Huazhong Agricultural University is a multi-disciplinary comprehensive university giving priority to agriculture, characterized by life science and supplemented by the combination of agriculture, basic science, engineering, liberal arts, law, economic trade, and management. HAU, one of the first groups of universities in China which are authorized to confer Ph.D. and M.A. degrees, has produced the new China's first doctor majoring in agronomy. Firmly adhering to the two central tasks like teaching and scientific research, HAU maintains its management by levels and flexible forms. As far as education quality and academic level, HAU ranks first among the agricultural universities in China. In addition, it has been converted into a nationally important base for training senior special agricultural personnel and developing agricultural science and technology. Wikipedia.
Huazhong Agricultural University | Date: 2014-08-25
The present invention relates to an isolated major gene GS3 which regulates grain weight and grain length in the rice and the cloning of said gene. The DNA sequence of GS3 gene is as shown in SEQ ID NO. 1 and is 7883 bp in length. GS3 gene comprises 5 exons and encodes 232 amino acids. It is predicted based on bioinformatics analysis that said protein contains conserved domains including a PEBP-like domain, a transmembrane domain, a cysteine-rich domain of TNFR/NGFR and a VWFC domain. cDNA sequence of said gene is as shown in SEQ ID NO. 2. By sequence alignment between three large grain species and 3 small grain species of rice, it is revealed there is only one common single nucleotide mutation in a 7.9-kb region between the two different grain-length groups. Said nucleotide mutation is located at the second exon of the GS3 gene, in which a cysteine codon (TGC) in the small-grain group is mutated to a termination codon (TGA) in the large-grain group. This mutation causes a premature termination in the large-grain group which leads to a 178-amino acids truncation (including part of the PEBP-like domain and all the other three conserved domains). The present invention also provides methods of producing transgenic plants comprising sequences disclosed herein.
Huazhong Agricultural University | Date: 2012-04-16
Rice OXHS4 gene controlling root growth and conferring enhanced drought resistance, and use thereof in genetic improvement of rice drought resistance is provided. OXHS4 gene is cloned using candidate gene screening method. Drought stress experiments at seedling stage and adult plant stage shows that overexpression of OXHS4 gene can improve drought resistance of transgenic rice, while the oxhs4 mutant exhibits sensitivity to drought, showing the function of said gene and the use thereof.
Huazhong Agricultural University | Date: 2010-04-14
The present invention pertains to the field of rice genetic engineering. Specifically, the present invention relates to a rice OsNHAD gene that enhances tolerance to salt stress, which was obtained through gene isolation, cloning and function verification, and also to use of the gene in genetic improvement of salt tolerance of rice. Said gene is selected from one of the following nucleotide sequences: 1) the nucleotide sequence from positions 60 to 1649 of SEQ NO: 1 in the Sequence Listing; or 2) a nucleotide sequence that encodes the same protein as that encoded by 1). Transgenic rice plants obtained by introducing into rice the nucleotide sequence comprising OsNHAD gene operably ligated with exogenous promoter had enhanced salt tolerance.
Huazhong Agricultural University | Date: 2010-04-29
A histone deacetylase gene OsHDT1 which enhances utilization of heterosis in rice is isolated and cloned, said gene consisting of: 1) the DNA sequence of positions 1-894 in SEQ ID NO:1 in the Sequence listing; or 2) a DNA sequence which encodes the same protein as that encoded by the DNA sequence of 1). The histone deacetylase gene OsHDT1 is associated with enhanced utilization of heterosis in rice. When the transgenic plants having no phenotype were crossed with Zhenshan 97A, the F1 overexpression hybrid plants have obviously earlier flowering period than F1 wild-type hybrid plants. Moreover, the RNAi inhibition hybrid plants have a decreased seed setting rate compared to the negative control hybrid plants, while the parent RNAi inhibition plants and negative control plants shows no evident difference in the trait of seed setting rate. Western blotting revealed that the gene is capable of deacetylating histone, mainly at H4K16 site. In addition, there exist significant differences in the level of histone modification among Minghui 63, Zhenshan 97 and Shanyou63.
Huazhong Agricultural University | Date: 2013-01-18
A method of co-extracting multiple proteins from chicken egg white is provided. Precipitate proteins step-by-step with polyethylene glycol, then separate the proteins via Q Sepharose Fast Flow anion-exchange chromatography. The method of co-extracting multiple proteins from chicken egg white is capable of obtaining five proteins simultaneously in one extraction process, and the protein products have high purity and high recovery ratio. And the method can be carried out only requiring common-used chromatographic filler, greatly reduces production cost, and provides favorable factors for large-scale industrial production of extracting protein from egg white.