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Gong W.,Hubei Zhongshan Hospital
Medical Journal of Wuhan University | Year: 2011

Objective: To investigate the relationship between homocysteine (Hcy) and carotid intimamedia thickness (CIMT). Methods: Two hundred patients were selected. High-resolution ultrasound was used to scan carotid and brachial arteries of all patients in order to check and measure CIMT, plaques of carotid arteries, and diameter of brachial arteries at rest. Patients were divided into four groups; normal carotid intima group, diffused proliferative carotid intima group, stable plaque group and unstable plaque group. Serum levels of Hcy were determined with immunological method. The relationship between the results measured by ultrasound instrument and the concentrations of Hcy was analyzed. Results; The serum levels of Hcy in proliferative carotid intima group were higher than in the normal carotid intima group (P < 0.05). The serum levels of Hcy in unstable plaque group and in stable plaque group were higher than those of diffused proliferative carotid intima group (P < 0.05). The serum levels Hcy in the unstable plaque group were higher than in stable plaque group (P < 0.05). Conclusion; The increased concentrations of hcy were closely correlated with the increment of carotid intima-media thickness and the unsteady state of plaque. Source


Wang X.-W.,Hubei Zhongshan Hospital | Zhang Y.-L.,Wuhan Institute of Tuberculosis Prevention
Genetic Testing and Molecular Biomarkers | Year: 2015

Objectives: To investigate the relationship between ABCB1 single-nucleotide polymorphisms and the efficacy of salmeterol/fluticasone combination (SFC) inhalation therapy for stable chronic obstructive pulmonary disease (COPD) in a Chinese Han population. Methods: A total of 362 patients with stable COPD were recruited between July 2012 and March 2014. Based on the therapeutic effects of lung function improvement and COPD Assessment Test (CAT) scores, all patients were either placed into the effective group (n=138) or the ineffective group (n=224). Three common polymorphisms (rs1045642C>T, rs1128503C>T, and rs1202184A>G) in the ABCB1 gene were analyzed by polymerase chain reaction-restriction fragment length polymorphism in these patients. All data were analyzed by SPSS version 18.0 software. Results: The genotype and allele frequencies of the ABCB1 rs1045642C>T polymorphic locus were significantly different between the effective group and the ineffective group under the codominant, recessive, and allele models (all p<0.05). Haplotype analysis of ABCB1 indicated that CTA (rs1045642C-rs1128503T-rs1202184A) haplotype frequencies in the effective group were significantly lower than the ineffective group (p=0.022), but TCG (rs1045642T-rs1128503C-rs1202184G) haplotype frequencies in the effective group were significantly higher than the ineffective group (p=0.048). Logistic regression analysis showed that smoking history and rs1045642 CT+CC/TT may be correlated with the efficacy of SFC inhalation therapy in stable COPD patients. Conclusion: ABCB1 rs1045642C>T polymorphism and CTA/TCG haplotypes, as well as smoking history may influence the efficacy of SFC inhalation therapy in stable COPD patients in the Chinese Han population. © Mary Ann Liebert, Inc. 2015. Source


Zhou W.,First Hospital of Wuhan City | Yang Y.,Hubei Zhongshan Hospital | Xia Z.,Huazhong University of Science and Technology | Yang X.,Huazhong University of Science and Technology | And 4 more authors.
Medical Journal of Wuhan University | Year: 2010

Objective: To explore the protective effects and mechanisms of recombinant human erythropoietin(rHuEPO) on renal tubular epithelial cell apoptosis induced by ischemia-reperfusion injury. Methods: The model of ischemia-reperfusion (I/R) injury was established through ischemia for one hour and reperfusion for two hours in cultured renal tubular epithelial (HK-2) cells. HK2 cells were cultured and then divided into five groups as following: (1) control group, (2) I/R group: receiving antimycin A 10 μmol/L and A23187 1 μmol/L, (3) pre-dosage group: I/R + rHuEPO 30 min before ischemia, (4) instant-dosage group: I/R+ rHuEPO 5 min before reperfusion, and (5) delayed-dosage group: I/R+ rHuEPO 30 min following reperfusion. The apoptotic ratio of HK-2 was monitored by FCM. The expression of Akt, GSK-3β and caspase-3 mRNA were assessed by RT-PCR and activities of Akt, GSK-3β and caspase-3 were assessed by Western blot analyses. Results: (1) Compared with control group, the apoptosis ratio of HK-2 increased, expression of phospho-Akt and phospho- GSK-3β markedly decreased and activities of Caspase-3 increased in I/R group (P<0.05). (2) Compared with I/R group, the administration of rHuEPO before ischemia or just after ischemia significantly reduced the extent of apoptotic cells, and was associated with maintenance of improved Akt (Ser473), GSK-3β (Ser9) levels and decreased Caspase-3 activity (P<0.05). Conclusion; The early administration of rHuEPO at ischemia could protect HK-2 from apoptosis induced by ischemia-reperfusion injury through Akt /GSK-3β/ caspase-3 signaling pathway. Source


Zhang J.J.,Hubei Zhongshan Hospital
Genetics and Molecular Research | Year: 2015

Genome-wide association studies in several ethnic groups have reported that polymorphisms of the telomerase reverse transcriptase (TERT) and cleft lip and palate transmembrane 1-like (CLPTM1L) genes, located on 5p15.33, are associated with susceptibility to lung cancer. However, whether genetic variants of TERT-CLPTM1L are associated with an increased risk of lung cancer in the Chinese Han population is unknown. This study examined associations between five single nucleotide polymorphisms (SNPs) of TERT-CLPTM1L (rs402710, rs401681, rs465498, rs4975616, and rs2736100) and lung cancer in a Chinese Han population in the Hubei Province. The five SNPs were detected using the Sequenom MassArray® iPLEX System in 304 lung cancer patients and 319 controls. Of the five SNPs, rs4975616 did not conform to Hardy-Weinberg equilibrium in the controls. Only rs2736100 was significantly (P = 0.034) associated with an increased risk of lung cancer. In the linkage disequilibrium analyses, a block of strong linkage disequilibrium was observed between rs401681 andrs465498 (D′ = 0.986; r2 = 0.546). No linkage disequilibrium between rs2736100 and the other three SNPs was found. In the haplotype analyses, the frequencies of the TTCT haplotype in rs402710, rs401681, rs465498, and rs2736100 differed significantly between case and control subjects (odds ratio = 0.56; 95% confidence interval, 0.36-0.88; P = 0.012). The results of this study suggested that rs2736100 on TERT-CLPTM1L indicates a poor prognosis for lung cancer in the Chinese Han population. © FUNPEC-RP. Source


Li R.,Hubei Zhongshan Hospital | Li R.,University of Chicago | Yan Z.,University of Chicago | Yan Z.,Chongqing Medical University | And 37 more authors.
International Journal of Medical Sciences | Year: 2016

Background: BMPs play important roles in regulating stem cell proliferation and differentiation. Using adenovirus-mediated expression of the 14 types of BMPs we demonstrated that BMP9 is one of the most potent BMPs in inducing osteogenic differentiation of mesenchymal stem cells (MSCs), which was undetected in the early studies using recombinant BMP9 proteins. Endogenous BMPs are expressed as a precursor protein that contains an N-terminal signal peptide, a prodomain and a C-terminal mature peptide. Most commercially available recombinant BMP9 proteins are purified from the cells expressing the mature peptide. It is unclear how effectively these recombinant BMP9 proteins functionally recapitulate endogenous BMP9. Methods: A stable cell line expressing the full coding region of mouse BMP9 was established in HEK-293 cells by using the piggyBac transposon system. The biological activities and stability of the conditioned medium generated from the stable line were analyzed. Results: The stable HEK-293 line expresses a high level of mouse BMP9. BMP9 conditioned medium (BMP9-cm) was shown to effectively induce osteogenic differentiation of MSCs, to activate BMP-R specific Smad signaling, and to up-regulate downstream target genes in MSCs. The biological activity of BMP9-cm is at least comparable with that induced by AdBMP9 in vitro. Furthermore, BMP9-cm exhibits an excellent stability profile as its biological activity is not affected by long-term storage at -80°C, repeated thawing cycles, and extended storage at 4°C. Conclusions: We have established a producer line that stably expresses a high level of active BMP9 protein. Such producer line should be a valuable resource for generating biologically active BMP9 protein for studying BMP9 signaling mechanism and functions. © 2015 Ivyspring International Publisher. Source

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