Hubei Collaborative Innovation Center for Green Transformation of Bio Resources

Wuhan, China

Hubei Collaborative Innovation Center for Green Transformation of Bio Resources

Wuhan, China
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Dai C.,Hubei University | Dai C.,Hubei Collaborative Innovation Center for Green Transformation of Bio Resources | Dai C.,Hubei Provincial Key Laboratory of Regional Development and Environmental Response | Liang X.,Hubei University | And 4 more authors.
Annals of botany | Year: 2016

KEY RESULTS: We found greater variation in style deflexion within flowers and plants than among plants. Variation of style deflexion at the flower and plant level was positively correlated, suggesting that variability in style deflexion may be a distinct trait in P. incarnata. Lower deflexion and reduced variation in that deflexion increased pollen deposition, which in turn increased seed number. However, lower styles also increased self-pollen deposition. In contrast, higher deflexion and greater variability of that deflexion increased variation in pollen deposition, which resulted in heavier seeds.CONCLUSIONS: Variability of style deflexion and therefore stigma placement, independent from the mean, appears to be a property of individual P. incarnata plants. The mean and variability of style deflexion in P. incarnata affected seed number and seed weight in contrasting ways, through the quantity and potentially quality of pollen deposition. This antagonistic selection via different fitness components may maintain diverse style phenotypes.BACKGROUND AND AIMS: Floral traits are essential for ensuring successful pollination and reproduction in flowering plants. In particular, style and anther positions are key for pollination accuracy and efficiency. Variation in these traits among individuals has been well studied, but less is known about variation within flowers and plants and its effect on pollination and reproductive success.METHODS: Style deflexion is responsible for herkogamy and important for pollen deposition in Passiflora incarnata. The degree of deflexion may vary among stigmas within flowers as well as among flowers. We measured the variability of style deflexion at both the flower and the plant level. The fitness consequences of the mean and variation of style deflexion were then evaluated under natural pollination by determining their relationship to pollen deposition, seed production and average seed weight using structural equation modelling. In addition, the relationship between style deflexion and self-pollen deposition was estimated in a greenhouse experiment. © The Author 2016. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oup.com.


Dai C.,Hubei University | Dai C.,Hubei Collaborative Innovation Center for Green Transformation of Bio Resources | Dai C.,Hubei Key Laboratory of Regional Development and Environmental Response | Luo W.-J.,Hubei University | And 10 more authors.
Pakistan Journal of Botany | Year: 2017

Allelopathy is an important ecological mechanism in nature, which is extensively investigated and acknowledged as an innate characteristic. Less is known how the direction and strength of allelopathy may change with different environments in living plants. We studied the allelopathic potential of Oxalis rubra on seedlings of Veronica persica, a cosmopolitan weed, planted under two environments (light incubator and laboratory) with fundamental difference in light intensity. In the light incubator, O. rubra showed a moderate level of inhibition on the leaf growth of V. persica, probably due to a higher concentration or faster release of allelochemicals owing to high resource availability. On the contrary, the presence of O. rubra played a significant role in promoting the survival of V. persica seedlings in the laboratory, likely ascribed to water retention by O. rubra plants or stimulatory allelopathy in the different environment. Our study contributes to empirical investigations on how allelopathy varies with different conditions and points out the importance of environmental heterogeneity on allelopathy. © 2017, Pakistan Botanical Society. All rights reserved.


Zhang J.,Hubei University | Zhang J.,Hubei Collaborative Innovation Center for Green Transformation of Bio Resources | Li Z.H.,Hubei University | Zhou P.Q.,Huazhong University of Science and Technology | And 3 more authors.
International Journal of Environmental Technology and Management | Year: 2015

During the past ten years (2000-2009), a continuous survey has been conducted to assess the concentration of trace metals in the water from three parts of Daye Lake. The results indicate that: 1) concentration of three trace metals exceed the Chinese drinking water standard measures; out of these As exceeded significantly by 70% followed by Pb and Cd which are 66.67% and 13.34% respectively; 2) all the water in Daye Lake had been polluted by trace metals and had high potential health risk as a drinking water resource for human beings; among the three research areas of Daye Lake: Sanli Qi Lake had the highest health risk (58.8 × 10-4), followed by Yinjia Lake (24.12 × 10-4), while Main Lake had the lowest health risks (3.84 × 10-4); 3) no significant correlations between these trace metals was observed (p < 0.5) which suggest they originated from diverse pollution sources. Copyright © 2015 Inderscience Enterprises Ltd.


Zhang L.,Chinese Academy of Agricultural Sciences | Zhang L.,Laboratory of Risk Assessment for Oilseeds Products Wuhan | Zhang L.,Quality Inspection and Test Center for Oilseeds Products | Zhang L.,Hubei Collaborative Innovation Center for Green Transformation of Bio Resources | And 18 more authors.
Chemometrics and Intelligent Laboratory Systems | Year: 2016

Highly predictive multivariate calibration model depends on samples in training set. In this study, we introduced an outlier detection method and developed its improvement for shorter run time. Improved Monte-Carlo outlier detection (IMCOD) was proposed to establish cross-prediction models for determining normal samples, which were subsequently used to analyze the distribution of prediction errors for all of dubious samples together. Four real datasets were employed to illustrate and validate the performance of IMCOD. After sample selection for training set of NIR of soy flour samples, the Root Mean Square Error of Prediction (RMSEP) of PLS model decreased from 1.4811 to 0.7650. This method benefits the establishment of a good model for QSAR and NIR datasets. © 2015 Elsevier B.V.


Zhang L.,Chinese Academy of Agricultural Sciences | Zhang L.,Laboratory of Risk Assessment for Oilseeds Products Wuhan | Zhang L.,Quality Inspection and Test Center for Oilseeds Products | Zhang L.,Hubei Collaborative Innovation Center for Green Transformation of Bio Resources | And 17 more authors.
RSC Advances | Year: 2015

Developing a method of identifying oil authenticity is becoming critical for protecting customers' rights as adulteration of edible oils is a particular concern in food quality. Since adulterants in edible oils are usually unknown, the authenticity identification is a one-class classification problem in chemometrics. In this study, a one-class classification model was built to identify the authenticity of peanut oils by fatty acid profiles. Based on previous studies, 28 fatty acids were identified and quantified for peanut oils. The authenticity identification model was built by one-class partial least squares (OCPLS) classifier for peanut oils. Subsequently, the established model was validated by independent test sets. The results indicated that the OCPLS classifier could effectively detect adulterated oils and was therefore employed for authenticity assessment. Moreover, counterfeit oils adulterated with different levels of other edible oils were simulated by the Monte Carlo method and employed to test the lowest adulteration level of this one-class classifier. As a result, the model could identify peanut oils and sensitively detect adulteration of edible oils with other vegetable oils at adulteration level of more than 4%. © 2015 The Royal Society of Chemistry.


Li L.-J.,University of Jinan | Li L.-J.,Hubei Collaborative Innovation Center for Green Transformation of Bio Resources | Yu L.-J.,Hubei Collaborative Innovation Center for Green Transformation of Bio Resources | Wu Z.-Z.,University of Jinan | Liu X.,Huazhong University of Science and Technology
Chinese Traditional and Herbal Drugs | Year: 2015

Objective: To investigate the chemical constituents from the aerial part of Rabdosia flexicaulis. Methods: Various column chromatography techniques were used to isolate and purify the compounds and their structures were identified by the spectral data. Results: Fifteen compounds were isolated and identified as rotundic acid (1), tormentic acid (2), corsolic acid (3), 23-hydroxyursolic acid (4), maslinic acid (5), teuclatriol (6), 3,6-dihydroxy-1-menthen (7), 1- hydroxypinoresinol (8), epipinoresinol (9), lariciresinol (10), caffeic acid (11), rosmarinic acid (12), 3'-O-methyl-rosmarinic acid (13), methyl 4'-O-methyl-rosmarinate (14), and methyl 3-dehydroxyl-rosmarinate (15). Conclusion: All the compounds are isolated from the species for the first time, among which compounds 1, 6, 7, 9, 13, and 14 are isolated from the plants in genus Rabdosia (Bl.) Hassk. for the first time. ©, 2015, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.


Zhang J.,Hubei University | Zhang J.,Hubei Collaborative Innovation Center for Green Transformation of Bio Resources | Manske G.,University of Bonn | Zhou P.Q.,Huazhong University of Science and Technology | And 3 more authors.
Environment, Development and Sustainability | Year: 2016

Overuse of nitrogen (N) fertilizers in agriculture activities has caused severe water pollution in China. The lack of data at producer level hampers decision makers in the development and implementation of efficient policies to curb excessive N-fertilizer use. In a survey of 300 farm households in the Liangzihu Lake basin, we identified factors associated with farmers’ decisions on N-fertilizer use and application rate. Household survey and multiple linear regression models indicate that the average application rate in the study region is 229 kg N ha−1, which exceeds the recommended rate for maximum profit for cereal crops (maize, wheat, and rice) in China of 150–180 kg N ha−1. High N-application rates are associated with low farmland productivity (coefficient = −15.66, p = 0.02), a high share of off-farm income (coefficient = 27.14, p = 0.003), and a low education level of the household head (coefficient = −10.83, p = 0.039). Neither physical infrastructure nor access to input markets appears to be related to N-application rates. It may be concluded that excessive use of N in agriculture of Central China is mainly a problem of insufficient awareness and high share of off-farm income. © 2016 Springer Science+Business Media Dordrecht


Yu X.,Hubei University | Yu X.,Hubei Key Laboratory of Industrial Biotechnology | Yu X.,Hubei Collaborative Innovation Center for Green Transformation of Bio resources | Wang X.,Hubei University | And 13 more authors.
Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology | Year: 2014

To express recombinant carboxypeptidase from Thermus aquaticus (Cpase Taq) in Pichia pastosis, the open reading frame coding thermostable Cpase Taq was optimized based on the preference of P. pastoris codon usage and synthesized in vitro. The novel gene was cloned into P. pastoris expression vector pHBM905A and the sequence coding 6×His tag was fused with the ORF of Cpase Taq gene. The recombinant plasmid was named pHBM905A-Cpase Taq and transformed into P. pastoris GS115. Transformants were induced with 1% methanol for 72 h until the enzyme yield reached 0.1 mg/ml. The enzyme was purified and its enzymatic properties were analyzed. The results showed that the specific enzyme activity reached maximum at 75℃ and pH 7.5|, which was about 80 U/mg. It was the first report about the secretory expression of Cpase Taq in P. pastoris GS115. Because of its large-scale preparation, this enzyme may be applied in industrial hydrolysis of peptides into amino acids in the future. © 2014 Chin J Biotech, All rights reserved.


Wu R.,Chinese Academy of Agricultural Sciences | Wu R.,Key Laboratory of Biology and Genetic Improvement of Oil Crops | Wu R.,Key Laboratory of Detection for Mycotoxins | Wu R.,Laboratory of Risk Assessment for Oilseeds Products Wuhan | And 30 more authors.
Food Chemistry | Year: 2016

A novel magnetic carboxylated multi-walled carbon nanotubes (c-MWCNT-MNPs) was proposed for magnetic solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry to determine phenolic compounds in sesame oil. In this study, c-MWCNT-MNPs were acquired by simply dispersing Fe3O4 magnetic nanoparticles into carboxylated multi-walled carbon nanotubes. The major parameters affecting extraction efficiency were optimized, including the type and volume of desorption solvents, extraction and desorption time, washing solution, and sorbent amount. The limit of quantifications and limit of detections were from 0.03 μg/kg to 43.00 μg/kg and from 0.01 μg/kg to 13.60 μg/kg, respectively. The recoveries of phenolic compounds in vegetable oils were in the range of 83.8-125.9% with inter-day and intra-day precisions of less than 13.2%. It was confirmed that this method was simple, rapid and reliable with an excellent potential for routine analysis of phenolic compounds in oil samples. © 2016 Elsevier Ltd. All rights reserved.


Qin D.-F.,Hubei University | Li T.,Hubei University | Dai C.,Hubei University | Dai C.,Hubei Collaborative Innovation Center for Green Transformation of Bio Resources | Dai C.,Hubei Province Key Laboratory of Regional Development and Environmental Response
Chinese Journal of Applied Ecology | Year: 2015

This study explored whether the degree of pollen limitation was affected by the experimental level (a single flower or inflorescence) and pollen quality (self-pollen or outcross-pollen) of supplemental pollination in Sagittaria trifolia. The results showed that the experimental level caused varying degree of pollen limitation. Compared with the inflorescence level, pollination at the single flower level led to a redistribution of resources among flowers, therefore affecting seed numbers. Pollen quality also played a vital role in the estimation of pollen limitation. Compared with self-pollen, supplemental pollination with outcross-pollen resulted in significantly more seeds and a higher germination rate. This proved that in the research system the reproduction was limited by pollen quality rather than quantity. Our study revealed that both experimental level and pollen quality had effects on the estimation of pollen limitation. It was suggested that in future studies we should evaluate pollen limitation at the inflorescence or whole plant level, and also consider comparing self- and outcross-pollen when applicable. © 2015, Editorial Board of Chinese Journal of Applied Ecology. All right reserved.

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