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Nawaz Z.,Zhejiang University | Kakar K.U.,Zhejiang University | Li X.-B.,Zhejiang Academy of Agricultural Sciences | Li S.,Zhejiang University | And 4 more authors.
Journal of Agricultural and Food Chemistry | Year: 2015

An association mapping of quantitative trait loci (QTLs) regulating the concentrations of eight elements in brown rice (Oryza sativa L.) was performed using USDA mini-core subset cultivated in two different environments. In addition, correlation between the grain elemental concentrations was also studied. A total of 60 marker loci associated with 8 grain elemental concentrations were identified, and these loci were clustered into 37 genomic regions. Twenty new QTLs were found to be associated with important elements such as Zn, Fe, and P, along with others. Fe concentration was associated with the greatest number of markers in two environments. In addition, several important elemental/metal transporter genes were identified in a few mapped regions. Positive correlation was observed within all grain elemental concentrations. In summary, the results provide insight into the genetic basis of rice grain element accumulation and may help in the identification of genes associated with the accumulation of Zn, Fe, and other essential elements in rice. © 2015 American Chemical Society.

Liu L.,Huazhong Agricultural University | Sun G.,Huazhong Agricultural University | Sun G.,Saint Marys University, Halifax | Ren X.,Huazhong Agricultural University | And 3 more authors.
BMC Genetics | Year: 2015

Physiological and morphological traits of flag leaf play important roles in determining crop grain yield and biomass. In order to understand genetic basis controlling physiological and morphological traits of flag leaf, a double haploid (DH) population derived from the cross of Huaai 11×Huadamai 6 was used to detect quantitative trait locus (QTL) underlying 7 physiological and 3 morphological traits at the pre-filling stage in year 2012 and 2013. Results: Total of 38 QTLs distributed on chromosome 1H, 2H, 3H, 4H, 6H and 7H were detected, and explained 6.53% - 31.29% phenotypic variation. The QTLs flanked by marker Bmag829 and GBM1218 on chromosome 2H were associated with net photosynthetic rate (Pn), stomatal conductance (Gs), flag leaf area (LA), flag leaf length (FLL), flag leaf width (FLW), relative chlorophyll content (SPD) and leaf nitrogen concentration (LNC). Conclusion: Two QTL cluster regions associated with physiological and morphological traits, one each on the chromosome 2H and 7H, were observed. The two markers (Bmag829 and GBM1218) may be useful for marker assisted selection (MAS) in barley breeding. © Liu et al.

Xiong Q.,Yangtze University | Xiong Q.,Hubei Collaborative Innovation Center for Grain Industry
Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering | Year: 2015

Sub-surface waterlogging is one of the main agricultural meteorological disasters affecting summer crops in Jianli County, Hubei Province, China. For sub-surface waterlogging is caused by the long-term influence of high soil moisture on crops, it is not easily identified. Few studies were found on the risk evaluation and zonation of crops injured by sub-surface waterlogging. The researchers of this paper firstly adjusted the parameters of DHSVM (distributed hydrology soil vegetation model) by using soil moisture data collected from February 13 to May 20 in 2014 and verified it with the data collected from May 25 to September 23 in 2014. The result showed that the DHSVM could be used to simulate the soil moisture changes in Jianli County. Based on this model, the influencing parameters of waterlogging injury were input, which included meteorology, soil physical properties, groundwater levels, topography, drainage and irrigation conditions, and the farming system. Then the spatial distributions of soil surface moisture were simulated using the DHSVM with a step of 24 h during the growth seasons (March and April) of summer crops from 1970 to 2014. Also the waterlogging moisture indicators were also used to analyze the injury times of each grid in Jianli County (waterlogging injury occurs when the water level is less than 60 cm and surface soil moisture is higher than 95% of soil saturation for 5 days), and the spatial resolution was 90 m. In this way, the spatial distribution of the sub-surface waterlogging injured ratio (SWIR) was calculated, as well as the indicator of the degree of sub-surface waterlogging injury. According to the different range of SWIR value, the research areas which were at the risk of the sub-surface waterlogging injury, were consequently classified into 4 grades: the severe damage zone (SWIR greater than or equal to 0.6), the moderate damage zone (SWIR greater than or equal to 0.3 and less than 0.6), the mild damage zone (SWIR greater than or equal to 0.1 and less than 0.3), and the damage-free zone (SWIR less than 0.1). Thus the zonation of sub-surface waterlogging injury of summer crops was mapped. With some risk evaluation of each zone, the zonation conformed to the actual situation. Since the method used in this paper is based on the model and all the damage factors are taken into account, it is more accurate than the division only using the meteorological elements and proved to be an applicable method for the zonation of sub-surface waterlogging injury on the county scale. ©, 2015, Chinese Society of Agricultural Engineering. All right reserved.

Yu Y.,Capital Normal University | Zhen S.,Capital Normal University | Wang S.,Capital Normal University | Wang Y.,Capital Normal University | And 5 more authors.
BMC Genomics | Year: 2016

Background: Wheat embryo and endosperm play important roles in seed germination, seedling survival, and subsequent vegetative growth. ABA can positively regulate dormancy induction and negatively regulates seed germination at low concentrations, while low H2O2 concentrations promote seed germination of cereal plants. In this report, we performed the first integrative transcriptome analysis of wheat embryo and endosperm responses to ABA and H2O2 stresses. Results: We used the GeneChip® Wheat Genome Array to conduct a comparative transcriptome microarray analysis of the embryo and endosperm of elite Chinese bread wheat cultivar Zhengmai 9023 in response to ABA and H2O2 treatments during seed germination. Transcriptome profiling showed that after H2O2 and ABA treatments, the 64 differentially expressed genes in the embryo were closely related to DNA synthesis, CHO metabolism, hormone metabolism, and protein degradation, while 121 in the endosperm were involved mainly in storage reserves, transport, biotic and abiotic stresses, hormone metabolism, cell wall metabolism, signaling, and development. Scatter plot analysis showed that ABA treatment increased the similarity of regulated patterns between the two tissues, whereas H2O2 treatment decreased the global expression similarity. MapMan analysis provided a global view of changes in several important metabolism pathways (e.g., energy reserves mobilization, cell wall metabolism, and photosynthesis), as well as related functional groups (e.g., cellular processes, hormones, and signaling and transport) in the embryo and endosperm following exposure of seeds to ABA and H2O2 treatments during germination. Quantitative RT-PCR analysis was used to validate the expression patterns of nine differentially expressed genes. Conclusions: Wheat seed germination involves regulation of a large number of genes involved in many functional groups. ABA/H2O2 can repress/promote seed germination by coordinately regulating related gene expression. Our results provide novel insights into the transcriptional regulation mechanisms of embryo and endosperm in response to ABA and H2O2 treatments during seed germination. © 2016 Yu et al.

Hao P.,Capital Normal University | Zhu J.,Capital Normal University | Gu A.,Capital Normal University | Lv D.,Capital Normal University | And 5 more authors.
Proteomics | Year: 2015

Roots, leaves, and intermediate sections between roots and leaves (ISRL) of wheat seedlings show different physiological functions at the protein level. We performed the first integrative proteomic analysis of different tissues of the drought-tolerant wheat cultivar Hanxuan 10 (HX-10) and drought-sensitive cultivar Chinese Spring (CS) during a simulated drought and recovery. Differentially expressed proteins (DEPs) in the roots (122), ISRLs (146), and leaves (163) showed significant changes in expression in response to drought stress and recovery. Numerous DEPs associated with cell defense and detoxifications were significantly regulated in roots and ISRLs, while in leaves, DEPs related to photosynthesis showed significant changes in expression. A significantly larger number of DEPs related to stress defense were upregulated in HX-10 than in CS. Expression of six HSPs potentially related to drought tolerance was significantly upregulated under drought conditions, and these proteins were involved in a complex protein-protein interaction network. Further phosphorylation analysis showed that the phosphorylation levels of HSP60, HSP90, and HOP were upregulated in HX-10 under drought stress. We present an overview of metabolic pathways in wheat seedlings based on abscisic acid signaling and important protein expression patterns. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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