Hubei Research Institute of Products Quality Supervision and Inspection

Wuhan, China

Hubei Research Institute of Products Quality Supervision and Inspection

Wuhan, China
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Zhang M.,Hubei University | Liu J.,Huazhong University of Science and Technology | Kuang Y.,Philips | Li Q.,Huazhong University of Science and Technology | And 7 more authors.
International Journal of Biological Macromolecules | Year: 2017

In this work, dextran, a polysaccharide with excellent biocompatibility, is applied as the “gatekeeper” to fabricate the pH-sensitive dextran/mesoporous silica nanoparticles (MSNs) based drug delivery systems for controlled intracellular drug release. Dextran encapsulating on the surface of MSNs is oxidized by NaIO4 to obtain three kinds of dextran dialdehydes (PADs), which are then coupled with MSNs via pH-sensitive hydrazone bond to fabricate three kinds of drug carriers. At pH 7.4, PADs block the pores to prevent premature release of anti-cancer drug doxorubicin hydrochloride (DOX). However, in the weakly acidic intracellular environment (pH ∼ 5.5) the hydrazone can be ruptured; and the drug can be released from the carriers. The drug loading capacity, entrapment efficiency and release rates of the drug carriers can be adjusted by the amount of NaIO4 applied in the oxidation reaction. And from which DOX@MSN-NH-N=C-PAD10 is chosen as the most satisfactory one for the further in vitro cytotoxicity studies and cellular uptake studies. The results demonstrate that DOX@MSN-NH-N=C-PAD10 with an excellent pH-sensitivity can enter HeLa cells to release DOX intracellular due to the weakly acidic pH intracellular and kill the cells. In our opinion, the ingenious pH-sensitive drug delivery systems have application potentials for cancer therapy. © 2017 Elsevier B.V.


Luan J.,Philips | Wu K.,Philips | Li C.,Hubei University | Liu J.,Huazhong University of Science and Technology | And 5 more authors.
Carbohydrate Polymers | Year: 2017

Amphiphilic aliphatic amines grafted konjac glucomannan (KGM-g-AH8, KGM-g-AH12 and KGM-g-AH18) micelles were prepared via a simple two-step synthesis with Schiff's base as the “switch” to achieve intracellular acid-triggered curcumin release. The KGM-g-AH8 self-assembled into spherical nano-micelles (107.6 ± 11.6 nm) in an aqueous medium, and presented high curcumin loading capacity as well as good physical stability in 28 days. The in vitro curcumin release behaviors proved the controlled release property and the endosomal/lysosomal pH response of KGM-g-AH8 micelles. The cytotoxicity and cellular uptake studies were also investigated to exhibit the intracellular pH-sensitivity, safety and biocompatibility of KGM-g-AH8 micelles. This research focuses on the feasibility of KGM-based micelles to be extrapolated as promising strategies for cancer therapy and offers new potential options for intracellular drug delivery. © 2017 Elsevier Ltd


Wang S.M.,Wuhan University of Technology | Li Z.,Wuhan University of Technology | Wang X.Y.,Wuhan University of Technology | Li X.C.,Hubei Research Institute of Products Quality Supervision and Inspection
Advanced Materials Research | Year: 2014

This paper describes a new genre of Toroidal-CVT system, called the Sphere-Toroidal Continuously Variable Transmission (STCVT), which is derived from the half-toroidal traction drive (TCVT) and introduces its structure and working principle. The torque transfers from the input shaft to the cross-axle universal shaft coupling connected with the driven shaft. By discussing the difference between the torque-transfer, the paper will show the possibility of the application in the vehicle. To conclude, the system has the potential to implement infinite extension for the CVT theoretically. © (2014) Trans Tech Publications, Switzerland.


Wang X.,Huazhong University of Science and Technology | Wang X.,Huazhong Agricultural University | Dong L.-L.,Huazhong Agricultural University | Zhang C.-X.,CAS Institute of Chemistry | And 5 more authors.
Biochemical and Biophysical Research Communications | Year: 2011

In Synechocystis sp. PCC 6803, the loop domain (aa 1-70) of the phycobilisome core-membrane linker, LCM, was found to interact with the glycosyl transferase homolog, Sll1466. Growth of a Sll1466 knock-out mutant was slightly faster in low light, but strongly inhibited in high light; the phenotype is discussed in relation to the regulation of light energy transfer to photosystem II. At the molecular level, the mutant shows the following changes compared to the wild type: (1) a smaller size and higher mobility of phycobilisomes on the thylakoid membrane, and (2) a changed lipid composition of the thylakoid membrane, especially decreased amounts of digalactosyl diacylglycerol. These results indicate a profound regulatory role for Sll1466 in regulating photosynthetic energy transfer. © 2011 Elsevier Inc.


Liu J.,Wuhan University | Xu Y.,Hubei Research Institute of Products Quality Supervision and Inspection | Yang Q.,Wuhan University | Li C.,Wuhan University | And 4 more authors.
Acta Biomaterialia | Year: 2013

Novel reducible and degradable brushed poly(2-(dimethylamino) ethyl methacrylate) (PDMAEMA) derivatives were synthesized and evaluated as non-viral gene delivery vectors. First, alkyne-functionalized poly(aspartic acid) with a disulfide linker between the propargyl group and backbone poly([(propargyl carbamate)-cystamine]-a,b-aspartamide) (P(Asp-SS-AL)) was synthesized. Second, linear low molecular weight (LMW) monoazido-functionalized PDMAEMAs synthesized via atom transfer radical polymerization were conjugated to the polypeptide side-chains of P(Asp-SS-AL) via click chemistry to yield high molecular weight (HMW) polyaspartamide-based disulfide-containing brushed PDMAEMAs (PAPDEs). The PAPDEs were able to condense plasmid DNA to form 100 to 200 nm polyplexes with positive f-potentials. Moreover, in the presence of dithiothreitol the PAPDEs degraded into LMW PDAMEMA, resulting in disintegration of the PAPDE/DNA polyplexes and subsequent release of plasmid DNA. In vitro experiments revealed that the PAPDEs were less cytotoxic and more effective in gene transfection than control 25 kDa poly(ethyleneimine) and HMW linear PDMAEMA. In conclusion, reducible and degradable polycations composed of LMW PDMAEMAs coupled to a polypeptide backbone via reduction-sensitive disulfide bonds are effective gene vectors with an excellent cytocompatibility. © 2013 Acta Materialia Inc. Published by Elsevier Ltd.


Zhang M.,Hubei University | Liu J.,Huazhong University of Science and Technology | Kuang Y.,Hubei Engineering University | Li Q.,Huazhong University of Science and Technology | And 7 more authors.
Journal of Materials Chemistry B | Year: 2016

Suitable protection strategies utilized in anticancer drug delivery systems enable carriers to reach their targeted positions and release drugs intracellularly more effectively. In this study, a novel "stealthy" chitosan (CHI)/mesoporous silica nanoparticle (MSN) based complex system, named DOX@MSN-SS-CHI-PEG, was developed for tumor-triggered intracellular drug release. CHI was applied to block the pores of MSNs to prevent premature drug release, whereas mPEG was grafted on the surface of the nanoparticles via a pH-sensitive benzoic imine linker to protect the carriers. As the pH of solid tumor tissues is slightly lower than that of normal tissues, mPEG could leave the nanoparticles to expose positively charged CHI at the surface, which enabled the nanoparticles to enter cancer cells more easily. The MSNs were covered by CHI via redox-sensitive disulfide bonds. As a result, the carriers could release the drug intercellularly to kill cancer cells owing to the high concentration of glutathione (GSH) in the cytosol. In vitro drug release studies at different GSH concentrations proved the redox-sensitivity of DOX@MSN-SS-CHI-PEG. mPEG leaving studies demonstrated that mPEG could leave the nanoparticles effectively at pH 6.0. The cytotoxicity and cell internalization behavior were also investigated in detail. In conclusion, the novel DOX@MSN-SS-CHI-PEG drug delivery system, which was "stealthy" in the physiological environment at pH 7.4 because of the protection of mPEG, was "activated" in weakly acidic tumor tissues to achieve tumor-triggered intracellular drug release; this system has great potential for cancer therapy. © 2016 The Royal Society of Chemistry.


Zhou L.,Wuhan University of Technology | Zhao M.Y.,Wuhan University of Technology | Wang X.Y.,Wuhan University of Technology | Li X.C.,Hubei Research Institute of Products Quality Supervision and Inspection
Advanced Materials Research | Year: 2014

The battery ability of recovering electricity plays a significant role in improving the regenerative braking performance. In this paper, a control for recovery of braking energy in Electric Vehicles (EVs) with electro hydraulic brake (EHB) is proposed, which makes the recovery transfer to the electric hydraulic pump of EHB directly, rather than being stored statically in the battery. An energy flow strategy was designed for the maximum braking energy recovery based on this control. The simulation results show higher energy recovery rate in comparison to the general recycling control. © (2014) Trans Tech Publications, Switzerland.


Wang X.Y.,Wuhan University of Technology | Wang S.M.,Wuhan University of Technology | Zhou L.,Wuhan University of Technology | Li X.C.,Hubei Research Institute of Products Quality Supervision and Inspection
Advanced Materials Research | Year: 2014

The HE (heat exchanger) of automotive exhaust TEG (thermoelectric generator) plays a vital role in converting thermal energy into electrical energy, therefore achieves the goal of energy conservation and emission reduction. A new regular-octagon HE is modeled and simulated in FLUENT to obtain the velocity path-lines of fluid. Through simulation results, the velocity path-line is tracked and analyzed, in order to evaluate the fluid resistance and fluid turbulence intensity. By using control variable method, a variety of HE models with different structural parameters are modeled and simulated, to optimize the structure of HE. Eventually, it is intended to design a HE with proper fluid resistance and remarkable turbulence intensity. © (2014) Trans Tech Publications, Switzerland.


Chen H.,Hubei University | Zheng D.,Hubei University | Liu J.,Huazhong University of Science and Technology | Kuang Y.,Hubei Engineering University | And 7 more authors.
International Journal of Biological Macromolecules | Year: 2016

In this work, a novel pH-sensitive drug delivery system based on modified dextrin coated mesoporous silica nanoparticles (MSNs), DOX@MSN-DDA-CL, are prepared. The dextrin grafting on the surface of MSNs is oxidized by KIO4 to obtain dextrin dialdehyde, which is then cross-linked by tetraethylenepentamine through a pH-sensitive Schiff's base. Under physiological conditions, the cross-linked dextrin dialdehyde blocks the pores to prevent premature release of model drug doxorubicin hydrochloride (DOX). In the weak acidic environment, pH 6.0 in this work, the Schiff's base can be hydrolyzed and released the drug. The in vitro drug release studies at different pHs prove the pH-sensitivity of DOX@MSN-DDA-CL. The cytotoxicity and cell internalization behavior are also investigated in detail. In vivo tissue distribution and pharmacokinetics with a H22-bearing mouse animal mode are also studied, prove that DOX@MSN-DDA-CL has a longer retention time than that of pure DOX and can accumulate in tumor region via enhanced permeation and retention and nanomaterials-induced endothelial cell leakiness effects. In conclusion, the pH-sensitive modified dextrin/MSNs complex drug delivery system has a great potential for cancer therapy. © 2016 Elsevier B.V..


PubMed | Hubei University, Hubei Engineering University, Hubei Research Institute of Products Quality Supervision and Inspection and Huazhong University of Science and Technology
Type: | Journal: International journal of biological macromolecules | Year: 2016

In this work, a novel pH-sensitive drug delivery system based on modified dextrin coated mesoporous silica nanoparticles (MSNs), DOX@MSN-DDA-CL, are prepared. The dextrin grafting on the surface of MSNs is oxidized by KIO4 to obtain dextrin dialdehyde, which is then cross-linked by tetraethylenepentamine through a pH-sensitive Schiffs base. Under physiological conditions, the cross-linked dextrin dialdehyde blocks the pores to prevent premature release of model drug doxorubicin hydrochloride (DOX). In the weak acidic environment, pH 6.0 in this work, the Schiffs base can be hydrolyzed and released the drug. The in vitro drug release studies at different pHs prove the pH-sensitivity of DOX@MSN-DDA-CL. The cytotoxicity and cell internalization behavior are also investigated in detail. In vivo tissue distribution and pharmacokinetics with a H22-bearing mouse animal mode are also studied, prove that DOX@MSN-DDA-CL has a longer retention time than that of pure DOX and can accumulate in tumor region via enhanced permeation and retention and nanomaterials-induced endothelial cell leakiness effects. In conclusion, the pH-sensitive modified dextrin/MSNs complex drug delivery system has a great potential for cancer therapy.

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