He X.,Huazhong University of Science and Technology |
Liu X.,Huazhong University of Science and Technology |
Liu W.,Huazhong University of Science and Technology |
Wang B.,Huazhong University of Science and Technology |
And 6 more authors.
PLoS ONE | Year: 2015
Background: It is well-recognized that diabetes represents a powerful independent risk factor for cardiovascular diseases. However, very few studies have investigated the relationship between diabetes and risk of aortic dissection (AD). Aim: The aim of this case-control study was to evaluate the association between diabetes and risk of AD in Chinese population. Methods: A hospital-based case-control study, consisting of 2160 AD patients and 4320 controls, was conducted in a Chinese population. Demographic, clinical characteristics and risk factors were collected. Diabetes rate of patients with overall AD, Stanford type A AD and type B AD group was compared with that of corresponding matched control groups. Logistic regression analysis was used to estimate the odds ratios (OR) and 95% confidence intervals (95% CI) for relationship between diabetes and AD risk. Results: The prevalence of diabetes was lower in AD cases than that of control subjects, whether it is the overall AD, type A AD or type B AD group (4.7% vs. 10.0%, 2.9% vs. 8.8%, 5.9% vs. 10.9%, all P<0.001). Furthermore, in multivariate model, diabetes was found to be associated with lower AD risk, which not only applies to the overall AD (OR = 0.2, 95%CI: 0.15-0.26), but also type A AD (OR = 0.12, 95% CI: 0.07-0.20) and type B AD (OR = 0.25, 95% CI: 0.18-0.33). Conclusions: We observed the paradoxical inverse relationship between DM and risk of AD in the Chinese population. These results suggest diabetes may play a protective role in the development of AD. However, further studies are needed to enrich related evidence, especially with regard to underlying mechanisms for these trends. © 2015 He et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Zhu C.-L.,Renmin University of China |
Cheng D.-Z.,Hubei Medical College |
Liu F.,Hubei University |
Yan X.-H.,Hubei University |
And 3 more authors.
Molecular Medicine Reports | Year: 2015
Hepatitis B virus (HBV) infection is one of the major causes of hepatocellular carcinoma (HCC). Kinesin family member 4A (KIF4A) is a microtubule-based motor protein, which is upregulated in cervical and lung cancer. However, the expression of KIF4A in HBV-associated HCC, and the effect of HBV on the expression of KIF4A remain to be elucidated. In the present study, the expression profiles of KIF4A were examined in cancerous tissues and paracancerous tissues from patients with HCC, who presented with histories of chronic HBV infection, and the role of HBV in the induction of the expression of KIF4A was investigated. HepG2 cells were transfected with the pHBV1.3, HBV infectious clone and a construct, which contained the luciferase gene under the control of the KIF4A gene promoter. The results demonstrated that the expression of KIF4A was significantly higher in the HCC tissues than in the paracancerous tissues. HBV activated the KIF4A gene promoter and upregulated the mRNA and protein expression of KIF4A. Furthermore, activation of the gene expression of KIF4A increased in a pHBV1.3 concentration-dependent manner. These results provide novel insights into the understanding of HCC oncogenesis caused by HBV.
Jia X.-T.,Hubei Medical College |
Mu Y.-Y.,Hospital of Women and Children of Oil Field Panjin |
Jia X.-X.,Zunyi Medical College |
Hu W.-H.,Zunyi Medical College |
Deng F.,Zunyi Medical College
Journal of Sichuan University (Medical Science Edition) | Year: 2012
Objective: To investigate the immune reconstitution by the transplantation of human umbilical cord blood CD34 + cells in the NOD/SCID mouse. Methods: Mononuclear cells(MNC) were isolated from human fresh cord blood and CD34 + hematopoietic stem cells were selected by magnetic activated cell sorting method. The selected cells were transplanted via tail vein injection into 16 NOD/SCID mice after sublethal whole-body irradiation. Four mice were sacrificed respectively at 4 th, 6 th, 8 th and 10 th week after the transplantation, the harvested spleen and peripheral blood cells were used to cell phenotype analysis and humoral immune analysis, respectively. There were 14 mice in another two groups, 7 mice did not receive the transplantation after irradiation, 7 were used as blank control (no irradiation, no transplantation). Results: The mice without transplantation all died within 2weeks after irradiation. The survival rate of the mice with transplantation was 37.5% at 6 th week after the irradiation, while the survival rate of blank control was 100%. At 4 th, 6 th, 8 th and 10 th week, the percentage of human CD45 + cells in transplantation group were 4.87 ± 1.23, 9.22 ±2.07, 12.34 ±2.38, 8.14 ±2.36, respectively, and the percentage of CD19 + B lymphocytes were 1.07±0.50, 2.17±0.95, 3.34±0.90,1.67± 0.90, respectively. 10 weeks after the transplantation, human CD19 + B lymphocytes distribution were found in the transplanted mice spleen. Conclusion: The human-mouse chimeric immune model can be built in irradiated NOD/SCID mice by the transplantation of human cord blood CD34 + cells. CD34 + cell differentiation declined with time, which might be due to the lack of appropriate cytokines.
Zhang X.L.,Hubei Medical College
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology | Year: 2011
To investigate the mechanisms of Aldosterone stimulating hepatic stellate cells(HSCs) contraction via Ca2+-independent pathways. HSC-T6 cell line was pre-disposed with Aldo 10mumol/L. The cell contraction was detected by silicone-rubber-membrane cultivation directly. The concentration variation of intracellular free calcium in rat HSC was observed by laser confocal microscopy. Besides, HSC-T6 cell line was under pre-disposal treatment with the blocking agents of Aldo receptor -antisterone, protein kinase C (PKC) special blocking agent-Stauro, Rho kinase blocking agent-Y27632 and MLCK special blocking agent-ML-7 respectively prior to stimulation with aldosterone. RT-PCR was used to detect the expression of Rock2, RhoAGTP and RhoGEF in Ca2+- independent pathways mediated by Rho-kinase. Aldo could induce HSCs contraction. The concentration of intracellular free calcium in rat HSCs had no change after pre-disposal treatment with Aldo. The mRNA expressions of Rock2, RhoAGTP and RhoGEF increased significantly after treatment with Aldo (0.770+/-0.049, 0.960+/-0.096, 0.180+/-0.006, P is less than 0.01).When inhibited with antisterone, the mRNA expressions of the three elements were (0.440+/-0.166, 0.370+/-0.180 and 0.050+/-0.001, P is less than 0.01), lower than that of Aldo group, but higher in ML-7+Stauro + Aldo groups (0.940+/-0.066, 1.330+/-0.192 and 0.160+/-0.007, P is less than 0.05) as compared to the control group (0.140+/-0.023, 0.540+/-0.111 and 0.110+/-0.012). In the Y27632 + ML-7 + Stauro+Aldo group, the mRNA expression of RhoGEF (0.290+/-0.004, P is less than 0.01)was higher than that of the ML-7 + Stauro + Aldo group (0.160+/-0.007). Aldo could induce HSCs contraction via Ca2+-independent pathways and Rho-Rock pathway involved in the process.
Song H.-Y.,Taihe Hospital Affiliated to Hubei Medical College |
He H.-Q.,Hubei Medical College
Journal of Acupuncture and Tuina Science | Year: 2013
Objective: To observe the clinical effects of the surrounding acupuncture for herpes zoster. Methods: Sixty patients with herpes zoster were randomly divided into two groups, a treatment group and a control group, 30 cases in each. The cases in the treatment group were treated with surrounding acupuncture, intravenous infusion of Acyclovir and Ne-Ne laser radiation on the macular areas. While, those in the control group were treated with intravenous infusion of Acyclovir and topical application of Acyclovir ointment. The therapeutic effects were assessed in both groups after two courses of treatment. Results: The treatment group was obviously better than the control group in the effective rate, relief of blister, drying up of blister, relief of pain and time of complete relief of pain (P<0.05). Conclusion: Surrounding acupuncture, combined with intravenous infusion of Acyclovir and He-Ne laser radiation, is remarkable in the therapeutic effects for herpes zoster and worthy of clinical application. © 2013 Shanghai Research Institute of Acupuncture and Meridian and Springer-Verlag Berlin Heidelberg.