Hubei Zhongshan Hospital

Wuhan, China

Hubei Zhongshan Hospital

Wuhan, China
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Xing F.,Shanghai University of Traditional Chinese Medicine | Xing F.,Shanghai Key Laboratory of Traditional Chinese Clinical Medicine | Tan Y.,Shanghai University of Traditional Chinese Medicine | Tan Y.,Shanghai Key Laboratory of Traditional Chinese Clinical Medicine | And 7 more authors.
Journal of Ethnopharmacology | Year: 2012

Background: Conventional methods of treating cirrhotic ascites are inadequate. We sought to identify a novel, effective approach to relieve the suffering of patients with cirrhotic ascites. Aim of the study: To investigate the efficacy of Xiaozhang Tie, a traditional Chinese herbal cataplasm composed of dahuang (Rheum palmatum L.), laifuzi (Raphanus sativus L.), concocted gansui (Euphorbia kansui T.N. Liou ex T.P. Wang), chenxiang [Aquilaria sinensis (Lour.) Gilg], dingxiang (Eugenia caryophyllata Thunb.), bingpian (Borneolum syntheticum) and shexiang (artificial Moschus), as an adjuvant in treating cirrhotic ascites. Materials and methods: A multicenter, randomized, placebo-controlled trial was conducted. One hundred patients with cirrhotic ascites were divided into two groups of equal size. The test group took an umbilical compress with Xiaozhang Tie for 30 days while the control group was administered an umbilical compress with placebo, in addition to primary therapy. Efficacy was evaluated according to the criteria including ascites volume, urine 24-h volume, abdominal circumference, body weight, abdominal distention, appetite, flatus and defecation. Results: Ninety-two patients completed the study, 7 were withdrawn and 1 was excluded. The effective rate of grades I and II was 63.3% for the test group (n = 49) and 38.0% for the control one (n = 50). Both groups showed decreased body weight and abdominal circumference, increased urine volume and improved symptoms after treatment. However, the differences between pre-treatment and post-treatment in body weight, abdominal circumference and urine volume were 8.7 ± 5.8 kg, 12.4 ± 8.3 cm and 683 ± 644 ml respectively in the test group, noticeably higher than those in the control group, which were 5.3 ± 4.6 kg, 8.0 ± 6.5 cm and 372 ± 697 ml, respectively. The ranking orders of the symptoms of the test group were significantly lower than those of the control group after treatment. No severe adverse reactions were seen. Conclusion: Xiaozhang Tie as an adjuvant to primary therapy of cirrhotic ascites is safe and shows a remarkable efficacy on relieving abdominal distention. © 2011 Elsevier Ireland Ltd.


Li R.-F.,Hubei Zhongshan Hospital | Li R.-F.,University of Chicago | Feng Y.-Q.,Industry Weapon | Chen J.-H.,Hubei Zhongshan Hospital | And 3 more authors.
Experimental and Therapeutic Medicine | Year: 2015

Treatments for leukemia remain unsatisfactory. Conventional chemotherapy agents that aim to kill tumor cells may also damage normal cells and thus result in severe side‑effects. Naringenin, a natural polyphenolic compound with antioxidant effects, has been revealed to have significant antitumor effects with low toxicity in preliminary studies. Thus, it is considered as one of the most promising flavonoids in the treatment of leukemia. In the present study, the effects of naringenin on the K562 human leukemia cell line and the underlying mechanisms were explored in vitro. In addition, human peripheral blood polymorphonuclear leukocytes (PMNs) were used as a normal control in order to evaluate the effects of naringenin on normal granulocytes and in the mediation of Adriamycin (ADM)‑induced oxidative damage. The results revealed that K562 proliferation was significantly inhibited by naringenin in a time‑ and concentration‑dependent manner; however, minimal cytotoxic effects were observed in PMNs when naringenin was used at concentrations <400 μmol/l. Morphological changes indicative of apoptosis were observed in naringenin‑treated K562 cells. Flow cytometric analysis indicated that the K562 cells were arrested in the G0/G1 phase of the cell cycle with a significantly upregulated rate of apoptosis. Furthermore, in the naringenin‑treated K562 cells, the labeling index of proliferating cell nuclear antigen was observed to be increased by immunochemical staining, the mRNA and protein expression levels of p21/WAF1 were strongly upregulated in reverse transcription‑polymerase chain reaction and western blot analyses, whereas p53 gene expression was not significantly changed. In PMNs to which naringenin (50~80 μmol/l) was added 1 h subsequent to ADM, the cell damage induced by ADM was significantly reduced, coincident with reductions in the levels of reactive oxygen species (ROS) and malondialdehyde (MDA) and increases in the activity of superoxide dismutase and glutathione peroxidase. However, the cytotoxic effect of ADM in K562 cells was not significantly altered by naringenin, and the oxidative stress indices in K562 cells remained stable. In conclusion, the present study revealed the promising value of naringenin in leukemia treatment. Naringenin demonstrated a significant inhibitory effect on the growth of K562 cells but not on normal PMNs. Furthermore, naringenin protected PMNs from ADM‑induced oxidative damage at low concentrations. Cell cycle arrest and apoptosis‑inducing effects, achieved through p53‑independent p21/WAF1 upregulation, are likely to be the mechanism of the antileukemic effects of naringenin, and the protective effect against ADM chemotherapy‑induced damage in PMNs may be due to the antioxidant capability of this agent at low concentrations. © 2015, Spandidos Publications. All rights reserved.


PubMed | Industry Weapon, Hubei Zhongshan Hospital, University of Chicago and Hubei University
Type: Journal Article | Journal: Experimental and therapeutic medicine | Year: 2015

Treatments for leukemia remain unsatisfactory. Conventional chemotherapy agents that aim to kill tumor cells may also damage normal cells and thus result in severe side-effects. Naringenin, a natural polyphenolic compound with antioxidant effects, has been revealed to have significant antitumor effects with low toxicity in preliminary studies. Thus, it is considered as one of the most promising flavonoids in the treatment of leukemia. In the present study, the effects of naringenin on the K562 human leukemia cell line and the underlying mechanisms were explored


Weng Z.,Huazhong University of Science and Technology | Weng Z.,Nanchang University | Zhou X.,Hubei Zhongshan Hospital | Liu X.,Nanchang University | And 3 more authors.
Journal of Craniofacial Surgery | Year: 2013

Trigeminal neuralgia is the worst pain that human beings have ever experienced. Few researches have illustrated perioperative pain in patients with trigeminal neuralgia undergoing radiofrequency thermocoagulation (RFT) of the gasserian ganglion under local anesthesia. Because there are some undeniable drawbacks of using intravenous short-term anesthesia during the intervention repeatedly, some physicians keep patients awake throughout the puncture procedure, using local anesthesia. The purpose of this investigation was to examine perioperative pain in patients with trigeminal neuralgia undergoing RFT of the gasserian ganglion. Participants were 104 patients with classic trigeminal neuralgia. Worst pain intensity, mean pain intensity, quality of sleep, and analgesia satisfaction were evaluated for 24 hours before admission, 24 hours before operation, and 24 hours after operation. Intraoperative worst pain intensity was determined. Preoperative pain was serious, and preoperative sleep quality significantly and positively correlated with preoperative mean pain (r = 0.52; P = 0.00) and worst pain (r = 0.49; P = 0.00). Few patients (1.9%) responded to preoperative treatment, and the preoperative treatment obtained low analgesia satisfaction scores (3.9 [1.3]). Most patients experienced severe pain during cannulation under local anesthesia. No patients complained of pain during radiofrequency lesioning. The RFT of the gasserian ganglion alleviated pain obviously. Most patients (94.2%) responded to the operation, and the operation got high analgesia satisfaction scores (8.9 [0.7]). The results demonstrate that preoperative pain in patients with trigeminal neuralgia undergoing RFT of the gasserian ganglion is prevalent and undertreated and that intraoperative pain is severe under local anesthesia during cannulation. © 2013 by Mutaz B. Habal, MD.


Wang X.-W.,Hubei Zhongshan Hospital | Zhang Y.-L.,Wuhan Institute of Tuberculosis Prevention | Xiong Y.,Wuhan Union Hospital
Genetic Testing and Molecular Biomarkers | Year: 2015

Objectives: To investigate the relationship between ABCB1 single-nucleotide polymorphisms and the efficacy of salmeterol/fluticasone combination (SFC) inhalation therapy for stable chronic obstructive pulmonary disease (COPD) in a Chinese Han population. Methods: A total of 362 patients with stable COPD were recruited between July 2012 and March 2014. Based on the therapeutic effects of lung function improvement and COPD Assessment Test (CAT) scores, all patients were either placed into the effective group (n=138) or the ineffective group (n=224). Three common polymorphisms (rs1045642C>T, rs1128503C>T, and rs1202184A>G) in the ABCB1 gene were analyzed by polymerase chain reaction-restriction fragment length polymorphism in these patients. All data were analyzed by SPSS version 18.0 software. Results: The genotype and allele frequencies of the ABCB1 rs1045642C>T polymorphic locus were significantly different between the effective group and the ineffective group under the codominant, recessive, and allele models (all p<0.05). Haplotype analysis of ABCB1 indicated that CTA (rs1045642C-rs1128503T-rs1202184A) haplotype frequencies in the effective group were significantly lower than the ineffective group (p=0.022), but TCG (rs1045642T-rs1128503C-rs1202184G) haplotype frequencies in the effective group were significantly higher than the ineffective group (p=0.048). Logistic regression analysis showed that smoking history and rs1045642 CT+CC/TT may be correlated with the efficacy of SFC inhalation therapy in stable COPD patients. Conclusion: ABCB1 rs1045642C>T polymorphism and CTA/TCG haplotypes, as well as smoking history may influence the efficacy of SFC inhalation therapy in stable COPD patients in the Chinese Han population. © Mary Ann Liebert, Inc. 2015.


PubMed | Wuhan Union Hospital, Hubei Zhongshan Hospital and Wuhan Institute of Tuberculosis Prevention
Type: Journal Article | Journal: Genetic testing and molecular biomarkers | Year: 2015

To investigate the relationship between ABCB1 single-nucleotide polymorphisms and the efficacy of salmeterol/fluticasone combination (SFC) inhalation therapy for stable chronic obstructive pulmonary disease (COPD) in a Chinese Han population.A total of 362 patients with stable COPD were recruited between July 2012 and March 2014. Based on the therapeutic effects of lung function improvement and COPD Assessment Test (CAT) scores, all patients were either placed into the effective group (n=138) or the ineffective group (n=224). Three common polymorphisms (rs1045642C>T, rs1128503C>T, and rs1202184A>G) in the ABCB1 gene were analyzed by polymerase chain reaction-restriction fragment length polymorphism in these patients. All data were analyzed by SPSS version 18.0 software.The genotype and allele frequencies of the ABCB1 rs1045642C>T polymorphic locus were significantly different between the effective group and the ineffective group under the codominant, recessive, and allele models (all p<0.05). Haplotype analysis of ABCB1 indicated that CTA (rs1045642C-rs1128503T-rs1202184A) haplotype frequencies in the effective group were significantly lower than the ineffective group (p=0.022), but TCG (rs1045642T-rs1128503C-rs1202184G) haplotype frequencies in the effective group were significantly higher than the ineffective group (p=0.048). Logistic regression analysis showed that smoking history and rs1045642 CT+CC/TT may be correlated with the efficacy of SFC inhalation therapy in stable COPD patients.ABCB1 rs1045642C>T polymorphism and CTA/TCG haplotypes, as well as smoking history may influence the efficacy of SFC inhalation therapy in stable COPD patients in the Chinese Han population.


Gong W.,Hubei Zhongshan Hospital
Medical Journal of Wuhan University | Year: 2011

Objective: To investigate the relationship between homocysteine (Hcy) and carotid intimamedia thickness (CIMT). Methods: Two hundred patients were selected. High-resolution ultrasound was used to scan carotid and brachial arteries of all patients in order to check and measure CIMT, plaques of carotid arteries, and diameter of brachial arteries at rest. Patients were divided into four groups; normal carotid intima group, diffused proliferative carotid intima group, stable plaque group and unstable plaque group. Serum levels of Hcy were determined with immunological method. The relationship between the results measured by ultrasound instrument and the concentrations of Hcy was analyzed. Results; The serum levels of Hcy in proliferative carotid intima group were higher than in the normal carotid intima group (P < 0.05). The serum levels of Hcy in unstable plaque group and in stable plaque group were higher than those of diffused proliferative carotid intima group (P < 0.05). The serum levels Hcy in the unstable plaque group were higher than in stable plaque group (P < 0.05). Conclusion; The increased concentrations of hcy were closely correlated with the increment of carotid intima-media thickness and the unsteady state of plaque.


Liu S.,Hubei Zhongshan Hospital | Fang Y.,Jiangsu University | Shen H.,Jiangsu University | Xu W.,Jiangsu University | Li H.,Jiangsu University
Acta Biochimica et Biophysica Sinica | Year: 2013

Recent studies have shown that microRNA-21 (miR-21) contributes to tumor resistance to chemotherapy. Interestingly, we have found that berberine could inhibit miR-21 expression in several cancer cell lines. In this study, we investigated whether berberine could modulate the sensitivity of ovarian cancer cells to cisplatin and explored the mechanism. The cisplatin-resistant SKOV3 cells that were incubated with berberine combined with cisplatin had a significantly lower survival than the cisplatin alone group and enhanced cisplatin-induced apoptosis. Berberine could inhibit miR-21 expression and function in ovarian cancer, as shown by an enhancement of its target PDCD4, an important tumor suppressor in ovarian cancer. The results suggested that berberine could modulate the sensitivity of cisplatin via regulating miR-21/PDCD4 axis in the ovarian cancer cells. © 2013 © The Author 2013. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.


Liu S.G.,Hubei Zhongshan Hospital | Ma L.,Hubei Zhongshan Hospital | Cen Q.H.,Hubei Zhongshan Hospital | Huang J.S.,Hubei Zhongshan Hospital | And 2 more authors.
Genetics and Molecular Research | Year: 2015

Genome-wide association studies in several ethnic groups have reported that polymorphisms of the telomerase reverse transcriptase (TERT) and cleft lip and palate transmembrane 1-like (CLPTM1L) genes, located on 5p15.33, are associated with susceptibility to lung cancer. However, whether genetic variants of TERT-CLPTM1L are associated with an increased risk of lung cancer in the Chinese Han population is unknown. This study examined associations between five single nucleotide polymorphisms (SNPs) of TERT-CLPTM1L (rs402710, rs401681, rs465498, rs4975616, and rs2736100) and lung cancer in a Chinese Han population in the Hubei Province. The five SNPs were detected using the Sequenom MassArray® iPLEX System in 304 lung cancer patients and 319 controls. Of the five SNPs, rs4975616 did not conform to Hardy-Weinberg equilibrium in the controls. Only rs2736100 was significantly (P = 0.034) associated with an increased risk of lung cancer. In the linkage disequilibrium analyses, a block of strong linkage disequilibrium was observed between rs401681 andrs465498 (D′ = 0.986; r2 = 0.546). No linkage disequilibrium between rs2736100 and the other three SNPs was found. In the haplotype analyses, the frequencies of the TTCT haplotype in rs402710, rs401681, rs465498, and rs2736100 differed significantly between case and control subjects (odds ratio = 0.56; 95% confidence interval, 0.36-0.88; P = 0.012). The results of this study suggested that rs2736100 on TERT-CLPTM1L indicates a poor prognosis for lung cancer in the Chinese Han population. © FUNPEC-RP.


PubMed | Hubei Zhongshan Hospital
Type: Journal Article | Journal: Acta biochimica et biophysica Sinica | Year: 2013

Recent studies have shown that microRNA-21 (miR-21) contributes to tumor resistance to chemotherapy. Interestingly, we have found that berberine could inhibit miR-21 expression in several cancer cell lines. In this study, we investigated whether berberine could modulate the sensitivity of ovarian cancer cells to cisplatin and explored the mechanism. The cisplatin-resistant SKOV3 cells that were incubated with berberine combined with cisplatin had a signicantly lower survival than the cisplatin alone group and enhanced cisplatin-induced apoptosis. Berberine could inhibit miR-21 expression and function in ovarian cancer, as shown by an enhancement of its target PDCD4, an important tumor suppressor in ovarian cancer. The results suggested that berberine could modulate the sensitivity of cisplatin via regulating miR-21/PDCD4 axis in the ovarian cancer cells.

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