Cai B.,Hubei University of Chinese Medicine |
Wang S.,Hubei University of Chinese Medicine |
Huang L.,Peking University |
Ning Y.,Hubei University of Chinese Medicine |
And 2 more authors.
ACS Nano | Year: 2014
A reduced graphene oxide (R-GO)-based field-effect transistor (FET) biosensor used for ultrasensitive label-free detection of DNA via peptide nucleic acid (PNA)-DNA hybridization is reported. In this work, R-GO was prepared by reduction of GO with hydrazine, and the FET biosensor was fabricated by drop-casting the R-GO suspension onto the sensor surface. PNA instead of DNA as the capture probe was employed, and DNA detection was performed through PNA-DNA hybridization by the R-GO FET biosensor. The detection limit as low as 100 fM was achieved, which is 1 order of magnitude lower than that of the previously reported graphene FET DNA biosensor based on DNA-DNA hybridization. Moreover, the R-GO FET biosensor was able to distinguish the complementary DNA from one-base mismatched DNA and noncomplementary DNA. Interestingly, the fabricated DNA biosensor was found to have a regeneration capability. The developed R-GO FET DNA biosensor shows ultrasensitivity and high specificity, indicating its potential applications in disease diagnostics as a point-of-care tool. © 2014 American Chemical Society.
Development of a novel ultrasound-assisted surfactant-enhanced emulsification microextraction method and its application to the analysis of eleven polycyclic aromatic hydrocarbons at trace levels in water
Cheng J.,Central China Normal University |
Matsadiq G.,Central China Normal University |
Liu L.,Central China Normal University |
Zhou Y.-W.,Central China Normal University |
Chen G.,Hubei University of Chinese Medicine
Journal of Chromatography A | Year: 2011
A novel ultrasound-assisted surfactant-enhanced emulsification microextraction (UASEME) technique has been proposed by using low-density extraction solvents. In the proposed technique, Tween 80 and cyclohexane were injected into 5-mL glass test tubes with conical bottoms, containing 5.00mL of a water sample that was located inside the ultrasonic bath. When the extraction process was finished, the glass test tube was sealed with a rubber plug and then placed upside down in a centrifuge. The finely dispersed droplets of cyclohexane collected at the conical bottom of test tube because the density of cyclohexane is less than of water, and the PAHs were concentrated in the cyclohexane. Next, 5μL of the cyclohexane that collected at the conical bottom was removed using a 10-μL microsyringe and injected into high performance liquid chromatography coupled with fluorescence detection (HPLC-FLD) for analysis. The proposed method avoided the use of chlorinated solvents, which have been widely used as extraction solvents in a normal UASEME assay. Parameters that affected the extraction efficiency, such as the type and volume of the extraction solvent, the type and concentration of the surfactant, and the ultrasound emulsification time and salt addition, were investigated and optimised for the method. Under the optimum conditions, the enrichment factors ranged between 90 and 247. The limits of detection of the method were 0.6-62.5ngL-1. Good recoveries and repeatability of the method for the eleven PAHs were also obtained. The proposed UASEME technique has been demonstrated to be simple, practical and environmentally friendly for the determination of PAH residues in real water samples. © 2011 Elsevier B.V.
Yang H.,Hubei University of Chinese Medicine
International Journal of Obesity | Year: 2016
Background:Increased adiposity in visceral depots is a crucial feature associated with glucocorticoid (GC) excess. The action of GCs in a target tissue is regulated by GC receptor (GR) and 11ß-hydroxysteroid dehydrogenase type 1 (11ß-HSD1) coupled with hexose-6-phosphate dehydrogenase (H6pdh). Glycogen synthase kinase-3β (GSK3β) is known to be a crucial mediator of ligand-dependent gene transcription. We hypothesized that the major effects of corticosteroids on adipose fat accumulation are in part mediated by changes in GSK3β and H6pdh.Methods:We characterized the alterations of GSK3β and GC metabolic enzymes, and determined the impact of GR antagonist mifepristone on obesity-related genes and the expression of H6pdh and 11ß-HSD1 in adipose tissue of mice exposed to excess GC as well as in in vitro studies using 3T3-L1 adipocytes treated with GCs.Results:Corticosterone (CORT) exposure increased abdominal fat mass and induced expression of lipid synthase acetyl-CoA carboxylase and ATP-citrate lyase with activation of GSK3β phosphorylation in abdominal adipose tissue of C57BL/6J mice. Increased pSer9 GSK3β was correlated with the induction of H6pdh and 11ß-HSD1. In addition, mifepristone treatment reversed the production of H6pdh and attenuated CORT-mediated production of 11ß-HSD1 and lipogenic gene expression with reduction of pSer9 GSK3β, thereby leading to improvement of phenotype of adiposity within adipose tissue in mice treated with excess GCs. Suppression of pSer9 GSK3β by mifepristone was accompanied by activation of pThr308 Akt and blockade of CORT-induced adipogenic transcriptor C/EBPα and PPARγ. In addition, mifepristone also attenuated CORT-mediated activation of IRE1α/XBP1. In addition, reduction of H6pdh by shRNA showed comparable effects to mifepristone on attenuating CORT-induced expression of GC metabolic enzymes and improved lipid accumulation in vitro in 3T3-L1 adipocytes.Conclusion:These findings suggest that elevated adipose GSK3β and H6pdh expression contribute to 11ß-HSD1 mediating hypercortisolism associated with visceral adiposity.International Journal of Obesity advance online publication, 17 May 2016; doi:10.1038/ijo.2016.57. © 2016 Macmillan Publishers Limited
Shen Y.,Hubei University of Chinese Medicine |
Jin W.,Hubei University of Chinese Medicine
Langenbeck's Archives of Surgery | Year: 2013
Purpose: The aim of our study was to evaluate the safety and effectiveness of early enteral nutrition (EN) for patients after pancreatoduodenectomy (PD). Methods: We performed a comprehensive search of abstracts in the MEDLINE database, OVID database, Springer database, the Science Citation Index, and the Cochrane Library database. Published data of randomized clinical trials (RCTs) comparing the clinically relevant outcomes of early EN and other nutritional routes for patients after PD were analyzed. The analyzed outcome variables included gastroparesis, intra-abdominal complications (gastroparesis excluded), mortality, infection, and postoperative hospital stay. The Cochrane Collaboration's RevMan 5.1 software was used for statistical analysis. Results: Four RCTs published in 2000 or later were included in this meta-analysis, in which 246 patients underwent early EN and 238 patients underwent other nutritional routes following PD. In the combined results of early EN versus other nutritional routes, no significant difference could be found in gastroparesis (odds ratio (OR), 0.89; 95 % CI, 0.36-2.18; P = 0.79), intra-abdominal complications (gastroparesis excluded) (OR, 0.82; 95 % CI, 0.53-1.26; P = 0.37), mortality (OR, 0.43; 95 % CI, 0.11-1.62; P = 0.21), infection (OR, 0.55; 95 % CI, 0.29-1.07; P = 0.08), postoperative hospital stay (mean difference, -0.93; 95 % CI, -6.51 to 4.65; P = 0.74). Conclusions: Current RCTs suggests that early EN appears safe and tolerated for patients after PD, but does not show advantages in infection and postoperative hospital stay. © 2013 Springer-Verlag Berlin Heidelberg.
Zhao M.,Hubei University of Chinese Medicine
Medicine and Law | Year: 2011
Medical disputes have been increasing in recent years in China, which cause growing tension between doctors and patients. In many locations, it has started as a practice of exploring diversified dispute settlement methods. Great importance has been attached to the non-lawsuit model through third-party mediation, which might have been led by professional organizations, insurance companies, People's Mediation Committees, or three-level governmental authorities. Those have contributed to a rapid effective resolution of medical disputes. However, there are some deficiencies that need to be addressed and fixed up, thus calling for improvement, such as the lack of a sustainable supporting mechanism, unclear legal status of the mediation institutions and mediation agreements, patching up a quarrel by only compensation. © PROBOOK 2011.
[Effects of electroacupuncture stimulation of scalp-point on cardiac sympathetic discharges, myocardial beta1-adrenoceptor protein expression and plasma norepinephrine concentration in myocardial ischemia-reperfusion injury rats].
Li M.P.,Hubei University of Chinese Medicine
Zhen ci yan jiu = Acupuncture research / [Zhongguo yi xue ke xue yuan Yi xue qing bao yan jiu suo bian ji] | Year: 2012
To explore the mechanism of electroacupuncture (EA) of scalp-point in the management of myocardial ischemia-reperfusion injury (MI/RI) by examining its effects on left cardiac sympathetic nerve activity, myocardial beta1-adrenaline receptor (AR) protein expression and plasma norepinephrine (NE) concentration in MI/RI rats. Eighteen Wistar rats were randomly divided into sham, model and EA groups (n = 6). MI/RI model was induced by ligation of the left anterior descending coronary artery for 30 min, followed by release of the ligation for 15 min. EA was applied to bilateral Epangxian I (MS 2) for 15 min. The left cardiac sympathetic nerve activity was recorded with BL-420 E+ biological signal acquisition system. Myocardial beta1-AR protein expression was examined by western blot and plasma NE level detected by enzyme-labeled immunosorbent assay. Compared with the sham group, the left cardiac sympathetic discharges and plasma NE levels and myocardial beta1-AR protein expression were markedly increased in the model group (P < 0.01), whereas in comparison with the model group, the sympathetic discharges, plasma NE level and myocardial beta1-AR protein expression in the EA group were down-regulated significantly (P < 0.05). Scalp-point EA intervention can suppress MI/RI induced increase of sympathetic nerve activity and plasma NE level, and beta1-AR protein expression, which may contribute to its effect in relieving myocardial ischemia.
Yang H.-J.,Hubei University of Chinese Medicine
Archives of Medical Research | Year: 2013
Background and Aims: A large number of studies have investigated the correlation between the interleukin (IL)-4 C-589T and C+33T polymorphisms and asthma susceptibility. However, the results are inconsistent. The objective of this study is to explore the association between the IL-4 C-589T and C+33T polymorphisms and asthma risk using meta-analysis. Methods: A total of 35 studies (31 concerning C-589T polymorphism and asthma risk with 4737 asthmatics and 6389 controls and 14 studies regarding C+33T polymorphism and asthma risk with 2544 asthmatics and 4049 controls) were included in this meta-analysis. Results: The IL-4 C-589T polymorphism was associated with increased asthma risk in a dominant genetic model (odds ratio [OR] [95% confidence interval (CI)] = 1.284 [1.131-1.459] for TT + TC vs. CC). In the subgroup analyses by ethnicity, age and atopic status of asthmatics, significantly increased risks of asthma were found both in Asians (OR [95% CI] = 1.301 [1.003-1.689]) and Caucasians (OR [95% CI] = 1.314 [1.061-1.628]) and in both adults (OR [95% CI] = 1.299 [1.098-1.537]) and children (OR [95% CI] = 1.464 [1.044-2.052]). As for the C+33T polymorphism, the results showed that it was correlated with elevated asthma risk in a recessive genetic model (OR [95% CI] = 1.744 [1.215-2.504] for TT vs. CT + CC). After stratifying analyses by ethnicity, age and atopic status of asthmatics, significantly increased asthma risks were observed in Asians (OR [95% CI] = 1.223 [1.037-1.442]), Caucasians (OR [95% CI] = 3.036 [1.224-7.529]), and children (OR [95% CI] = 1.300 [1.075-1.573]) in a recessive genetic model. Conclusions: This meta-analysis suggests that the IL-4 C-589T and C+33T polymorphisms may be risk factors for asthma. © 2013 IMSS.
Shen Y.,Hubei University of Chinese Medicine |
Jin W.,Hubei University of Chinese Medicine
Gastroenterology Research and Practice | Year: 2012
Objectives. The aim of our study was to evaluate and compare the results of pancreaticogastrostomy (PG) and pancreaticojejunostomy (PJ) after pancreaticoduodenectomy (PD). Methods. Published data of randomized clinical trials (RCTs) comparing the clinically relevant outcomes of PG versus PJ after PD were analyzed. Two reviewers assessed the quality of each trial and collected data independently. The Cochrane Collaborationâ€™s RevMan 5.0 software was used for statistical analysis. Proportions were combined, and the odds ratio (OR) with its 95% CI was used as the effect size estimate. Results. Four RCTs published in 1995 or later were included in this meta-analysis, in which 276 patients underwent PG and 277 patients underwent PJ followed PD. In the combined results of PG versus PJ, a significant difference in the morbidity of intra-abdominal complications (OR, 0.34; 95% CI, 0.23-0.49; P<0.00001) was found, but no significant difference could be found for pancreatic fistula (OR, 0.69; 95% CI, 0.42-1.12, P=0.13) mortality (OR, 1.09; 95% CI, 0.42-2.83; P=0.87), recovery with no complications (OR, 1.26; 95% CI, 0.90-1.78; P=0.18), biliary fistula (OR, 0.55; 95% CI, 0.22-1.35; P=0.19), or in delayed gastric emptying (OR, 0.55; 95% CI, 0.33-1.01; P=0.06). Conclusions. Current RCTs suggest that PG is better than PJ for pancreatic reconstruction after PD. © 2012 YinFeng Shen and WenYin Jin.
Zhang G.-J.,Hubei University of Chinese Medicine |
Ning Y.,Hubei University of Chinese Medicine
Analytica Chimica Acta | Year: 2012
Over the past decade, silicon nanowire (SiNW) biosensors have been studied for the detection of biological molecules as highly sensitive, label-free, and electrical tools. Herein we present a comprehensive review about the fabrication of SiNW biosensors and their applications in disease diagnostics. We discuss the detection of important biomarkers related to diseases including cancer, cardiovascular diseases, and infectious diseases. SiNW biosensors hold great promise to realize point-of-care (POC) devices for disease diagnostics with potential for miniaturization and integration. © 2012 Elsevier B.V.
[Effect of electroacupuncture stimulation of "Guanyuan" (CV 4), bilateral "Housanli" (ST 36), etc. on anti-fatigue ability and liver mitochondrial respiratory function in ageing rats with Yang-deficiency].
Wang H.,Hubei University of Chinese Medicine
Zhen ci yan jiu = Acupuncture research / [Zhongguo yi xue ke xue yuan Yi xue qing bao yan jiu suo bian ji] | Year: 2013
To observe the effect of electroacupuncture (EA) of "Guanyuan" (CV 4) and bilateral "Housanli" (ST 36) plus acupuncture stimulation of "Baihui" (GV 20) [an acupoint recipe for "Shuanggu Yitong" (strengthening both congenital foundation and the acquired constitution and regulating the yang-qi of the body] on the anti-fatigue ability and liver mitochondrial respiratory function in yang-deficiency ageing rats. A total of 48 male SD rats were equally and randomly divided into normal control, model, EA and EA control groups. The aged yang-deficiency model was established by subcutaneous injection of D-galactose (125 mg/kg, once daily for 40 days) and sequential muscular injection of hydrocortisone (1.5 mg/100 g, once daily for 7 days). For rats of the normal control group, subcutaneous injection of same dose of normal saline was gi-ven. EA stimulation (2 Hz, 1 mA) of "Guanyuan" (CV 4) and bilateral "Housanli"(ST 36) and manual acupuncture stimulation of "Baihui" (GV 20) were given to rats of the EA group. For rats of the EA control group, EA of "Zhongji"(CV 3) and bilateral "Yinlinquan"(SP 9), and manual acupuncture stimulation of "Yintang" (EX-HN 3) were given. The treatment was conducted once daily, 6 time a week and continuously for 4 weeks. Rats of the normal control and model groups were grabbed and fixed in the same way. The anti-fatigue ability was evaluated by using the time of exhausted swimming task and liver mitochondrial respiratory function was detected by Clark oxygen electrode. In comparison with the normal control group, the exhausted swimming time was significantly shortened in the model group (P < 0.01). Compared with the model group, the exhausted swimming time was evidently prolonged in the EA group (P < 0.01) not in the EA control group (P > 0.05). As for the liver mitochondrial respi-ratory function, the oxygen consumption rate IV was obviously higher in the model group than in the normal control group (P < 0.01), and significantly lower in the EA group than in the model group (P < 0.01). Both liver mitochondrial respiratory control rate (RCR) and ratio of phosphorus/oxgen (P/O) were significantly lower in the model group than in the normal control group (P < 0.01), and considerably higher in the EA group than in the model group (P < 0.01, P < 0.05). Comparison between the EA and EA control groups showed that the effects of the former group were evidently superior to those of the latter group in prolonging the exhausted swimming time, lowering oxygen consumption rate IV and upregulating RCR and P/O (P < 0.05). Electroacupuncture of CV 4 and ST 36 plus manual acupuncture stimulation of GV 20 can improve the anti-fatigue capability in aging rats with yang-deficiency, which may be related to its effects in reducing liver mitochondrial oxygen consumption and increasing liver mitochondrial RCR and ratio of P/O.