Hunan Agricultural Bioengineering Research Institute

Changsha, China

Hunan Agricultural Bioengineering Research Institute

Changsha, China
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Xiao H.-B.,Hunan Agricultural University | Fang J.,Hunan Agricultural University | Lu X.-Y.,Hunan Agricultural Bioengineering Research Institute | Sun Z.-L.,Hunan Veterinary Drug Engineering Technology Center
British Poultry Science | Year: 2012

1. The objective of this study was to examine the beneficial effects of kaempferol, a naturally occurring polyphenol, on carcase characteristics in broiler chickens and the mechanisms involved in this regulation.2. Broiler chickens were randomly divided into 7 groups: control, carrier control, kaempferol (0·3%), kaempferol (0·6%), hypercholesterolemic (HLD), HLD and kaempferol (0·3%), HLD and kaempferol (0·6%).3. Seven weeks after treatment, carcase characteristics, lipid levels in the blood and liver, expression of hepatic Angiopoietin-like protein 3 (ANGPTL3) mRNA, and expression of adipose lipoprotein lipase (LPL) protein were determined.4. Treatment with kaempferol (0·3 or 0·6%) significantly increased plasma high-density lipoprotein cholesterol levels, decreased percentage of abdominal fat, thickness of subcutaneous fat, plasma and hepatic total cholesterol and triglyceride concentrations, muscle malondialdehyde level and down-regulated expression of ANGPTL3 mRNA concomitantly with up-regulated expression of LPL protein in normal and hypercholesterolemic broiler chickens.5. Kaempferol (0·3 or 0·6%) treatment had no significant effect on the values of percentage of breast muscle, percentage of leg muscle, carcase weight and eviscerated percentage.6. The results suggest that kaempferol improves carcase characteristics by decreasing expression of ANGPTL3 in broiler chickens. © 2012 Copyright Taylor and Francis Group, LLC.


Wei B.,Hunan Agricultural University | Gao G.,Hunan Academy of Agricultural science | Tian Y.,Hunan Agricultural University | Tian Y.,Hunan Agricultural Bioengineering Research Institute | And 5 more authors.
Huanjing Kexue Xuebao/Acta Scientiae Circumstantiae | Year: 2011

Aspergillus niger isolated from soil by enrichment culture was chosen as the research object in this paper. The decoloring properties of Aspergillus niger to rose bengal and water-soluble color paste were studied in liquid culture with different carbon and nitrogen sources, pH value, and added metal ions. The decoloring effect was the best in a medium of bean sauce, with glycerol as carbon source, (NH4)2SO4 for nitrogen source, and initial pH of 6.0~8.0, but Cu2+ obviously inhibited the activity. By comparing the decoloring ability of living mycelium and dead mycelium, combined with correlation analysis results of mycelium dry weight and decoloring ability, we initially speculated that this fungus' decoloring mechanism was as follows: the hyphae adsorb the dyes, then the adsorbed dye is degraded by a rate-limiting enzymatic process. Sodium citrate, and sodium tartrate inhibited the enzyme activity and NH4 + activated its function.


Tian Y.,Hunan Agricultural University | Tian Y.,Hunan Agricultural Bioengineering Research Institute | Tian Y.,HIGH-TECH | Yang H.,Hunan Agricultural University | And 9 more authors.
Plant Growth Regulation | Year: 2010

Stamen is the male reproductive organ of rice (Oryza sativa L.), and the development is a considerably significant stage during the sexual reproduction. It is very important to reveal what is the molecular mechanisms which controlling the development of stamen, and will be helpful in producing hybrid seeds by manipulating the male sterility. This article reviews the progress on the molecular mechanisms of male reproductive organogenesis in rice, which would facilitate the further studies on male sterile genes in rice. © Springer Science+Business Media B.V. 2010.


Xiao G.-Q.,Hunan Agricultural University | Xiao G.-Q.,Hunan Agricultural Bioengineering Research Institute | Zhang H.-W.,Chinese Academy of Agricultural Sciences | Lu X.-Y.,Hunan Agricultural University | And 2 more authors.
Journal of Integrative Agriculture | Year: 2015

A novel rice lesion mimic mutant (LMM) was isolated from an ethane methyl sulfonate (EMS)-induced 02428 mutant bank. The mutant, tentatively designated as lmm6, develops necrotic lesions in the whole growth period along with changes in several important agronomic traits. We found that the initiation of the lesions was induced by light and cell death occurred in lmm6 accompanied with accumulation of reactive oxygen species (ROS). The lower chlorophyll content, soluble protein content and superoxide dismutase (SOD) activity, the higher malondialdehyde (MDA) content were detected in lmm6 than in the wild type (WT). Moreover, the observation by transmission electronic microscope (TEM) demonstrated that some organelles were damaged and the stroma lamella of chloroplast was irregular and loose in mesophyll cell of lmm6. In addition, lmm6 was more resistant than WT to rice blast fungus Magnaporthe grisea infection, which was consistent with increased expression of four genes involved in the defense-related reaction. Genetic analysis showed that mutant trait of lmm6 is inherited as a monogenic recessive nuclear gene located on the long arm of chromosome 6. Using simple sequence repeat (SSR) markers, the target gene was finally delimited to an interval of 80.8 kb between markers MM2359 and MM2370, containing 7 annotated genes. Taken together, our results provide the information to identify a new gene involved in rice lesion mimic, which will be helpful in clarifying the mechanism of cell death and disease resistance in rice. © 2015 Chinese Academy of Agricultural Sciences.


Tian Y.,Hunan Agricultural University | Tian Y.,Hunan Agricultural Bioengineering Research Institute | Tian Y.,HIGH-TECH | Zhang C.,Hunan Agricultural University | And 10 more authors.
Russian Journal of Plant Physiology | Year: 2011

The full-length cDNA encoding a TRANSPORT INHIBITOR RESPONSE 1 (TIR1) protein, designated NtTIR1, was isolated for the first time from Nicotiana tabacum by the rapid amplification of cDNA ends (RACE) method. NtTIR1 contained a 1746-bp open reading frame encoding 581 amino acids. The deduced NtTIR1 protein, which showed high identity to TIR1 protein of other dicotyledonous plants, had a calculated mol wt of 65.2 kD and a theoretical pI value of 6.02 and was predicted to possess an F-box domain. Bioinformatic analyses revealed that the deduced NtTIR1 contained three transmembrane domains, and the predicted 3D model of NtTIR1 had a typical spatial structure of the TIR1 protein from Arabidopsis thaliana. Transcription pattern analysis revealed that the transcription of NtTIR1 was induced by IAA and ABA. Cloning of the NtTIR1 gene will enable us to further understand the molecular organization of the TIR1 and its possible function in the tobacco. © 2011 Pleiades Publishing, Ltd.


Tian Y.,Chinese Academy of Agricultural Sciences | Tian Y.,Hunan Agricultural University | Tian Y.,Hunan Agricultural Bioengineering Research Institute | Zhang H.,Chinese Academy of Agricultural Sciences | And 6 more authors.
Transgenic Research | Year: 2011

Rice (Oryza sativa L.) is a warm-season plant exposed to various stresses. Low temperature is an important factor limiting extension of rice cultivation areas and productivity. Previously, we have demonstrated that tomato ERF protein TERF2 enhances freezing tolerance of transgenic tobacco and tomato plants. Herein, we report that overexpression of TERF2 enhances transgenic rice tolerance to cold without affecting growth or agronomic traits. Physiological assays revealed that TERF2 could not only increase accumulation of osmotic substances and chlorophyll, but also reduce reactive oxygen species (ROS) and malondialdehyde (MDA) content and decrease electrolyte leakage in rice under cold stress. Further analysis of gene expression showed that TERF2 could activate expression of cold-related genes, including OsMyb, OsICE1, OsCDPK7, OsSODB, OsFer1, OsTrx23, and OsLti6, in transgenic rice plants under natural condition or cold stress. Thus, our findings demonstrated that TERF2 modulated expression of stress-related genes and a series of physiological adjustments under cold stress, indicating that TERF2 might have important regulatory roles in response to abiotic stress in rice and possess potential utility in improving crop cold tolerance. © 2010 Springer Science+Business Media B.V.


Jin Y.,Tobacco Science Research Institute of Yunnan Province | Zhang C.,Hunan Agricultural University | Zhang C.,Hunan Agricultural Bioengineering Research Institute | Yang H.,Hunan Agricultural University | And 7 more authors.
African Journal of Biotechnology | Year: 2011

Cold stress is one of the major abiotic stresses limiting the productivity and the geographical distribution of many important crops. To gain a better understanding of cold stress responses in tobacco (Nicotiana tabacum), we carried out a comparative proteomic analysis. Five-week-old tobacco seedlings were treated at 4°C for 4 h. Cold treatment resulted in stress phenotypes of smoothing and shallowing leaves and increased relative electrolyte leakage. The expression changes of total proteins in tobacco leaves were examined using two-dimensional electrophoresis. Quantitative image analysis revealed a total of 101 protein spots that changed their intensities significantly, 21 protein spots were down-regulated, eight were up-regulated after the cold treatment, 50 protein spots only expressed in the control sample, while 22 protein spots were only present in the cold treatment sample. Mass spectrometry analysis allowed the identification of 73 differentially expressed proteins, including well known and novel cold-responsive proteins. The identified proteins are involved in several processes such as photosynthesis, protein processing, redox homeostasis, ribonucleic acid (RNA) processing, signal transduction, translation, cell division/cycle, and metabolisms of carbon and energy. Several types of proteins showed enhanced degradation during chilling stress, especially the photosynthetic proteins. Gene expression analysis of 25 different proteins by reverse transcriptase-polymerase chain reaction (RT-PCR) showed that the messenger RNA (mRNA) levels of 18 genes correlated well with the protein levels. In conclusion, our study provides new insights into cold stress responses in tobacco and needs to be further studied in future. © 2011 Academic Journals.


Dai Q.,Hunan Agricultural University | Dai Q.,HIGH-TECH | Tian Y.,Hunan Agricultural University | Tian Y.,HIGH-TECH | And 3 more authors.
African Journal of Biotechnology | Year: 2012

Rice (Oryza sativa) is an aerobic organism, for which oxygen shortage poses a serious problem. Longtime complete submergence leads to damage or even death of the plant. Submergence-tolerant rice adapts to flood (complete submergence) in a long time. Ethylene regulation networks and sugar signaling are involved in the submergence response of rice. This is mainly through the regulation of many key genes such as SUB1A and SK1/2; therefore, the level of abscisic acid (ABA) and gibberellic acid (GA) was regulated. This paper reviews these two signaling pathways, understanding which can help us develop new varieties and improve existing cultivars. © 2012 Academic Journals.


Liu H.,Hunan Agricultural University | Liu H.,Hunan Agricultural Bioengineering Research Institute | Tian Y.,Hunan Agricultural University | Tian Y.,Hunan Agricultural Bioengineering Research Institute | And 3 more authors.
Energy Sources, Part A: Recovery, Utilization and Environmental Effects | Year: 2015

Developing the biomass energy industry has become a new idea for solving the energy crisis. The plant, Miscanthus, which has a high content of cellulose, is an optimal material for biorefinery. In this review, the characteristics of Miscanthus are first briefly introduced. Then, the biorefinery general scheme, main process, and existing problems of Miscanthus are summarized. Finally, its application is prospected. © Copyright © Taylor & Francis Group, LLC.


Xiao H.-B.,Hunan Agricultural University | Lu X.-Y.,Hunan Agricultural Bioengineering Research Institute | Zhang H.-B.,Red Cross | Sun Z.-L.,Hunan Agricultural University | Fang J.,Hunan Agricultural University
Journal of Physiology and Biochemistry | Year: 2013

Angiopoietin-like protein 3 (Angptl3)-lipoprotein lipase (LPL) pathway may be a useful pharmacologic target for hyperlipidemia. The present study was conducted to test the effect of soluble fiber extracted from Undaria pinnatifida (UP), on hyperlipidemia in apolipoprotein E-deficient (ApoE-/-) mice. Forty mice were divided into four groups (n = 10): control group (C57BL/6J mice), ApoE-/- mice group, and two groups of ApoE-/- mice treated with UP fiber (5 or 10 % per day). UP soluble fiber treatment significantly decreased plasma and hepatic total cholesterol, triglycerides levels, plasma low-density lipoprotein cholesterol, and malondialdehyde concentrations and increased plasma high-density lipoprotein cholesterol level and downregulated protein expression of Angptl3 concomitantly with upregulated protein expression of LPL. In addition, T0901317 caused elevated expression of hepatic Angptl3 protein, and the effect of T0901317 was also abrogated by UP soluble fiber in C57BL/6J mice. The present results suggest that the UP soluble fiber regulates Angptl3-LPL pathway to lessen hyperlipidemia in mice. © 2013 University of Navarra.

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