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Hubei Academy Of Agricultural Science | Date: 2017-04-12

Disclosed is a method for modification of heat resistance of Newcastle disease virus and use thereof, according to which heat resistance of Newcastle disease virus can be distinctly improved through replacement of HN genes. The present disclosure takes advantage of reverse genetic manipulation technique. Modification of non-heat-resistant Newcastle disease virus is based on modification of transcription plasmid, which is achieved through replacing a HN gene of a non-heat-resistant Newcastle disease virus with a HN gene of a heat-resistant Newcastle disease virus. The modified transcription plasmid is used to transfect a host cell. A recombinant Newcastle disease virus is rescued and obtained. The recombinant Newcastle disease virus has a distinctly improved heat resistance. The present disclosure, for the first time, enormously improves the heat resistance characteristic of Newcastle disease virus through artificial recombinant, and successfully transforms the non-heat-resistant Newcastle disease vaccine LaSota strain into a heat-resistant Newcastle disease vaccines rT-HN strain. The method provided by the present disclosure can be widely used in modification of heat resistance of Newcastle disease virus strains, and has a broad application prospect in developing heat-resistant, safe, and efficient Newcastle disease vaccines.

Jiang M.,Auburn University | Liu S.,Auburn University | Du X.,Hubei Academy of Agricultural Science | Wang Y.,Auburn University
Food Hydrocolloids | Year: 2010

Gelatin from catfish skin was obtained by thermal extraction. Triacetin was added to the gelatin at 0, 50, 100, and 150% of the gelatin content to improve the hydrophobic properties of the resulting films. Tween 80 (10% of triacetin amount) was also added as an emulsifier. Internal microstructures of the films were examined using a transmission X-ray microscope (TXM). Other film properties, such as thickness, mechanical properties, water vapor permeability, water solubility, light transparency, and thermal properties were also evaluated. Possible relationships between the internal microstructures and the film properties were hypothesized. The triacetin distribution changed from homogeneous to heterogeneous with its increased content in the films. The addition of triacetin resulted in decreased tensile strength (TS) and increased percent elongation (%E), water solubility, UV and visible light barrier properties, and protein denaturation temperature of the films. Water vapor permeability of the films increased in some treatments (100% and 150% triacetin) possibly due to the heterogeneous distribution of the triacetin and also the increased Tween 80 amount in the films. © 2009 Elsevier Ltd. All rights reserved.

Hu C.,Hubei Academy of Agricultural science | Qi Y.,Huazhong Agricultural University
European Journal of Agronomy | Year: 2013

An eleven years long-term field experiment for soil fertility and crop yield improvement had been conducted at China Agricultural University's Qu-Zhou experiment station since 1993. The field experiment included three treatments: effective microorganisms (EM) compost treatment; traditional compost treatment; and unfertilized control. The results revealed that long-term application of EM compost gave the highest values for the measured parameters and the lowest values in the control plot. The application of EM in combination with compost significantly increased wheat straw biomass, grain yields, straw and grain nutrition compared with traditional compost and control treatment. Wheat straw biomass, grain yields, straw and grain nutrition were significantly higher in compost soils than in untreated soil. This study indicated that application of EM significantly increased the efficiency of organic nutrient sources. © 2012 Elsevier B.V.

Zhao Q.,Wuhan University | Zou J.,Huazhong Agricultural University | Meng J.,Huazhong Agricultural University | Mei S.,Hubei Academy of Agricultural Science | Wang J.,Wuhan University
PLoS ONE | Year: 2013

Polyploidization has played an important role in plant evolution and speciation, and newly formed allopolyploids have experienced rapid transcriptomic changes. Here, we compared the transcriptomic differences between a synthetic Brassica allohexaploid and its parents using a high-throughput RNA-Seq method. A total of 35,644,409 sequence reads were generated, and 32,642 genes were aligned from the data. Totals of 29,260, 29,060, and 29,697 genes were identified in Brassica rapa, Brassica carinata, and Brassica allohexaploid, respectively. We compared 7,397 differentially expressed genes (DEGs) between Brassica hexaploid and its parents, as well as 2,545 nonadditive genes of Brassica hexaploid. We hypothesized that the higher ploidy level as well as secondary polyploidy might have influenced these changes. The majority of the 3,184 DEGs between Brassica hexaploid and its paternal parent, B.rapa, were involved in the biosynthesis of secondary metabolites, plant-pathogen interactions, photosynthesis, and circadian rhythm. Among the 2,233 DEGs between Brassica hexaploid and its maternal parent, B. carinata, several played roles in plant-pathogen interactions, plant hormone signal transduction, ribosomes, limonene and pinene degradation, photosynthesis, and biosynthesis of secondary metabolites. There were more significant differences in gene expression between the allohexaploid and its paternal parent than between it and its maternal parent, possibly partly because of cytoplasmic and maternal effects. Specific functional categories were enriched among the 2,545 nonadditive genes of Brassica hexaploid compared with the additive genes; the categories included response to stimulus, immune system process, cellular process, metabolic process, rhythmic process, and pigmentation. Many transcription factor genes, methyltransferases, and methylation genes showed differential expression between Brassica hexaploid and its parents. Our results demonstrate that the Brassica allohexaploid can generate extensive transcriptomic diversity compared with its parents. These changes may contribute to the normal growth and reproduction of allohexaploids. © 2013 Zhao et al.

Ke S.,Hubei Academy of Agricultural science
Journal of Molecular Structure | Year: 2016

The novel salicylamide-type ligand containing bis-pyridine moieties, i.e. 2-((6-chloropyridin-3-yl)methoxy)-N-(2-((6-chloropyridin-3-yl)methylthio)phenyl)benzamide, which has been successfully synthesized and characterized by typical spectroscopic techniques mainly including IR, 1H NMR and ESI-MS. The structure of target compound was further determined by single crystal X-ray diffraction method and which crystallized in the monoclinic system with space group P2(1)/c. © 2016 Elsevier B.V. All rights reserved.

Hu C.,Hubei Academy of Agricultural science | Qi Y.,Huazhong Agricultural University
Agronomy for Sustainable Development | Year: 2013

Excessive application of mineral fertilizers wastes resources and contaminates the environment. Alternative natural substitutes could solve those issues. Here, we hypothesize that organic compost may increase soil biological fertility more effectively than mineral fertilizers, and that effective microorganisms could improve the effects of traditional compost. So far, few investigations have analyzed the effects of the effective microorganisms on soil fauna such as nematodes. A 1997-2004 field experiment of soil fertility and crop yield has been carried out at China Agricultural University's Qu-Zhou experimental station. A randomized block experiment comprised effective microorganisms, compost, traditional compost, N and P fertilizer treatment, and untreated controls. Soil nematode community structure and wheat yields were analyzed during wheat growth stages. Results show that in May the total nematode number is 43.21 % higher for effective microorganisms compost plots compared with traditional compost plots. Soil free-living nematodes are 29.32 % more abundant and bacteria-feeding nematodes are 63.23 % more abundant for effective microorganisms compost plots compared with traditional compost plots in June. Wheat grain yield is correlated with soil free-living nematodes during the jointing stage of wheat growth, with a correlation coefficient R 2 of 0.88. © 2013 INRA and Springer-Verlag France.

Hu C.,Hubei Academy of Agricultural science | Qi Y.,Huazhong Agricultural University
European Journal of Soil Biology | Year: 2010

Nematode density and biodiversity in maize field soil treated with compost, chemical fertilizer and with no amendments were investigated in a multi-year field experiment at the Qu-Zhou experimental station, China Agricultural University. The soils were collected from the upper (0-20 cm) soil layer during the maize growing stages in 2004. The results demonstrated that significant differences for the total nematode density, bacterivores, fungivores, plant parasites and omnivores-predators density were found between treatments and between dates. The total nematode density and bacterivores density were greater in compost-treated soil than in chemical fertilizer-treated soil, and were greater in chemical fertilizer-treated soil than in control soil during all sampling periods. The total nematodes density ranged from 106 to 657 individuals per 100 g dry soil in the present study. Total 40 nematode genera were found in all treatments and sampling periods, and 12 genera were bacterivores, 4 genera were fungivores, 16 genera were plant parasites and 8 genera were omnivores-predators. Cephalobus, Rhabditis, Tylenchorhynchus, Pratylenchus, Helicotylenchus and Rotylenchus were dominant genera in present study. The plant parasites and bacterivores were dominant trophic groups. The ratio of bacterivores plus fungivores to plant parasites was higher in compost-treated soil compared to chemical fertilizer-treated soil except October. Maturity index and combined maturity index were lower in compost-treated soil compared to chemical fertilizer-treated and control soil except July. The plant parasite index was higher in compost-treated soil compared to chemical fertilizer-treated soil except July. The multi-year application of compost and chemical fertilizer had effected on soil nematode population density and community structure. © 2010 Elsevier Masson SAS.

Hu C.,Hubei Academy of Agricultural science | Qi Y.C.,Huazhong Agricultural University
Helminthologia | Year: 2010

Summary: Nematode abundance and diversity from different types of organic manure soil treatments were investigated in a longterm field experiment carried out in Qu-Zhou experimental station, China Agricultural University. The composts used in the experiment were a traditional compost (C) (60 % straw, 30 % livestock dung, 5 % cottonseed-pressed trash and 5 % brans), traditional compost and chicken dung compost (60 % straw, 30 % chiken dung, 5 % cottonseedpressed trash and 5 % brans) added with effective microorganisms, EMC and EMCDC respectively. Six treatments were arranged according to a randomized block design with three replicates per treatment. Treatments were incorporation into the soil of compost EMC, EMCDC, and C each at the rates of 7.5 and 15 t/ha. Plots were sown with winter wheat (Triticum aestivum L.) and summer maize (Zea mays L.) every year from 1997 to 2004. Overall, 28 nematode genera were found. Seven genera were bacterivores, 3 genera were fungivores, 13 genera were plantparasites and 5 genera were omnivores-predators. The Rhabditis, Cephalobus, Helicotylenchus were dominant genera in the present study. The bacterivores and plantparasitic nematodes were the dominant trophic groups except in C treatment. Nematode abundance per 100 g dried soil ranged from 372 to 553. Addition of effective microorganism increased the number and proportion of bacterivorous nematodes and decreased the number and proportion of plant-parasitic nematodes compared to traditional compost C. Total number of nematode was significantly influenced by compost amount, but didn't significantly influence by EM agent. Total abundance of nematode was positively correlated with the content of soil organic matter, total N, available P and K. The long-term addition of EM agent hasn't adverse effect on soil nematode community. © 2010 Parasitological Institute of SAS.

Zhao Z.,Nanjing University | Wang Q.,Nanjing University | Wang K.,Hubei Academy of Agricultural science | Brian K.,Hill International | And 2 more authors.
Bioresource Technology | Year: 2010

An endophytic Bacillus vallismortis ZZ185 was isolated from healthy stems of the plant Broadleaf Holly (Ilex latifolia Thunb) collected in Nanjing, China. Both the culture filtrate and the n-butanol extract of strain ZZ185 showed strong growth inhibition activity in vitro against the phytopathogens Fusarium graminearum, Alternaria alternata, Rhizoctonia solani, Cryphonectria parasitica and Phytophthora capsici. The results showed that the filtrate and extract reduced the symptoms of wheat seedlings infected with A. alternata and F. graminearum by about 90% and 50%, respectively, based on the comparison of the lengths of zones on the seminal roots showing cortical browning with those of the roots of uninfected controls. The antifungal activity of the culture filtrate was significantly correlated with cell growth of strain ZZ185. The active metabolite in the filtrate was relatively thermally stable with more than 50% of the antifungal activity of the culture filtrate being retained even after being held at 121 °C for 30 min. Meanwhile, the antifungal activity of the filtrate against the growth of A. alternata and F. graminearum remained almost unchanged (>75%) when the culture was exposed to a pH ranging from 1 to 8, but significantly reduced after the filtrate had been exposed to basic conditions. From the n-butanol extract of the filtrate, the antifungal compounds were isolated as a mixture of Bacillomycin D (n-C14) and Bacillomycin D (iso-C15). The strong antifungal activity implied that the endophytic B. vallismortis ZZ185 and its bioactive components might provide an alternative resource for the biocontrol of plant diseases. © 2009 Elsevier Ltd.

Hubei Academy Of Agricultural Science | Date: 2013-06-24

A heat-resistant NDV live vaccine vector system includes a transcription plasmid, three helper plasmids, and host cells. The transcription plasmid is constructed by through cloning complete genomic cDNA of a heat-resistant NDV vaccine strain to a pBR322 vector. The three helper plasmids are constructed by cloning sequences coding nucleoprotein (NP), phosphoprotein, large polymerase protein of a heat-resistant NDV vaccine strain respectively to pcDNA3.1 vectors. A recombinant NDV artificially obtained by cotransfacting host cells with the transcription plasmid and the three helper plasmids shows heat-resistance.

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