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Yin H.-Y.,Huaiyin Hospital of Huaian City | Wei J.,Nan Chen Set Branch of Huaiyin Hospital of Huaian City
Journal of Practical Oncology | Year: 2014

Objective: To investigate the differential gene expression profile of paclitaxel (taxol)-resistant human lung adenocarcinoma A549 cells.Methods: The effect of taxol on human lung adenocarcinoma A549 cells and drug-resistant A549/taxol cells was determined by Kaplan-Meier survival curve. The differential gene expression of A549 and A549/taxol cells was examined by microarray assay.Results: The Kaplan-Meier curve showed that mice injected with A549 cells lived 25 days longer than the group injected with A549/taxol cells with the same taxol-based program(P<0.01). Genome-wide expression in these cells revealed that overall 163 and 115 genes were upregulated or downregulated >3 folds, respectively, in A549/taxol cells.Conclusion: The difference in genomic expression may be one of the mechanisms of taxol resistance in A549/taxol cells. Source

Objective: To study the correlation between microRNA (miRNA) polymorphism and the risk and clinical prognosis of acute radiation esophagitis in patients with esophageal neoplasms. Methods: 256 patients with acute radiation esophagitis during radiotherapy were chosen as the experimental group, and 256 patients matched by age and sex without acute radiation esophagitis during radiotherapy were chosen as the control group. The polymorphism types of miRNA-146a (rs29lOl64) were determined by Taqman gene typing technology of ABI7900HT. The genotype distribution of miRNA-146a rs2910164 polymorphism in the experimental and control groups was analyzed. Logistic regression was performed to estimate the odds ratio (OR) and 95 % confidence interval (95 % CI). Results: The genotype frequencies of CC, GG and CG at miRNA-146a polymorphic site rs2910164 in the experiment and control group were 20.70 % (53/256) and 33.20 % (85/256), 45.32 % (116/256) and 40.63 % (104/256), 33.98 % (87/256) and 26.17 % (67/256), respectively. There was a statistically significant difference between two groups (all P < 0.05). Compared with gene type CC, the OR values of acute radiation esophagitis in patients with gene type GC and GG were 0.654 and 0.627, respectively (P < 0.05), indicating that they had a low risk. The negative effect rates in patients with gene type GG, CG and CC were 7.69 % (8/104), 19.40 % (13/67) and 41.18 % (35/85), respectively. There was a statistically significant difference in the clinical prognosis among these genotypes (P < 0.05). Conclusion: Gene type CC at miRNA-146a polymorphic site rs2910164 can increase the risk of acute radiation esophagitis and decrease the clinical prognosis in patients with esophageal neoplasms. © Copyright 2016 by the Chinese Medical Association. Source

Yang W.,Nanjing Medical University | Tian R.,Air Force General Hospital of PLA | Xue T.,Huaiyin Hospital of Huaian City
Tumor Biology | Year: 2015

We evaluated whether degrees of dysplasia may be consistently accessed in an automatic fashion, using different kinds of non-melanoma skin cancer (NMSC) as a validatory model. Namely, we compared Bowen disease, actinic keratosis, basal cell carcinoma, low-grade squamous cell carcinoma, and invasive squamous cell carcinoma. We hypothesized that characterizing the shape of nuclei may be important to consistently diagnose the aggressiveness of a skin tumor. While basal cell carcinoma is comparatively relatively benign, management of squamous cell carcinoma is controversial because of its potential to recur and intraoperative dilemma regarding choice of the margin or the depth for the excision. We provide evidence here that progressive nuclear dysplasia may be automatically estimated through the thresholded images of skin cancer and quantitative parameters estimated to provide a quasi-quantitative data, which can thenceforth guide the management of the particular cancer. For circularity, averaging more than 2500 nuclei in each group estimated the means ± SD as 0.8 ± 0.007 vs. 0.78 ± 0.0063 vs. 0.42 ± 0.014 vs. 0.63 ± 0.02 vs. 0.51 ± 0.02 (F = 318063.56, p < 0.0001, one-way analyses of variance). The mean aspect ratios were (means ± SD) 0.97 ± 0.0014 vs. 0.95 ± 0.002 vs. 0.38 ± 0.018 vs. 0.84 ± 0.0035 vs. 0.74 ± 0.019 (F = 1022631.931, p < 0.0001, one-way analyses of variance). The Feret diameters averaged over 2500 nuclei in each group were the following: 1 ± 0.0001 vs. 0.9 ± 0.002 vs. 5 ± 0.031 vs. 1.5 ± 0.01 vs. 1.9 ± 0.004 (F = 33105614.194, p < 0.0001, one-way analyses of variance). Multivariate analyses of composite parameters potentially detect aggressive variants of squamous cell carcinoma as the most dysplastic form, in comparison to locally occurring squamous cell carcinoma and basal cell carcinoma, or benign skin lesions. © 2015, International Society of Oncology and BioMarkers (ISOBM). Source

Zhou Z.,Huaiyin Hospital of Huaian City | Luo C.,Guizhou Orthopedics Hospital | Li X.,Soochow University of China | Zhang Y.,Huaian First Peoples Hospital | And 3 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2016

This study aimed to analyze the indications and outcomes of surgical treatment of complex cervical spondylotic myelopathy (CSM) by a single-stage combined anterior and posterior approach. Eighteen patients with CSM were operated between March 2007 and October 2012 by using the single-stage combined anterior and posterior approach to achieve decompression of the spinal cord. Patients’ clinical and radiological data were analyzed to evaluate the clinical efficacy of the procedure. The mean operation time was 3.2 hours (range, 2.5-5 hours). Mean intraoperative blood loss was 650 mL (range, 480-1000 mL). Four cases had cerebrospinal fluid leakage during the surgery. Patients were followed-up for a mean duration of 38 months (range, 18 months to 7 years). The rates of neurological improvement and eligible rate of nerve function were 78.1% and 67.4%, respectively. Magnetic resonance imaging demonstrated repositioning of the dura mater posteriorly to its expected position and confirmed achievement of anterior and posterior decompression of the spinal cord. Computed tomography demonstrated a fully expanded spinal canal, and an average laminar opening angle of 45.2°. Our results demonstrated that single-stage surgical treatment of CSM by a combined anterior and posterior approach successfully achieved complete spinal cord decompression and was associated with good mid-term clinical efficacy. © 2016, E-Century Publishing Corporation. All rights reserved. Source

Li X.,Soochow University of China | Zhou Z.-Y.,Huaiyin Hospital of Huaian City | Zhang Y.-Y.,Huaian First Peoples Hospital | Yang H.-L.,Soochow University of China
PLoS ONE | Year: 2016

Osteoporosis is one of the most prevalent skeletal system diseases. It is characterized by a decrease in bone mass and microarchitectural changes in bone tissue that lead to an attenuation of bone resistance and susceptibility to fracture. Vertebral fracture is by far the most prevalent osteoporotic fracture. In the musculoskeletal system, osteoblasts, originated from bone marrow stromal cells (BMSC), are responsible for osteoid synthesis and mineralization. In osteoporosis, BMSC osteogenic differentiation is defective. However, to date, what leads to the defective BMSC osteogenesis in osteoporosis remains an open question. In the current study, we made attempts to answer this question. A mouse model of glucocorticoid-induced osteoporosis (GIO) was established and BMSC were isolated from vertebral body. The impairment of osteogenesis was observed in BMSC of osteoporotic vertebral body. The expression profiles of thirty-six factors, which play important roles in bone metabolisms, were compared through antibody array between normal and osteoporotic BMSC. Significantly higher secretion level of IL-6 was observed in osteoporotic BMSCs compared with normal control. We provided evidences that IL-6 over-secretion impaired osteogenesis of osteoporotic BMSC. Further, it was observed that β-catenin activity was inhibited in response to IL-6 over-secretion. More importantly, in vivo administration of IL-6 neutralizing antibody was found to be helpful to rescue the osteoporotic phenotype of mouse vertebral body. Our study provides a deeper insight into the pathophysiology of osteoporosis and identifies IL-6 as a promising target for osteoporosis therapy. © 2016 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Source

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