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Huangshi, China

Zhu Z.,Huangshi Love and Health Hospital | Zhang J.,Huangshi Central Hospital | Jiang W.,Huangshi No 5 Hospital | Zhang X.,Jingzhou Central Hospital | And 2 more authors.
OncoTargets and Therapy | Year: 2015

Background: It is known that bladder cancer disease is closely related to aromatic amine compounds, which could cause cancer by regulating of N-acetylation and N-acetyltransferase 1 and 2 (NAT1 and NAT2). The NAT2 slowed acetylation and would increase the risk of bladder cancer, with tobacco smoke being regarded as a risk factor for this increased risk. However, the relationship between NAT2 slow acetylation and bladder cancer is still debatable at present. This study aims to explore preliminarily correlation of NAT2 slow acetylation and the risk of bladder cancer. Methods: The articles were searched from PubMed, Cochran, McGrane English databases, CBM, CNKI, and other databases. The extraction of bladder cancer patients and a control group related with the NAT2 gene were detected by the state, and the referenced articles and publications were also used for data retrieval. Using a random effects model, the model assumes that the studies included in the analysis cases belong to the overall population in the study of random sampling, and considering the variables within and between studies. Data were analyzed using STATA Version 6.0 software, using the META module. According to the inclusion and exclusion criteria of the literature study, 20 independent studies are included in this meta-analysis. Results: The results showed that the individual differences of bladder cancer susceptibility might be part of the metabolism of carcinogens. Slow acetylation status of bladder cancer associated with the pooled odds ratio was 1.31 (95% confidence interval: 1.11–1.55). Conclusion: The status of NAT2 slow N-acetylation is associated with bladder cancer risks, and may increase the risk of bladder cancer. © 2015 Xu et al. Source

Liu W.,Huangshi Central Hospital | Ou J.,Pharmacy
Medical Journal of Wuhan University | Year: 2012

Objective: To prepare and optimize the formula of Minoxidil nanostructured lipid carriers. Methods: A modified method of emulsion evaporation at a high temperature and solidification at a low temperature was used to prepare Minoxidil nanostructured lipid carriers, and the quality was evaluated. The orthogonal experiment was operated to optimize the preparative method of Minoxidil nanostructured lipid carriers, according to four factors: the qualities ratio of stearic acid to lecithine, the lipid ratio of solid to liquid, drug to lipid ratio, and the concentration of emulsifier. And the rate of envelopment was used as evaluation index. Results: While the ratio of stearic acid/soybean lecithin was 7:5, solid/fluid was 1:3, drug/lipid was 1:10, and the concentration of emulsifier was 450 g/L, the optimal prescription of Minoxidil nanostructured lipid carriers was obtained. Namely, each 10 ml Nanostructured lipid carriers need to add minoxidil 50 mg, stearic acid 70 mg, oleic acid 50 mg, octadecanoic 30 mg, soybean lecithin 50 mg and Brij78 450 mg. Using the above formula, the average particle diameter of nanostructured lipid carriers was (58.2±16.5) nm and the entrapment efficiency was (86.89±6.26)%. Conclusion: The ideal Minoxidil nanostructured lipid carriers of high encapsulation efficiency and small particle diameter could be obtained. The optimized formula is feasible and stable. Source

Li K.,Renmin University of China | Yang B.,Renmin University of China | Zhao C.,Huangshi Central Hospital
Journal of Cardiovascular Medicine | Year: 2014

AIMS: Heat shock protein 70 (HSP70) protects against cardiac diseases such as ischemia/reperfusion injury and myocardial infarction. However, the underlying mechanisms have not yet been fully characterized. METHODS: In this study, we investigated the effects of reactive oxygen species (ROS) and transforming growth factor-β-Activated kinase 1 (TAK1) on HSP70-regulated cardiomyocyte protection. Cultured cardiomyocytes of neonatal rats were transfected with HSP70, TAK1 or both of them before exposure to H 2O2, and the ROS generation, p38 mitogen-Activated protein kinase (p38) activity and apoptosis were examined. RESULTS: H2O 2 significantly enhanced intracellular ROS generation and apoptosis as expected, and all these cellular events were greatly abolished by overexpression of HSP70. However, H2O2-induced increments in p38 phosphorylation and cardiac cell apoptosis were largely enhanced by TAK1 overexpression, whereas the similar transfection did not affect the ROS generation in the cardiomyocytes. Moreover, inhibition of H2O 2-increased ROS generation, p38 phosphorylation, and cardiomyocytes apoptosis by overexpression of HSP70 tended to disappear when the cells were cotransfected with TAK1. CONCLUSION: Our data suggest that HSP70 protects cardiomyocytes from apoptosis under oxidative stress through downregulation of intracellular ROS generation and inhibition of p38 phosphorylation. Although TAK1 itself has no effect on intracellular ROS accumulation, it may affect the inhibitory effects of HSP70 on ROS generation, p38 activity and cardiomyocyte injury. © 2014 Italian Federation of Cardiology. Source

Cao Z.,Shanghai JiaoTong University | Fu B.,Huangshi Central Hospital | Deng B.,Shanghai JiaoTong University | Zeng Y.,Shanghai JiaoTong University | And 2 more authors.
Cancer Cell International | Year: 2014

Background: Many studies support that chemokine (C-X-C motif) ligand 1 (CXCL1) regulate tumor epithelial-stromal interactions involving in tumor growth and invasion. However, limited studies have been conducted on the expression and function of the CXCL1 gene in hepatocellular carcinoma (HCC).Methods: The mRNA and protein level expression of CXCL1 was examined in HCC tissues and cell lines. The expression of CXCL1 was correlated with clinicopathological features and follow-up data. Overexpression approaches were used to evaluate the biological functions of CXCL1 by MTT and matrigel invasion assays. Protein expression levels of CXCL1 and P65 were determined by western blot analysis.Results: In this study, we found that CXCL1 expression was markedly upregulated in HCC tissues. Ectopic expression of CXCL1 significantly promoted HCC cells proliferation and invasion. Furthermore, CXCL1 promote cell invasion through NF-kB-dependent pathway. CXCL1 expression in HCC associated with clinical stage (P = 0.034) and distant metastasis (P = 0.028). Moreover, Patients with high CXCL1 expression level had poorer overall survival (OS;P = 0.027) than those with low CXCL1 expression.Conclusions: These data indicated that the CXCL1 upregulation may contribute to both the development and progression of HCC and this effect may be associated with increased proliferation and invasiveness mainly via regulating P65 expression. © 2014 Cao et al.; licensee BioMed Central Ltd. Source

Kai L.,Jingmen No. 1 Peoples Hospital | Jia L.,Huangshi Central Hospital | Zhi-Gang W.,Jingmen No. 1 Peoples Hospital | Lei Y.,Huangshi Central Hospital
Indian Journal of Cancer | Year: 2015

Objective: The aim of this retrospective study was to evaluate whether radiofrequency ablation (RFA) combined percutaneous ethanol injection (PEI) in the management of hepatocellular carcinoma (HCC) improves treatment outcomes. Patients and Methods: We retrospectively included 66 HCC patients who received RFA or RFA plus PEI from February 2011 to January 2014 in Jingmen No. 1 People's Hospital. Moreover, 31 cases received RFA plus PEI as the experiment group and 35 subjects treated with RFA aloe as the control group. The overall survival and treatment related complications were compared between the two groups. Results: For RFA group, the 1-year, 2-year, and 3-year survival rate were 82.0%, 69.3%, and 30.7%, respectively, with the median survival time of 27.1 months. For RFA plus PEI group, the 1-year, 2-year, and 3-year survival rate were 97.1%, 73.9%, and 37.5%, respectively, with the median survival time of 33.6 months. The overall survival of the two groups was not statistical different with the hazard ratio of 1.48 (P > 0.05); three cases of treatment associated complications were found in RFA group with 1 abscess, 1 pleural effusion, and 1 portal vein thrombosis. Moreover, 2 cases of complication were recorded in RFA plus PEI group with 1 pleural effusion and 1 portal vein thrombosis. The complicated incidence rate was not statistical different between the two groups (P < 0.05). Conclusion: The combination treatment of HCC was safe and had a slightly higher primary effectiveness rate than RFA alone. Source

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