Hubei Key Laboratory of Pollutant Analysis and Reuse Technology

Huangshi, China

Hubei Key Laboratory of Pollutant Analysis and Reuse Technology

Huangshi, China
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Wang W.,Hubei Normal University | Wang W.,Hubei Key Laboratory of Pollutant Analysis and Reuse Technology | Chen L.,Hubei Normal University | Lu J.,Hubei Normal University | Hu M.,Wenzhou University
Zhongguo Xitu Xuebao/Journal of the Chinese Rare Earth Society | Year: 2010

In ethanol medium, one solid ternary complex of Eu(III) with 5-fluorouracil-1-yl acetic acid and L-serine was synthesized at 60°C and characterized by elemental analysis, EDTA titration, molar conductance, ultraviolet spectra(UV), infrared spectra(IR), fluorescence spectra, and cyclic voltammetry(CV). The compositions of the complex were confirmed to be [Eu(III)(5-Fu-1-yl)(L-ser)]Cl3·2H2O. The focus was that the electrochemical behavior of ternary complex on the carbon paste working electrode was determined by cyclic voltammetry, and the [Eu(5-Fu-1-yl)(L-ser)]Cl3·2H2O and EuCl3 had electrochemical activity at -0.2~-1.2 V(vs.SCE) in the HAc-NaAc buffer solution (pH≈6.5). The electrochemical active center was Eu3+ in rare earth complex. And there was a good liner relationship between the reduction peak current of complex and concentration and square root of scan rate. The results showed that the system was a standard reversible system.


Wang W.-D.,Wuhan University | Wang W.-D.,Hubei Normal University | Wang J.-S.,Hubei Normal University | Wang J.-S.,Hubei Key Laboratory of Pollutant Analysis and Reuse Technology | And 5 more authors.
International Journal of Biological Macromolecules | Year: 2013

Arginine kinase (AK), a crucial enzyme for the energy metabolism of invertebrates, catalyzes the reversible phosphorylation of arginine by Mg2+ATP to form phosphoarginine and Mg2+ADP. Arginine 330 (R330), not involved in the catalysis of phosphoryl transfer, is a residue highly conserved in the phosphagen kinase family. In order to investigate the role of R330 in AK, it was replaced by lysine (R330K). Non-reduced SDS-PAGE analysis suggested that wild type AK (Wt-AK) and R330K existed in two forms, the reduced form (R-AK or R-R330K) and the oxidized form (O-AK or O-R330K), whereas O-R330K was more susceptible to generate than O-AK. Subsequently, an intramolecular disulfide bond in O-R330K was demonstrated to be formed between Cys201 and Cys271 by site-directed mutagenesis. Biochemical analysis revealed that conformational changes of R330K were concomitant with the sharp decline of catalytic activity. These results were further confirmed by structure modeling of AK and R330K. Therefore, it can be concluded that R330 residue plays an important role in the structural stability and activity of AK. © 2012 Elsevier B.V.


Wang Y.,Hubei Key Laboratory of Pollutant Analysis and Reuse Technology | Wang Y.,Hubei Key Laboratory of Edible Wild Plant Conservation and Utilization | Wang Y.,Hubei Normal University
Czech Journal of Genetics and Plant Breeding | Year: 2014

Many plant genetic engineering taskss require the spatial expression of genes which in turn depends upon the availability of specific promoters. The present paper analyses the green-tissue characteristics of a new L. gibba rbcS promoter driving the expression of the gus gene in transgenic tobacco. A 1491 bp rbcS (small subunit of ribulose bisphosphate carboxylase) promoter was isolated from Lemna gibba. The sequence analysis revealed that this promoter is different from the previously reported rbcS promoter and is named SSU5C. A 1438 bp fragment of the SSU5C promoter was fused with the gus gene and transgenic tobacco plants were generated. The analysis of T1 tobacco p1438-gus revealed that GUS expression driven by the SSU5C promoter was detected in the green part of vegetative organs. The promoter deletion analysis confirmed a region from position –152 to –49 relative to the start of transcription containing boxes X, Y and Z, while a positive regulatory region conferred green tissue-specific expression. Further functional analysis of constructs of box-X, Y, Z, which was fused with the basal SSU5C promoter, confirmed that the boxes X, Y and Z represent the new minimized functional promoter, respectively, and are able to direct green tissue-specific expression. This promoter may be used for gene expression in a tissue-specific manner in plant molecular breeding. © 2014 Czech Academy of Agricultural Sciences. All rights reserved.


Zhang J.,Wuhan University of Science and Technology | Chen H.,Wuhan University of Science and Technology | Chen H.,Hubei Key Laboratory of Pollutant Analysis and Reuse Technology | Chen H.,Fujian Key Laboratory of Polymer Materials | And 3 more authors.
Polymer Bulletin | Year: 2013

Waste rubber powder/polystyrene (WRP/PS) blends with different weight ratio were prepared with styrene grafted styrene butadiene rubber copolymer (PS-g-SBR) as a compatibilizer. The graft copolymer of PS-g-SBR was synthesized by emulsion polymerization method and confirmed through Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC). The copolymer at different weight ratio was subsequently added into the blends. The effects of weight ratio of WRP/PS and compatibilizer loading on mechanical properties were investigated. PS/WRP blends in a weight ratio of 80/20 showed higher impact strength. Moreover, the impact strength of the blend materials increased with the addition of SBR-g-PS, however, decreased at a high loading of the copolymer. The morphology and thermal properties of WRP/PS blends were examined by DSC, scanning electron microscopy (SEM), thermogravimetry (TG). DSC indicated that compared with PS/WRP blend, the glass transition temperature (T g) of PS matrix phase in PS/WRP/SBR-g-PS blend shifted to low temperature because of the formation of chemical crosslinks or boundary layer between PS and WRP, and the T g of WRP phase of both the PS/WRP and PS/WRP/SBR-g-PS blends did not appear. SEM results showed that interfacial adhesion in the blends with the PS-g-SBR copolymer was improved. The morphology was a typical continuous-discontinuous structure. PS and WRP presented continuous phase and discontinuous phase, respectively, indicating the moderate interface adhesion between WRP and PS matrix. TG illustrated that the onset of degradation temperature in the PS/WRP/PS-g-SBR blend decreased slightly by contrast with PS/WRP blend and the degradation of PS/WRP blends with and without SBR-g-PS was completed about at the same values. © 2013 Springer-Verlag Berlin Heidelberg.


Liu N.,Hubei Normal University | Liu N.,Hubei Key Laboratory of Pollutant Analysis and Reuse Technology | Wang J.-S.,Hubei Normal University | Wang J.-S.,Hubei Key Laboratory of Pollutant Analysis and Reuse Technology | And 4 more authors.
International Journal of Biological Macromolecules | Year: 2011

The purpose of this study is to clarify that the amino acid residues (Asp62 and Arg193) are responsible for the activity and stability of arginine kinase (AK). The amino acid residues Asp62 (D62) and Arg193 (R193) are strictly conserved in monomeric AKs and form an ion pair in the transition state analogue complex. In this research, we replaced D62 with glutamate (E) or glycine (G) and R193 with lysine (K) or glycine (G). The mutants of D62E and R193K retained almost 90% of the wild-type activity, whereas D62G and R193G had a pronounced loss in activity. A detailed comparison was made between the physic-chemical properties and conformational changes of wild-type AK and the mutants by means of ultraviolet (UV) difference and fluorescence spectra. The results indicated that the conformation of all of the mutants had been changed and the stability in a urea solution was also reduced. We speculated that the hydrogen bond and electrostatic interactions formed between residues 62 and 193 play a key role in stabilizing the structure and mediating the synergism in substrate binding of arginine kinase from greasyback shrimp (Metapenaeus ensis). © 2011 Elsevier B.V.


Liu N.,Hubei Normal University | Liu N.,Hubei Key Laboratory of Pollutant Analysis and Reuse Technology | Wang J.-S.,Hubei Normal University | Wang J.-S.,Hubei Key Laboratory of Pollutant Analysis and Reuse Technology | And 4 more authors.
International Journal of Biological Macromolecules | Year: 2011

Arginine kinase (AK), a crucial enzyme in energy metabolism, buffers cellular ATP levels by catalyzing the reversible phosphoryl transfer between ATP and arginine. To better understand the role of Cys271 in conformational changes of AK from greasyback shrimp (Metapenaeus ensis), we replaced the residue with serine and alanine. A detailed comparison of the catalytic activity and conformation was made between wild-type AK and the mutants by means of activity analysis, ultraviolet (UV) difference, fluorescence spectrum and size exclusion chromatography (SEC). The results indicated that the catalytic activity of the two mutants was gone. The substrates, arginine-ADP-Mg2+ could induce conformational changes, and additional NO3 - could induce further changes in both the native enzyme and the variants. We speculated that Cys271 might be located in the hinge region between the two domains of AK and cause enzyme conformational changes upon addition of substrate. © 2011 Elsevier B.V.


Sun Y.,Hubei Normal University | Zhou Y.,Hubei Normal University | Liu H.,Hubei Normal University | Liu H.,Hubei Key Laboratory of Pollutant Analysis and Reuse Technology | And 4 more authors.
Journal of Sol-Gel Science and Technology | Year: 2013

Highly preferentially oriented polycrystalline BiFeO3 thin film was grown on FTO/glass substrate by a sol-gel method annealed at 500 C. XRD result showed that the film adopts highly (100) preferential orientation. SEM results indicate that the film is composed of large grains of 40-200 nm and the thickness is about 320 nm. Room temperature saturated polarization and large Pr ~55 μC/cm2 were obtained. Leakage current was substantially reduced by the controlling of ferroelectric polarization. Polarization-modulated conduction mechanism was studied. Moreover, substantial photovoltaic effect was observed. © 2013 Springer Science+Business Media New York.


Wang Y.,Hubei Key Laboratory of Pollutant Analysis and Reuse Technology | Wang Y.,Hubei Normal University
Indian Journal of Genetics and Plant Breeding | Year: 2014

A novel Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit gene (named ssu4d) was cloned from Lemna gibba by a novel chromosome walking technology. The full-length of ssu4d cDNA (named ssu4dc), contained a 522 bp open reading frame encoding a protein of 174 amino acids. Sequence analysis of ssu4dc and ssu4d showed that ssu4d contained an intron between +355 nt to +1125 nt downstream of transcriptional iniative site. ssu4dc contained 54 bp of 5' untranslated region (UTR), and an open reading frame of 174 amino acids consisting of a chloroplast transit peptide with 57 amino acids and a mature protein of 117 amino acids. The deduced amino acid sequence of ssu4dc shared 95-96% identity with L. gibba RbcS protein. Real time-PCR analysis showed differential expression of individual rbcS genes in light-grown Lemna gibba. And the levels of SSU4dc mRNA was regulated by the action of phytochrome, there was variability in the amount of expression of SSU4dc RNA comprared to the SSU1 and SSU5B from Lemna gibba. © 2014, Indian J. Genet. All right reserved.


Sun Y.-X.,Hubei Normal University | Xia Z.,Hubei Normal University | Liu H.-R.,Hubei Normal University | Liu H.-R.,Hubei Key Laboratory of Pollutant Analysis and Reuse Technology | And 3 more authors.
Rengong Jingti Xuebao/Journal of Synthetic Crystals | Year: 2012

BiFeO 3 powders were prepared by the hydrothermal method using Bi(NO 3) 3· 5H 2O and Fe(NO 3) 3· 9H 2O as raw materials.Very even crystallites with a size of 10 μm was obtained, The nanopaticles with 100 nm was obtained by ultrasonic dispersion.SEM results showed that BiFeO 3 nanoparticles are cubic.TEM results indicated that the BiFeO 3 nanoparticles present prefect mono-domain structure. Analyzing on magnetism showed that the BiFeO 3 nanoparticles present weak antiferromagnetic order. Vibration of stretching and Bending were observed and it confirmed the exist of octahedral and the BiFeO 3 has perovskite structure.


Sun Y.,Hubei Normal University | Xiong X.,Hubei Normal University | Xia Z.,Hubei Normal University | Liu H.,Hubei Normal University | And 4 more authors.
Ceramics International | Year: 2013

BiFeO3 and Bi25FeO40 microspheres were synthesized via a hydrothermal process and their structure, visible light response, and magnetism were investigated. Pure BiFeO3 and Bi 25FeO40 powders were obtained at 120 °C and 100 °C, respectively. Both of the Bi25FeO40 and BiFeO 3 powders exhibited strong absorption in visible-light regime. Antiferromagnetism and super-paramagnetism were observed in BiFeO3 and Bi25FeO40 respectively. © 2012 Elsevier Ltd and Techna Group S.r.l.

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