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Qin D.,Hubei Academy of Agricultural Science | Qin D.,Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement | Dong J.,Hubei Academy of Agricultural Science | Dong J.,Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement | And 10 more authors.
BMC Genomics | Year: 2015

Background: Leaf color variations are common in plants. Herein we describe a natural mutant of barley cultivar Edamai No.6, whs18, whose leaf color showed stable and inheritable stage-green-revertible-albino under field condition. Methods: Bulked Segregant Analysis (BSA) based on SSR assay and Specific Length Amplified Fragment Sequencing (SLAF-seq) was used to map the candidate gene for this trait. Results: We found that leaf color of whs18 was green at seedling stage, while the seventh or eighth leaf began to show etiolation, and albino leaves emerged after a short period. The newly emerged leaves began to show stripe white before jointing stage, and normal green leaves emerged gradually. The duration of whs18 with abnormal leaf color lasted for about 3 months, which had some negative impacts on yield-related-traits. Further investigations showed that the variation was associated with changes in chlorophyII content and chloroplast development. Genetic analysis revealed that the trait was controlled by a single recessive nuclear gene, and was designed as HvSGRA in this study. Based on the F2 population derived from Edamai No.9706 and whs18, we initially mapped the HvSGRA gene on the short arm of chromosome 2H using SSR and BSA. GBMS247 on 2HS showed co-segregation with HvSGRA. The genetic distance between the other marker GBM1187 and HvSGRA was 1.2 cM. Further analysis using BSA with SLAF-seq also identified this region as candidate region. Finally, HvSGRA interval was narrowed to 0.4 cM between morex_contig_160447 and morex_contig_92239, which were anchored to two adjacent FP contigs, contig_34437 and contig_46434, respectively. Furthermore, six putative genes with high-confidence in this interval were identified by POPSEQ. Further analysis showed that the substitution from C to A in the third exon of fructokinase-1-like gene generated a premature stop codon in whs18, which may lead to loss function of this gene. Conclusions: Using SSR and SLAF-seq in conjunction with BSA, we mapped HvSGRA within two adjacent FP contigs of barley. The mutation of fructokinase-1-like gene in whs18 may cause the stage green-revertible albino of barley. The current study lays foundation for hierarchical map-based cloning of HvSGRA and utilizing the gene/trait as a visualized maker in molecular breeding in future. © 2015 Qin et al.

Jiang Y.,Huazhong Agricultural University | Meng J.,Huazhong Agricultural University | Zhang L.,Huazhong Agricultural University | Cai M.,Huazhong Agricultural University | And 8 more authors.
Field Crops Research | Year: 2014

Using a transgenic Bacillus thuringiensis (Bt) crop is reportedly one of the most effective ways to improve insect resistance; however, the insertion of external Bt genes may affect the non-target growth characteristics of crop. Field experiments were conducted to investigate the yield performance and physiological traits in an elite restorer rice line, Minghui63 (MH63) with Cry1C*, Cry2A* or Cry1Ab/Ac genes under three fertilizer applications [zero nitrogen fertilizer (N0), zero potassium fertilizer (K0) and recommended nitrogen and potassium fertilizer (NK)]. In comparison with MH63, MH63 (Cry1C*) experienced declining grain yields of 15.3-31.0% under different fertilizer applications because of the decreased setting rates associated with the poorer matter transport-related traits. The grain yield of MH63 (Cry2A*) was averagely 18.4% lower than MH63 in only the N0 treatment due to the accelerated leaf senescence, which was correlated with the higher ratios of the Bt protein content to the soluble protein content (BTC/SPC) in the MH63 (Cry2A*) leaves at the filling stage (FS). The yield performance of MH63 (Cry1Ab/Ac) was identical to MH63. In addition, no significant differences were recorded between Bt-MH63 and MH63 regarding N and K assimilation. Hence, our results suggest that the Cry1C* and Cry2A* transgenes affect the yield performance and related traits of MH63 in response to nitrogen and/or potassium applications. © 2014 Elsevier B.V.

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