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Guo X.-F.,Wuhan University | Li Y.,Hubei Province Tobacco Quality Supervision and Test Station | Wang H.,Wuhan University | Zhang H.-S.,Wuhan University
Chromatographia | Year: 2014

1,3,5,7-Tetramethyl-8-daminozide-difluoroboradiaza-s-indacence (TMBODIPY-H), a pre-column fluorescent derivatization reagent, was applied to the analysis of fatty acid by high-performance liquid chromatography with fluorescence detection. Using this reagent, 12 fatty acids from propionic acid (C3) to stearic acid (C18) can be derivatized at room temperature in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide within 2 h. The baseline separation of these derivatives can be achieved within 46 min by gradient elution. The detection limits of these fatty acids are in the range of 0.1-1 nM and the linear ranges of most of them are 0.5-100 nM. This was the first application of hydrazine-based difluoro-boraindacene reagent for the analysis of fatty acids, and the proposed method has been successfully used for the determination of saliva samples of smokers and nonsmokers with recoveries of 88-110 %. © 2014 Springer-Verlag. Source


Li Y.,Hubei Province Tobacco Quality Supervision and Test Station | Wang H.,Hubei Province Tobacco Quality Supervision and Test Station | Liao X.,Hubei Province Tobacco Quality Supervision and Test Station | Xiao S.,Hubei Province Tobacco Quality Supervision and Test Station
Tobacco Science and Technology | Year: 2013

A method for determining 18 phthalate compounds in tipping paper was developed with liquid chromatography-tandem mass spectrometry (LC-MS/MS). The samples were ultrasonically extracted with ethanol after soaking with water. Then the extract was analyzed with LC-MS/MS after passing through of an organic phase membrane filter of 0.22 μm. The results showed that: 1) The targeted phthalate compounds could be well separated in 9 minutes with gradient elution by using methanol and 5 mmol/L ammonium acetate in acetic acid-water solution (1:1000, V/V) as the mobile phase at a flow rate of 0.4 mL/min. Multiple reaction monitoring (MRM) mode was adopted for qualitative and quantitative analyses. This method was reliable with the linear range of 1-500 ng/mL and the qualitative detection limit of 0.01-1.85 mg/kg (S/N=3). 2) With the recovery of 82.8%-111.6% and the relative standard deviation (RSD) of less than 10%, this method featured good reproducibility. Source


Li Y.,Hubei Province Tobacco Quality Supervision and Test Station | Wang H.,Hubei Province Tobacco Quality Supervision and Test Station | Liao X.,Hubei Province Tobacco Quality Supervision and Test Station | Xiao S.,Hubei Province Tobacco Quality Supervision and Test Station | Chen H.,Hubei Province Tobacco Quality Supervision and Test Station
Tobacco Science and Technology | Year: 2014

A high performance liquid chromatographic (HPLC) method with a fluorescence detector (FD) was developed for simultaneously determining four prohibited/restricted additives (sesamol, β-naphthol, β-asarone and safrole) in tobacco flavor. Flavor samples were extracted with ethanol by oscillation, then the extract was analyzed with HPLC-FD after passing through an organic phase membrane filter of 0.45 μm. The results showed that: 1) The four additives could be well separated within 8 minutes by gradient elution with acetonitrile and water as mobile phase at a flow rate of 1.0 mL/min. Within the linear range of 0.02-10 μg/mL, the detection limits were from 0.01 to 0.09 mg/kg (S/N=3). The recoveries ranged from 86.4% to 112.1% with the relative standard deviation (RSD) less than 10%. 2) None of the four flavors was detected in the samples of nineteen tobacco flavors by the developed method. This method is simple, rapid and features good reproducibility, it is suitable for the simultaneous determination of sesamol, β-naphthol, β-asarone and safrole in tobacco flavors. ©, 2014, Editorial Office of Tobacco Science and Technology. All right reserved. Source

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