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Sun Y.,Wuhan University | Xie M.,Wuhan University | Huang T.,Wuhan University | Zhang X.,Wuhan University | And 6 more authors.
International Journal of Clinical and Experimental Pathology

Objective: Our previous study demonstrated that α-naphthoflavone (α-NF) inhibits mouse 3T3-L1 pre-ad-ipocytes differentiation via PPARγ, a key transcription factor in adipogenesis. Due to the critical role of inflammation in adipogenesis, we speculated that the suppression role of α-NF in adipogenesis might involve in modulation of cytokines secretion raised by adipocyte differentiation cocktail. Therefore, the present study aims to investigate the role of α-NF in modulating of inflammatory response during adipocytes differentiation and adipocyte-macrophage interaction. Methods: Conditioned medium from different doses of α-NF treated 10-day differentiated 3T3-L1 ad-ipocytes were collected to culture RAW264.7 macrophages. Conditioned medium from activated macrophages and α-NF pre-treated macrophage were used to investigate the effects of α-NF in adipocytes differentiation. Cultured cells and medium were harvested for RT-PCR, Western blot and ELISA. Results: α-NF dose-dependently decreased TNF-α and IL-6 and increased IL-10 expression induced by IDM (Insulin, dexamethasone, isobutylmethylxanthine) in 3T3-L1 pre-adipocytes. Conditioned medium from α-NF treated 3T3-L1 differentiated cells inhibited inflammatory response in mouse macrophage cell line RAW264.7 in contrast to IDM control medium. NFκB activation elicited by IDM was suppressed by α-NF in a dose-response manner. Consequently, decreased TNF-α and increased IL-10 secretion, downstream targets of NFκB signaling pathway, were observed with α-NF in macrophages. Finally, Conditioned medium from α-NF pre-treated, LPS-activated macrophages ameliorated the suppression of 3T3-L1 adipogenesis by LPS activated macrophages. Conclusion: Our results suggest that α-NF regulates inflammation response in both adipocytes and macrophages and adipocyte-macrophage interaction which contributes to pre-adipocyte differentiation. Source

Zhang Y.,Hubei University | Wu W.,Hubei University | Wu W.,Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention | Yu X.,Hubei University | And 2 more authors.
Advance Journal of Food Science and Technology

Ultrasonic-Assisted Extraction (UAE) method was developed for extraction of total flavonoids from Pyracantha fortuneana fruit. The UAE parameters and the antioxidant activities of flavonoids extract were investigated. Important variables and their levels were obtained using single factor analysis method and central composite design. Through Response Surface Methodology (RSM) design experiments, the processing conditions were optimized as follows: ethanol concentration, 81.15%; liquid-solid ratio, 30.00 mL/g; extraction time, 3.14 h; and temperature, 69.55°C. Under the optimum condition, the extraction yield was 1.261%, which was well matched with the predicted values of 1.263%. The antibacterial activities of extract against four bacterial strains were determined by agar well diffusion method and the results indicated that the extract showed potent antibacterial activities, which concludes its application as natural antibacterial agent. © Maxwell Scientific Organization, 2014. Source

Zheng R.-P.,Wuhan University | Zheng R.-P.,Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention | Bai T.,Wuhan University | Zhou X.-G.,Wuhan University | And 5 more authors.
Molecular Medicine Reports

This study aimed to examine the effects of Lefty A protein on transforming growth factor-β1 (TGF-β1)-mediated apoptosis in human renal tubular epithelial cells (HK-2). HK-2 cells were transfected with the human Lefty gene to induce the secretion of endogenous Lefty A protein. Following exposure of the HK-2 cells to recombinant human TGF-β1 (10 ng/ml), p-Smad2/3 protein levels were examined by western blot analysis, and cellular apoptosis was detected by flow cytometry 6, 12, 24 and 48 h following TGF-β1 treatment. Coculture of renal tubular epithelial cells with TGF-β1 resulted in a significant increase in p-Smad2/3 protein levels and the rate of cell apoptosis, which were attenuated by liposome-mediated transfection with the Lefty gene. Lefty A protein was able to inhibit the TGF-β1/Smad signaling pathway and markedly attenuate TGF-β1-mediated apoptosis in human renal tubular epithelial cells. Taken together, these results indicated that the TGF-β1/Smad signaling pathway most likely mediates apoptosis in renal tubular epithelial cells. In addition, Lefty A protein is capable of inhibiting the TGF-β1/Smad pathway to reduce TGF-β1/Smad-mediated apoptosis in renal tubular epithelial cells. This study may provide novel insights into the prevention and treatment of urinary tract obstruction disease using Lefty A protein. Source

Wu Y.,Central University of Costa Rica | Wu Y.,Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention | Zhang K.,Central University of Costa Rica | Zhang K.,Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention | And 8 more authors.
Journal of International Medical Research

Objective: To investigate the relationship between the serum concentration of high mobility group box 1 protein (HMGB1) and oxidative stress in patients with atrial fibrillation (AF). Methods: Patients with AF (paroxysmal or persistent) and matched control subjects were recruited. Serum HMGB1 concentration and malondialdehyde (MDA) and superoxide dismutase (SOD) activity were determined. Results: Serum hs-CRP and HMGB1 concentrations and MDA activity were significantly higher in patients with persistent AF (n=33) or paroxysmal AF (n=53) than in controls (n=30). Serum SOD activity was significantly lower in both patient groups than in controls. In the patient group, HMGB1 concentration was significantly positively correlated with MDA activity (r=0.535), and negatively correlated with SOD activity (r=-0.491). MDA, SOD, hs-CRP and HMGB1 were significant independent predictors of AF. Conclusions: Increased oxidative stress may contribute to increased HMGB1 concentrations in patients with AF. Inhibition of oxidative stress may provide a potential therapeutic strategy for AF. © The Author(s) 2013. Source

Zhang Y.,Hubei University | Zhang L.,Hubei University | Zhang G.,Hubei University | Li S.,Hubei University | And 16 more authors.
Tumor Biology

Osteosarcoma is the most commonly diagnosed primary malignant bone tumor, with similar global incidence rate across childhood and adolescence. Patients with localized disease have a 5-year survival period of 80 %; however, the prognosis is poor in those with metastatic osteosarcoma. The origin of the primary tumor is most frequently the metaphyseal (actively growing) regions of the distal femur, proximal tibia, and proximal humerus, although the tumor can develop in any bone, and the most likely sites for metastasis are the lungs and bone. Ezrin is a member of the ezrin-radixin-moesin (ERM) family of proteins that functions as a cross-linker between the actin cytoskeleton and the plasma membrane, and ezrin also plays a positive role in maintaining cell shape and polarity and facilitates membrane-trafficking pathways, cell migration, cell signaling, growth regulation, and differentiation. There is strong evidence to suggest that ezrin is necessary for osteosarcoma metastasis. The objective of the current review is to summarize the know-how about metastatic progression in osteosarcoma, with a focus on ezrin. Despite the promise that preliminary studies on ezrin have shown, there is a great need to further analyze the role of ezrin in osteosarcoma metastasis and to determine its usefulness as a biomarker for the disease. © 2014 International Society of Oncology and BioMarkers (ISOBM). Source

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