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He J.-B.,Key Laboratory of Pesticide and Chemical Biology CCNU | He J.-B.,Wuhan Polytechnic University | He J.-B.,Hubei Collaborative Innovation Center for Processing of Agricultural Products | He H.-F.,Key Laboratory of Pesticide and Chemical Biology CCNU | And 6 more authors.
Bioorganic and Medicinal Chemistry | Year: 2015

To identify new antifungal lead compound based on inhibitors of pyruvate dehydrogenase complex E1, a series of 5-iodo-1,4-disubstituted-1,2,3-triazole derivatives 3 were prepared and evaluated for their Escherichia coli PDHc-E1 inhibitory activity and antifungal activity. The in vitro bioassay for the PDHc-E1 inhibition indicated all the compounds exhibited significant inhibition against E. coli PDHc-E1 (IC50 <21 μM), special compound 3g showed the most potent inhibitory activity (IC50 = 4.21 ± 0.11 μM) and was demonstrated to act as a competitive inhibitor of PDHc-E1. Meanwhile, inhibitor 3g exhibited very good enzyme-selective inhibition of PDHc-E1 between pig heart and E. coli. The assay of antifungal activity showed compounds 3e, 3g, and 3n exhibited fair to good activity against Rhizoctonia solani and Botrytis cinerea even at 12.5 μg/mL. Especially compound 3n (EC50 = 5.4 μg/mL; EC90 = 21.1 μg/mL) exhibited almost 5.50 times inhibitory potency against B. cinerea than that of pyrimethanil (EC50 = 29.6 μg/mL; EC90 = 113.4 μg/mL). Therefore, in this study, compound 3n was found to be a novel lead compound for further optimization to find more potent antifungal compounds as microbial PDHc-E1 inhibitors. © 2015 Elsevier Ltd. All rights reserved. Source

Oliveira H.,Wuhan Polytechnic University | Oliveira H.,University of Porto | Wu N.,Wuhan Polytechnic University | Zhang Q.,Wuhan Polytechnic University | And 7 more authors.
Food and Function | Year: 2016

In this study, the gastric transport efficiency of malvidin-3-glucoside and several derivatives was assayed on the MKN-28 cell model. The transport efficiency was found to increase for all compounds with the incubation time. Pyranoanthocyanins may slightly impair transport efficiency levels in comparison with native anthocyanins. Among the pyranoanthocyanin derivatives the presence of the carbonyl group and the absence of charge were important for the transport efficiency percentage of oxovitisin and apparently compensated the negative effect associated with the additional ring. Moreover, the antiproliferative properties of these compounds in the MCF-7 cancer cell line were assayed, oxovitisin being the most effective compound in inhibiting the proliferation of MCF-7 cells. Also, a kinetic incorporation of oxovitisin was assayed revealing that this pyranoanthocyanin is quickly incorporated into cells. This study confirms the importance of the natural micro-oxidative processes that occur during the ageing of anthocyanin-containing food and their impact on their bioavailability and bioactivity properties. © 2016 The Royal Society of Chemistry. Source

Liu S.,Wuhan Polytechnic University | Chen J.,Wuhan Polytechnic University | Chen J.,Hubei Collaborative Innovation Center for Processing of Agricultural Products | Chen L.,Wuhan Polytechnic University | Gao J.,Wuhan Polytechnic University
Journal of the Chinese Cereals and Oils Association | Year: 2015

Physicochemical properties and morphological structure of rice dreg protein (RDP), rice dreg decarboxamidation protein (RDDP) and rice dreg foaming protein (RDFP) had been investigated. Solubility of RDP was low and stable at pH 2~12, solubility of RDDP decreased at pH 2~4.5 then increased at pH 4.5~12 and solubility of RDFP was more than 90%. Compared to RDP, foaming force of RDDP and RDFP increased of more than 39% and 126% at pH 8~ 10 respectively and reached the maximum 56% and 196% at pH 9.5 respectively. Emulsibility of RDP was stable and emulsibility of RDDP firstly decreased and then increased, while that of RDFP increased. Total amount of essential amino acid of RDDP and RDFP decreased 8.29% and 7.7% respectively and the hydrophobicity increased 9.14% and 30% respectively. There was some glycoprotein and secondary structure for -helix and-strand in RDP, RDDP, and RDFP, meanwhile the quantity decreased successively. RDP presented closely as aggregate ball, RDDP became much smaller clumpy aggregations, and RDFP became a large piece of layer structure. The results indicated that RDFP might be used as a promising protein foam powder ingredient in food industry for its excellent physicochemical properties. ©, 2015, Journal of the Chinese Cereals and Oils Association. All right reserved. Source

Yi Y.,Wuhan Polytechnic University | Yi Y.,Hubei Collaborative Innovation Center for Processing of Agricultural Products | Sun J.,Wuhan Polytechnic University | Wang L.-M.,Wuhan Polytechnic University | And 2 more authors.
Modern Food Science and Technology | Year: 2015

Longan is a popular fruit and is widely distributed throughout the subtropical area. The dried pulp of longan has been used in traditional Chinese medicine and is made up of polysaccharides as one of the main bioactive ingredients. The nutritional difference between fresh and dried longan pulp products was investigated using analytical methods and the physicochemical properties and immunomodulatory activity of polysaccharides were evaluated. Gas chromatography-mass spectrometry (GC-MS), ion exchange chromatography, gel filtration chromatography, viscometry, ultraviolet (UV) spectroscopy, and infrared (IR) spectroscopy were performed. The results showed that the longan polysaccharides from fresh and dried fruits (i.e., LPF and LPD, respectively) were polysaccharide-protein complexes, but significant differences were found in their binding protein contents, neutral/acid polysaccharide ratios, monosaccharide compositions, molecular weight distributions, and intrinsic viscosities. Cellular in vitro tests confirmed LPD strongly stimulated lymphocyte proliferation and macrophage phagocytosis compared to LPF in the dose range of 50~400 µg/mL. Both polysaccharides significantly stimulated ConA-induced lymphocyte proliferation and nitric oxide production from macrophages (P<0.05), but had a weak effect on lipopolysaccharide (LPS)-induced lymphocyte proliferation. The drying process of longan pulp may enhance the in vitro immunomodulatory activity of water-soluble polysaccharides by changing their physicochemical characteristics. © 2015, South China University of Technology. All right reserved. Source

Chen J.,Wuhan Polytechnic University | Chen J.,Hubei Collaborative Innovation Center for Processing of Agricultural Products | Chen Y.,Wuhan Polytechnic University | Xia W.,Wuhan Polytechnic University | And 6 more authors.
International Journal of Food Science and Technology | Year: 2016

In this study, grass carp peptides were prepared by enzymatic hydrolysis of grass carp protein using the combination of Alcalase and Neutrase, and angiotensin-I converting enzyme (ACE) inhibitory activity in vitro, antihypertensive activity in vivo, antioxidant activities, and physicochemical properties of peptides achieved from grass carp protein were characterised after ultrafiltration and desalted processes using mixed ion exchange resins. The purified peptides exhibited strong ACE inhibitory activity (IC50= 105 μg mL-1), antihypertensive activity with the maximal drop for systolic blood pressure (SBP) of 43 mmHg at a dosage of 100 mg per kg body weight in spontaneously hypertensive rat (SHR), and antioxidant activities indicated by thiobarbituric acid-reactive substance values in a liposome-oxidising system, radical-scavenging activity and chelation of metal ions (Fe2+). The molecular weight of peptides was <1000 Da. Compared to grass carp protein, the peptides separated from enzymatic hydrolysates possessed similar amino acid compositions, but contained higher concentrations of essential amino acids. Moreover, the peptides exhibited excellent solubility at a wide range of pH values from 2 to 10, and lower apparent viscosity than the protein. The peptides separated from enzymatic hydrolysates might be used as a promising ingredient in antihypertensive functional foods and nutraceuticals. Changes in systolic blood pressure 6 (SBP) by administering the purified grass carp peptides (50 mg kg-1 body weight and 100 mg kg-1 body weight) in SHR. © 2016 Institute of Food Science and Technology. Source

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