Huaihai Institute of Technology

www.hhit.edu.cn
Xinpu, China

Time filter

Source Type

Wang X.S.,Huaihai Institute of Technology
Journal of Applied Geophysics | Year: 2013

The concentrations of four selected heavy metals (Pb, Zn, Ni and Cr) were measured on 167 topsoil samples collected from the city of Xuzhou, China via inductively coupled plasma mass spectroscopy (ICP-MS). It was found that Pb and Zn were principally derived from anthropogenic inputs whereas Cr and Ni distributions were mainly controlled by parent materials. The spatial distribution patterns of Pb, and Zn were similar to that from low field magnetic susceptibility (χlf), suggesting interaction among them. Two threshold values for magnetic susceptibility and frequency-dependent susceptibility percentage (χfd) were applied to discriminate between polluted and unpolluted samples according to their magnetic susceptibility, resulting in 109 samples populating the "polluted" subset. The Pb and Zn concentrations of the "polluted" subset were statistically significantly higher than those measured in the "unpolluted" one. The heavy metal concentrations were also investigated varying the magnetic susceptibility thresholds to change the "polluted" subset. © 2013 Elsevier B.V.


Wu S.,Huaihai Institute of Technology
International Journal of Biological Macromolecules | Year: 2014

Chitosan and chitooligosaccharides are preservatives with proven antibacterial activity, while glutathione has antioxidant activity. This study investigated the effects of chitosan coating combined with chitooligosaccharides and glutathione (0.8% glutathione + 1% chitooligosaccharides + 1% chitosan) on preservation of white shrimp (Penaeus vannamei) during partially frozen storage. Chitosan-based coating treatments effectively inhibited bacterial growth, reduced total volatile basic nitrogen and malondialdehyde, and basically maintained the sensory properties of white shrimp (P. vannamei) during partially frozen storage. Therefore, chitosan-based edible coating combined with chitooligosaccharides and glutathione could be a promising antimicrobial and oxidant method to prevent metamorphism of white shrimp with extended shelf life. © 2014 Elsevier B.V.


Wu S.-J.,Huaihai Institute of Technology
Carbohydrate Polymers | Year: 2014

Hydrogen peroxide (H2O2) can degrade polysaccharides and has bleaching effect. In this study, the oligosaccharides derived from Laminaria japonica were prepared by hydrolysis with H2O2 and their antioxidant activity was investigated. The optimal hydrolysis conditions were determined as follows: reaction time 24 h, reaction temperature 75 C, and H2O2 concentration 4%. Under the optimum conditions, the maximum yield of the oligosaccharides reached 17.65%, which was higher than that of aqueous extraction, and at the same time, the maximal decoloration rate reached 79.85%. The oligosaccharides sample contained 94.82% sugar, of which the average degree was approximately 8, and showed light green. The oligosaccharides derived from L. japonica showed high hydroxyl radical scavenging activity (91.31%) at the concentration of 100 μg/mL. © 2014 Elsevier Ltd.


Jiang L.-F.,Huaihai Institute of Technology
International Journal of Biological Macromolecules | Year: 2014

In this study, investigated was the effect of the porphyra yezoensis polysaccharides (PPs) on the denaturation of bighead carp (Aristichthys nobilis) myofibrillar protein (Mf) during frozen storage at -18. °C for 90. d. The PPs (2.5%, 5%, and 7.5%, respectively) was added to 100. g of Mf. The changes in the Ca-adenylpyrophosphatase (ATPase) activity and unfrozen water content in Mf were examined to evaluate he denaturation of Mf during frozen storage. Ca-ATPase activity decreased gradually during frozen storage at -18. °C upon addition of PPs. By contrast, Ca-ATPase activity in the control group dropped drastically during the first 45. d of storage and then further decreased gradually for up to 90. d of storage, indicating a biphasic denaturation pattern. PPs addition significantly increased sulfhydryl contents in the Mf of the treatment groups compared with that of the control group (p< 0.05) during frozen storage at -18. °C. © 2014 Elsevier B.V.


In this study, response surface methodology was used for optimization the fermentation conditions for pullulan production by the strain Aureobasidium pullulans SK1002 in shaking flask cultures. The production of pullulan was significantly affected by temperature, fermentation time and initial pH. The optimal cultivation conditions stimulating the maximal pullulan production were as follows: temperature, 28 °C; fermentation time, 5 days and initial pH, 5.5. Under these optimized conditions, the predicted maximal pullulan yield was 30.28 g/L. The application of response surface methodology resulted in a significant enhancement in pullulan production. Results of these experiments indicated that response surface methodology was a promising method for optimization of pullulan production. © 2009 Elsevier Ltd. All rights reserved.


Jiang L.,Huaihai Institute of Technology
International Journal of Biological Macromolecules | Year: 2013

This study aims to investigate the effect of nitrogen source on curdlan production by Alcaligenes faecalis ATCC 31749. Curdlan production fell when excess nitrogen source was present, while biomass accumulation increased as the level of nitrogen source raised. Curdlan production and biomass accumulation were greater with urea compared with those with other nitrogen sources. The highest production of curdlan and biomass accumulation by A. faecalis ATCC 31749 was 28.16gL-1 and 9.58gL-1, respectively, with urea, whereas those with NH4Cl were 15.17gL-1 and 6.25gL-1, respectively. The optimum fermentation time for curdlan production was also affected by the nitrogen source in the medium. © 2012 Elsevier B.V.


Water soluble chitosan (WSC) was prepared by hydrolysing chitosan using α-amylase. The degradation was monitored by WSC recovery and the WSC structure was characterised by Fourier transform infrared spectroscopy. The optimal hydrolysis conditions were found to be at pH 5, 50 °C, 20 mg α-amylase in the reaction solution, with a reaction time of 4 h. Under these conditions, the average molecular weight of chitosan decreased to 730 Da. The resulting products were composed of chitooligosaccharides of DP 3-7. The hydrolysate was neutralised with 1 M NaOH, filtered, concentrated to 16% (w/v), precipitated with five volumes of ethanol to one volume of the concentrated hydrolysate solution, and dried at 60 °C for 3 h to yield a white powder. The WSC content of the product and the yield were 96.2% and 91.2% (w/w), respectively. © 2011 Elsevier Ltd. All rights reserved.


Jiang L.-F.,Huaihai Institute of Technology
Carbohydrate Polymers | Year: 2014

In this study, the Lycium barbarum oligosaccharides (LBO) were prepared by hydrolysis using hydrogen peroxide (H2O2). The yield of the LBO was monitored during the hydrolysis process. The hydrolysis conditions were optimized as follows: time, 4 h; temperature, 70 C; and H2O 2 concentration, 2.5% (v/v). The hydrolysates were filtered, concentrated to ∼20% (w/v), precipitated with 6 volumes of absolute ethanol, freeze-dried, and ground to yield a water soluble and white powder. The sugar content of the product was 95.8%, and the yield was 21.05% (w/w), respectively. The LBO show higher hydroxyl radical scavenging activity (86.46%) than Vc (40.96) at the concentration of 100 μg/mL. © 2013 Elsevier Ltd. All rights reserved.


Qian Z.-G.,Huaihai Institute of Technology
Carbohydrate Polymers | Year: 2014

In this study, cellulase-assisted extraction of water soluble polysaccharides from pumpkin (Cucurbita moschata) and their antibacterial activity were investigated. The polysaccharides yield was monitored during the extraction process. The optimum extraction conditions were determined as follows: time, 40 min; temperature, 55 C; pH, 4.5; and cellulase amount, 4000 U/g. The extracts were centrifuged, filtered, proteins removed by Sevag method, concentrated to ∼15% (w/v), precipitated with 5 volumes of absolute ethanol, freeze-dried, and pulverized to yield a water soluble powder of pumpkin polysaccharides (PP). The sugar content of the product was 68.3%, and the yield was 17.34% (w/w), respectively. The PP had high antibacterial activity against Bacillus subtilis, Staphylococcus aureus, and Escherichia coli at the concentration of 100 mg/mL. © 2013 Elsevier Ltd. All rights reserved.


Wang H.-B.,Huaihai Institute of Technology
Carbohydrate Polymers | Year: 2014

In the present study, we investigated the cellulase-assisted extraction and antibacterial activity of water-soluble polysaccharides from the dandelion Taraxacum officinale. The extraction conditions, optimized for improving yield, were as follows: time, 46.11 min; temperature, 54.87 C; pH, 4.51 and cellulase enzyme, 4000 U/g. Under these conditions, the yield of polysaccharides from dandelion (PD) reached 20.67% (w/w). The sugar content of PD was 95.6% (w/w), and it displayed high antibacterial activity at a concentration of 100 mg/mL against Escherichia coli, Bacillus subtilis and Staphylococcus aureus. These results indicate that PD may be a viable option for use as a food preservative. © 2013 Elsevier Ltd.

Loading Huaihai Institute of Technology collaborators
Loading Huaihai Institute of Technology collaborators