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Zhang X.,University of Hertfordshire | Zhang X.,Chongqing Normal University | Zhang X.,Southwest University | White R.P.,Rothamsted Research | And 12 more authors.
Plant Pathology | Year: 2014

In China, the incidence of phoma stem canker observed in pre-harvest surveys from 2005 to 2012 was greater on winter oilseed rape in provinces in central China (in May) than on spring oilseed rape in north China (in August). In all 742 cases when the causal pathogen was isolated from stem cankers, it was identified as Leptosphaeria biglobosa by morphology in culture and/or by species-specific polymerase chain reaction. Both L. biglobosa and Leptosphaeria maculans were detected on crop debris and seed in shipments of oilseed rape seed imported into China through Shanghai or Wuhan ports in 2009-2011. Descriptions of the observed spread of L. maculans into areas previously colonized by L. biglobosa across a spring oilseed rape growing region (Alberta, Canada, westwards, 1984-1998) and across a winter oilseed rape growing region (Poland, eastwards, 1984-2004) were used to estimate the potential westward spread of L. maculans in China across spring oilseed rape growing regions (north China) and winter oilseed rape growing regions (central China, generally provinces along the Yangtze River), respectively. The rates of spread were estimated as 47 km per year across spring oilseed rape in north China and 70 km per year across winter oilseed rape in central China. Dispersal modelling suggested that the rate of spread of L. maculans across Alberta, Canada (c. 17 km per year) could be explained by windborne dispersal of ascospores. © 2013 British Society for Plant Pathology.

Fang R.,Huazhong Agricultural University | Feng H.,Hubei Entry Exit Inspection and Quarantine Bureau | Nie H.,Huazhong Agricultural University | Wang L.,Huazhong Agricultural University | And 4 more authors.
Vaccine | Year: 2010

Toxoplasma gondii is a protozoan parasite causing toxoplasmosis to almost one-third of population all over the world. One of the most efficient ways to control this disease is immunization. However, so far, there is no effective vaccine available against this pathogen. Recently, a baculovirus pseudotype with vesicular stomatitis virus G protein (Bac-VSV-G) was found to efficiently transduce and express transgenes on mammalian cells, so it was considered as an excellent expressing vector. In this study, the value of Bac-VSV-G in delivering T. gondii antigen was investigated. T. gondii SAG1 gene was cloned into Bac-VSV-G, and recombinant baculovirus BV-G-SAG1 was obtained. Indirect immunofluorescence test showed BV-G-SAG1 was efficiently transduced and expressed in pig kidney cells. Then BALB/c mice were immunized with BV-G-SAG1 at different doses (1 × 108, 1 × 109, and 1 × 1010 PFU/mouse) and challenged with T. gondii RH strain tachyzoites after immunization. The levels of specific T. gondii antibody, interferon (IFN)-γ, IL-4, IL-10 expression and release, and the survival rate of treated mice were evaluated. Compared with the mice immunized with DNA vaccine (pcDNA/SAG1) encoding the same gene, BV-G-SAG1 induced higher levels of specific T. gondii antibody and (IFN)-γ expression with dose-dependent manner and the survival rate of mice with BV-G-SAG1 was significantly improved. These results indicated that pseudotype baculovirus-mediated gene delivery can be utilized as an alternative strategy to develop new generation of vaccines against T. gondii infection. © 2009 Elsevier Ltd. All rights reserved.

Zhou L.,Hubei University of Medicine | Zhou L.,China University of Technology | Gong R.,Hubei Entry Exit Inspection and Quarantine Bureau | Lu X.,Hubei University of Medicine | And 3 more authors.
Japanese Journal of Infectious Diseases | Year: 2015

Treponema pallidum, hepatitis C virus (HCV), human immunodeficiency virus (HIV)-1, and hepatitis B virus (HBV) are major causes of sexually transmitted diseases passed through blood contact. The development of a sensitive and efficient method for detection is critical for early diagnosis and for large-scale screening of blood specimens in China. This study aims to establish an assay to detect these pathogens in clinical serum specimens. We established a TaqMan-locked nucleic acid (LNA) real-time polymerase chain reaction (PCR) assay for rapid, sensitive, specific, quantitative, and simultaneous detection and identification. The copy numbers of standards of these 4 pathogens were quantified. Standard curves were generated by determining the mean cycle threshold values versus 10-fold serial di-lutions of standards over a range of 106 to 101 copies/µL, with the lowest detection limit of the assay being 101 copies/µL. The assay was applied to 328 clinical specimens and compared with enzyme-linked immunosorbent assay (ELISA) and commercial nucleic acid testing (NAT) methods. The assay identified 39 T. pallidum-, 96 HCV-, 13 HIV-1-, 123 HBV-, 5 HBV/HCV-, 1 T. pallidum/HBV-, 1 HIV-1/HCV-, and 1 HIV-1/T. pallidum-positive specimens. The high sensitivity of the assay confers strong potential for its use as a highly reliable, cost-effective, and useful molecular diagnostic tool for large-scale screening of clinical specimens. This assay will assist in the study of the pathogenesis and epidemiology of sexually transmitted blood diseases. © 2015, National Institute of Health. All rights reserved.

Yang X.,Huazhong Agricultural University | Zhao Y.,Huazhong Agricultural University | Wang L.,Hubei Provincial Center for Diseases Control and Prevention | Feng H.,Hubei Entry Exit Inspection and Quarantine Bureau | And 5 more authors.
Parasites and Vectors | Year: 2015

Background: Fischoederius elongates is an important trematode of Paramphistomes in ruminants. Animals infected with F. elongates often don't show obvious symptoms, so it is easy to be ignored. However it can cause severe economic losses to the breeding industry. Knowledge of the mitochondrial genome of F. elongates can be used for phylogenetic and epidemiological studies. Findings: The complete mt genome sequence of F. elongates is 14,120 bp in length and contains 12 protein-coding genes, 22 tRNA genes, two rRNA genes and two non-coding regions (LNR and SNR). The gene arrangement of F. elongates is the same as other trematodes, such as Fasciola hepatica and Paramphistomum cervi. Phylogenetic analyses using concatenated amino acid sequences of the 12 protein-coding genes by Maximum-likelihood and Neighbor-joining analysis method showed that F. elongates was closely related to P. cervi. Conclusion: The complete mt genome sequence of F. elongates should provide information for phylogenetic and epidemiological studies for F. elongates and the family Paramphistomidae. © 2015 Yang et al.; licensee BioMed Central.

Chen L.,Huazhong Agricultural University | Feng Y.,Yangtze University | Chen H.-M.,Wuhan University of Technology | Wang L.-X.,Hubei Provincial Center for Diseases Control and Prevention | And 4 more authors.
Parasitology Research | Year: 2016

Clinostomum complanatum is an important trematode in fishes, birds of the family Ardeidae, and humans. Until now, limited knowledge is available regarding its molecular epidemiology, ecology, and phylogenetic study. Knowledge of the full mitochondrial genome of C. complanatum will provide important information for the study of epidemiology, biology, and genetic diversity of this fluke. In the present study, the complete mitochondrial genome of C. complanatum was sequenced and analyzed. The complete C. complanatum mitochondrial genome is 13,796 bp in length and contains 12 protein-coding genes, 22 tRNA genes, two rRNA genes, and one non-coding control region. All the 12 protein-coding genes are transcribed in the same direction and are AT-rich. Phylogenetic analysis based on concatenated amino acid sequences of the 12 protein-coding genes from C. complanatum and other selective digeneans showed that C. complanatum is in a separate branch, indicating C. complanatum has no closer relationship with any of the selected families. The complete mtDNA sequence of C. complanatum will increase our knowledge of mitochondrial genomics data and will also provide an important resource for studies of inter- and intra-species variation of flukes belonging to Clinostomidae. © 2016 Springer-Verlag Berlin Heidelberg

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