Hubei Provincial Academy of Agricultural science

Wuhan, China

Hubei Provincial Academy of Agricultural science

Wuhan, China

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Wu J.J.,Huazhong Agricultural University | Wu J.J.,Hubei Provincial Academy of Agricultural science | Song L.J.,Huazhong Agricultural University | Wu F.J.,Huazhong Agricultural University | And 9 more authors.
Molecular Biology Reports | Year: 2013

Cattle and water buffalo belong to the same subfamily Bovinae and share chromosome banding and gene order homology. In this study, we used genome-wide Illumina BovineSNP50 BeadChip to analyze 91 DNA samples from three breeds of water buffalo (Nili-Ravi, Murrah and their crossbred with local GuangXi buffalos in China), to demonstrate the genetic divergence between cattle and water buffalo through a large single nucleotide polymorphism (SNP) transferability study at the whole genome level, and performed association analysis of functional traits in water buffalo as well. A total of 40,766 (75.5 %) bovine SNPs were found in the water buffalo genome, but 49,936 (92.5 %) were with only one allele, and finally 935 were identified to be polymorphic and useful for association analysis in water buffalo. Therefore, the genome sequences of water buffalo and cattle shared a high level of homology but the polymorphic status of the bovine SNPs varied between these two species. The different patterns of mutations between species may associate with their phenotypic divergence due to genome evolution. Among 935 bovine SNPs, we identified a total of 9 and 7 SNPs significantly associated to fertility and milk production traits in water buffalo, respectively. However, more works in larger sample size are needed in future to verify these candidate SNPs for water buffalo. © 2012 Springer Science+Business Media Dordrecht.


Wu J.,Hubei Provincial Academy of Agricultural science | Peng X.,Hubei Provincial Academy of Agricultural science | Zhou A.,Huazhong Agricultural University | Qiao M.,Hubei Provincial Academy of Agricultural science | And 6 more authors.
Molecular and Cellular Biochemistry | Year: 2014

Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically important diseases of swine, which is caused by PRRS virus (PRRSV). CD151, one of PRRSV entry mediators, determines the cell susceptibility for PRRSV. Emerging evidence indicates that the host microRNAs (miRNAs) play key roles in modulating virus infection and viral pathogenesis. In the present study, targeting porcine CD151 miRNAs were identified, and their function during PRRSV infection in MARC-145 cells was further verified. We found that miR-506 could directly target porcine CD151 3′-UTR mRNA by luciferase reporter assay. Overexpression of miR-506 significantly decreased CD151 expression at both mRNA and protein levels. Furthermore, overexpression of miR-506 reduced cellular PRRSV replication and virus release in MARC-145 cells. Our results suggested that miR-506 could inhibit PRRSV replication by directly targeting PRRSV receptor of CD151 in MARC-145 cells. However, the molecular mechanisms of miR-506 and its function in vivo need further investigation. © 2014, Springer Science+Business Media New York.


Mokhele B.,Huazhong Agricultural University | Zhan X.,Hubei Provincial Academy of Agricultural Science | Yang G.,Huazhong Agricultural University | Zhang X.,Huazhong Agricultural University
Canadian Journal of Plant Science | Year: 2012

In this review we discuss mainly nitrogen assimilation in crop plants and factors affecting the related process. Nitrogen is a major macro-element limiting the growth and development of plants in agriculture. Both organic and inorganic forms of nitrogen are metabolized in plants; nitrate and ammonia in soil are common forms of inorganic nitrogen that can be metabolized in all plants. There are other nitrogen forms, which include amino acids, nitrite and urea, that are metabolized in plants. Metabolism normally starts with reduction of nitrate to nitrite, and the latter further reduces to form ammonium with the presence of relevant enzymes. This reaction occurs more rapidly in leaves in the presence of light. After ammonia is formed, it enters into the biosynthetic pathways of plant cells, such as reductive amination and transpiration, to produce different amino acids. Amino acids in cells take part in the synthesis of protein and other nitrogenous compounds that help in body building. Radiation, gaseous factors, the presence of metals, soil pH and amount of nitrate are some of the environmental factors affecting absorption and reduction of nitrogen in plants. This review presents a comprehensive understanding of the assimilation process by crop plants of nitrogen and recommends that favorable surrounding conditions are the prerequisites for plants to absorb and utilize nitrogen efficiently.


Wu J.J.,Hubei Provincial Academy of Agricultural science | Gao J.,Huazhong Agricultural University | Tong Q.,University of Cambridge | Affara N.A.,University of Cambridge | And 7 more authors.
Animal Science Papers and Reports | Year: 2013

Zygote arrest 1 (ZAR1) is oocyte-specific protein involved in the initiation of embryo development. The objectives of this study were to identify novel mutations in ZAR1 gene and investigate the association between its genetic variants and litter size in Chinese local and European pigs. A novel single nucleotide polymorphism (SNP) in exon 3 (g.2612T>C) and a 5 bp deletion/insertion in intron 3 (g.3838-3839insTGCAG) were found by sequencing and then their genotypes were identified in 218 sows of five breeds. The results of association analysis showed that the SNP g.2612T>C was significantly related to litter size in Durocs (P<0.05), the sows with genotype CC genotype gave more piglets than those of TT or/and TC genotypes. What's more, the litter size in the Duroc breed was also significantly dependent (P<0.05) on polymorphism in intron3 (g.3838-3839 sTGCAG). Sows with NN genotype had more piglets per litter than those of MM or/and MN genotypes. A similar relation (though not significant) was observed in remaining breeds tested. It is concluded that ZAR1 gene might be a potential important candidate gene related to litter size in pigs.


Zhou A.,Huazhong Agricultural University | Li S.,Huazhong Agricultural University | Wu J.,Hubei Provincial Academy of Agricultural science | Khan F.A.,Huazhong Agricultural University | Zhang S.,Huazhong Agricultural University
Virus Research | Year: 2014

Antimicrobial response is greatly influenced by microRNAs (miRNAs) which are the important post-transcriptional regulators of gene expression. Simultaneously, host pathogen recognition receptors (PRRs) engaged by pathogen-associated molecular patterns (PAMPs) also play critical roles in activating innate immunity against microbial infection. Emerging evidences suggest that the interaction between microbial-regulated miRNAs and important PRRs signaling pathways influence host immune response to microbial pathogens. In this manuscript, we describe the roles of miRNAs in virus-regulated innate immune pathways and the crosstalk between miRNAs and PRRs, further breaking out the mechanistic dissection of miRNAs-PRRs in viral infection and the development of the prognosis of disease and novel miRNA-therapeutic strategies targeting immunity. © 2014 Elsevier B.V.


Wu J.J.,Hubei Provincial Academy of Agricultural science | Peng X.W.,Hubei Provincial Academy of Agricultural science | Li F.E.,Huazhong Agricultural University | Qiao M.,Hubei Provincial Academy of Agricultural science | And 2 more authors.
Animal Science Papers and Reports | Year: 2016

In commercial pig production, litter size has always been one of the most important production traits. Stromal interaction molecule 1 (STIM1) is a transmembrane-spanning Ca2+-binding protein, which is essential for oocyte maturation and fertilization, as well as proper embryo development. The objectives of this study were to identify mutations in the porcine STIM1 gene and investigate the association between genetic variations and litter size traits in pigs. A novel 1bp - deletion/insertion in intron 10 (g.199893-delT) and a single nucleotide polymorphism (SNP) in exon 10 (g.1969037T>C) were found by sequencing. Association results showed that these two mutations significantly affected litter size traits in both the DIV line and Large White pigs (P<0.05). In all parities sows with the TT genotype of g.1969037T>C had significantly more piglets than those of the CC genotype (P<0.01), and the number of piglets decreased in the following order of genotypes TT>CT>CC. The sows with the NN genotype of g.199893-delT had significantly more piglets than those of the MM genotype (P<0.01), and the number of piglets decreased in the following order of genotypes NN>MN>MM. Here we provide evidence that the mutations in the porcine STIM1 gene affected litter size of sows, and that this gene might be a potential important candidate gene related to litter size in pigs. © 2016, Polish Academy of Sciences. All rights reserved.

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