Hubei University of Medicine
Shiyan, China
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Shang L.,Hubei University of Medicine | Zhao F.,Hubei University of Medicine | Zeng B.,Hubei University of Medicine
Food Chemistry | Year: 2014

In this work, graphene oxide was reduced to graphene with an endogenous reducing agent from dimethylformamide, and then AuPd alloy nanoparticles were electrodeposited on the graphene film. The obtained AuPd-graphene hybrid film was characterized by scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction and voltammetry. The electrochemical behavior of vanillin was studied using the AuPd-graphene hybrid based electrode. It presented high electrocatalytic activity and vanillin could produce a sensitive oxidation peak at it. Under the optimal conditions, the peak current was linear to the concentration of vanillin in the ranges of 0.1-7 and 10-40 μM. The sensitivities were 1.60 and 0.170 mA mM-1 cm-2, respectively; the detection limit was 20 nM. The electrode was successfully applied to the detection of vanillin in vanilla bean, vanilla tea and biscuit samples. © 2013 Elsevier Ltd. All rights reserved.

Yuan Y.,Hubei University of Medicine | Li R.,Hubei University of Medicine | Liu Z.,Hubei University of Medicine
Analytical Chemistry | Year: 2014

Layered WS2 nanosheet is a kind of two-dimensional (2D) covalent-network solid material with remarkable structural and electronic properties that has attracted increasing interest in recent years. In this work, we propose a one-step sonication-assisted exfoliation method to prepare water-soluble WS2 nanosheet and demonstrate its application as a biosensing platform. The synthesis route is simple and straightforward. We reveal that single-strand DNA (ssDNA) chains can readily be adsorbed on the nanosheet, leading to complete and fast quenching of a fluorescent dye tagged to the DNA chain. The adsorbed ssDNA is detachable from the nanosheet upon the interaction with other biomolecules, resulting in the restoration of the fluorescence. The 2D WS2 nanosheet thus acts as an efficient platform for assembling of bioprobes. Because of the extraordinarily high quenching efficiency, which is the synergic result of both excited-state energy transfer and static quenching, the WS2 platform affords minimal background and high sensitivity. Our attempt will extend the application of this material to biosensing and probing areas. © 2014 American Chemical Society.

Yang F.-M.,Hubei University of Medicine
Cancer Research and Clinic | Year: 2013

Objective: To investigate the mRNA expression of breast cancer resistance protein (BCRP) in cervical cancer (CC). Methods: The expression of BCRP mRNA was detected by real-time PCR from tissues of 12 normal and 47 cases with CC. Results: The expression of BCRP mRNA was 0.59±0.26 in CC and 0.19±0.17 in normal cervical tissues. But it was significantly higher in CC than those in normal tissues (P < 0.05). The expression of BCRP mRNA was not correlated with histological types, tumor differentiation degree and clinical stages (P > 0.05). Conclusion: BCRP mRNA over-expresses in CC, wich might play a major role in the intrinsic MDR of CC. Detection of BCRP mRNA expression may the guidance of individualized chemotherapy for patients with CC.

Zhao L.,Hubei University of Medicine | Zhao F.,Hubei University of Medicine | Zeng B.,Hubei University of Medicine
Sensors and Actuators, B: Chemical | Year: 2013

A molecularly imprinted polymer-ionic liquid-graphene composite film coated glassy carbon electrode (MIP-IL-EGN/GCE) was presented for the first time. It was fabricated by coating a GCE with IL-graphene oxide (GO) mixture, followed by MIP suspension. The resulting electrode was then conditioned at ?1.3 V (vs SCE) in a Na2SO4 solution to make the GO turn to graphene (EGN). The MIP was prepared by free radical polymerization using methyl parathion (MP) as template, methacrylic acid as functional monomer, ethyleneglycol dimethacrylate as cross-linking reagent and 2,2μ- azobis(isobutyronitrile) as initiator. The response property of MIP-IL-EGN/GCE to MP was studied. Under the optimized conditions, the peak current of MP was linear to its concentration in the range of 0.010-7.0 μM with a sensitivity of 12.5 μA/μM, and the detection limit was 6 nM (S/N = 3). When a 1.0 μM MP solution was determined for five times using a MIP-IL-EGN/GCE, the RSD of peak current was 2.3%; the electrode-to-electrode RSD was 6.4% (n = 5). The sensor also displayed high selectivity and stability. It was applied to the determination of MP in samples and the recovery was 97-110%. © 2012 Elsevier B.V. All rights reserved.

Song S.,Hubei University of Medicine
African journal of traditional, complementary, and alternative medicines : AJTCAM / African Networks on Ethnomedicines | Year: 2013

The objective of this paper was to investigate the antitumour mechanism of action of matrine by studying its inhibitory effect on gastric cancer SGC-7901 cells. SGC-7901 cells were chosen, and cell-killing capacity of matrine on gastric cancer SGC-7901 cells was determined using MTT assay and single PI staining assay. The results showed that matrine had an inhibitory effect on gastric cancer SGC-7901 cells, which was somewhat dose-dependent. The study concluded that matrine has a significant in-vitro inhibitory effect on SGC-7901 tumour cells, influences cell cycle of SGC-7901 cells, and induces their apoptosis.

Zhou W.,Guiyang Medical University | Zhang X.-Y.,Hubei University of Medicine
American Journal of Chinese Medicine | Year: 2013

Radix isatidis (R. isatidis) (Banlangen) is a traditional Chinese medicine (TCM) famous for its broad antiviral activity. Its clinical medical history spans several thousands of years in China. Many scientists and scholars have conducted systematic research on this herb from its pharmacognosy to pharmaceuticals, especially in China. Through our research and literature reports, we inferred that the antiviral activity of R. isatidis mostly depended on the water-soluble part, including amino acids, IRPS, nucleosides, and sulfur-containing alkaloids. By playing a role in directly killing pathogenic viruses or regulating the immune system to enhance anti-virus ability, R. isatidis's biological activities mostly depend on the synergistic effect of its multiple components. This article aims to expand understanding of R. isatidis in the following aspects including medicinal resources, chemical constituents, pharmacological activities, clinical applications, and separation and analytical technologies. © 2013 World Scientific Publishing Company Institute for Advanced Research in Asian Science and Medicine.

Fu D.-G.,Hubei University of Medicine
Molecular Medicine Reports | Year: 2015

Gastric cancer is one of the most common types of cancer and the second most common cause of cancer-related mortality worldwide. An increasing number of recent studies have confirmed that gastric cancer is a multistage pathological state that arises from environmental factors; dietary factors in particulary are considered to play an important role in the etiology of gastric cancer. Improper dietary habits are one of the primary concerns as they influence key molecular events associated with the onset of gastric carcinogenesis. In the field of genetics, anticancer research has mainly focused on the various genetic markers and genetic molecular mechanisms responsible for the development of this of this disease. Some of this research has proven to be very fruitful, providing insight into the possible mechamisms repsonsible for this disease and into possible treatment modalities. However, the mortality rate associated with gastric cancer remains relatively high. Thus, epigenetics has become a hot topic for research, whereby genetic markers are bypassed and this research is directed towards reversible epigenetic events, such as methylation and histone modifications that play a crucial role in carcinogenesis. The present review focuses on the epigenetic events which play an important role in the development and progression of this deadly disease, gastric cancer.

Objective: To investigate the gene expression in bone mesenchymal stem cells transferred by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and its effect on C6 glioma cells in vitro. Methods: The experiment was divided into three groups, the test group transfected with TRAIL gene to BMSC, control group of BMSC transfected with empty liposomal vector, and blank control of BMSC alone. After transfering the TRAIL into GFP-BMSC with Liposomes, the expression of TRAIL was detected by RT-PCR, Western blot, and immunofluorescence. After co-culturing C6 glioma cells with GFP-BMSC-TRAIL, the bystander effect of TRAIL was detected by MTT assay, and C6 cells apoptosis was detected by immunohistochemical method. Results: GFP-BMSC-TRAIL vector was successfully constructed, with stable expression of TRAIL. The immunofluorescence showed the increased expression of TRAIL in GFP-BMSC cells at 48 h than 24 h. Western blot and PCR further revealed TRAIL expression in transfected cells, but no expression was found in control cells. In addition, compared with the blank control group and control group, GFP-BMSC-TRAIL significantly inhibited the C6 cell proliferation, with a lower survival rate (62.7 ± 0.1) % and higher apoptosis rate. Conclusion: BMSC transfected with TRAIL gene can inhibit the proliferation of C6 glioma cell, suggesting BMSC may be an effective vector for gene therapy.

Fu D.-G.,Hubei University of Medicine
European Review for Medical and Pharmacological Sciences | Year: 2015

Cardiovascular diseases remain one of the major health problems worldwide. The worldwide research against cardio-vascular diseases as well as genome wide association studies were successful in indentifying the loci associated with these prominent life threatening diseases but still a substantial amount of casualty remains unexplained. Over the last decade, the thorough understanding of molecular and biochemical mechanisms of cardiac disorders lead to the knowledge of various mechanisms of action of polyphenols to target inflammation during cardiac disorders. The present review article is focused on role of phytochemical resveratrol in regulation of redox signalling, autophagy and inflammation during cardiovascular pathology.

He M.,Hubei University of Medicine | Liu Z.,Hubei University of Medicine
Analytical Chemistry | Year: 2013

A paper-based microfluidic device with upconversion fluorescence assay (named as UC-μPAD) is proposed. The device is fabricated on a normal office printing sheet with a simple plotting method. Upconversion phosphors (UCPs) tagged with specific probes are spotted to the test zones on the μPAD, followed by the introduction of assay targets. Upconversion fluorescence measurements are directly conducted on the test zones after the completion of the probe-to-target reactions, without any post-treatments. The UC-μPAD features very easy fabrication and operation, simple and fast detection, low cost, and high sensitivity. UC-μPAD is a promising prospect for a clinical point-of-care test. © 2013 American Chemical Society.

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