Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains

Huanggang, China

Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains

Huanggang, China
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Wang S.,Huanggang Normal University | Wang S.,Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains | Zhou G.,Wuhan Institute of Technology | Huang X.,Wuhan Institute of Technology | And 6 more authors.
Euphytica | Year: 2017

Non-heading Chinese cabbage (Brassica campestris L. ssp. chinensis Makino), an important vegetable in East Asian countries, is rich in vitamin C, dietary fiber, and other health-enhancing factors. As a staple vegetable, its genetic studies have been hindered due to the lack of polymorphic genetic markers. In this research, 2.3 and 2.1 Gb RNA-seq data have been obtained for a heat-sensitive cultivar “GHA” and a heat-resistant cultivar “XK”, respectively. Totally, 3267 novel transcripts were identified from the assembled 29,037 transcripts. A total of 139 differential expressed genes (DEGs) have been identified. Most DEGs were involved in the signal transduction and general function prediction. Moreover, 37 polymorphic EST-SSR loci and 285,573 SNP markers have been developed. This study will provide valuable transcript sequences and functional markers for further genetic diversity analysis, quantitative trait locus identification, as well as molecular marker assisted selection of Brassicaceae species. © 2017, Springer Science+Business Media Dordrecht.

Yang G.-L.,Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains | Yang G.-L.,Huanggang Normal University | Li S.-M.,Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains | Li S.-M.,Huanggang Normal University | And 3 more authors.
Toxin Reviews | Year: 2016

Ribosome-inactivating proteins (RIPs) are a class of toxin proteins capable of acting on 28S rRNA of eukaryotic cells and inhibiting protein biosynthesis. Multiple properties have been reported for RIPs, including RNA N-glycosidase activity, polynucleotide: adenosine glycosidase activity, DNase-like activity and RNA hydrolase activity among others. RIPs isolated from bitter melon have shown strong biological activities of anti-virus, anti-bacteria, anti-fungi, apoptosis of tumor cells and early abortion induction. Ribosome inactivation and nuclease activity were firstly introduced in this paper, followed by a detailed discussion of RIP biological activity, and then the immunogenicity and immunotoxicity of RIPs. In this review, we aim to summarize pharmacological properties of RIPs purified from Mormordica charantia, the construction of effective immunotoxins for clinic usage as well as tumor therapy in the future. © 2016 Informa UK Limited, trading as Taylor & Francis Group.

Xie F.,Jiangxi Academy of Agricultural science | Xie F.,Huazhong Agricultural University | Fan R.,Huazhong Agricultural University | Yi Q.,Huazhong Agricultural University | And 5 more authors.
Hydrometallurgy | Year: 2016

A low cost bio-sorbent, named "PPF resin", was prepared by immobilizing persimmon residual with formaldehyde and it was characterized before and after adsorption of Pd(II). The adsorption behavior of PPF resin towards Pd(II) from aqueous solutions was studied by batch and column adsorption methods. The adsorption equilibrium of Pd(II) on PPF resin was achieved within 840min, and the experimental data were well fitted with the pseudo-second-order rate model. Higher initial concentration and adsorption temperature led to higher equilibrium adsorption amount, and the adsorption isotherms could be well described by Langmuir model. The column studies suggested that the resin was effective for the adsorption of Pd(II) from aqueous solutions, and the loaded Pd(II) could be easily desorbed by acidic thiourea solution. The adsorption process of Pd(II) on PPF resin might be the multiple reactions of electrostatic interaction and reductive adsorption, in which Pd(II) was reduced to Pd(0) as confirmed by the XRD pattern of PPF resin after adsorption of Pd(II). The resin exhibited outstanding selective adsorption towards Pd(II) from actual leach liquor of waste PCBs at a high concentration of HNO3. The results suggested that the PPF resin can be used as an active bio-sorbent for the recovery of Pd(II) from aqueous environment. © 2016 Elsevier B.V.

Guan C.,Huazhong Agricultural University | Chen W.,Huazhong Agricultural University | Mo R.,Huazhong Agricultural University | Du X.,Jiangxi Academy of Agricultural science | And 4 more authors.
Frontiers in Plant Science | Year: 2016

Chinese pollination-constant non-astringent (C-PCNA) persimmon (Diospyros kaki Thunb.) is considered to be an important germplasm resource for the breeding of PCNA cultivars, though its molecular mechanisms of astringency removal remain to be elucidated. Previously, we showed that the abundance of pyruvate kinase gene transcripts increased rapidly during astringency removal in C-PCNA persimmon fruit. Here, we report the full-length coding sequences of six novel DkPK genes from C-PCNA persimmon fruit isolated based on a complementary DNA (cDNA) library and transcriptome data. The expression patterns of these six DkPK genes and correlations with the soluble proanthocyanidin (PA) content were analyzed during various fruit development stages in different types of persimmon, with DkPK1 showing an expression pattern during the last stage in C-PCNA persimmon that was positively correlated with a decrease in soluble PAs. Phylogenetic analysis revealed that DkPK1 belongs to cytosolic-1 subgroup, and subcellular localization analysis confirmed that DkPK1 is located in the cytosol. Notably, tissue expression profiling revealed ubiquitous DkPK1 expression in different persimmon organs, with the highest expression in seeds. Furthermore, transient over-expression of DkPK1 in persimmon leaves resulted in a significant decrease in the content of soluble PAs but a significant increase in the transcript levels of pyruvate decarboxylase genes (DkPDC1, -3, -4, -5), which catalyze the conversion of pyruvate to acetaldehyde. Thus, we propose that an acetaldehyde-based coagulation effect reduces the content of soluble PAs. Taken together, our results suggest that DkPK1 might be involved in the natural removal of astringency at the last developmental stage in C-PCNA persimmon. This is the first report to identify several novel full-length DkPK genes as well as their potential roles in the natural loss of astringency in C-PCNA persimmon. © 2016, Guan, Chen, Mo, Du, Zhang and Luo.

Yi Q.,Huazhong Agricultural University | Yi Q.,Jingchu University of Technology | Fan R.,Huazhong Agricultural University | Xie F.,Jiangxi Academy of Agricultural science | And 4 more authors.
Journal of the Taiwan Institute of Chemical Engineers | Year: 2016

A natural resin, named "PPF resin", was prepared by cross-linking persimmon tannin with formaldehyde and employed as adsorbents for selective recovery of Pd(II) from nitric acid medium. Parameters such as acidic media, HNO3 concentration, contact time, initial metal concentration and temperature were investigated in detail. The adsorption behavior followed the typical monolayer type of Langmuir model and the maximum adsorption capacity reached 259.7mgg-1 at 323K. The obtained thermodynamic parameters (δG, δH and δS) revealed that this adsorption process was feasible, spontaneous and endothermic process. After adequately characterizing the samples by FT-IR, XRD, XPS and SEM-EDS, the adsorption mechanism was proposed to be complex formation and redox reaction. The Pd(II) recovery from industrial nitric acid extract of printed circuit boards was effective and highly selective, and the loaded Pd(II) can be easily desorbed using acidic thiourea solutions. Therefore, the present results provide a new, low cost and effective approach for palladium recovery from e-wastes in nitric acid medium. © 2016 Taiwan Institute of Chemical Engineers.

Sheng P.,Huazhong Agricultural University | Sheng P.,Jiangxi Academy of science | Sheng P.,Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains | Li Y.,Huazhong Agricultural University | And 4 more authors.
International Journal of Molecular Sciences | Year: 2015

In this study, we used a culture-independent method based on library construction and sequencing to analyze the genetic diversity of the cellulase and hemicellulase genes of the bacterial community resident in the hindgut of Holotrichia parallela larvae. The results indicate that there is a large, diverse set of bacterial genes encoding lignocellulose hydrolysis enzymes in the hindgut of H. parallela. The total of 101 distinct gene fragments (similarity <95%) of glycosyl hydrolase families including GH2 (24 genes), GH8 (27 genes), GH10 (19 genes), GH11 (14 genes) and GH36 (17 genes) families was retrieved, and certain sequences of GH2 (10.61%), GH8 (3.33%), and GH11 (18.42%) families had <60% identities with known sequences in GenBank, indicating their novelty. Based on phylogenetic analysis, sequences from hemicellulase families were related to enzymes from Bacteroidetes and Firmicutes. Fragments from cellulase family were most associated with the phylum of Proteobacteria. Furthermore, a full-length endo-xylanase gene was obtained, and the enzyme exhibited activity over a broad range of pH levels. Our results indicate that there are large number of cellulolytic and xylanolytic bacteria in the hindgut of H. parallela larvae, and these symbiotic bacteria play an important role in the degradation of roots and other organic matter for the host insect. © 2015 by the authors; licensee MDPI, Basel, Switzerland.

PubMed | Fudan University, Pennsylvania State University, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, CAS Kunming Institute of Botany and Smithsonian Institution
Type: | Journal: Molecular biology and evolution | Year: 2016

Fruits are the defining feature of angiosperms, likely have contributed to angiosperm successes by protecting and dispersing seeds, and provide foods to humans and other animals, with many morphological types and important ecological and agricultural implications. Rosaceae is a family with ~3000 species and an extraordinary spectrum of distinct fruits, including fleshy peach, apple, and strawberry prized by their consumers, as well as dry achenetum and follicetum with features facilitating seed dispersal, excellent for studying fruit evolution. To address Rosaceae fruit evolution and other questions, we generated 125 new transcriptome and genomic datasets and identified hundreds of nuclear genes to reconstruct a well-resolved Rosaceae phylogeny with highly supported monophyly of all subfamilies and tribes. Molecular clock analysis revealed an estimated age of ~101.6 Mya for crown Rosaceae and divergence times of tribes and genera, providing a geological and climate context for fruit evolution. Phylogenomic analysis yielded strong evidence for numerous whole genome duplications (WGDs), supporting the hypothesis that the apple tribe had a WGD and revealing another one shared by fleshy fruit-bearing members of this tribe, with moderate support for WGDs in the peach tribe and other groups. Ancestral character reconstruction for fruit types supports independent origins of fleshy fruits from dry-fruit ancestors, including the evolution of drupes (e.g., peach) and pomes (e.g., apple) from follicetum, and drupetum (raspberry and blackberry) from achenetum. We propose that WGDs and environmental factors, including animals, contributed to the evolution of the many fruits in Rosaceae, which provide a foundation for understanding fruit evolution.

Yang S.,Huazhong Agricultural University | Jiang Y.,Huazhong Agricultural University | Xu L.,Huazhong Agricultural University | Shiratake K.,Nagoya University | And 4 more authors.
Plant Physiology and Biochemistry | Year: 2016

Persimmon fruits accumulate a large amount of proanthocyanidins (PAs) in “tannin cells” during development that cause the sensation of astringency due to coagulation of oral proteins. Pollination-constant non-astringent (PCNA) is a spontaneous mutant persimmon phenotype that loses its astringency naturally on the tree at maturity; while the more common non-PCNA fruits remain rich in PAs until they are fully ripened. Here, we isolated a DkMATE1 gene encoding a Multidrug And Toxic Compound Extrusion (MATE) family protein from the Chinese PCNA (C-PCNA) ‘Eshi 1’. Expression patterns of DkMATE1 were positively correlated with the accumulation of PAs in different types of persimmons fruits during fruit development. An analysis of the inferred amino acid sequences and phylogenetic relationships indicated that DkMATE1 is a putative PA precursor transporter, and subcellular localization assays revealed that DkMATE1 is localized in the vacuolar membrane. Ectopic expression of the DkMATE1 in Arabidopsis tt12 mutant supported that DkMATE1 could complement its biological function in transporting epicatechin 3′-O-glucoside as a PAs precursor from the cytoplasm to vacuole. Furthermore, the transient over-expression and silencing of DkMATE1 in ‘Mopanshi’ persimmon leaves resulted in a significant increase and a decrease in PA content, respectively. The analysis of cis-elements in DkMATE1 promoter regions indicated that DkMATE1 might be regulated by DkMYB4, another well-known structural gene in persimmon. Overall, our results show that DkMATE1 may be an essential PA precursor membrane transporter that plays an important role in PA biosynthesis in persimmon. © 2016 Elsevier Masson SAS

Raboanatahiry N.H.,Huazhong University of Science and Technology | Raboanatahiry N.H.,Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains | Yin Y.,Huazhong University of Science and Technology | Yin Y.,Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains | And 4 more authors.
BMC Genomics | Year: 2015

Acyl-coA binding proteins (ACBPs) bind long chain acyl-CoA esters with very high affinity. Their possible involvement in fatty acid transportation from the plastid to the endoplasmic reticulum, prior to the formation of triacylglycerol has been suggested. Four classes of ACBPs were identified in Arabidopsis thaliana: the small ACBPs, the large ACBPs, the ankyrin repeats containing ACBPs and the kelch motif containing ACBPs. They differed in structure and in size, and showed multiple important functions. In the present study, Brassica napus ACBPs were identified and characterized. Results: Eight copies of kelch motif ACBPs were cloned, it showed that B. napus ACBPs shared high amino acid sequence identity with A. thaliana, Brassica rapa and Brassica oleracea. Furthermore, phylogeny based on domain structure and comparison map showed the relationship and the evolution of ACBPs within Brassicaceae family: ACBPs evolved into four separate classes with different structure. Chromosome locations comparison showed conserved syntenic blocks. Conclusions: ACBPs were highly conserved in Brassicaceae. They evolved from a common ancestor, but domain duplication and rearrangement might separate them into four distinct classes, with different structure and functions. Otherwise, B. napus inherited kelch motif ACBPs from ancestor conserving chromosomal location, emphasizing preserved synteny block region. This study provided a first insight for exploring ACBPs in B. napus, which supplies a valuable tool for crop improvement in agriculture. © 2015 Raboanatahiry et al.

Mo R.,Huazhong Agricultural University | Yang S.,Huazhong Agricultural University | Huang Y.,Huazhong Agricultural University | Chen W.,Huazhong Agricultural University | And 4 more authors.
Tree Genetics and Genomes | Year: 2016

Pollination constant non-astringency (PCNA)-type persimmons are the most desirable cultivar because the fruit loses astringency naturally and does not require any treatments for edibility. The mechanism of natural astringency loss in Chinese PCNA (C-PCNA)-type persimmon is probably related to the coagulation of soluble tannins into insoluble tannins, which is quite different from that in the Japanese PCNA (J-PCNA) type. In this work, three types of persimmon cultivars were sampled: ‘Luotian-tianshi’ (C-PCNA), ‘Maekawa-jirou’ (J-PCNA), and ‘Mopanshi’ (pollination constant astringent (PCA)) were sampled. Three DkADH and four DkPDC genes were isolated from C-PCNA plants. Three candidate genes for soluble tannins coagulation identified in C-PCNA fruit (DkADH1, DkPDC1, and DkPDC2) were characterized through combined analysis of spatiotemporal expression patterns and tannin and acetaldehyde contents during fruit development. Transient over-expression in persimmon leaves showed that DkADH1 and DkPDC2 led to a significant decrease in the levels of soluble tannins in infiltrated leaves. These results indicated that DkADH and DkPDC genes should be considered key genes for natural astringency loss in C-PCNA types. © 2016, Springer-Verlag Berlin Heidelberg.

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