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Yao X.,HubeiUniversityofTechnology | Meran S.,University of Cardiff | Fang Y.,HubeiUniversityofTechnology | Martin J.,University of Cardiff | And 7 more authors.
Food Hydrocolloids | Year: 2014

It has been suggested that the Traditional Chinese herbal preparation Cordyceps sinensis (CS) may have a beneficial effect in renal disease. We have previously demonstrated bio-activity of naturally occurring CS. To satisfy increasing demand, CS derivatives have been produced by aseptic mycelia cultivation. The aim of this work was to compare and contrast the bio-activity of natural CS and two specific products derived from cultured Cordyceps mycelia, "Jinshuibao Capsule" ( Paecilomyces sinensis) and "Corbrin Capsule" ( Hirsutella sinensis). In addition using two different cell systems we examined the potential anti-fibrotic potential of these products beyond the kidney.All three preparations antagonised the activity of the pro-fibrotic cytokine TGF-β1 in renal epithelial cells. Activity was seen in the raw water-soluble components, and the hydrophilic and hydrophobic extracts from each preparation suggesting that the bio-activity is not confined to one component of the raw material. In addition to the effects in the renal cell line, similar effects of antagonism of phenotypic activation of pulmonary fibroblasts by TGF-β1 was also seen suggesting an applicability of these observations to solid organ fibrosis beyond the kidney. Mechanistically the data demonstrate antagonism of TGF-β1 activation of Smad signalling and specifically of induction of the hyaladherin TSG-6, which we have previously demonstrated to orchestrate the formation of a hyaluronan, peri-cellular coats driving phenotypic cellular activation.In summary the data support the claim that both naturally occurring and products derived from cultured Cordyceps mycelia have similar bio-activity which may have particular relevance for solid organ fibrosis and associated organ dysfunction. © 2013.


Zhang L.,HubeiUniversityofTechnology | Shi Z.,HubeiUniversityofTechnology | Shangguan W.,HubeiUniversityofTechnology | Fang Y.,HubeiUniversityofTechnology | And 5 more authors.
Food Hydrocolloids | Year: 2015

Sugar beet pectin (SBP) was enzymatically modified by crosslinking ferulic acid groups using horseradish peroxidase (HRP). The purpose of the modification was to achieve maximum molecular weight while retaining reasonably good solubility of the SBP. Reaction conditions were optimized in terms of pH (5.0-7.0), H2O2 concentration (0.25-1.0mM) and HRP dose (0.0017-0.0083U/mL). A maximum molecular weight of 1.86×106Da was obtained at pH=6.5, H2O2=1.0mM and HRP=0.005U/mL while also remaining good solubility. The emulsifying properties of the SBPs before and after modification were evaluated. The emulsion prepared using the modified SBP and medium chain triglyceride (MCT) showed improved stability during acceleration test at 60°C for a period of 10 days, in comparison with the emulsion prepared with control SBP. The volume-weighted mean diameter D4, 3 after 10-day acceleration was 12.1 and 20.6μm for emulsions stabilized with modified and control SBPs, respectively. © 2014 Elsevier Ltd.

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