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Jiang L.-F.,Huaihai Institute of Technology
International Journal of Biological Macromolecules | Year: 2014

In this study, investigated was the effect of the porphyra yezoensis polysaccharides (PPs) on the denaturation of bighead carp (Aristichthys nobilis) myofibrillar protein (Mf) during frozen storage at -18. °C for 90. d. The PPs (2.5%, 5%, and 7.5%, respectively) was added to 100. g of Mf. The changes in the Ca-adenylpyrophosphatase (ATPase) activity and unfrozen water content in Mf were examined to evaluate he denaturation of Mf during frozen storage. Ca-ATPase activity decreased gradually during frozen storage at -18. °C upon addition of PPs. By contrast, Ca-ATPase activity in the control group dropped drastically during the first 45. d of storage and then further decreased gradually for up to 90. d of storage, indicating a biphasic denaturation pattern. PPs addition significantly increased sulfhydryl contents in the Mf of the treatment groups compared with that of the control group (p< 0.05) during frozen storage at -18. °C. © 2014 Elsevier B.V. Source

In this study, response surface methodology was used for optimization the fermentation conditions for pullulan production by the strain Aureobasidium pullulans SK1002 in shaking flask cultures. The production of pullulan was significantly affected by temperature, fermentation time and initial pH. The optimal cultivation conditions stimulating the maximal pullulan production were as follows: temperature, 28 °C; fermentation time, 5 days and initial pH, 5.5. Under these optimized conditions, the predicted maximal pullulan yield was 30.28 g/L. The application of response surface methodology resulted in a significant enhancement in pullulan production. Results of these experiments indicated that response surface methodology was a promising method for optimization of pullulan production. © 2009 Elsevier Ltd. All rights reserved. Source

Wu S.-J.,Huaihai Institute of Technology
Carbohydrate Polymers | Year: 2014

Hydrogen peroxide (H2O2) can degrade polysaccharides and has bleaching effect. In this study, the oligosaccharides derived from Laminaria japonica were prepared by hydrolysis with H2O2 and their antioxidant activity was investigated. The optimal hydrolysis conditions were determined as follows: reaction time 24 h, reaction temperature 75 C, and H2O2 concentration 4%. Under the optimum conditions, the maximum yield of the oligosaccharides reached 17.65%, which was higher than that of aqueous extraction, and at the same time, the maximal decoloration rate reached 79.85%. The oligosaccharides sample contained 94.82% sugar, of which the average degree was approximately 8, and showed light green. The oligosaccharides derived from L. japonica showed high hydroxyl radical scavenging activity (91.31%) at the concentration of 100 μg/mL. © 2014 Elsevier Ltd. Source

Wu S.,Huaihai Institute of Technology
International Journal of Biological Macromolecules | Year: 2014

Chitosan and chitooligosaccharides are preservatives with proven antibacterial activity, while glutathione has antioxidant activity. This study investigated the effects of chitosan coating combined with chitooligosaccharides and glutathione (0.8% glutathione + 1% chitooligosaccharides + 1% chitosan) on preservation of white shrimp (Penaeus vannamei) during partially frozen storage. Chitosan-based coating treatments effectively inhibited bacterial growth, reduced total volatile basic nitrogen and malondialdehyde, and basically maintained the sensory properties of white shrimp (P. vannamei) during partially frozen storage. Therefore, chitosan-based edible coating combined with chitooligosaccharides and glutathione could be a promising antimicrobial and oxidant method to prevent metamorphism of white shrimp with extended shelf life. © 2014 Elsevier B.V. Source

Jiang L.,Huaihai Institute of Technology
International Journal of Biological Macromolecules | Year: 2013

This study aims to investigate the effect of nitrogen source on curdlan production by Alcaligenes faecalis ATCC 31749. Curdlan production fell when excess nitrogen source was present, while biomass accumulation increased as the level of nitrogen source raised. Curdlan production and biomass accumulation were greater with urea compared with those with other nitrogen sources. The highest production of curdlan and biomass accumulation by A. faecalis ATCC 31749 was 28.16gL-1 and 9.58gL-1, respectively, with urea, whereas those with NH4Cl were 15.17gL-1 and 6.25gL-1, respectively. The optimum fermentation time for curdlan production was also affected by the nitrogen source in the medium. © 2012 Elsevier B.V. Source

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