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Wu Y.,Northwest University, China | Wu Y.,Hubei Academy of Agricultural Science | Wu Y.,Hubei Key Laboratory of Animal Embryo and Molecular Breeding | Liu X.,Northwest University, China | And 3 more authors.
Molecular Biology Reports | Year: 2011

Adipose differentiation-related protein (ADRP) is a protein which induced during the early onset of the adipose differentiation program and is expressed at high level in mature adipocytes. In the present study, 1311 bp of ADRP in Peking duck (Anas platyrhynchos) was cloned by reverse transcription (RT)-PCR. The cloned sequence of ADRP was highly conserved, although relevant changes with respect to human, mouse and chicken were identified. Real-time quantitative PCR was used to determine if the ADRP was expressed in different tissues of male and female Peking ducks. The expression of ADRP was widely distributed in all examined tissues. The expression level of it was highest in abdominal fat and lowest (only few) in lung of male and female Peking ducks. © Springer Science+Business Media B.V. 2010. Source


Wu Y.,Hubei Academy of Agricultural Science | Wu Y.,Hubei Key Laboratory of Animal Embryo and Molecular Breeding | Pi J.S.,Hubei Academy of Agricultural Science | Pan A.L.,Hubei Academy of Agricultural Science | And 5 more authors.
Biochemical Genetics | Year: 2012

Myogenic differentiation 1 (MyoD1) genes belong to the MyoD gene family and play key roles in growth and muscle development. This study was designed to investigate the effects of variants in the MyoD1 gene on duck growth and carcass traits. Three duck populations (Cherry Valley, Jingjiang, and Muscovy) were sampled, their growth and carcass traits were measured, and they were genotyped using the PCR-RFLP method. The results showed one novel polymorphism, an alteration in intron 2 of the MyoD1 gene (A to T). It was associated with the traits of weight at 8 weeks, carcass weight, breast muscle weight, leg muscle weight, eviscerated percentage, percentage of leg muscle weight, dressing percentage, and lean meat percentage. This alteration in intron 2 of MyoD1 may be linked with potential major loci or genes affecting some growth and carcass traits. © Springer Science+Business Media, LLC 2012. Source


Wu Y.,Hubei Academy of Agricultural Science | Wu Y.,Hubei Key Laboratory of Animal Embryo and Molecular Breeding | Pi J.S.,Hubei Academy of Agricultural Science | Pan A.L.,Hubei Academy of Agricultural Science | And 4 more authors.
Archiv Tierzucht | Year: 2015

The very low density lipoprotein receptor (VLDLR) is an important multifunctional receptor and plays a key role in chicken reproduction. This study is designed to investigate the effect of variants in the VLDLR gene on quail laying traits. Two quail populations were studied - yellow feather quail and chestnut feather quail; 384 individuals per breed were used. The laying traits (the weight of the first egg, the age of the first egg, egg weight, the weight of 20-week-old and the egg number of 20-week-old) were measured and recorded. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to genotype those individuals. The results showed two novel polymorphisms, i.e. two linkage variations in intron 11 of the VLDLR gene (363T > C and 392C > T). They are associated with the weight of the first egg, the age of the first egg and egg number of 20-week-old in yellow feather and chestnut feather quail. The two variations in intron 11 of VLDLR may be linked with potential major loci or genes affecting some laying traits. © Author(s) 2015. CC Attribution 3.0 License. Source


Wu Y.,Hubei Academy of Agricultural Science | Wu Y.,Hubei Key Laboratory of Animal Embryo and Molecular Breeding | Xiao H.W.,Hubei Academy of Agricultural Science | Liang Z.H.,Hubei Academy of Agricultural Science | And 6 more authors.
Czech Journal of Animal Science | Year: 2014

In the present study, quantitative real-time PCR was employed to investigate expression profiling and expression difference of ESR1 and ESR2 in ovaries of Shaoxing duck and Jingjiang duck during three laying stages. Results showed the expression levels of ESR1 and ESR2 in ovaries were increased from the age of the first egg to the age of 500 days in both duck breeds. The expression of ESR1 in Shaoxing duck was lower than that in Jingjiang duck for the age of the first egg and of 180 days, and for the age of 500 days it was higher in Shaoxing duck than in Jingjiang duck. The ESR2 showed converse expression profiling in the two duck breeds. The results suggest that ESR1 and ESR2 mediate the process of egg laying in ducks, and that ESR2 may play a more important role for the ovary during egg-laying stages and may be closely related to the laying performance of the ducks. Source


Wen G.,Hubei Academy of Agricultural Science | Wen G.,Hubei Key Laboratory of Animal Embryo and Molecular Breeding | Shang Y.,Huazhong Agricultural University | Guo J.,Huazhong Agricultural University | And 6 more authors.
Virus Genes | Year: 2013

A thermostable avirulent Newcastle disease virus (NDV) strain TS09-C was developed by serial passage of V4 strain in BHK-21 cells. The complete genome sequence of strain TS09-C was determined and compared with the sequences of other NDV isolates representing different thermostable phenotypes. The TS09-C genome was 15,186 nucleotides long and consisted of eight genes in the order of 3′-NP-P/V/W-M-F-HN-L-5′. High levels of nucleotide and amino acid sequence identity existed among the thermostable NDV isolates. Thermostable strains TS09-C, V4, I-2, and Ulster all belonged to genotype I. The F protein cleavage site of strain TS09-C was 112G-K-Q-R-R-L117, with an isolated basic amino acid and a pair of contiguous basic amino acids, differing from that of its parental V4 strain (112G-K-Q-G-R-L 117). Our characterization of the complete genome of thermostable avirulent NDV strain TS09-C may facilitate the development of a thermostable NDV reverse genetic system and further study of the mechanism of thermostability of NDV. © 2013 Springer Science+Business Media New York. Source

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