.12 g/L. Under this condition, the yield of exo-pectinase was 36.88 U/mL, endo-pectinase was 0.62 U/mL, which are in close agreement with the values predicted by the model. The CMCase and xylanase yield in the same medium were 0.38 and 0.57 U/mL, respectively. RSM was also used to analyse the effect of independent variables on CMCase and xylanase production. © 2014, Springer Science+Business Media Dordrecht.


He D.,Hunan Normal University | He D.,University of South China | Shan Y.,Hunan Agricultural Product Processing Institute | Wu Y.,Hunan Agricultural Product Processing Institute | And 3 more authors.
Food Chemistry | Year: 2011

A simple and accurate method has been developed to simultaneously separate and determine 10 bioactive compounds in citrus fruits by high-performance liquid chromatography coupled with diode array detection and electrospray ionisation mass spectrometry (HPLC-DAD-ESI/MS). This HPLC assay was performed on a reversed-phase C18 column with acetonitrile and 0.1% (v/v) aqueous formic acid as mobile phase. DAD has been performed at 273, 283 and 324 nm for quantification of the alkaloids, flavanones and hydroxycinnamic acids. MS was also employed to identify the each analyte. Ten analytes (naringin, hesperidin, neohesperidin, ferulic acid, p-coumaric acid, chlorogenic acid, caffeic acid, octopamine, synephrine and tyramine) demonstrated good linearity (r ≥ 0.9990) in a relatively wide concentration range. The method revealed high average recovery (range, 92.1-97.9%) and good precision with interday and intraday variations with less than 4.71%. The limits of detection (LODs) ranged from 0.02 to 0.11 μg/ml, while the limits of quantification (LOQs) were defined in the range of 0.08-0.39 μg/ml. The proposed method has been successfully applied to analyse three types of bioactive constituents in eight citrus hybrids pulps and eight citrus hybrids peels, which has been successfully cultivated in China. © 2011 Elsevier Ltd. All rights reserved.


PubMed | State Key Laboratory of SubHealth Intervention Technology, Hunan Agricultural University and Hunan Agricultural Product Processing Institute
Type: Journal Article | Journal: Molecular medicine reports | Year: 2015

Ganoderma lucidum polysaccharides (GLPs), which were purified from the medicinal herb G. lucidum followed by ethanol precipitation, protein depletion using the Sevage assay, purification using DEAEcellulose (DE-52), dialysis and the use of ultrafiltration membranes, are used as an ingredient in traditional anticancer treatments in China. The aim of the current study was to evaluate the anticancer effects and investigate the underlying molecular mechanisms of GLPs on LoVo human colon cancer cells. The results demonstrated that the GLPmediated anticancer effect in LoVo cells was characterized by cytotoxicity, migration inhibition, enhanced DNA fragmentation, morphological alterations and increased lactate dehydrogenase release. Furthermore, the activation of caspases3, 8 and 9 was involved in GLPstimulated apoptosis. Additionally, treatment with GLPs promoted the expression of Fas and caspase3 proteins, whilst reducing the expression of cleaved poly(ADPribose) polymerase. These data indicate that GLPs demonstrate potential antitumor activity in human colon cancer cells, predominantly through the inhibition of migration and induction of apoptosis. Furthermore, activation of the Fas/caspase-dependent apoptosis pathway is involved in the cytotoxicity of GLPs.


Zhou G.,Hunan Agricultural University | Yang H.,Hunan Agricultural University | Zhou H.,Hunan Agricultural University | Zhou H.,Hunan Co Innovation Center for Ultilization of Botanical Functional Ingredients | And 8 more authors.
Journal of Biotechnology | Year: 2016

Streptomyces sp. Strain CdTB01, which is tolerant to high concentrations of heavy metals, particularly cadmium, was isolated from soil contaminated with heavy metals. Two contigs with total genome size of 10.19 Mb were identified in the whole genome sequencing and assembly, and numerous homologous genes known to be involved in heavy metal resistance were found in the genome. © 2016 Elsevier B.V.


Liang Z.-E.,Hunan Agricultural Product Processing Institute | Liang Z.-E.,Hunan Agricultural University | Yi Y.-J.,Hunan Agricultural University | Guo Y.-T.,State Key Laboratory of Sub Health Intervention Technology | And 3 more authors.
Molecular Medicine Reports | Year: 2015

Ganoderma lucidum polysaccharides (GLPs), which were purified from the medicinal herb G. lucidum followed by ethanol precipitation, protein depletion using the Sevage assay, purification using DEAE-cellulose (DE-52), dialysis and the use of ultrafiltration membranes, are used as an ingredient in traditional anticancer treatments in China. The aim of the current study was to evaluate the anticancer effects and investigate the underlying molecular mechanisms of GLPs on LoVo human colon cancer cells. The results demonstrated that the GLP-mediated anticancer effect in LoVo cells was characterized by cytotoxicity, migration inhibition, enhanced DNA fragmentation, morphological alterations and increased lactate dehydrogenase release. Furthermore, the activation of caspases-3, -8 and -9 was involved in GLP-stimulated apoptosis. Additionally, treatment with GLPs promoted the expression of Fas and caspase-3 proteins, whilst reducing the expression of cleaved poly(ADP-ribose) polymerase. These data indicate that GLPs demonstrate potential antitumor activity in human colon cancer cells, predominantly through the inhibition of migration and induction of apoptosis. Furthermore, activation of the Fas/caspase-dependent apoptosis pathway is involved in the cytotoxicity of GLPs.


PubMed | Hunan Agricultural University and Hunan Agricultural Product Processing Institute
Type: | Journal: Journal of biotechnology | Year: 2016

Streptomyces sp. Strain CdTB01, which is tolerant to high concentrations of heavy metals, particularly cadmium, was isolated from soil contaminated with heavy metals. Two contigs with total genome size of 10.19Mb were identified in the whole genome sequencing and assembly, and numerous homologous genes known to be involved in heavy metal resistance were found in the genome.


Zhang C.,Central South University | Shan Y.,Central South University | Shan Y.,Citrus Resources Comprehensive Utilization of Local and National Joint Project Laboratory | Shan Y.,Hunan Agricultural Product Processing Institute
Journal of Chinese Institute of Food Science and Technology | Year: 2013

Acid extraction, ion-exchange resin, ammonium oxalate extraction and enzyme extraction are four main technologies used in citrus peel pectin extraction. Microware extraction has replaced the traditional extraction methods as a new technology gradually. The pectin industry in national country will be promoted with the emerging of microwave independent extraction. Compared with the microwave-assisted extraction technology, microwave independent extraction catches much attention because of its green, efficient, convenient, fast and high yield of output. It is a pectin extraction method in industry. The methods of traditional pectin extraction, microwave-assisted extraction in national country and microwave independent extraction in abroad were summarized. The traditional and the microwave methods were compared. In addition, the current development situation and prospect of citrus peel pectin extraction were also discussed.


Li L.,Research Team of Crop Starch Chemistry and Metabonomics Hunan Academy of Agricultural science | Li L.,Central South University | Li L.,Hunan Agricultural Product Processing Institute | Tang H.,Research Team of Crop Starch Chemistry and Metabonomics Hunan Academy of Agricultural science | And 3 more authors.
Journal of the Chinese Cereals and Oils Association | Year: 2015

In order to evaluate nutritional quality of BJBH (edible lily) from Hunan Province, the major nutrients, texture properties of fresh and cooked lily, gel texture and swelling power of flour and starch have been studied in the experiment as well as starch physicochemical properties. The major nutrients including protein, starch and DF showed significant differences compared with existing research achievements. The variation of texture properties after prepared was mainly caused by gelatinization of the macromolecular substances including starch and DF, etc. The values of swelling power for flour and starch was 6.2 g/g and 14.5 g/g respectively. Difference of gel texture between flour and starch occurred by cellulose and other insoluble polysaccharide. The sizes granules ranged from 9.09 to 34.57 μm and the median size was 19.28 μm. The crystal structure was type B and the relative crystallinity was 54.4%. T0, TP, TC and ΔHgel were 54.0, 63.2, 75.0℃ and 13.3 J/g respectively. Enzyme hydrolysis rate of starch granules was first quick then slowed down, and then it kept stability after 72 h. The water absorption of starch granules was first slow and then fast, and the maximum water absorption was 215 mg/g at humidity of 100%. © 2015, Editorial Department, Chinese Cereals and Oils Association. All right reserved.

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