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Yang F.-M.,Hubei University of Medicine
Cancer Research and Clinic | Year: 2013

Objective: To investigate the mRNA expression of breast cancer resistance protein (BCRP) in cervical cancer (CC). Methods: The expression of BCRP mRNA was detected by real-time PCR from tissues of 12 normal and 47 cases with CC. Results: The expression of BCRP mRNA was 0.59±0.26 in CC and 0.19±0.17 in normal cervical tissues. But it was significantly higher in CC than those in normal tissues (P < 0.05). The expression of BCRP mRNA was not correlated with histological types, tumor differentiation degree and clinical stages (P > 0.05). Conclusion: BCRP mRNA over-expresses in CC, wich might play a major role in the intrinsic MDR of CC. Detection of BCRP mRNA expression may the guidance of individualized chemotherapy for patients with CC. Source

Song S.,Hubei University of Medicine
African journal of traditional, complementary, and alternative medicines : AJTCAM / African Networks on Ethnomedicines | Year: 2013

The objective of this paper was to investigate the antitumour mechanism of action of matrine by studying its inhibitory effect on gastric cancer SGC-7901 cells. SGC-7901 cells were chosen, and cell-killing capacity of matrine on gastric cancer SGC-7901 cells was determined using MTT assay and single PI staining assay. The results showed that matrine had an inhibitory effect on gastric cancer SGC-7901 cells, which was somewhat dose-dependent. The study concluded that matrine has a significant in-vitro inhibitory effect on SGC-7901 tumour cells, influences cell cycle of SGC-7901 cells, and induces their apoptosis. Source

Fu D.-G.,Hubei University of Medicine
Molecular Medicine Reports | Year: 2015

Gastric cancer is one of the most common types of cancer and the second most common cause of cancer-related mortality worldwide. An increasing number of recent studies have confirmed that gastric cancer is a multistage pathological state that arises from environmental factors; dietary factors in particulary are considered to play an important role in the etiology of gastric cancer. Improper dietary habits are one of the primary concerns as they influence key molecular events associated with the onset of gastric carcinogenesis. In the field of genetics, anticancer research has mainly focused on the various genetic markers and genetic molecular mechanisms responsible for the development of this of this disease. Some of this research has proven to be very fruitful, providing insight into the possible mechamisms repsonsible for this disease and into possible treatment modalities. However, the mortality rate associated with gastric cancer remains relatively high. Thus, epigenetics has become a hot topic for research, whereby genetic markers are bypassed and this research is directed towards reversible epigenetic events, such as methylation and histone modifications that play a crucial role in carcinogenesis. The present review focuses on the epigenetic events which play an important role in the development and progression of this deadly disease, gastric cancer. Source

Objective: To investigate the gene expression in bone mesenchymal stem cells transferred by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and its effect on C6 glioma cells in vitro. Methods: The experiment was divided into three groups, the test group transfected with TRAIL gene to BMSC, control group of BMSC transfected with empty liposomal vector, and blank control of BMSC alone. After transfering the TRAIL into GFP-BMSC with Liposomes, the expression of TRAIL was detected by RT-PCR, Western blot, and immunofluorescence. After co-culturing C6 glioma cells with GFP-BMSC-TRAIL, the bystander effect of TRAIL was detected by MTT assay, and C6 cells apoptosis was detected by immunohistochemical method. Results: GFP-BMSC-TRAIL vector was successfully constructed, with stable expression of TRAIL. The immunofluorescence showed the increased expression of TRAIL in GFP-BMSC cells at 48 h than 24 h. Western blot and PCR further revealed TRAIL expression in transfected cells, but no expression was found in control cells. In addition, compared with the blank control group and control group, GFP-BMSC-TRAIL significantly inhibited the C6 cell proliferation, with a lower survival rate (62.7 ± 0.1) % and higher apoptosis rate. Conclusion: BMSC transfected with TRAIL gene can inhibit the proliferation of C6 glioma cell, suggesting BMSC may be an effective vector for gene therapy. Source

Fu D.-G.,Hubei University of Medicine
European Review for Medical and Pharmacological Sciences | Year: 2015

Cardiovascular diseases remain one of the major health problems worldwide. The worldwide research against cardio-vascular diseases as well as genome wide association studies were successful in indentifying the loci associated with these prominent life threatening diseases but still a substantial amount of casualty remains unexplained. Over the last decade, the thorough understanding of molecular and biochemical mechanisms of cardiac disorders lead to the knowledge of various mechanisms of action of polyphenols to target inflammation during cardiac disorders. The present review article is focused on role of phytochemical resveratrol in regulation of redox signalling, autophagy and inflammation during cardiovascular pathology. Source

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