Tang M.X.,Chinese Academy of Agricultural Sciences |
Zheng X.M.,Hubei Academy of Agriculture science |
Hou J.,China Agricultural University |
Qian L.L.,Chinese Academy of Agricultural Sciences |
And 3 more authors.
Theriogenology | Year: 2013
We previously generated and characterized synthesized fatty acid desaturase-1 (sFat-1) transgenic pigs that had increased concentrations of ω-3 unsaturated fatty acid in their meat. The objective was to assess whether the inserted foreign gene in sFat-1 transgenic pigs was able to transfer and integrate into the genome of nontransgenic pigs by suckling or mating. Tests for suckling-mediated horizontal gene transfer (HGT) included sFat-1 transgenic sows nursing nontransgenic piglets and sFat-1 transgenic piglets suckling nontransgenic sows. Tests for mating-mediated HGT were performed by male sFat-1 transgenic pigs mated with nontransgenic females and female sFat-1 transgenic pigs mated with nontransgenic males. Polymerase chain reaction was used to detect the sFat-1 gene fragment in various tissues sampled from nontransgenic pigs. The foreign target gene sFat-1 was not detected in the genomic DNA of various tissues and organs sampled from nontransgenic pigs. Therefore, we concluded that HGT from transgenic pigs to wild type pigs via suckling or mating was unlikely. © 2013 Elsevier Inc.
PubMed | Yangtze University, Huazhong Agricultural University, Hubei Academy of Agriculture science and China Agricultural University
Type: | Journal: BMC plant biology | Year: 2016
Vitreoscilla hemoglobin (VHb) is a type of hemoglobin found in the Gram-negative aerobic bacterium Vitreoscilla that has been shown to contribute to the tolerance of anaerobic stress in multiple plant species. Maize (Zea mays L.) is susceptible to waterlogging, causing significant yield loss. In this study, we approached this problem with the introduction of an exogenous VHb gene.We overexpressed the VHb gene in Arabidopsis and maize under the control of the CaMV35S promoter. After 14days of waterlogging treatment, the transgenic VHb Arabidopsis plants remained green, while the controls died. Under waterlogging, important plant growth traits of VHb plants, including seedling height, primary root length, lateral root number, and shoot dry weight were significantly improved relative to those of the controls. The VHb gene was also introduced into a maize line through particle bombardment and was then transferred to two elite maize inbred lines through marker-assisted backcrossing. The introduction of VHb significantly enhanced plant growth under waterlogging stress on traits, including seedling height, primary root length, lateral root number, root dry weight, and shoot dry weight, in both Zheng58 and CML50 maize backgrounds. Under the waterlogging condition, transgenic VHb maize seedlings exhibited elevated expression of alcohol dehydrogenase (ADH1) and higher peroxidase (POD) enzyme activity. The two VHb-containing lines, Zheng58 (VHb) and CML50 (VHb), exhibited higher tolerance to waterlogging than their negative control lines (Zheng58 and CML50).These results demonstrate that the exogenous VHb gene confers waterlogging tolerance to the transgenic maize line. In Maize in the place of to the transgenic maize line, the VHb gene is a useful molecular tool for the improvement of waterlogging and submergence-tolerance.
Chen Y.,Hubei Academy of Agriculture Science |
Hu C.,Hubei Academy of Agriculture Science |
Li S.,Hubei Academy of Agriculture Science |
Qiao Y.,Hubei Academy of Agriculture Science
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2011
The soil food web consists of assemblage of interactions within soil organisms. Most ecological researches focused on the theory with respect to connectedness web, energy flow web and functional web. However, practical applications for established theories to guide the agriculture practice were seldom used. In nature ecosystem, the soil food web play an important role of maintaining both productivity and ecosystem health. In farmland, however, most of these functions were replaced by agrochemicals such as fertilizer and pesticides, which result in many environment problems. In order to maintain high crop yield and protect environmental disturbances, effective soil food web management is necessary. This paper reviewed mechanisms of controlling the soil food web, direct and indirect methods of measuring the soil food web, and how to regulate the soil food web to healthy status. Understanding these mechanisms provide the theory support and practice guide of achieving a healthy soil food web. To address the mechanism of controlling the soil food web, the paper argued the top-down and bottom-up regulation of soil food web structure and emphasized that bottom-up effects maybe more important in farmland. Also the bacterial and fungi channel within the soil food web were compared. Regarding the methods of measuring the soil food web, mainly focused on the indirect method that uses nematodes as bio-indicators of soil food web structure, enrichment and decomposition channels. Finally, we discussed mechanisms describing how to get the healthy soil food web by regulating the quantity and quality of debris or functional groups by compos tea and a concept mode of managing soil food web are discussed.
Ren H.-Y.,Chinese Academy of Agricultural Sciences |
Zheng X.-M.,Hubei Academy of Agriculture science |
Chen H.-X.,Beijing Institute of Biotechnology |
Li K.,Chinese Academy of Agricultural Sciences
Agricultural Sciences in China | Year: 2011
Polyunsaturated fatty acids (PUFAs) are essential for normal growth in mammals, especially the co-3 PUFAs, which play important roles in preventing several life-threatening diseases, such as coronary heart disease and diabetes. In this study, we aimed to investigate whether the sFat-1 gene from Caenorhabditis briggsae could be functionally expressed in transgenic pigs, and whether the transgenic could synthesize high quality co-3 PUFAs endogenously. In this study, a gene construct consisting of CMV promoter and 1.9 kb cDNA of co-3 fatty acid desaturase gene (sFat-1) from C. briggsae was injected into the male pronucleus of pig embryos by micro injection. The piglets were screened for the transgene by PCR, Southern blot and reverse transcription-PCR analysis. Pigs that give positive results were mated with wild-type pigs to produce the next generation and the transmission of transgene was examined by PCR analysis. Fatty acids compositions of various tissues in the transgenic pigs were then analyzed by gas Chromatograph. A total of 878 embryos were transferred into 42 recipients, among which 29 successfully got pregnant and gave birth to a total of 162 piglets, and 8 of them were identified to be transgenic. Fatty acid compositions in the transgenic pigs were altered, and the levels of co-6: co-3 ratios were decreased from 14.53 in the control to 2.62 in Fat-1 transgenic pigs. A number of primary sFa/-./-transgenic pigs were bred in this study, which lays the foundation for cultivation of new varieties of transgenic pigs. © 2011 Chinese Academy of Agricultural Sciences.
Li Y.,Huazhong Agricultural University |
Mei S.,Hubei Academy of Agriculture Science |
Zhang X.,Huazhong Agricultural University |
Peng X.,Hubei Academy of Agriculture Science |
And 6 more authors.
BMC Genomics | Year: 2012
Background: Recent studies have shown that copy number variation (CNV) in mammalian genomes contributes to phenotypic diversity, including health and disease status. In domestic pigs, CNV has been catalogued by several reports, but the extent of CNV and the phenotypic effects are far from clear. The goal of this study was to identify CNV regions (CNVRs) in pigs based on array comparative genome hybridization (aCGH).Results: Here a custom-made tiling oligo-nucleotide array was used with a median probe spacing of 2506 bp for screening 12 pigs including 3 Chinese native pigs (one Chinese Erhualian, one Tongcheng and one Yangxin pig), 5 European pigs (one Large White, one Pietrain, one White Duroc and two Landrace pigs), 2 synthetic pigs (Chinese new line DIV pigs) and 2 crossbred pigs (Landrace × DIV pigs) with a Duroc pig as the reference. Two hundred and fifty-nine CNVRs across chromosomes 1-18 and X were identified, with an average size of 65.07 kb and a median size of 98.74 kb, covering 16.85 Mb or 0.74% of the whole genome. Concerning copy number status, 93 (35.91%) CNVRs were called as gains, 140 (54.05%) were called as losses and the remaining 26 (10.04%) were called as both gains and losses. Of all detected CNVRs, 171 (66.02%) and 34 (13.13%) CNVRs directly overlapped with Sus scrofa duplicated sequences and pig QTLs, respectively. The CNVRs encompassed 372 full length Ensembl transcripts. Two CNVRs identified by aCGH were validated using real-time quantitative PCR (qPCR).Conclusions: Using 720 K array CGH (aCGH) we described a map of porcine CNVs which facilitated the identification of structural variations for important phenotypes and the assessment of the genetic diversity of pigs. © 2012 Li et al.; licensee BioMed Central Ltd.
Yi J.,Huazhong Agricultural University |
Wu H.-Y.,Hubei Academy of Agriculture science |
Wu J.,Huazhong Agricultural University |
Deng C.-Y.,Huazhong Agricultural University |
And 2 more authors.
Applied Microbiology and Biotechnology | Year: 2012
In order to obtain the diversity and temporal-spatial distribution of Bacillus community during the swine manure composting, we utilized traditional culture methods and the modern molecular biology techniques of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and -denaturing gradient gel electrophoresis (PCR-DGGE). Bacillus species were firstly isolated from the composting. Based on temperature changes, the temporal-spatial characteristics of total culturable Bacillus were remarkable that the number of the culturable Bacillus detected at the high-temperature stage was the highest in each layer of the pile and that detected in the middle layer was the lowest at each stage of composting respectively. The diversity of cultivated Bacillus species isolated from different composting stages was low. A total of 540 isolates were classified by the RFLP method and partial 16S rDNA sequences. They affiliated to eight species including Bacillus subtilis, Bacillus cereus, Bacillus thuringiensis, Bacillus anthracis, Bacillus megaterium, Bacillus licheniformis, Bacillus pumilus, and Bacillus circulans. The predominant species was B. subtilis, and the diversity of culturable Bacillus isolated in the middle-level samples at temperature rising and cooling stages was the highest. The DGGE profile and clone library analysis revealed that the temporal-spatial distribution of Bacillus community was not obvious, species belonging to the Bacillus were dominant (67%) with unculturable bacteria and B. cereus was the second major culturable Bacillus species. This study indicated that a combination of culture and culture-independent approaches could be very useful for monitoring the diversity and temporal-spatial distribution of Bacillus community during the composting process. © 2011 Springer-Verlag.
Qiao M.,Huazhong Agricultural University |
Wu H.-Y.,Hubei Academy of Agriculture Science |
Li F.-E.,Huazhong Agricultural University |
Jiang S.-W.,Huazhong Agricultural University |
And 2 more authors.
Molecular Biology Reports | Year: 2010
The lecithin cholesterol acyltransferase gene (LCAT) plays an important role in lipoprotein metabolism, especially in the process termed 'reverse cholesterol transport'. In this study, we obtained the 1,434 bp mRNA sequence of porcine LCAT including the full coding region and encoding a protein of 472 amino acids. The sequence was deposited into the GenBank under the accession no. EU717835. The genomic sequence of this gene which contains six exons and five introns, is 3,712 bp in length (GQ379050). Bioinformatic analysis of the 5′ regulatory region has revealed that some transcription factor Sp1, AP-1, AP-2 and NF-kappaB were represented in this region. Tissue expression analysis showed that the porcine LCAT gene is ubiquitously expressed in all examined tissues. Phylogenetic tree was constructed by aligning the amino acid sequences of different species. Moreover, we found a single nucleotide polymorphism (SNP, C/G266) in intron 1 of the LCAT gene and association analysis showed that it was significantly associated with ratio of lean to fat (P < 0.05), caul fat weight (P < 0.01), leaf fat weight (P < 0.05), carcass length (P < 0.05) and bone percentage (P < 0.05). Our study will lay the groundwork for the further investigations on the detailed physiological function of LCAT in pig models. © 2009 Springer Science+Business Media B.V.
Zhang R.,Hubei Academy of Agriculture science |
Li X.,Hubei Academy of Agriculture science
Molecular Biology Reports | Year: 2011
The objective of this study was to estimate the allele and genotype frequencies of the IGF-IR/TaqI, m-calpain/HhaI, and UCP-3/BglI polymorphisms and to determine associations between these polymorphisms and growth traits in Chinese indigenous cattle breeds. Genotyping was performed on 321 animals including 135 Nanyang, 80 Qinchuan, and 106 Jiaxian cattle. No significant differences in growth traits were observed between the genotypes of IGF-IR/TaqI polymorphism in Nanyang cattle. The m-calpain/HhaI, and UCP-3/BglI polymorphisms were associated with body weight, withers height, and body length of 6 months (P < 0.05 or P < 0.01), body length of 18 months (P < 0.05), and body length and heart girth of 24 months (P < 0.01) in Nanyang cattle. In addition, Nanyang cattle aged 12 months with AB genotype of m-calpain/HhaI polymorphism had higher body length and heart girth than those with BB genotype (P < 0.01). The withers height were greater (P < 0.01) in Nanyang cattle aged 12 months with genotype AB of UCP-3/BglI polymorphism than those with genotype BB. The Nanyang cattle aged 24 months with genotype AA had higher withers height than those with genotype BB (P < 0.05). In conclusion, the m-calpain/HhaI and UCP-3/BglI polymorphisms may be used as DNA markers for selection in the breeding process of Nanyang cattle. © 2010 Springer Science+Business Media B.V.
Sun X.,Huazhong Agricultural University |
Mei S.,Hubei Academy of Agriculture Science |
Tao H.,Huazhong Agricultural University |
Wang G.,Huazhong Agricultural University |
And 5 more authors.
BMC Genomics | Year: 2011
Background: The Chinese Taihu is one of the most prolific pig breeds in the world, which farrows at least five more piglets per litter than Western pig breeds partly due to a greater ovulation rate. Variation of ovulation rate maybe associated with the differences in the transcriptome of Chinese Taihu and Large White ovaries. In order to understand the molecular basis of the greater ovulation rate of Chinese Taihu sows, expression profiling experiments were conducted to identify differentially expressed genes in ovarian follicles at the preovulatory stage of a PMSG-hCG stimulated estrous cycle from 3 Chinese Taihu and 3 Large White cycling sows by using the Affymetrix Porcine Genechip™.Results: One hundred and thirty-three differentially expressed genes were identified between Chinese Taihu and Large White sows by using Affymetrix porcine GeneChip (p ≤ 0.05, Fold change ≥ 2 or ≤ 0.5). Gene Ontology (GO) analysis revealed that these genes belonged to the class of genes that participated in regulation of cellular process, regulation of biological process, biological regulation, developmental process, cell communication and signal transduction and so on. Significant differential expression of 6 genes including WNT10B and DKK2 in the WNT signaling pathway was detected. Real-time RT-PCR confirmed the expression pattern in seven of eight selected genes. A search of chromosomal location revealed that 92 differentially expressed transcripts located to the intervals of quantitative trait loci (QTLs) for reproduction traits. Furthermore, SNPs of two differentially expressed genes- BAX and BMPR1B were showed to be associated with litter size traits in Large White pigs and Chinese DIV line pigs (p ≤ 0.1 or p ≤ 0.05).Conclusions: Our study detected many genes that showed differential expression between ovary follicles of two divergent breeds of pigs. Genes involved with regulation of cellular process, regulation of biological process, in addition to several genes not previously associated with ovarian physiology or with unknown function, were differentially expressed between two breeds. The suggestive or significant associations of BAX and BMPR1B gene with litter size indicated these genetic markers had the potentials to be used in pig industry after further validation of their genetic effects. Taken together, this study reveals many potential avenues of investigation for seeking new insights into ovarian physiology and the genetic control of reproduction. © 2011 Sun et al; licensee BioMed Central Ltd.
PubMed | Huazhong Agricultural University and Hubei Academy of Agriculture science
Type: Journal Article | Journal: Journal of Zhejiang University. Science. B | Year: 2016
The laying quail is a worldwide breed which exhibits high economic value. In our current study, the vasoactive intestinal peptide receptor-1 (VIPR-1) was selected as the candidate gene for identifying traits of egg production. A single nucleotide polymorphism (SNP) detection was performed in 443 individual quails, including 196 quails from the H line, 202 quails from the L line, and 45 wild quails. The SNPs were genotyped using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Two mutations (G373T, A313G) were detected in all the tested quail populations. The associated analysis showed that the SNP genotypes of the VIPR-1 gene were significantly linked with the egg weight of G373T and A313G in 398 quails. The quails with the genotype GG always exhibited the largest egg weight for the two mutations in the H and L lines. Linkage disequilibrium (LD) analysis indicated that G373T and A313G loci showed the weakest LD. Seven main diplotypes from the four main reconstructed haplotypes were observed, indicating a significant association of diplotypes with egg weight. Quails with the h1h2 (GGGT) diplotype always exhibited the smallest egg weight and largest egg number at 20 weeks of age. The overall results suggest that the alterations in quails may be linked with potential major loci or genes affecting reproductive traits.