Entity

Time filter

Source Type


Yang X.,Huazhong Agricultural University | Wang L.,Hubei Provincial Center for Diseases Control and Prevention | Feng H.,Hubei Entry Exit Inspection and Quarantine Bureau | Qi M.,Huazhong Agricultural University | And 6 more authors.
Parasitology Research | Year: 2016

Gastrodiscidae species are neglected but significant paramphistomes in small ruminants, which can lead to considerable economic losses to the breeding industry of livestock. However, knowledge about molecular ecology, population genetics, and phylogenetic analysis is still limited. In the present study, we firstly sequenced and analyzed the full mitochondrial (mt) genome of Homalogaster paloniae (14,490 bp). The gene contents and organization of the H. paloniae mt genome is the same as that of other digeneans, such as Fasciola hepatica and Paramphistomum cervi. It is interesting that unlike other paramphistomes, H. paloniae is flat in shape which is similar with Fasciola, such as F. hepatica. Phylogenetic analysis of H. paloniae and other 17 selected digeneans using concatenated amino acid sequences of the 12 protein-coding genes showed that Gastrodiscidae is closely related to Paramphistomidae and Gastrothylacidae. The availability of the mt genome sequence of H. paloniae should provide an important foundation for further molecular study of Gastrodiscidae and other digeneans. © 2016 Springer-Verlag Berlin Heidelberg Source


Yang X.,Huazhong Agricultural University | Zhao Y.,Huazhong Agricultural University | Wang L.,Hubei Provincial Center for Diseases Control and Prevention | Feng H.,Hubei Entry Exit Inspection and Quarantine Bureau | And 5 more authors.
Parasites and Vectors | Year: 2015

Background: Fischoederius elongates is an important trematode of Paramphistomes in ruminants. Animals infected with F. elongates often don't show obvious symptoms, so it is easy to be ignored. However it can cause severe economic losses to the breeding industry. Knowledge of the mitochondrial genome of F. elongates can be used for phylogenetic and epidemiological studies. Findings: The complete mt genome sequence of F. elongates is 14,120 bp in length and contains 12 protein-coding genes, 22 tRNA genes, two rRNA genes and two non-coding regions (LNR and SNR). The gene arrangement of F. elongates is the same as other trematodes, such as Fasciola hepatica and Paramphistomum cervi. Phylogenetic analyses using concatenated amino acid sequences of the 12 protein-coding genes by Maximum-likelihood and Neighbor-joining analysis method showed that F. elongates was closely related to P. cervi. Conclusion: The complete mt genome sequence of F. elongates should provide information for phylogenetic and epidemiological studies for F. elongates and the family Paramphistomidae. © 2015 Yang et al.; licensee BioMed Central. Source


Yang X.,Huazhong Agricultural University | Gasser R.B.,University of Melbourne | Koehler A.V.,University of Melbourne | Wang L.,Hubei Provincial Center for Diseases Control and Prevention | And 5 more authors.
Parasites and Vectors | Year: 2015

Background: Hypoderaeum conoideum is a neglected but important trematode. The life cycle of this parasite is complex: snails serve as the first intermediate hosts: bivalves, fishes or tadpoles serve as the second intermediate hosts, and poultry (such as chickens and ducks) act as definitive hosts. In recent years, H. conoideum has caused significant economic losses to the poultry industry in some Asian countries. Despite its importance, little is known about the molecular ecology and population genetics of this parasite. Knowledge of mitochondrial (mt) genome of H. conoideum can provide a foundation for phylogenetic studies as well as epidemiological investigations. Methods: The entire mt genome of H. conoideum was amplified in five overlapping fragments by PCR and sequenced, annotated and compared with mt genomes of selected trematodes. A phylogenetic analysis of concatenated mt amino acid sequence data for H. conoideum, eight other digeneans (Clonorchis sinensis, Fasciola gigantica, F. hepatica, Opisthorchis felineus, Schistosoma haematobium, S. japonicum, S. mekongi and S. spindale) and one tapeworm (Taenia solium; outgroup) was conducted to assess their relationships. Results: The complete mt genome of H. conoideum is 14,180 bp in length, and contains 12 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and one non-coding region (NCR). The gene arrangement is the same as in Fasciola spp, with all genes being transcribed in the same direction. The phylogenetic analysis showed that H. conoideum had a relatively close relationship with F. hepatica and other members of the Fasciolidae, followed by the Opisthorchiidae, and then the Schistosomatidae. Conclusions: The mt genome of H. conoideum should be useful as a resource for comparative mt genomic studies of trematodes and for DNA markers for systematic, population genetic and epidemiological studies of H. conoideum and congeners. © 2015 Yang et al.; licensee BioMed Central. Source


Song Q.,Huazhong Agricultural University | Wang L.,Huazhong Agricultural University | Wang L.,Hubei Provincial Center for Diseases Control and Prevention | Fang R.,Huazhong Agricultural University | And 3 more authors.
Tropical Animal Health and Production | Year: 2012

Mycoplasma wenyonii is a wall-less hemotrophic prokaryote with worldwide distribution. This paper describes the development of a LAMP method targeting 16S rRNA for specific detection of M. wenyonii in its vectors and cattle. The LAMP method is specific for M. wenyonii detection and more sensitive than PCR. A total of 330 blood samples from cattle were tested by LAMP and PCR detection, 71 (21.5 %) samples were positive by the LAMP, while only 62 (18.8 %) were positive by PCR. For detecting transmission vectors, 26 lice, 30 flies, and 26 mosquitoes were collected and 18 lice, 20 flies, and 21 mosquitoes were tested positive by LAMP and PCR. These results indicate that the LAMP assay is a simple and convenient diagnostic tool for M. wenyonii detection and can be used in epidemiological surveys. © 2012 Springer Science+Business Media B.V. Source


Chen L.,Huazhong Agricultural University | Feng Y.,Yangtze University | Chen H.-M.,Wuhan University of Technology | Wang L.-X.,Hubei Provincial Center for Diseases Control and Prevention | And 4 more authors.
Parasitology Research | Year: 2016

Clinostomum complanatum is an important trematode in fishes, birds of the family Ardeidae, and humans. Until now, limited knowledge is available regarding its molecular epidemiology, ecology, and phylogenetic study. Knowledge of the full mitochondrial genome of C. complanatum will provide important information for the study of epidemiology, biology, and genetic diversity of this fluke. In the present study, the complete mitochondrial genome of C. complanatum was sequenced and analyzed. The complete C. complanatum mitochondrial genome is 13,796 bp in length and contains 12 protein-coding genes, 22 tRNA genes, two rRNA genes, and one non-coding control region. All the 12 protein-coding genes are transcribed in the same direction and are AT-rich. Phylogenetic analysis based on concatenated amino acid sequences of the 12 protein-coding genes from C. complanatum and other selective digeneans showed that C. complanatum is in a separate branch, indicating C. complanatum has no closer relationship with any of the selected families. The complete mtDNA sequence of C. complanatum will increase our knowledge of mitochondrial genomics data and will also provide an important resource for studies of inter- and intra-species variation of flukes belonging to Clinostomidae. © 2016 Springer-Verlag Berlin Heidelberg Source

Discover hidden collaborations