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PubMed | Hubei Provincial Academy of Preventive Medicine, U.S. Center for Disease Control and Prevention, Yangxing Municipal Station of Schistosomiasis Control and Zhongxiang Municipal Station of Schistosomiasis Control
Type: Evaluation Studies | Journal: Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control | Year: 2013

To evaluate the molluscicidal effect of the plastic film mulch covering method.In the barren land with a lot of Oncomelania hupensis snails, 3 experimental groups were set up, in which respectively using 3 different methods: a simple plastic film mulch covering method, film assisted with ammonium bicarbonate, and film assisted with amine molluscicide. In the canals, two experimental groups were set up, in which respectively using 2 different methods: simple plastic film mulch covering method and film assisted with ammonium bicarbonate. In above-mentioned areas, obstacles were cleared artificially. In each experiment group, 4 observation spots were set up, and 100 living snails were pot in each spot, and then, the plastic film mulch was evenly covered. The status of the snails was observed 3, 7, 15 and 30 days after the intervention.In the barren land, the snail death rates of the simple plastic film group were 20.77% and 96.92% 15 days and 30 days after the intervention, respectively. The snail death rate of the film assisted with amine molluscicide group was 80.46% 3 days after the intervention. In all the experiment groups, the snail death rates were from 96.92 to 100% 30 days after the intervention.The plastic film mulch covering method has a great molluscicide effect in the hill subtype and water network type of schistosomiasis epidemic areas.


Ai H.,Central China Normal University | Wang F.,Huazhong Agricultural University | Zhang N.,Central China Normal University | Zhang L.,Hubei Provincial Academy of Preventive Medicine | Lei C.,Huazhong Agricultural University
Journal of Insect Science | Year: 2013

In our previous study, protein-enriched fraction (PEF) that was isolated from the larvae of the housefly, Musca domestica L. (Diptera: Muscidae), showed excellent hepatoprotective activity as well as the potential for clinical application in therapy for liver diseases. In this study, antiviral, immunomodulatory, and free radical scavenging activities of PEF were evaluated. The antiviral results demonstrated that PEF inhibited the infection of avian influenza virus H9N2 and had a virucidal effect against the multicapsid nucleopolyhedrovirus of the alfalfa looper, Autographa californica Speyer (Lepidoptera: Noctuidae) in vitro. The mortality of silkworm larve in a PEF treatment group decreased significantly compared with a negative control. PEF showed excellent scavenging activity for 1,1-diphenyl-2-picrylhydrazyl and superoxide anion radicals, which were similar to those of ascorbic acid. The imunomodulatory results suggested that PEF could effectively improve immune function in experimental mice. Our results indicated that PEF could possibly be used for the prophylaxis and treatment of diseases caused by avian influenza virus infection. In addition, PEF with virucidal activity against insect viruses might provide useful for the development of antimicrobial breeding technology for economically important insects. As a natural product from insects, PEF could be a potential source for the discovery of potent antioxidant and immunomodulatory agents. © This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.


Zhou M.,Huazhong Agricultural University | Wang L.,Huazhong Agricultural University | Zhou S.,Huazhong Agricultural University | Wang Z.,Huazhong Agricultural University | And 7 more authors.
Oncotarget | Year: 2015

Developing efficacious oral rabies vaccines is an important step to increase immunization coverage for stray dogs, which are not accessible for parenteral vaccination. Our previous studies have demonstrated that recombinant rabies virus (RABV) expressing cytokines/chemokines induces robust protective immune responses after oral immunization in mice by recruiting and activating dendritic cells (DCs) and B cells. To develop an effective oral rabies vaccine for dogs, a recombinant attenuated RABV expressing dog GM-CSF, designated as LBNSE-dGM-CSF was constructed and used for oral vaccination in a dog model. Significantly more DCs or B cells were activated in the peripheral blood of dogs vaccinated orally with LBNSE-dGM-CSF than those vaccinated with the parent virus LBNSE, particularly at 3 days post immunization (dpi). As a result, significantly higher levels of virus neutralizing antibodies (VNAs) were detected in dogs immunized with LBNSE-dGM-CSF than with the parent virus. All the immunized dogs were protected against a lethal challenge with 4500 MICLD50 of wild-type RABV SXTYD01. LBNSE-dGM-CSF was found to replicate mainly in the tonsils after oral vaccination as detected by nested RT-PCR and immunohistochemistry. Taken together, our results indicate that LBNSE-dGM-CSF could be a promising oral rabies vaccine candidate for dogs.


Yang P.,Huazhong University of Science and Technology | Zeng Q.,Huazhong University of Science and Technology | Cao W.-C.,Hubei Provincial Academy of Preventive Medicine | Wang Y.-X.,Huazhong University of Science and Technology | And 4 more authors.
Environmental Research | Year: 2016

Trihalomethanes (THMs) have been reported to be associated with altered semen quality, and this association may be modified by inherited differences in cytochrome P450 (CYP2E1) and glutathione S-transferase (GSTZ1 and GSTT1), which metabolize THMs. We conducted a cross-sectional study to examine the interactions between CYP2E1, GSTZ1 and GSTT1 polymorphisms and exposure to THMs on semen quality among 401 men from the Reproductive Center of Tongji Hospital in Wuhan China. The baseline blood concentrations of four individual THMs, chloroform (TCM), bromodichloromethane (BDCM), dibromochloromethane (DBCM) and bromoform (TBM), were measured as biomarkers of exposure to drinking water THMs. Genotypes were determined by real-time PCR, and semen-quality parameters were evaluated according to the World Health Organization guidelines. GSTT1 genotype significantly modified the association between exposure to Br-THMs (sum of BDCM, DBCM and TBM) and below-reference sperm motility (Pint=0.02). Men with above-median blood Br-THM levels had an increased odds ratio (OR) of below-reference sperm compared to men with below-median blood Br-THM levels (OR=2.15, 95% CI: 1.11, 4.19) in the GSTT1 null genotype only. In addition, we found that men with a TT of CYP2E1 rs 915,906 had higher blood TCM and TTHM (sum of TCM, BDCM, DBCM and TBM) concentrations than men with a CT/CC of CYP2E1 rs 915,906. Our results suggest that GSTT1 polymorphisms modify Br-THM exposure relation with semen quality, and CYP2E1 polymorphisms are associated with internal levels of exposure to THMs. © 2016.


Liu H.-C.,Hubei Provincial Academy of Preventive Medicine | Zhong C.-H.,Hubei Provincial Academy of Preventive Medicine | Liao S.-Q.,Hubei Provincial Academy of Preventive Medicine | He H.,Hubei Provincial Academy of Preventive Medicine
Chinese Journal of Schistosomiasis Control | Year: 2014

Methods: By using a combination of quantitative and qualitative methods, the institutions of schistosomiasis prevention and control at county and township levels were investigated for the institutional setup, staffing and fulfillment functions since the reform of 2004.Objective: To investigate the current situation of management of institutions of schistosomiasis prevention and control in Hubei Province, so as to explore the probable competency building standards for these institutions at the county and township levels.Results: Among 63 schistosomiasis endemic counties (cities, districts) of Hubei Province, there were 26 independent schistosomiasis control institutions (41.27%), there were 24 institutions which were incorporated into CDC (38.10%), and there were no institutions in 13 counties (20.63%). Among 518 endemic towns, there were 299 institutions (57.72%). The total staffing size were 1 932, but there were 1 586 (82.09%) people actually working in the post, and therefore there were 346 (17.91%) empty positions. The average rates of carrying out the six functions were 91.48%-71.19%, but only 19.23% of the institutions participated in the comprehensive schistosomiasis control management project and its effect assessment.Conclusion: According to the management model for schistosomiasis control institutions under the current institutional mechanisms, we need a rigorous industry standard to constrain, guide and standardize the management and capacity-building of the institutions in different historical periods.


Sun L.,Huazhong University of Science and Technology | Huang X.-B.,Huazhong University of Science and Technology | Suo J.-P.,Huazhong University of Science and Technology | Fan B.-L.,Hubei Provincial Academy of Preventive Medicine | And 3 more authors.
Fertility and Sterility | Year: 2011

Objective: To investigate the biocompatibility of a novel copper-containing composite to provide preclinical data for clinical application of intrauterine device (IUD) or intra-vas device (IVD). Design: Prospective experimental study. Setting: Good laboratory practices laboratories. Animals: Twenty healthy adult mice (SPF grade Kunming white mice, animal code SCXK 2003-0005). Intervention(s): Cytotoxicity tests in vitro were conducted to evaluate the influence of the materials on the morphology, growth, and proliferation of cultured L929 mouse fibroblasts. Acute systemic toxicity tests were conducted to investigate the acute systemic toxic reaction with mice, and then the materials were implanted into the spinal muscle of rabbits (n = 15). The rabbits were sacrificed for pathologic examination at 1, 4, and 12 weeks after surgery. Main Outcome Measure(s): Evaluation of cytotoxicity by MTT assay, cytotoxicity test by direct contact assay, acute systemic toxicity test, and material implantation test. Result(s): The cytotoxicity grade of the copper-containing composite was 0-1, suggesting that the material was free of cytotoxicity; no acute systemic toxicity was found in any mice; mild inflammatory reaction was observed in the surrounding tissues of the implanted material in the early implantation stage, which was similar to that of the sham-operated sides. Twelve weeks after implantation, the inflammatory reaction was completely disappeared in the implanted tissue, similarly to the sham-operated sides. The fibrosis membrane surrounding the material became stable gradually over time. Conclusion: The copper-containing composite has excellent biocompatibility, which is feasible and safe for the clinical application as a novel contraceptive material. © 2011 American Society for Reproductive Medicine, Published by Elsevier Inc.


Liu H.-C.,Hubei Provincial Academy of Preventive Medicine | Liao S.-Q.,Hubei Provincial Academy of Preventive Medicine | Zhong C.-H.,Hubei Provincial Academy of Preventive Medicine | He H.,Hubei Provincial Academy of Preventive Medicine
Chinese Journal of Schistosomiasis Control | Year: 2014

Objective: To understand the human resources of the grassroots institutions of schistosomiasis control and prevention, so as to provide the evidence for formulating the standards of institutional capacity-building. Methods: By using the combination of quantitative and qualitative methods, the hierarchy of schistosomiasis control institution workers, structural features of workers, and benefits of workers were investigated and the results were analyzed statistically after the 2004 reform. Results: The constituent ratios of personnel ≤S30 years old, 30 to 45 years old, and 545 years old were 6.8%, 64.0% and 29.2% respectively, with an average age of 43.1 years. For education levels, 61.35% of the personnel had secondary or high school levels. At the city level, the structural proportion of the senior professional; medium professional and primary professional titles was 1.4:5.6:3.0, and at the county level, the proportion was 0.5:6.1:3.4. There was 14 200 yuan per capita at the township schistosomiasis control institutions. Conclusion: The technology of the personnel in schistosomiasis institutions of Hubei Province is weak, the average age of personnel is old, and the salary is low. © 2014, Editorial Office of Chinese Journal of Schistosomiasis Control. All rights reserved.


PubMed | Hubei Provincial Academy of Preventive Medicine and Huazhong Agricultural University
Type: Journal Article | Journal: Oncotarget | Year: 2015

Developing efficacious oral rabies vaccines is an important step to increase immunization coverage for stray dogs, which are not accessible for parenteral vaccination. Our previous studies have demonstrated that recombinant rabies virus (RABV) expressing cytokines/chemokines induces robust protective immune responses after oral immunization in mice by recruiting and activating dendritic cells (DCs) and B cells. To develop an effective oral rabies vaccine for dogs, a recombinant attenuated RABV expressing dog GM-CSF, designated as LBNSE-dGM-CSF was constructed and used for oral vaccination in a dog model. Significantly more DCs or B cells were activated in the peripheral blood of dogs vaccinated orally with LBNSE-dGM-CSF than those vaccinated with the parent virus LBNSE, particularly at 3 days post immunization (dpi). As a result, significantly higher levels of virus neutralizing antibodies (VNAs) were detected in dogs immunized with LBNSE-dGM-CSF than with the parent virus. All the immunized dogs were protected against a lethal challenge with 4500 MICLD50 of wild-type RABV SXTYD01. LBNSE-dGM-CSF was found to replicate mainly in the tonsils after oral vaccination as detected by nested RT-PCR and immunohistochemistry. Taken together, our results indicate that LBNSE-dGM-CSF could be a promising oral rabies vaccine candidate for dogs.


Yuan L.,Huazhong University of Science and Technology | Li Y.,Huazhong University of Science and Technology | Li G.,Huazhong University of Science and Technology | Song Y.,Huazhong University of Science and Technology | Gong X.,Hubei Provincial Academy of Preventive Medicine
Journal of Endocrinological Investigation | Year: 2013

Aims: Pancreatic microcirculation plays a pivotal role in the physiological function and survival of β-cells. Ang(1-7) is a novel component of the renin angiotensin system (RAS) that has beneficial effects on microcirculation. In the present study, we investigated the effects of systemic Ang(1-7) administration (with or without its receptor Mas antagonist A-779) on pancreatic microcirculation and β-cell function. Methods: These effects were studied in vivo using a rat model of Type 2 diabetes (T2DM). Pancreatic microcirculation and islet microvessel density were measured; and β-cell function, insulin content, and the apoptosis of islet cells were assessed, respectively. Additionally, we evaluated endothelial nitric oxide synthase (eNOS) expression and nitric oxide (NO) concentration in islets. Results: After Ang(1-7) intervention, pancreatic microcirculation and intra-islet microvessel density were significantly improved (p<0.05), and more importantly, first-phase insulin secretion of β-cells as well as relative insulin content in islets were increased, and the amount of apoptotic islet cells was decreased (p<0.05). And eNOS expression and NO release were up-regulated in pancreatic islets by Ang(1-7) administration (p<0.05). These positive effects of Ang(1-7) were prevented by the addition of A-779 (p<0.05). Conclusions: Our findings suggest that systemic Ang(1-7) treatment could attenuate β-cell dysfunction and ameliorate islet cell apoptosis in T2DM rats by improving pancreatic microcirculation, perhaps through the mechanism of endothelial vasodilation. ©2013, Editrice Kurtis.


PubMed | Hubei Provincial Academy of Preventive Medicine and Huazhong Agricultural University
Type: Journal Article | Journal: Archives of virology | Year: 2016

Two recombinant rabies viruses overexpressing their glycoprotein (G) were compared in this study, with the overexpressed G inserted between P and M genes (named LBNSE-PM-G), and between the G and L genes (named LBNSE-GL-G), respectively. LBNSE-PM-G produced more G protein and induced stronger apoptosis than LBNSE-GL-G in infected cells, while the amount of virion-incorporated G in LBNSE-PM-G was less than in LBNSE-GL-G. Mice immunized with inactivated LBNSE-PM-G produced lower titers of virus-neutralizing antibody, and this recombinant conferred worse protection than LBNSE-GL-G. Our results suggest that over expressed G gene inserted between G and L, but not between P and M, enhanced the immunogenicity when used as an inactivated rabies vaccine.

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