Entity

Time filter

Source Type


Li X.-L.,Huazhong University of Science and Technology | Yuan J.,Huazhong University of Science and Technology | Dong Y.-S.,Huazhong University of Science and Technology | Fu C.-H.,Huazhong University of Science and Technology | And 6 more authors.
Journal of Chromatographic Science | Year: 2016

An HPLC method for quantifying total DNA methylation in Taxus chinensis cells is described. Optimal conditions for the method were established as follows: DNA was hydrolyzed with DNA degradase at 37°C for 3 h. The mobile phase was a mixture of Solvent A [50 mM potassium dihydrogen phosphate/triethylamine (100:0.2, v/v)] and Solvent B (methanol); the gradient was 10% (v/v) solvent B. The calibration curves for deoxycytidine monophosphate (dCMP) and methylated dCMP were linear within 1.0-160.0 μg mL-1, with correlation coefficients of 0.9996 and 0.9998. The limits of detection for dCMP and 5-mdCMP were 0.482 and 0.301 ng mL-1, respectively, and the limits of quantification were 1.6 and 1.0 ng mL-1, respectively. The method has been validated according to the current International Conference Harmonization guidelines. The method was able to quantify the content of dCMP and methylated dCMP specifically, accurately and precisely. The global DNA methylation level in different Taxus cells was measured using as little as 3 μg of DNA according to the optimized procedure. In addition, degradation of 5-methylcytosine was prevented. © 2015 The Author 2015. Published by Oxford University Press. Source


Tariq K.,Huazhong Agricultural University | Tariq K.,Hubei Collab Innovation Center For The Characteristic Resources Exploitation Of Dabie Mountains | Noor M.,Huazhong Agricultural University | Saeed S.,Huazhong Agricultural University | And 3 more authors.
Environmental Entomology | Year: 2015

Ultraviolet (UV) light has been used worldwide to monitor and trap insect pests. Whitefly adults show conspicuous positive phototactic behavior toward UV light stimuli; however, knowledge of the effect of UV light exposure on various life-history parameters of Dialeurodes citri remains limited. The present research aimed to investigate the effect of ultraviolet radiation (UV-A; long-wave) exposure on the reproduction and longevity of D. citri adults as well as the development of immature (eggs, larvae, and pupae) flies in the F1 generation. Paired D. citri adults were exposed to UV-A radiation for different periods (0, 1, 4, and 7 h/d) until the end of their life. The results of the experiment revealed that fecundity and oviposition rates increased when adults were irradiated for 1 and 4 h/d, but interestingly, both were significantly decreased compared with those of the controls after the longest exposure time (7 h/d). The longevity of adults of both sexes and the cumulative survival of F1 immatures were decreased with increased exposure time. Exposure to UV-A radiation prolonged the developmental time of immature stages, and a positive correlation was observed with exposure time. Exposure to UV light significantly inhibited egg hatching, larval development, pupation, and adult emergence. To the best of our knowledge, this is the first study describing the effect of UV radiation on a homopteran insect pest. This research may provide a foundation for the scientific community to use UV light in the field as an integrated pest management strategy to control this devastating agricultural pest. © 2015 Crown. Source


Raboanatahiry N.H.,Huazhong University of Science and Technology | Raboanatahiry N.H.,Hubei Collab Innovation Center For The Characteristic Resources Exploitation Of Dabie Mountains | Lu G.,Chinese Academy of Agricultural Sciences | Li M.,Huazhong University of Science and Technology | Li M.,Hubei Collab Innovation Center For The Characteristic Resources Exploitation Of Dabie Mountains
PLoS ONE | Year: 2015

Acyl-coA binding proteins could transport acyl-coA esters from plastid to endoplasmic reticulum, prior to fatty acid biosynthesis, leading to the formation of triacylglycerol. The structure and the subcellular localization of acyl-coA binding proteins (ACBP) in Brassica napus were computationally predicted in this study. Earlier, the structure analysis of ACBPs was limited to the small ACBPs, the current study focused on all four classes of ACBPs. Physicochemical parameters including the size and the length, the intron-exon structure, the isoelectric point, the hydrophobicity, and the amino acid composition were studied. Furthermore, identification of conserved residues and conserved domains were carried out. Secondary structure and tertiary structure of ACBPs were also studied. Finally, subcellular localization of ACBPs was predicted. The findings indicated that the physicochemical parameters and subcellular localizations of ACBPs in Brassica napus were identical to Arabidopsis thaliana. Conserved domain analysis indicated that ACBPs contain two or three kelch domains that belong to different families. Identical residues in acyl-coA binding domains corresponded to eight amino acid residues in all ACBPs of B. napus. However, conserved residues of common ACBPs in all species of animal, plant, bacteria and fungi were only inclusive in small ACBPs. Alpha-helixes were displayed and conserved in all the acyl-coA binding domains, representing almost the half of the protein structure. The findings confirm high similarities in ACBPs between A. thaliana and B. napus, they might share the same functions but loss or gain might be possible. © 2015 Raboanatahiry et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Source


Xiang F.,Huanggang Normal University | Xiang F.,Hubei Collab Innovation Center For The Characteristic Resources Exploitation Of Dabie Mountains | Fang Y.,Huanggang Normal University | Fang Y.,Hubei Collab Innovation Center For The Characteristic Resources Exploitation Of Dabie Mountains | And 2 more authors.
Zeitschrift fur Naturforschung - Section C Journal of Biosciences | Year: 2016

Ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the key enzyme to assimilate CO2 into the biosphere. The nonredundant structural data sets for three RuBisCO domain superfamilies, i.e. large subunit C-terminal domain (LSC), large subunit N-terminal domain (LSN) and small subunit domain (SS), were selected using QR factorization based on the structural alignment with QH as the similarity measure. The structural phylogenies were then constructed to investigate a possible functional significance of the evolutionary diversification. The LSC could have occurred in both bacteria and archaea, and has evolved towards increased complexity in both bacteria and eukaryotes with a 4-helix-2-helix-2-helix bundle being extended into a 5-helix-3-helix-3-helix one at the LSC carboxyl-terminus. The structural variations of LSN could have originated not only in bacteria with a short coil, but also in eukaryotes with a long one. Meanwhile, the SS dendrogram can be contributed to the structural variations at the βA-βB-loop region. All the structural variations observed in the coil regions have influence on catalytic performance or CO2/O2 selectivities of RuBisCOs from different species. Such findings provide insights on RuBisCO improvements. © 2016 by De Gruyter 2016. Source

Discover hidden collaborations