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Gan J.,Chinese Academy of Fishery Sciences | Gan J.,Hubei University | Gan J.,Hubei Collaborative Innovation Center for Freshwater Aquaculture | Lv L.,Chinese Academy of Fishery Sciences | And 5 more authors.
Food Chemistry | Year: 2016

A multiresidue method for the determination of organofluorine pesticides in fish tissue samples was developed and optimized. This method is based on a cleanup step of the extracted samples with acetonitrile, and carried out by solid phase extraction (SPE) with aminopropyl (NH2) cartridge, before the identification and quantification of the residues by liquid chromatography triple quadrupole tandem mass spectrometry (LC-MS/MS). The performance characteristics, such as accuracy, precision, linear range, limits of detection (LOD) and quantification (LOQ), for each pesticide were determined. The proposed method allowed high recoveries (80.4-99.2%) of spiked extracted fat samples at 0.5-10 ng g-1, and very low LODs (between 0.10 and 0.15 ng g-1) and LOQs (between 0.3 and 0.5 ng g-1) determined in the fish samples. The practicality and high sensitivity of this method have been demonstrated in the determination of residues of OFPs in carp fish fed in water containing 10 ng mL-1 OFPs. © 2016 Elsevier Ltd. All rights reserved. Source


Wen J.-F.,Huazhong Agricultural University | Wen J.-F.,CAS South China Sea Fisheries Research Institute | Ruan G.-L.,Huazhong Agricultural University | Ruan G.-L.,Yangtze University | And 4 more authors.
Journal of Applied Ichthyology | Year: 2016

In order to test whether Kiss/Kissr systems have potential roles in regulating the embryonic and larval development in teleosts, in this study the Kiss2/Kiss2r full-length cDNA was cloned from blunt snout bream (Megalobrama amblycephala Yih, 1955) by rapid amplification of cDNA ends and their expression patterns were detected in different tissues of adult and developmental stages for the embryonic and larval periods via quantitative real-time PCR. Both Kiss and Kissr genes full-length cDNA sequences of M. amblycephala were obtained and phylogenetic analysis results indicated that these genes belong to the Kiss2/Kiss2r clade. Bioinformatics analyses revealed that there was a conserved decapeptide in M. amblycephala Kiss2 gene putative amino acids, but only two transmembrane domains were predicated in Kiss2r. Tissue distribution analyses showed that both genes were widely expressed in the tissues tested, with high levels in the muscle, gonad and pituitary. At different developmental stages, the mRNA expression of Kiss2/Kiss2r was highest in the blastocyst/15 hpf stage and lowest in the 30 hpf /blastocyst stages for the embryonic period, highest in 7 dph/15 dph and lowest in 30 dph/30 dph for larval period, respectively. These results suggest that the Kiss2/Kiss2r system has varied potential for influencing embryonic and larval development in fish species. © 2016 Blackwell Verlag GmbH. Source


Li M.,Wuhan Polytechnic University | Li M.,Chinese Academy of Sciences | Li M.,Hubei Collaborative Innovation Center for Freshwater Aquaculture | Li W.,Wuhan Polytechnic University | And 2 more authors.
Korean Journal of Parasitology | Year: 2013

A new trichostome ciliate, Balantidium honghuensis n. sp., was isolated from the recta of Rana nigromaculata and R. limnocharis during parasite surveys in Honghu Lake, Hubei Province, central China in summer of 2010 and 2011. Its detailed morphometric characters based on LM and SEM studies were described herein. The organism is oval in shape and thickly ciliated. The vestibulum is "V" shaped and occupies about 1/3 to 2/5 of the body length. The vestibular and nearby regions possess strong peripheral fibers which form a marked axial fiber about the cytopharynx. More than 10 contractile vacuoles are distributed along the periphery of the latter body. Comparisions were made between this new species and B. sinensis Nie, 1935. They were discriminated from each other in terms of general body forms, body size, and vestibulum shapes. Besides, special attention was paid to its high-speed daughter swarmers which we believed to be the infective stage of B. honghuensis. Possible infection routes of anura amphibian balantidia were discussed. © 2013, Korean Society for Parasitology and Tropical Medicine. Source


Xue C.,Chinese Academy of Fishery Sciences | Xue C.,Nanjing Agricultural University | Xi B.,Chinese Academy of Fishery Sciences | Xi B.,Hubei Collaborative Innovation Center for Freshwater Aquaculture | And 5 more authors.
Chinese Journal of Oceanology and Limnology | Year: 2015

Mucins are important components of mucus, which form a natural, physical, biochemical and semipermeable mucosal layer on the epidermis of fish gills, skin, and the gastrointestinal tract. As the first step towards characterizing the function of Muc2, we cloned a partial Megalobrama amblycephala Muc2 cDNA of 2 175 bp, and analyzed its tissue-specific expression pattern by quantitative real-time PCR (qPCR). The obtained sequence comprised 41 bp 5′-untranslated region (5′-UTR), 2 134 bp open reading frame encoding a protein of 711 amino acids. BLAST searching and phylogenetic analysis showed that the predicted protein contained several common secreted mucin-module domains (VWD-C8-TIL-VWD-C8) and had high homology with mucins from other vertebrates. Among four candidate reference genes (β-Actin, RPI13α, RPII, 18S) for the qPCR, RPII was chosen as an appropriate reference gene because of its lowest variation in different tissues. M. amblycephala Muc2 was mainly expressed in the intestine, in the order (highest to lowest) middle-intestine > fore-intestine > hind-intestine. Muc2 was expressed relatively poorly in other organs (brain, liver, kidney, spleen, skin and gill). Furthermore, after 20-days of starvation, M. amblycephala Muc2 expressions after refeeding for 0 h, 3 h, 16 h, 3 d, and 10 d were significantly decreased in the three intestinal segments (P<0.05) at 16 h, and were then upregulated to near the initial level at 10 d. © 2015, Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg. Source


Li W.,Wuhan Polytechnic University | Wang C.,Chinese Academy of Sciences | Huang F.,Wuhan Polytechnic University | Li M.,Wuhan Polytechnic University | And 5 more authors.
Parasite | Year: 2014

A redescription of Protoopalina pingi Nie, 1935 is presented in this paper to complete Nie's description at both light and scanning electron microscope levels. These organisms were collected from the recta of the frogs Hylarana guentheri Boulenger, 1882 and Pelophylax nigromaculatus Hallowell, 1861 from Jialing River, Sichuan Province and Honghu Lake, Hubei Province, respectively, in China. This is the first record of its occurrence in H. guentheri and P. nigromaculatus. The body of P. pingi is elongated and somewhat spindle-like in shape, slightly narrowed and bluntly rounded at the anterior extremity, while the posterior end is tapering or sharply pointed. The body surface is thickly flagellated, with the caudal tip being barren. The falx, located at the margin of the anterior end, is composed of a narrow band of kinetosomes. Four round or oval-shaped nuclei, usually arranged in a straight line, are situated in the middle region of the body. Comparisons are made between P. pingi and its congeners. © 2014 W. Li et al., published by EDP Sciences. Source

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