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Xin Y.,Hunan University | Xin Y.,Huaihua College | Fu-Bing X.,Hunan University | Hong-Wei L.,Huaihua College | And 3 more authors.
Electrochimica Acta | Year: 2013

A disposable biosensor for determination of hydrogen peroxide (H 2O2) based on Fe3O4-Au magneticnanoparticles coated horseradish peroxidase (HRP) and graphene sheets (GS)-Nafion film modifiedscreen-printed carbon electrode (SPCE) was fabricated. To construct the H2O2biosensor, GS-Nafion solu-tion was first dropped onto the surface of SPCE. Subsequently, the biocomposites of Fe3O4-Au magneticnanoparticles coated HRP were adsorbed on the surface with the aid of an external magnetic field tofabricate the SPCE|GS-Nafion/Fe3O4-Au-HRP electrode. X-ray powder diffractometer (XRD), scanningelectron microscopy (SEM), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) andchronoamperometry(i-t curve) were employed to study the synthesis of GS, the construction processesand electrochemical properties of the biosensor. Under optimized experimental conditions, CV demon-strated that the direct electron transfer (DET) of HRP was realized. The biosensor had an excellentperformance in terms of electrocatalytic reduction toward H2O2. The linear response of the biosensor to H2O2 was in the concentration range of 2.0 × 10-5 mol/L to 2.5 × 10-3mol/L (R = 0.9994) with a detectionlimit of 1.2 × 10-5mol/L (S/N = 3). The proposed electrochemical biosensor was sensitive, rapid, dispos-able with low cost, fewer sample volume, easy preparation and strong anti-interference, which showed great promise for screen-determination of trace H2O2in real samples. © 2013 Elsevier Ltd. All rights reserved.

Yang X.,Huaihua College | Yang X.,Hunan University | Wu F.,Huaihua College | Chen D.-Z.,Huaihua College | Lin H.-W.,Huaihua College
Sensors and Actuators, B: Chemical | Year: 2014

A novel magnetic field controllable and disposable electrochemical immunosensor for rapid determination of clenbuterol (CLB) was fabricated by graphene sheets (GS)-Nafion (Nf) film dropped on the screen-printed carbon electrode (SPCE) and then Fe3O4-Au nanoparticles (GoldMag particles, GMP) coated bovine serum albumin-CLB (BSA-CLB) conjugates were absorbed on it with the aid of external magnetic field. X-ray powder diffractometer (XRD), electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM) were employed to characterize the synthesized GS and the construction processes of the modified electrode. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used to study its electrochemical properties. The content of CLB was determined with a competitive immunoassay mode. When different concentration of CLB and 2.0 μg/mL anti-CLB were added to the phosphate buffer solution (PBS) containing 2 mmol/L K3[Fe(CN) 6], the percentage of anodic peak DPV current increase ratio (CI%) was proportional to the concentration of CLB over the range of 0.5 ng/mL to 200.0 ng/mL after incubation for 15 min at 35 C. The detection limit was 0.22 ng/mL. The immunosensor was employed to determine CLB in pork samples and the results were consistent with high-performance liquid chromatography (HPLC) method. The proposed electrochemical immunosensor is sensitive, rapid, magnetic field controllable, low sample consumable and disposable, which is suitable for determining trace CLB in real samples. © 2013 Elsevier B.V.

Li A.-M.,CAS South China Botanical Garden | Li A.-M.,Huaihua College | Wu X.-Q.,CAS South China Botanical Garden | Zhang D.-X.,CAS South China Botanical Garden | Barrett S.C.H.,University of Toronto
Annals of Botany | Year: 2010

Background and Aims: Evolutionary transitions from heterostyly to dioecy have been proposed in several angiosperm families, particularly in Rubiaceae. These transitions involve the spread of male and female sterility mutations resulting in modifications to the gender of ancestral hermaphrodites. Despite sustained interest in the gender strategies of plants, the structural and developmental bases for transitions in sexual systems are poorly understood. Methods: Here, floral morphology, patterns of fertility, pollen-tube growth and floral development are investigated in two populations of the scandent shrub Mussaenda pubescens (Rubiaceae), native to southern China, by means of experimental and open-pollinations, light microscopy, fluorescence microscopy and scanning electron microscopy combined with paraffin sectioning. Key Results Mussaenda pubescens: has perfect (hermaphroditic) flowers and populations with two style-length morphs but only weak differentiation in anther position (stigma-height dimorphism). Experimental pollinations demonstrated that despite morphological hermaphroditism, the species is functionally dioecious. The long-styled (L) morph possesses sterile pollen and functions as a female, whereas the short-styled (S) morph is female sterile and functions as a male. Self- and intra-morph pollinations of the S-morph were consistent with those expected from dimorphic incompatibility. The two populations investigated were both S-morph (male) biased. Investigations of early stages of floral development indicated patterns typical of hermaphroditic flowers, with no significant differences in organ growth between the floral morphs. Meiosis of microspore mother cells was of the simultaneous type with tetrads isobilateral in shape. The tapetal cells in anther walls of the L-morph became vacuolized during meiosis I, ahead of the uninucleate microspore stage in the S-morph. In the L-morph, the microspore nucleus degenerated at the tetrad stage resulting in male sterility. Microsporogenesis and male gametophyte development was normal in the S-morph. Failure in the formation of megaspore mother cells and/or the development of megagametophytes resulted in female sterility in the S-morph, compared with normal megasporogenesis in the L-morph. Conclusions In M. pubescens: cryptic dioecy has evolved from stigma-height dimorphism as a result of morph-specific sterility mutations. © The Author 2010. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved.

Li S.,Huaihua College | Hu L.,Chongqing University
2015 International Conference on Logistics, Informatics and Service Science, LISS 2015 | Year: 2015

In recent years, The vulnerability of agricultural products chain is been exposed because of the endlessly insecure events appeared in every areas and every degrees from the natural disasters on the each node operation of agricultural products supply chain in recently years. As an very important place of HUNAN Province because of its abundant agricultural products, the Eastern Area's security in agricultural products supply chain was related to the safety and stability of economic development in the entire region. In order to make the more objective, scientific, practical of risk management in the empirical analysis, This item is based on the AHP-FCS method to deal with the qualitative to quantitative analysis about risk management of agricultural product supply chain, to identify and evaluate the probability and severity of all the risk possibility. © 2015 IEEE.

Li J.-C.,National University of Defense Technology | Huang B.,Huaihua College | Peng Y.-X.,National University of Defense Technology
Wuli Xuebao/Acta Physica Sinica | Year: 2012

Bilateral filtering can effectively smooth synthetic aperture radar (SAR) images with preserving the edges, but it is difficult to configure the parameters of bilateral filtering to the optimum. The iterative method to configure the parameters with high precision and efficiency has been presented, but the iterative process may be aborted incorrectly. In this paper, we present a modified configuration method to get the optimal trade-off parameters, and give the proof of the convergence of the method. The experimental results on real SAR images show that the modified method can not only obtain equivalent visibility as the iterative method, but also higher accuracy of the gray value similarity variance than that under identical iterative precision. With the iterative precision improved, the convergence rate of the modified method is faster than that of the iterative method. © Chinese Physical Society.

Wei G.,Huaihua College | Shen J.,Hangzhou Normal University
Computers and Mathematics with Applications | Year: 2010

This paper is concerned with the nonlinear delay difference equations with positive and negative coefficients x(n+1)-x(n)+p(n)f(x(n-k))-q(n)f(x(n-l))=0, n

Yuan Y.,Huaihua College
Proceedings of the International Conference on E-Business and E-Government, ICEE 2010 | Year: 2010

Substitution effect of tourism product is the phenomenon that some tourism products substitute each others because of their similar value and utility. With the goal to appraise the substitution effect of tourism product quantitatively, the author utilizes the multi-level grey theory which is the synthesis of grey correlation theories and group-decision analytical hierarchy process (AHP). The author also carries out the demonstration study of Wuling and Red Stone Forest, which indicates: there is certain substitution effect between the geological tourism resources groups of Wuling and Red Stone Forest, but Wuling silicarenite resources group has no sufficiently strong substitution effect for Red Stone Forest, so the Wuling Geological tourism resources group has the shielding effect on the Red Stone Forest. Through demonstration study, it is confirmed that application of multi-level grey theory in appraising quantitatively the substitution effect of tourism product is objective and credible, avoiding the disadvantages of analytic hierarchy process. This method provides a feasible quantitative analysis tool for the substitution effect appraisal with small sample and poor information. © 2010 IEEE.

Yuan Y.,Huaihua College
2011 International Conference on Management Science and Industrial Engineering, MSIE 2011 | Year: 2011

As the capacity of tourism resources to develop tourism industry and to gain economic, social and environmental benefits, the potential of tourism development is the application prospect of tourism development conditions, evaluation of which can help tourism managers to make reasonable decision of tourism development. Multi-level grey approach is the combination of the analytic hierarchy process and the grey system theory, which is applied to carry on researches with quite small sample and poor information. In this work, multi-level grey approach is applied to evaluate the development potential of Langshan Danxia scenic area, and the result indicates that it belongs to more-high scope. So the conclusion is that high-grade tourist resources are not equal to the good development potential. To strengthen the tourism development potential of Langshan Danxia scenic area, the tourism managers should not only develop the new product and new product function, but also develop the new tourist market unceasingly. ©2011 IEEE.

Wei G.,Huaihua College
Tamkang Journal of Mathematics | Year: 2014

This paper is concerned with the nonlinear neutral delay differential equation with positive and negative coefficients [x(t )c(t )x(t φ)]′ +p(t ) f (x(t δ))q(t ) f (x(t σ)) = 0, t ≥ t0, where φ ε (0,∞), δ and σ ε [0,∞), c(t ) ε C([t0,σ),R), p(t) and q(t) ε C([t0,σ), [0, σ)), f ε C(R,R). Sufficient conditions are obtained under which every solution of the above equation is bounded and tends to a constant as t → σ. Our results extend and improve some known results.

Lin X.,Huaihua College | Tang X.,Central South University
Computers and Mathematics with Applications | Year: 2015

We consider the semilinear Schrödinger equation {-Δu+V(x)u=f(x,u),x εRN,u εH1(RN), where V(x) is asymptotically periodic and sign-changing, f(x,u) is asymptotically periodic in x, and asymptotically linear as |u|→ ∞. Under some weak assumptions on V and f, we prove that the above problem has a nontrivial solution. © 2015 Elsevier Ltd. All rights reserved.

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