Huangdao Entry Exit Inspection and Quarantine Bureau

Qingdao, China

Huangdao Entry Exit Inspection and Quarantine Bureau

Qingdao, China
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Wang Y.-Y.,Qingdao University | Chen A.-F.,Qingdao University | Wang H.-Z.,Huangdao Entry Exit Inspection and Quarantine Bureau | Xie L.-Y.,Qingdao University | And 2 more authors.
Aging Male | Year: 2011

Background. Accumulating evidence implicates leukocyte telomere length (LTL) shortening as a potential risk predictor for cardiovascular disease. Arterial stiffness chronicles the cumulative burden of cardiovascular disease risk factors. Therefore, the capacity of LTL to predict arterial stiffness was examined. Methods. A total of 275 unrelated Chinese males: 163 patients with coronary artery disease (CAD) and 112 healthy controls, 40-73 years of age were included in this study. The relative telomere length of leukocytes was determined by a real-time fluorescence quantitative polymerase chain reaction (PCR). Large artery stiffness was measured with carotid-femoral pulse wave velocity (PWV). Results. The relative telomere length (T/S) ratio was significantly shorter in patients with CAD (0.79±0.26) than in control subjects (1.08±0.22) (p < 0.001). The correlation between LTL and PWV in patients with CAD was stronger than that in the controls (r= ?0.467, r 2 = 0.227, p < 0.001 for patients with CAD versus r = ?0.223; r2 = 0.050; p = 0.018 for controls). The loge-transformed T/S ratio was inversely correlated with age (r = ?0.345; p < 0.001), PWV (r = ?0.326; p < 0.001) and C-reactive protein ( r = ?0.133; p = 0.027). Conclusions. The data show an association of leukocyte telomere length shortening with increased arterial stiffness and cardiovascular burden, suggesting that telomere length is a biomarker of large artery elasticity and CAD. Further studies are warranted to study the role of LTL dynamics in the pathogenesis of atherosclerosis. © 2009 Informa UK, Ltd.


PubMed | Qingdao Agricultural University and Huangdao Entry Exit Inspection and Quarantine Bureau
Type: | Journal: Chemosphere | Year: 2015

The aim of this study was to evaluate the potential toxicity of spirotetramat to the earthworm Eisenia fetida in a natural soil environment. Many biochemical markers, viz., superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione S-transferase (GST), cellulase, and malondialdehyde (MDA) contents were measured after exposure to 0.25, 1.25, and 2.5mgkg(-1) for 2, 7, 14, 21, and 28days. In addition, the comet assay was performed on earthworm coelomocytes to assess the level of genetic damage. The results demonstrate that the SOD activity and MDA content were significantly stimulated by the highest dose (2.5mgkg(-1)) of spirotetramat for the entire period of exposure. The activities of CAT and POD increased significantly by 2d and 21d, respectively, but the activities of both were significantly inhibited after prolonged exposure (28d). After an initial increase on the 2nd day, the cellulase activity in the high-dose treatment group was significantly inhibited for the entire remaining exposure period. The comet assay results demonstrate that spirotetramat (2.5mgkg(-1)) can induce low and intermediate degrees of DNA damage in earthworm coelomocytes. The results indicate that spirotetramat may pose potential biochemical and genetic toxicity to earthworms (E. fetida), and this information is helpful for understanding the ecological toxicity of spirotetramat on soil invertebrate organisms.


Meng L.,Qingdao Technological University | Chen H.,Qingdao Technological University | Ge T.,Huangdao Entry Exit Inspection and Quarantine Bureau | Yang T.,Qingdao Technological University | Jiao K.,Qingdao Technological University
Journal of Polymer Science, Part A: Polymer Chemistry | Year: 2016

A variety of sulfonated polyaniline-graphene oxide (SPAN-GNO) nanocomposites based on GNO, aniline (ANI) and m-aminobenzenesulfonic acid (ABSA) are prepared via changing the mole ratio of ANI to ABSA for the comparison of DNA sensing behavior. Self-signals of SPAN-GNO are employed for estimating the effect of preparation conditions [component, monomer composition (mole ratio of ANI to ABSA), and reaction time] on DNA immobilization and hybridization detection. Then, we find herein that the mole ratio of ANI to ABSA plays a lead role over other factors on hybridization efficiency. Meanwhile, the parallel experiments using methylene blue as the classic indicator verifies this conclusion. The results show that, by comparison with other mole ratio SPAN-GNO nanocomposites-modified electrodes, the mole ratio (2:3) exhibits the widest dynamic detection range from 1.0 × 10-14 to 1.0 × 10-6 M, as well as the lowest detection limit (3.06 × 10-15 M). © 2016 Wiley Periodicals, Inc.


Wang X.,Qingdao Technological University | Ma W.,Qingdao Technological University | Ge T.,Huangdao Entry Exit Inspection and Quarantine Bureau | Yang T.,Qingdao Technological University | Jiao K.,Qingdao Technological University
Electrochimica Acta | Year: 2016

Polymer inorganic nanosheet composites hold great promise in electrochemical sensing applications by improving the electrochemical performance and increasing the surface area. In this work, a reductively treated thin layer molybdenum disulfide nanosheet-poly(xanthurenic acid) (rTLMoS2-PXa) composite have been facilely prepared through a one-step electrosynthesis procedure. The TLMoS2 was used as building block for the construction of the composite with the PXa coated on it. The composite modified electrode possesses improved electron transfer capability and exhibits good electrochemical sensing performance towards several heterocyclic and aromatic ring compounds (2′-deoxyguanosine-5′-triphosphate trisodium salt, dGTP, bisphenol A, BPA and 2,4,6-trinitrotoluene, TNT), which have good affinity to the electroactive PXa and never or rarely been analyzed by MoS2-based sensing platform. This research provides a new electrochemical sensing platform for simple and sensitive detection of dGTP, BPA and TNT, and further extends the application of MoS2 in the field of electrochemical sensing. © 2015 Elsevier Ltd. All rights reserved.


PubMed | Chinese Academy of Inspection and Quarantine and Huangdao Entry Exit Inspection and Quarantine Bureau
Type: Journal Article | Journal: Parasitology research | Year: 2016

Gastrointestinal nematodes within the subfamily Ostertagiinae (Teladorsagia, Ostertagia, and Marshallagia et al.) are among the most common infections of domesticated livestock. These parasites are of particular interest, as many of the species within this group are of economic importance worldwide. Traditionally, nematode species designations have been based on morphological criteria. However, this group possesses poorly defined species. There is an urgent need to develop a reliable technique that can distinguish species of Ostertagiinae. DNA barcoding has been proved to be a powerful tool to identify species of birds, mammals, and arthropods, but this technique has not yet been examined for identifying species of Ostertagiinae. In this study, a total of 138 mitochondrial DNA (mtDNA) cytochrome c oxidase subunit I (COI) sequences from individuals representing 11 species of Ostertagiinae were acquired by PCR for the first time. The specimens were collected from pastoral area of northern China. Genetic divergence analyses showed that mean interspecific Kimura two-parameter distances of COI (13.61 %) were about four times higher than the mean value of the intraspecific divergence (3.69 %). Then, the performance of the COI to identify species of Ostertagiinae was evaluated by identification success rates using nearest neighbor (NN) and BLASTn. The results indicated that the rates of correct sequence identification for COI were high (>80 %) when using the NN and BLASTn methods. Besides, the deep lineage divergences are detected in Teladorsagia circumcincta. Meanwhile, the analyses also detected no genetic differentiation between some species such as Ostertagia hahurica and Ostertagia buriatica. These results indicate that the traditional status of species within Ostertagiinae should be closely examined based on the molecular data.


PubMed | Qingdao Technological University and Huangdao Entry Exit Inspection and Quarantine Bureau
Type: | Journal: Talanta | Year: 2015

The nanocomposite of molybdenum disulfide (MoS2) and polyaniline (PANI) was prepared through in situ polymerization of aniline on the surface and interlayer of thin-layered MoS2. Owing to the physisorption of aromatic aniline onto the basal plane of MoS2, the electrochemical properties of MoS2/PANI nanocomposite were improved. And a novel electrochemical sensor based on MoS2/PANI nanocomposite was used to determine chloramphenicol by differential pulse voltammetry, exhibiting excellent performance. The detection range was from 110(-7) mol L(-1) to 110(-4) mol L(-1), with a high sensitivity and a low detection limit of 6.910(-8) mol L(-1). In addition, this sensor can be used for the determination of chloramphenicol in real samples.


Wang X.,Qingdao University of Science and Technology | Nan F.,Qingdao University of Science and Technology | Zhao J.,Qingdao University of Science and Technology | Yang T.,Qingdao University of Science and Technology | And 2 more authors.
Biosensors and Bioelectronics | Year: 2014

A label-free and ultrasensitive electrochemical DNA biosensor, based on thin-layer molybdenum disulfide (MoS2) nanosheets sensing platform and differential pulse voltammetry detection, is constructed in this paper. The thin-layer MoS2 nanosheets were prepared via a simple ultrasound exfoliation method from bulk MoS2, which is simpler and no distortion compared with mechanical cleavage and lithium intercalation. Most importantly, this procedure allows the formation of MoS2 with enhanced electrochemical activity. Based on the high electrochemical activity and different affinity toward ssDNA versus dsDNA of the thin-layer MoS2 nanosheets sensing platform, the tlh gene sequence assay can be performed label-freely from 1.0×10-16M to 1.0×10-10M with a detection limit of 1.9×10-17M. Without labeling and the use of amplifiers, the detection method described here not only expands the application of MoS2, but also offers a viable alternative for DNA analysis, which has the priority in sensitivity, simplicity, and costs. Moreover, the proposed sensing platform has good electrocatalytic activity, and can be extended to detect more targets, such as guanine and adenine, which further expands the application of MoS2. © 2014 Elsevier B.V.


Wang X.,Qingdao University of Science and Technology | Wang H.,Qingdao University of Science and Technology | Ge T.,Huangdao Entry Exit Inspection and Quarantine Bureau | Yang T.,Qingdao University of Science and Technology | And 2 more authors.
Journal of Physical Chemistry C | Year: 2015

Recently, functional composites based on chemically modified graphenes (CMGs) and nanostructured conducting polymers have attracted wide interest in the field of electrochemical biosensing. However, comprehensive studies of the effects of various CMGs on the electrochemical properties and biosensing performance of the resulting composites are scarce. In this work, for the first time, we fabricated and deeply evaluated three composites composed of CMGs and sulfonic acid-doped polyaniline nanofiber (namely, CMG-SPAN composites). The CMGs (involving the unreduced form and reduced forms prepared by different reduction routes) were chosen to show the effects of reduction and different preparation routes on the morphologies, electrochemical properties, and DNA biosensing performances of the composites. Notably, the self-redox signals of SPAN in these composites were significantly enhanced and were used for rapid, direct, and label-free DNA detection. Moreover, a preliminary study of the capacitive characteristics of the thermally reduced graphene oxide-SPAN composite was conducted at the end of this work, owing to the potential benefits of the composite in a supercapacitor that were surprisingly observed in this research. The findings of this work will provide useful guides for better understanding of the interaction between CMG and SPAN and for the future development of high-performance functional materials for electrochemical sensors/biosensors and supercapacitors. © 2015 American Chemical Society.


Yang T.,Qingdao University of Science and Technology | Yang R.,Qingdao University of Science and Technology | Chen H.,Qingdao University of Science and Technology | Nan F.,Qingdao University of Science and Technology | And 2 more authors.
ACS Applied Materials and Interfaces | Year: 2015

Recently, easy, green, and low-cost liquild exfoliation of bulk materials to obtain thin-layered nanostructure significantly emerged. In this work, thin-layered molybdenum disulfide (MoS2) nanosheets were fabricated through intercalation of self-doped polyaniline (SPAN) to layer space of bulk MoS2 by ultrasonic exfoliating method to effectively prevent reaggregation of MoS2 nanosheets. The obtained hybrid showed specific surface area, a large number of electroactive species, and open accessible space, accompanied by rich negative charged and special conjugated structure, which was applied to adopt positively charged guanine and adenine, based on their strong π-π∗ interactions and electrostatic adsorption. Also, the SPAN-MoS2 interface exhibited the synergistic effect and good electrocatalytic activity compared with the sole SPAN or MoS2 modified electrode. © 2015 American Chemical Society.


PubMed | Qingdao University of Science and Technology and Huangdao Entry Exit Inspection and Quarantine Bureau
Type: | Journal: Biosensors & bioelectronics | Year: 2014

A label-free and ultrasensitive electrochemical DNA biosensor, based on thin-layer molybdenum disulfide (MoS2) nanosheets sensing platform and differential pulse voltammetry detection, is constructed in this paper. The thin-layer MoS2 nanosheets were prepared via a simple ultrasound exfoliation method from bulk MoS2, which is simpler and no distortion compared with mechanical cleavage and lithium intercalation. Most importantly, this procedure allows the formation of MoS2 with enhanced electrochemical activity. Based on the high electrochemical activity and different affinity toward ssDNA versus dsDNA of the thin-layer MoS2 nanosheets sensing platform, the tlh gene sequence assay can be performed label-freely from 1.0 10(-16)M to 1.0 10(-10)M with a detection limit of 1.9 10(-17)M. Without labeling and the use of amplifiers, the detection method described here not only expands the application of MoS2, but also offers a viable alternative for DNA analysis, which has the priority in sensitivity, simplicity, and costs. Moreover, the proposed sensing platform has good electrocatalytic activity, and can be extended to detect more targets, such as guanine and adenine, which further expands the application of MoS2.

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