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Tao W.,Wuhan University | Hu S.,Hubei Province Cancer Hospital | Wang Z.,Wuhan University | Fan J.,Wuhan University
Asian Biomedicine | Year: 2010

Background: The genetic factors of colon cancer play an important role in the tumor development and growth. The incidence of colon cancers has greatly increased in China. However, few data is available for the relationship between human muth homolog 1 (hMLH1) gene mutation at site 415 and sporadic colon cancers in Chinese population. Objective: Investigate the relationship between G'!C mutation in hMLH1 gene at site 415 and sporadic colon cancers in Chinese Han population. Methods: Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing techniques, the genotype of the hMLH1 gene was analyzed at site 415 in 97 cases of sporadic colon cancer patients and 138 controls. Reverse-transcription (RT)-PCR was used to determine the level of hMLH1 mRNA expression in normal colonic mucosa of patients with different genotype. Results: The frequency of genotype C/C at the 415 site of the hMLH1 gene was significantly higher in colon cancer patients than in controls. The expression levels of hMLH1 mRNA in normal colonic mucosa were similar in colon cancer patients with different genotypes. Conclusion: G'!C mutation in hMLH1 gene at site 415 may represent a genetic factor that is associated with sporadic colon cancer in a small group of Chinese Han population. Source


Ou W.,Hubei Province Cancer Hospital | Hu S.,Hubei Province Cancer Hospital | Yu W.,Hubei Province Cancer Hospital | Wu Y.,Hubei Province Cancer Hospital
Medical Journal of Wuhan University | Year: 2011

Objective: To study the expression of Twist and Trkb in human breast cancers, and to investigate the role of Twist and Trkb in breast cancer progression, metastasis, and prognosis. Methods: The MCF-7 human breast cancer cell line was cultured under mammosphere forming conditions and implanted into nude mice. RT-PCR technique was used to measure the expression of Twist and Trkb mRNA in the tissue samples from 14 nude mice and 86 human human breast tumors. Results: In the nude mice implanted with breast cancer cells, the level of the Twist and Trkb mRNA in the samples from metastasis tissues was higher than that of primary tissues (the relative OD value of Twist and Trkb mRNA was 2.1 ± 0.2 vs 1.4 ± 0.2, 2.3 ± 0.4 vs 1.5 ± 0.3; t = 2.4, 2.8; P = 0.03, 0.02 respectively). In human tumor samples, the level of the Twist and Trkb mRNA of lymph node metastasis was higher than that of breast tissues (1.67 ± 0.32 vs 0.83 ± 0.23, 1.58 ± 0.41 vs 0.65 ± 0.24 respectively; t = 2.6, P = 0.02; t = 3.01, P = 0.01). The level of the Twist and Trkb mRNA of breast cancers was higher than that of primary tissues (2.38 ± 0.22 vs 1. 84 ± 0.43, 2.17 ± 0.41 vs 1.62 ± 0.32 respectively; t = 3.23, P = 0.01; t = 2.84, P = 0.03). The expression of Twist and Trkb was significantly correlated with clinical stages, tumor lymph node metastasis and loss of differentiation (all P < 0.05). However, there was no correlation between Twist or Trkb mRNA levels and age or tumor size (all P > 0.05). Increased expression of Twist correlated with shorter overall survival ([28.4 ± 2.2] vs [32.7 ± 2.4] months, LogRank=3.88, P = 0.037), while no significant correlations were observed between increased expression of Trkb and shorter overall survival([29.9 ± 2.6] vs [31.5 ± 2.5] months, LogRank=2.46, P = 0.08). Conclusion: Expression of Twist and Trkb associate with breast cancer infiltration and metastasis, and increased expression of Twist may implicate in breast cancer prognosis. Source

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