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Russian Federation, Russia

Bozo I.Y.,Moscow State University | Zorin V.L.,Human Stem Cells Institute | Eremin I.I.,Ai Burnasyan Federal Medical Biophysical Center | Deev R.V.,Kazan Federal University | And 3 more authors.
Genes and Cells | Year: 2014

Multipotent mesenchymal stromal cells (MMSC) derived from various intraoral sources attracts attention of an increasing number of researchers due to availability and some features making them different from MMSC of bone marrow or adipose tissue. The review describes the main intraoral sources, characterizes their anatomical, topographical and histological peculiarities which may influence on MMSC morphofunctional profile. In comparative aspect we systematized the principal data concerning properties of MMSC derived from gingiva, buccal mucosa; periodontal ligament, dental pulp, apical papilla, jaw periosteum. It is not possible to compare MMSC of various intraoral sources under some parameters because of absence appropriate experimental findings. Some additional difficulties are caused by contradictions in published materials that are discussed in the review as well.

Ryabov A.Y.,Dentisrty Center Interdentos | Fadeeva I.S.,Russian Academy of Sciences | Deev R.V.,Human Stem Cells Institute | Vezhnina N.O.,Russian Academy of Sciences | And 6 more authors.
Genes and Cells | Year: 2014

In the last decade barrier the application of different types of membranes in the oral and maxillofacial surgery has been increasing. Their main reliability features include resorption times, the lack of toxic and antigenic effects, the absence of negative effects from the membrane on the structure of the surrounding soft tissues, as well as a high degree of biointegration and a potentiality of steady stabilization in the wound. Considering the abovementioned parameters, graft barrier membranes are a material of choice in the modern reconstructive oral and maxillofacial surgery. The present study includes an experimental and morphological assessment of biologic xenogenic barrier membranes for the evaluation of their immunological potency and the time of biodegradation using a model test system in vivo. Three groups of barrier membranes were assayed 'M-D (decellularized diaphragm), M-P (pericardium), M-DM (dura mater), prepared from the corresponding tissues of a mature ox, in accordance to the method developed in the Priorov Central Research Institute of Traumatology and Orthopaedics. The experiments were carried out on a model of 6-week heterotopic subcutaneous implantation in rats and included a complex histological and immunohistochemical analysis, with quantitative estimation of biograft mineralization by adsorption spectroscopy. It was found that: the material of M-DM has the highest and fullest (while retaining the strength properties) resorption; a relatively long bi-odegradation time, expressed conductive effect and constraint function are specific for the Ì-P material; M-D membranes have faster resorption, enhanced thinning and relative biological inertness. The results of the conducted study indicate that all the implanted materials are biocompatible and efficient, and thus may be used in various medical cases.

Deev R.V.,Kazan Federal University | Drobyshev A.Y.,Moscow State University | Bozo I.Y.,Moscow State University | Isaev A.A.,Human Stem Cells Institute
BioMed Research International | Year: 2015

Bone grafts are medical devices that are in high demand in clinical practice for substitution of bone defects and recovery of atrophic bone regions. Based on the analysis of the modern groups of bone grafts, the particularities of their composition, the mechanisms of their biological effects, and their therapeutic indications, applicable classification was proposed that separates the bone substitutes into "ordinary" and "activated." The main differential criterion is the presence of biologically active components in the material that are standardized by qualitative and quantitative parameters: growth factors, cells, or gene constructions encoding growth factors. The pronounced osteoinductive and (or) osteogenic properties of activated osteoplastic materials allow drawing upon their efficacy in the substitution of large bone defects. © 2015 R. V. Deev et al.

Solovyeva V.V.,Kazan Federal University | Isaev A.A.,Human Stem Cells Institute | Genkin D.D.,OJSC Pharmsynthez | Rizvanov A.A.,Kazan Federal University
Cellular Transplantation and Tissue Engineering | Year: 2012

Lentiviral vectors are widely used in genetic modification of human and animal cells (lentiviral transduction) to enhance their therapeutic potential by expression of recombinant protective and trophic factors. Genetic modification of cells in vitro or ex vivo achieves the specificity of viral transduction, as modified are just cells that have been manipulated in the laboratory. In addition, the introduction of genetically modified cells, but not pure virus, helps to avoid introduction of viral particles into the body of the recipient. This approach allows us to control the expression of therapeutic genes, the immunogenicity of viral vectors and viral transduction. To date, different approaches are used to improve the lentiviral transduction (polycations, protamine sulfate, etc.), but these methods suffer from limited efficacy or high toxicity. For the first time we demonstrated that the recombinant histone N1.3 increases the efficiency of lentiviral transduction by more than 2 times and has no toxic effect on target cells in a wide range of concentrations studied.

Chogovadze A.G.,Human Stem Cells Institute
Cellular Transplantation and Tissue Engineering | Year: 2012

Advances in methods of molecular genetic diagnosis have made it possible to determine whether a child will be born with a certain genetic disorder. And the use of assisted reproductive technologies make it possible to screen for genetic conditions at an earlier embryonic stage, before implanting the embryo in the mothers uterus. However, such diagnostic technologies can potentially be used for non-medical purposes, i.e., for sex selection. This contradicts to modern concepts of bioethics, so most countries have introduced legislative restrictions on the conduction of such genetic testing.

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