Hubei Collaborative Innovation Center for Freshwater Aquaculture

Wuhan, China

Hubei Collaborative Innovation Center for Freshwater Aquaculture

Wuhan, China
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Li M.,Wuhan Polytechnic University | Li M.,Hubei Collaborative Innovation Center for Freshwater Aquaculture | Sun Z.,Wuhan Polytechnic University | Wang C.,Chinese Academy of Sciences | Zhang L.,Chinese Academy of Fishery Sciences
Journal of Parasitic Diseases | Year: 2016

This paper gave detailed description of the fine structure of Apiosoma piscicola Blanchard 1885. These parasites were collected from the fry of Tachysurus fulvidraco during parasite surveys of fish at Jiangxia Fish Hatchery in Wuhan, Hubei province in May 2011. Its pellicle, peristomial apparatus, nuclei, trochal band and scopula were described, respectively. The relationship between these peritrich ciliates and their hosts was well discussed herein. The scopula of A. piscicola did not adhere directly to the surface of the host epithelial cells, but by means of a secreted adhesive pad of material according to the present study. Besides, no ultrastructural damage to host epithelial cells could be detected in our work. We concluded that these sessilids should be ectocommensals rather than ectoparasites, but not always harmless. In addition, their great variability of body shape and non-strict host specificity were also discussed. © 2016, Indian Society for Parasitology.


Li M.,CAS Wuhan Institute of Hydrobiology | Li M.,Hubei Collaborative Innovation Center for Freshwater Aquaculture | Li C.,Wuhan Polytechnic University | Grim J.N.,Northern Arizona University | And 5 more authors.
European Journal of Protistology | Year: 2017

The morphological revisions of Macrocytopharynxa pyriformis (Nie, 1932) Li et al., 2002; collected from the rectum of Fejervarya limnocharis (=Rana limnocharis), are presented in this paper: (1) two surfaces of the organism are not identical – left side narrower and convex, right broader and flat or slightly concave; (2) infundibulum is large and well-developed with no “fold” or “plicature” present in the middle or posterior portion; (3) micronucleus is tiny and ovoid shaped and always embedded in the middle concavity of macronucleus, which can be well revealed by ammoniacal silver staining. Our phylogenetic analysis based on SSU-rDNA showed that M. pyriformis fell into the Nyctotheroides clade, within which four definite Nyctotheroides species were involved – N. cordiformis, N. deslierresae, N. parvus and N. hubeiensis. In combination with their morphological features, we discussed the reliability of using karyophore organelles or kinetal suture patterns as the generic taxonomic criteria. Besides, we considered that the genus Macrocytopharynxa is a junior synonym of Nyctotheroides and we transfer its type species to Nyctotheroides as Nyctotheroides pyriformis n. comb. The phylogenetic pattern of the family Nyctotheridae was also indicated in our work, but it will be necessary to analyze more species from fishes and reptiles before coming to a sound conclusion. © 2016 Elsevier GmbH


Liu X.,Huazhong Agricultural University | Tang X.,Huazhong Agricultural University | Wang L.,Huazhong Agricultural University | Li J.,Huazhong Agricultural University | And 15 more authors.
Molecular Biology Reports | Year: 2014

Mannose receptor (MR) plays a significant role in innate immune responses to pathogens in vertebrates. Here we characterized the first teleost MR from Megalobrama amblycephala, named maMR and its expression patterns were investigated. The full-length maMR consists of 5,295 bp encoding a putative protein of 1,433 amino acids. The predicted amino acid sequences showed that maMR contained a signal peptide, a cysteine-rich domain, a single fibronectin type II domain, eight tandemly arranged C-type lectin-like domains, a transmembrane domain and a C-terminal cytoplasmic domain. Phylogenetic analysis revealed the highest similarity of maMR with Danio rerio MR predicted by computational analysis. The maMR-mRNAs were ubiquitously transcribed in different tissues, However the highest transcripts were observed in head kidney. Transcripts of maMR significantly increased at the late stages of embryo and continued to be at the high levels after hatching. The maMR transcripts were significantly increased in M. amblycephala after stimulation with killed Aeromonas hydrophila. © 2014 Springer Science+Business Media.


Wang L.,Huazhong Agricultural University | Liu L.,Huazhong Agricultural University | Zhou Y.,Huazhong Agricultural University | Zhao X.,Huazhong Agricultural University | And 14 more authors.
Developmental and Comparative Immunology | Year: 2014

Mannose receptor C type 1 (MRC1) is a pattern-recognition receptor (PRR) which plays a significant role in immune responses. Much work on MRC1 has been done in mammals and birds while little in fish. In this study, we cloned and characterized MRC1 in grass carp (gcMR). The full-length gcMR contained 5291. bp encoding a putative protein of 1432 amino acids. The predicted amino acid sequences showed that gcMR contained a signal peptide, a cysteine-rich (CR) domain, a fibronectin type II (FN II) domain, eight C-type lectin-like domains (CTLDs), a transmembrane domain and a short cytoplasmic domain. gcMR were constitutively expressed in different organs with the higher expression in spleen and head kidney. During embryonic development, gcMR transcript levels were highest at cleavage stage. The up-regulation expression of gcMR, IL-1β and TNF-α in liver, spleen, head kidney and intestine after Aeromonas hydrophila infection indicating it involved in innate immune regulation during bacterial infections. © 2013 .


Gan J.,Chinese Academy of Fishery Sciences | Gan J.,Hubei University | Gan J.,Hubei Collaborative Innovation Center for Freshwater Aquaculture | Lv L.,Chinese Academy of Fishery Sciences | And 5 more authors.
Food Chemistry | Year: 2016

A multiresidue method for the determination of organofluorine pesticides in fish tissue samples was developed and optimized. This method is based on a cleanup step of the extracted samples with acetonitrile, and carried out by solid phase extraction (SPE) with aminopropyl (NH2) cartridge, before the identification and quantification of the residues by liquid chromatography triple quadrupole tandem mass spectrometry (LC-MS/MS). The performance characteristics, such as accuracy, precision, linear range, limits of detection (LOD) and quantification (LOQ), for each pesticide were determined. The proposed method allowed high recoveries (80.4-99.2%) of spiked extracted fat samples at 0.5-10 ng g-1, and very low LODs (between 0.10 and 0.15 ng g-1) and LOQs (between 0.3 and 0.5 ng g-1) determined in the fish samples. The practicality and high sensitivity of this method have been demonstrated in the determination of residues of OFPs in carp fish fed in water containing 10 ng mL-1 OFPs. © 2016 Elsevier Ltd. All rights reserved.


Li M.,Wuhan Polytechnic University | Li M.,Hubei Collaborative Innovation Center for Freshwater Aquaculture | Wang C.,Chinese Academy of Sciences | Grim J.N.,Northern Arizona University | And 2 more authors.
Acta Parasitologica | Year: 2014

A detailed description of the fine structure of Balantidium ctenopharyngodoni Chen, 1955 with an emphasis on its vestibulum is given in the present paper. As to the vestibular kinetids, special attention is paid to the characters of T1, T2 microtubules and nematodesmata. Serving as the major skeleton to the vestibular cortex, the T1, T2 and Pc microtubules are described herein and their support function is also discussed. Moreover, the well-developed nematodesmata of the vestibular kinetids that form a large basket-like complex are described in detail. © 2014 Versita Warsaw and Springer-Verlag Wien.


Wen J.-F.,Huazhong Agricultural University | Wen J.-F.,CAS South China Sea Fisheries Research Institute | Ruan G.-L.,Huazhong Agricultural University | Ruan G.-L.,Yangtze University | And 4 more authors.
Journal of Applied Ichthyology | Year: 2016

In order to test whether Kiss/Kissr systems have potential roles in regulating the embryonic and larval development in teleosts, in this study the Kiss2/Kiss2r full-length cDNA was cloned from blunt snout bream (Megalobrama amblycephala Yih, 1955) by rapid amplification of cDNA ends and their expression patterns were detected in different tissues of adult and developmental stages for the embryonic and larval periods via quantitative real-time PCR. Both Kiss and Kissr genes full-length cDNA sequences of M. amblycephala were obtained and phylogenetic analysis results indicated that these genes belong to the Kiss2/Kiss2r clade. Bioinformatics analyses revealed that there was a conserved decapeptide in M. amblycephala Kiss2 gene putative amino acids, but only two transmembrane domains were predicated in Kiss2r. Tissue distribution analyses showed that both genes were widely expressed in the tissues tested, with high levels in the muscle, gonad and pituitary. At different developmental stages, the mRNA expression of Kiss2/Kiss2r was highest in the blastocyst/15 hpf stage and lowest in the 30 hpf /blastocyst stages for the embryonic period, highest in 7 dph/15 dph and lowest in 30 dph/30 dph for larval period, respectively. These results suggest that the Kiss2/Kiss2r system has varied potential for influencing embryonic and larval development in fish species. © 2016 Blackwell Verlag GmbH.


Li M.,Wuhan Polytechnic University | Li M.,Chinese Academy of Sciences | Li M.,Hubei Collaborative Innovation Center for Freshwater Aquaculture | Li W.,Wuhan Polytechnic University | And 2 more authors.
Korean Journal of Parasitology | Year: 2013

A new trichostome ciliate, Balantidium honghuensis n. sp., was isolated from the recta of Rana nigromaculata and R. limnocharis during parasite surveys in Honghu Lake, Hubei Province, central China in summer of 2010 and 2011. Its detailed morphometric characters based on LM and SEM studies were described herein. The organism is oval in shape and thickly ciliated. The vestibulum is "V" shaped and occupies about 1/3 to 2/5 of the body length. The vestibular and nearby regions possess strong peripheral fibers which form a marked axial fiber about the cytopharynx. More than 10 contractile vacuoles are distributed along the periphery of the latter body. Comparisions were made between this new species and B. sinensis Nie, 1935. They were discriminated from each other in terms of general body forms, body size, and vestibulum shapes. Besides, special attention was paid to its high-speed daughter swarmers which we believed to be the infective stage of B. honghuensis. Possible infection routes of anura amphibian balantidia were discussed. © 2013, Korean Society for Parasitology and Tropical Medicine.


PubMed | Hubei Collaborative Innovation Center for Freshwater Aquaculture and Huazhong Agricultural University
Type: Journal Article | Journal: Developmental and comparative immunology | Year: 2013

Mannose receptor C type 1 (MRC1) is a pattern-recognition receptor (PRR) which plays a significant role in immune responses. Much work on MRC1 has been done in mammals and birds while little in fish. In this study, we cloned and characterized MRC1 in grass carp (gcMR). The full-length gcMR contained 5291bp encoding a putative protein of 1432 amino acids. The predicted amino acid sequences showed that gcMR contained a signal peptide, a cysteine-rich (CR) domain, a fibronectin type II (FN II) domain, eight C-type lectin-like domains (CTLDs), a transmembrane domain and a short cytoplasmic domain. gcMR were constitutively expressed in different organs with the higher expression in spleen and head kidney. During embryonic development, gcMR transcript levels were highest at cleavage stage. The up-regulation expression of gcMR, IL-1 and TNF- in liver, spleen, head kidney and intestine after Aeromonas hydrophila infection indicating it involved in innate immune regulation during bacterial infections.

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