Hubei Entry Exit Inspection and Quarantine Bureau

Wuhan, China

Hubei Entry Exit Inspection and Quarantine Bureau

Wuhan, China
SEARCH FILTERS
Time filter
Source Type

Feng Y.,Yangtze University | Feng H.-L.,Hubei Entry exit Inspection and Quarantine Bureau | Fang Y.-H.,Gezhouba Senior High School | Su Y.-B.,Yangtze University
Experimental Parasitology | Year: 2017

Khawia sinensis is an important species in freshwater fish causing considerable economic losses to the breeding industry. This is the first mt genome of a caryophyllidean cestode characterised. The entire mt genome of K. sinensis is 13,759 bp in length. This mt genome contains 12 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and two non-coding regions. The arrangement of the K. sinensis mt genome is the same as other tapeworms, however, the incomplete stop codon (A) is more frequent that other species. Phylogenetic analyses based on concatenated amino-acid sequences of the 12 protein-coding genes of 17 tapeworms including K. sinensis were conducted to assess the relationship of K. sinensis with other species, the result indicated K. sinensis was closely related with cestode species. This complete mt genome of K. sinensis will enrich the mitochondrial genome databases of tapeworms and provide important molecular markers for ecology, diagnostics, population variation and evolution of K. sinensis and other species. © 2017 Elsevier Inc.


Tang J.,Hubei University | Xu Z.,Hubei University | Zhou L.,Hubei University | Qin H.,Hubei Entry Exit Inspection and Quarantine Bureau | And 2 more authors.
Diagnostic Microbiology and Infectious Disease | Year: 2010

Based on gold-labeled silver stain (GLSS) method, we developed the visual protein microarray for simultaneous, sensitive, and specific detection of Ureaplasma parvum and Chlamydia trachomatis using N-terminus multiple-banded antigen (NMBA) of U. parvum and major outer membrane protein of C. trachomatis. The specific antigens were immobilized on glass surface that was treated with 3-glycidoxypropyltrimethoxysilane, and they were used as the capturing probes to recognize the complementary target antibodies binding to the detecting probes of Nano-gold-Staphylococcal protein A (SPA). In the "sandwich" format, Nano-gold-SPA probe was used as an indicator and GLSS was applied to amplify the detection signals and produce black image on array spots, which were visible with naked eyes. In our model arrays, the detection limit of protein microarray was as low as 2 ng/mL, and the lowest titer of detectable antibody was 1:128; thus, this sensitivity was comparable to the fluorescent detection method. The visual simultaneous protein microarrays were used to detect total 186 clinical samples, which had been determined by enzyme-linked immunosorbent assay (ELISA) and fluorescence quantitative real-time polymerase chain reaction; the results were identical and no distinct difference (P > 0.05) existed between them. Our results demonstrate that we have developed the visual protein microarray technique, which is of high sensitivity and high specificity, and it may have potential in clinical applications. © 2010 Elsevier Inc.


Fang R.,Huazhong Agricultural University | Feng H.,Hubei Entry exit Inspection and Quarantine Bureau | Nie H.,Huazhong Agricultural University | Wang L.,Huazhong Agricultural University | And 4 more authors.
Vaccine | Year: 2010

Toxoplasma gondii is a protozoan parasite causing toxoplasmosis to almost one-third of population all over the world. One of the most efficient ways to control this disease is immunization. However, so far, there is no effective vaccine available against this pathogen. Recently, a baculovirus pseudotype with vesicular stomatitis virus G protein (Bac-VSV-G) was found to efficiently transduce and express transgenes on mammalian cells, so it was considered as an excellent expressing vector. In this study, the value of Bac-VSV-G in delivering T. gondii antigen was investigated. T. gondii SAG1 gene was cloned into Bac-VSV-G, and recombinant baculovirus BV-G-SAG1 was obtained. Indirect immunofluorescence test showed BV-G-SAG1 was efficiently transduced and expressed in pig kidney cells. Then BALB/c mice were immunized with BV-G-SAG1 at different doses (1 × 108, 1 × 109, and 1 × 1010 PFU/mouse) and challenged with T. gondii RH strain tachyzoites after immunization. The levels of specific T. gondii antibody, interferon (IFN)-γ, IL-4, IL-10 expression and release, and the survival rate of treated mice were evaluated. Compared with the mice immunized with DNA vaccine (pcDNA/SAG1) encoding the same gene, BV-G-SAG1 induced higher levels of specific T. gondii antibody and (IFN)-γ expression with dose-dependent manner and the survival rate of mice with BV-G-SAG1 was significantly improved. These results indicated that pseudotype baculovirus-mediated gene delivery can be utilized as an alternative strategy to develop new generation of vaccines against T. gondii infection. © 2009 Elsevier Ltd. All rights reserved.


Yang X.,Huazhong Agricultural University | Zhao Y.,Huazhong Agricultural University | Wang L.,Hubei Provincial Center for Diseases Control and Prevention | Feng H.,Hubei Entry Exit Inspection and Quarantine Bureau | And 5 more authors.
Parasites and Vectors | Year: 2015

Background: Fischoederius elongates is an important trematode of Paramphistomes in ruminants. Animals infected with F. elongates often don't show obvious symptoms, so it is easy to be ignored. However it can cause severe economic losses to the breeding industry. Knowledge of the mitochondrial genome of F. elongates can be used for phylogenetic and epidemiological studies. Findings: The complete mt genome sequence of F. elongates is 14,120 bp in length and contains 12 protein-coding genes, 22 tRNA genes, two rRNA genes and two non-coding regions (LNR and SNR). The gene arrangement of F. elongates is the same as other trematodes, such as Fasciola hepatica and Paramphistomum cervi. Phylogenetic analyses using concatenated amino acid sequences of the 12 protein-coding genes by Maximum-likelihood and Neighbor-joining analysis method showed that F. elongates was closely related to P. cervi. Conclusion: The complete mt genome sequence of F. elongates should provide information for phylogenetic and epidemiological studies for F. elongates and the family Paramphistomidae. © 2015 Yang et al.; licensee BioMed Central.


Yang X.,Huazhong Agricultural University | Gasser R.B.,University of Melbourne | Koehler A.V.,University of Melbourne | Wang L.,Hubei Provincial Center for Diseases Control and Prevention | And 5 more authors.
Parasites and Vectors | Year: 2015

Background: Hypoderaeum conoideum is a neglected but important trematode. The life cycle of this parasite is complex: snails serve as the first intermediate hosts: bivalves, fishes or tadpoles serve as the second intermediate hosts, and poultry (such as chickens and ducks) act as definitive hosts. In recent years, H. conoideum has caused significant economic losses to the poultry industry in some Asian countries. Despite its importance, little is known about the molecular ecology and population genetics of this parasite. Knowledge of mitochondrial (mt) genome of H. conoideum can provide a foundation for phylogenetic studies as well as epidemiological investigations. Methods: The entire mt genome of H. conoideum was amplified in five overlapping fragments by PCR and sequenced, annotated and compared with mt genomes of selected trematodes. A phylogenetic analysis of concatenated mt amino acid sequence data for H. conoideum, eight other digeneans (Clonorchis sinensis, Fasciola gigantica, F. hepatica, Opisthorchis felineus, Schistosoma haematobium, S. japonicum, S. mekongi and S. spindale) and one tapeworm (Taenia solium; outgroup) was conducted to assess their relationships. Results: The complete mt genome of H. conoideum is 14,180 bp in length, and contains 12 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and one non-coding region (NCR). The gene arrangement is the same as in Fasciola spp, with all genes being transcribed in the same direction. The phylogenetic analysis showed that H. conoideum had a relatively close relationship with F. hepatica and other members of the Fasciolidae, followed by the Opisthorchiidae, and then the Schistosomatidae. Conclusions: The mt genome of H. conoideum should be useful as a resource for comparative mt genomic studies of trematodes and for DNA markers for systematic, population genetic and epidemiological studies of H. conoideum and congeners. © 2015 Yang et al.; licensee BioMed Central.


Sun J.-X.,Huazhong University of Science and Technology | Zhao X.-Y.,Hubei Entry Exit Inspection and Quarantine Bureau | Fu X.-F.,Hubei Entry Exit Inspection and Quarantine Bureau | Yu H.-Y.,Huazhong University of Science and Technology | And 3 more authors.
Chemical and Pharmaceutical Bulletin | Year: 2012

Phytochemical investigations of the root bark of Juglans cathayensis DODE. led to the isolation of three new naphthalenyl glycosides, Jugnaphthalenoside A-C (1-3). Their structures were elucidated on the basis of extensive analysis of spectroscopic data. The cytotoxicities of the three new compounds were also evaluated. © 2012 The Pharmaceutical Society of Japan.


Zhang J.-G.,Beijing Institute of Technology | Wang K.,Beijing Institute of Technology | Li Z.-M.,Beijing Institute of Technology | Zheng H.,Hubei Entry Exit Inspection and Quarantine Bureau | And 2 more authors.
Main Group Chemistry | Year: 2011

A novel energetic cesium compound based on trinitroresorein, [Cs 2(HTNR)(OH)(H2O)]n (H2TNR: 2,4,6-trinitroresorcinol) has been synthesized by the reaction of cesium nitrate and styphnic acid. The molecular structure was characterized by using X-ray diffraction analysis, elemental analysis and FT-IR analysis. The crystalline material is monoclinic with space group P2(1)/c and the empirical formula is Cs2C6H5N3O10. The unit cell parameters are: a = 6.843(1) , b = 10.185(2) , c = 19.026(4) , β = 96.21(1)°, V = 1318.21(40) 3, Z = 4, Dc = 2.746 Mg/m3, Mr = 544.95, F(000) = 1008, μ(Mo K α) = 5.588 mm-1. The thermal decomposition mechanism of the compound was studied by differential scanning calorimetry (DSC) and thermo-gravimetric derivative (TG-DTG) techniques. At the linear heating rate of 10°C/min, the thermal decomposition of the compound finally evolved CsCN and other gas products, which indicated three decomposition processes between 60 and 500°C. © 2011-IOS Press and the authors. All rights reserved.


PubMed | Yangtze University, Huazhong Agricultural University, Hubei Provincial Center for Diseases Control and Prevention, Wuhan University of Technology and Hubei Entry exit Inspection and Quarantine Bureau
Type: Journal Article | Journal: Parasitology research | Year: 2016

Clinostomum complanatum is an important trematode in fishes, birds of the family Ardeidae, and humans. Until now, limited knowledge is available regarding its molecular epidemiology, ecology, and phylogenetic study. Knowledge of the full mitochondrial genome of C. complanatum will provide important information for the study of epidemiology, biology, and genetic diversity of this fluke. In the present study, the complete mitochondrial genome of C. complanatum was sequenced and analyzed. The complete C. complanatum mitochondrial genome is 13,796bp in length and contains 12 protein-coding genes, 22 tRNA genes, two rRNA genes, and one non-coding control region. All the 12 protein-coding genes are transcribed in the same direction and are AT-rich. Phylogenetic analysis based on concatenated amino acid sequences of the 12 protein-coding genes from C. complanatum and other selective digeneans showed that C. complanatum is in a separate branch, indicating C. complanatum has no closer relationship with any of the selected families. The complete mtDNA sequence of C. complanatum will increase our knowledge of mitochondrial genomics data and will also provide an important resource for studies of inter- and intra-species variation of flukes belonging to Clinostomidae.


PubMed | Huazhong Agricultural University, Hubei Provincial Center for Diseases Control and Prevention and Hubei Entry Exit Inspection and Quarantine Bureau
Type: Journal Article | Journal: Parasitology research | Year: 2016

Gastrodiscidae species are neglected but significant paramphistomes in small ruminants, which can lead to considerable economic losses to the breeding industry of livestock. However, knowledge about molecular ecology, population genetics, and phylogenetic analysis is still limited. In the present study, we firstly sequenced and analyzed the full mitochondrial (mt) genome of Homalogaster paloniae (14,490bp). The gene contents and organization of the H. paloniae mt genome is the same as that of other digeneans, such as Fasciola hepatica and Paramphistomum cervi. It is interesting that unlike other paramphistomes, H. paloniae is flat in shape which is similar with Fasciola, such as F. hepatica. Phylogenetic analysis of H. paloniae and other 17 selected digeneans using concatenated amino acid sequences of the 12 protein-coding genes showed that Gastrodiscidae is closely related to Paramphistomidae and Gastrothylacidae. The availability of the mt genome sequence of H. paloniae should provide an important foundation for further molecular study of Gastrodiscidae and other digeneans.


PubMed | Huazhong Agricultural University, Hubei Provincial Center for Diseases Control and Prevention, University of Melbourne and Hubei Entry Exit Inspection and Quarantine Bureau
Type: | Journal: Parasites & vectors | Year: 2015

Hypoderaeum conoideum is a neglected but important trematode. The life cycle of this parasite is complex: snails serve as the first intermediate hosts: bivalves, fishes or tadpoles serve as the second intermediate hosts, and poultry (such as chickens and ducks) act as definitive hosts. In recent years, H. conoideum has caused significant economic losses to the poultry industry in some Asian countries. Despite its importance, little is known about the molecular ecology and population genetics of this parasite. Knowledge of mitochondrial (mt) genome of H. conoideum can provide a foundation for phylogenetic studies as well as epidemiological investigations.The entire mt genome of H. conoideum was amplified in five overlapping fragments by PCR and sequenced, annotated and compared with mt genomes of selected trematodes. A phylogenetic analysis of concatenated mt amino acid sequence data for H. conoideum, eight other digeneans (Clonorchis sinensis, Fasciola gigantica, F. hepatica, Opisthorchis felineus, Schistosoma haematobium, S. japonicum, S. mekongi and S. spindale) and one tapeworm (Taenia solium; outgroup) was conducted to assess their relationships.The complete mt genome of H. conoideum is 14,180bp in length, and contains 12 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and one non-coding region (NCR). The gene arrangement is the same as in Fasciola spp, with all genes being transcribed in the same direction. The phylogenetic analysis showed that H. conoideum had a relatively close relationship with F. hepatica and other members of the Fasciolidae, followed by the Opisthorchiidae, and then the Schistosomatidae.The mt genome of H. conoideum should be useful as a resource for comparative mt genomic studies of trematodes and for DNA markers for systematic, population genetic and epidemiological studies of H. conoideum and congeners.

Loading Hubei Entry Exit Inspection and Quarantine Bureau collaborators
Loading Hubei Entry Exit Inspection and Quarantine Bureau collaborators