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Xu H.-W.,Soochow University of China | Ren F.,Soochow University of China | Yu Y.-M.,Huangpu Central Hospital of Shanghai | Cai C.-Z.,Tongji University
Oncology Letters

Accumulating evidence shows that runt-related transcription factor 3 (Runx3) is a putative tumor suppressor in various types of cancer, the lower levels of which are associated with a less favorable cancer outcome. However, these studies were restricted to primary cancer lesions. Lymph node metastasis (LNM) is a significant factor in determining the prognosis of patients with gastric cancer and is a frequent target of chemotherapy. In the present study, we investigated the expression of Runx3 in the lymph nodes (LNs) of stomach carcinoma and the association of Runx3 expression with the prognosis of patients. The expression of Runx3 in LNs with and without metastasis was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting. The positive rate of Runx3 mRNA in LNM specimens was significantly lower (28.4%, 21 out of 74) compared to that of the non-metastatic samples (33.3%, 9 out of 27, P<0.05). Similar findings were obtained by Western blotting. Univariate analysis revealed that the loss of Runx3 expression in LNs was not only associated with poor clinicopathological factors, such as LNM, distant organ metastasis, later clinicopathological stages and deep infiltration, but also with a lower 5-year survival rate and poorer prognosis. These results strongly suggest a potential diagnostic value of Runx3 expression in LNs and multiple pathways contributing to the outcome of patients with gastric cancer. Source

Several genome-wide association studies on breast cancer (BC) have reported similar findings of a new susceptibility locus, 5p12. After that, a number of studies reported that the rs10941679, rs4415084, and rs981782 polymorphism in chromosome 5p12 has been implicated in BC risk. However, the studies have yielded contradictory results. To derive a more precise estimation of the relationship, a meta-analysis of 131,983 BC cases and 200,314 controls from 24 published case-control studies was performed. Overall, significantly elevated BC risk was associated with rs10941679, rs4415084, and rs981782 risk allele when all studies were pooled into the meta-analysis. In the subgroup analysis by ethnicity, significantly increased risks were found for the rs10941679 and rs4415084 polymorphism among Caucasians and East Asians, while no significant associations were observed for the two polymorphisms in African and other ethnic populations. For 5p12-rs981782, significant associations were only detected among Caucasians. In addition, we found that rs10941679 and rs4415084 on 5p12 confer risk, exclusively for estrogen receptor (ER)-positive tumors with per-allele OR of 1.16 (95% CI: 1.11-1.21; P<10(-5)) and of 1.14 (95% CI: 1.09-1.19; P<10(-5)) respectively. Ethnicity was identified as a potential source of between-study heterogeneity. In conclusion, this meta-analysis demonstrated that common variations are a risk factor associated with increased BC susceptibility, but these associations vary in different ethnic populations. Source

Wang H.,Fudan University | Song K.,Fudan University | Chen Z.,Fudan University | Yu Y.,Huangpu Central Hospital of Shanghai

Background:CYP2C19 encodes a member of the cytochrome P450 superfamily of enzymes, which play a central role in activating and detoxifying many carcinogens and endogenous compounds thought to be involved in the development of cancer. In the past decade, two common polymorphisms among CYP2C19 (CYP2C19*2 and CYP2C19*3) that are responsible for the poor metabolizers (PMs) phenotype in humans and cancer susceptibility have been investigated extensively; however, these studies have yielded contradictory results.Methods and Results:To investigate this inconsistency, we conducted a comprehensive meta-analysis of 11,554 cases and 16,592 controls from 30 case-control studies. Overall, the odds ratio (OR) of cancer was 1.52 [95% confidence interval (CI): 1.23-1.88, P<10-4] for CYP2C19 PMs genotypes. However, this significant association vanished when the analyses were restricted to 5 larger studies (no. of cases ≥ 500 cases). In the subgroup analysis for different cancer types, PMs genotypes had an effect of increasing the risks of esophagus cancer, gastric cancer, lung cancer and hepatocellular carcinoma as well as head neck cancer. Significant results were found in Asian populations when stratifi{ligature}ed by ethnicity; whereas no significant associations were found among Caucasians. Stratifi{ligature}ed analyses according to source of controls, significant associations were found only in hospital base controls.Conclusions:Our meta-analysis suggests that the CYP2C19 PMs genotypes most likely contributes to cancer susceptibility, particularly in the Asian populations. © 2013 Wang et al. Source

Yu Y.-M.,Huangpu Central Hospital of Shanghai | Lv J.,Huangpu Central Hospital of Shanghai | Wang C.,Huangpu Central Hospital of Shanghai | Xu M.,Huangpu Central Hospital of Shanghai | Bao J.-L.,Huangpu Central Hospital of Shanghai
Journal of Shanghai Jiaotong University (Medical Science)

Objective: To investigate the ultrasonic imaging and pathological features of encapsulated papillary carcinoma and ductal carcinoma in situ of breast. Methods: The clinical data of 7 cases of encapsulated papillary carcinoma and 12 cases of ductal carcinoma in situ were retrospectively analysed, and there were preoperative ultrasonic findings and images for all cases. The pathological features of tumor tissues were observed with HE staining, and the expression of estrogen receptor (ER), progesterone receptor (PR), p53, Ki67, p63 and CerbB-2 protein was detected by immunohistochemical method. Results: The ultrasonic findings of encapsulated papillary carcinoma indicated that the masses were in irregular shape with unclear boundaries; internal echo was mixed echo; in case of no echo, high echo was found with papillae, and rear echo was enhanced; calcification was not found; and blood flow signals were visible around the tumor. The ultrasonic findings of ductal carcinoma in situ indicated that there was mammary duct ectasia; low echo was in the mammary duct ectasia, with irregular shape, unclear boundaries and no enhancement in the rear echo; calcification was found; and blood flow signals were visible around the tumor. Light microscopy with HE staining indicated that in encapsulated papillary carcinoma, fine fibrovascular cores were found, and neoplastic epithelial cells were of low or intermediate nuclear grades surrounded by a fibrous capsule. And in ductal carcinoma in situ, there was neoplastic proliferation cytological atypia of epithelial cells, and fibrovascular cores with no fibrous capsule were found in the nipple type ductal carcinoma in situ. Immunohistochemical detection revealed that in encapsulated papillary carcinoma, there was significantly positive expression of ER and PR and negative or weakly positive expression of p53, Ki67 and CerbB-2 in epithelial cells, and p63 was negative in myoepithelial cell layer within the papillae or at the periphery of the lesion. And in ductal carcinoma in situ, p63 was negative in myoepithelial cells within the papillae, but was positive in the cells at the periphery of the lesion. Conclusion: The unique ultrasonic imaging and pathological features are helpful in the diagnosis of encapsulated papillary carcinoma and ductal carcinoma in situ of breast. Source

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