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Zhang J.,Beijing Entry Exit Inspection and Quarantine Bureau | Xu M.,Linyi Entry Exit Inspection and Quarantine Bureau | Wang X.,Chinese Academy of Sciences | Wang Y.,China National Accreditation Service for Conformity Assessment | And 6 more authors.
Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology | Year: 2013

To develop a specific, rapid and convenient method based on molecular motor biosensor to detect food-borne rotavirus. A specific probe was encompassed the conservative region of rotavirus's VP7 segment, and a molecular motor detect device was constructed by connecting probes to F0F1-ATPase molecular motor through biotin-streptavidin system. This biosensor's sensitivity was 0.005 ng/mL for rotavirus RNA. Extracted virus RNA was conjugated with the biosensor separately, at the same time ATP was synthesized. By comparing fluorescence intensity, we can detect rotavirus RNA in samples. This method possessed specificity for rotavirus, without any cross-reaction with Hepatitis A virus and noroviris, and it could be accomplished within 1 h. We detected 15 samples using this method and the results were compared with RT-PCR results. This method is sensitive and specific for rotavirus, and it can be used to detect food-borne rotavirus. © 2013 by the Institute of Microbiology, the Chinese Academy of Sciences and the Chinese Society for Microbiology. Source

Feng M.,Huaian Entry Exit Inspection and Quarantine Bureau | Feng M.,Jiangnan University | Yong Q.,Jiangnan University | Wang W.,Jiangnan University | And 3 more authors.
Food and Agricultural Immunology | Year: 2013

A pair of monoclonal antibodies (mAb) from 10 murine hybridomas secreting Escherichia coli O157:H7 (E. coli O157:H7)-specific mAbs were selected for the development of the sandwich ELISA to detect E. coli O157:H7. On the basis of pairwise interaction analysis, mAb-1 was selected as a capture antibody while mAb-6 was used as a detection antibody. The buffer system which provided the greatest difference between the specific E. coli O157:H7-positive antigen and the negative control was chosen. This sandwich ELISA showed good linearity when the concentration of E. coli O157:H7 was in the range of 105-108 cfu/mL, and the sensitivity was 1×104 cfu/mL. With 8-h enrichment of bacteria, this ELISA was found to detect 0.4 cfu/g E. coli O157:H7 in artificially contaminated green tea samples. © 2012 Taylor & Francis. Source

Xing C.,Jiangnan University | Liu L.,Jiangnan University | Song S.,Jiangnan University | Feng M.,Huaian Entry Exit Inspection and Quarantine Bureau | And 2 more authors.
Biosensors and Bioelectronics | Year: 2015

In this paper, we describe the development of a multicomponent lateral-flow assay based on an antibody-antigen reaction for the rapid and simultaneous detection of trace contaminants in water, including a heavy metal, algal toxin, antibiotic, hormone, and pesticide. The representative analytes chosen for the study were lead (Pb(II), microcystin-leucine-arginine (MC-LR), chloramphenicol (CAP), testosterone (T), and chlorothalonil (CTN). Five different antigens were immobilized separately in five test lines on a nitrocellulose membrane. The monoclonal antibodies specifically recognized the corresponding antigens, and there was no cross-reactivity between the antibodies in the detection assay. Samples or standards containing the five analytes were preincubated with the freeze-dried colloidal-gold-labeled monoclonal antibody conjugates to improve the sensitivity of the assay. The results were obtained within 20. min with a paper-based sensor. The cut-off values for the strip test were 4. ng/mL for Pb(II), 1. ng/mL for MC-LR, 0.1. ng/mL for CAP, 5. ng/mL for T, and 5. ng/mL for CTN. The assay was evaluated using spiked water samples, and the accuracy and reproducibility of the results were good. In summary, this lateral-flow device provides an effective and rapid method for the onsite detection of multiple contaminants in water samples, with no treatment or devices required. © 2014 Elsevier B.V. Source

Feng M.,Huaian Entry Exit Inspection and Quarantine Bureau | Feng M.,Jiangnan University | Kong D.,Jiangnan University | Wang W.,Jiangnan University | And 3 more authors.
Sensors (Switzerland) | Year: 2015

A rapid, simple, sensitive, and specific immunochromatographic test strip was developed for the detection of Pantoea stewartii subsp.stewartii (Pss) in corn seed which was soaked overnight and then centrifuged for precipitate re-dissolved as samples. A pair of sensitive monoclonal antibodies for the immunochromatographic test strip was generated by mice immunization and cell fusion. Under optimized conditions, the lower detection limit of the strips for Pss was 1 × 105 cfu/mL both in 0.01 M phosphate buffer solution and corn seed samples, with no cross-reactivity with other common plant pathogens. The developed strip is useful and rapid for the detection of Pss in corn seed samples. © 2015 by the authors; licensee MDPI, Basel, Switzerland. Source

Wang W.,Jiangnan University | Feng M.,Huaian Entry Exit Inspection and Quarantine Bureau | Kong D.,Jiangnan University | Liu L.,Jiangnan University | And 2 more authors.
Food and Agricultural Immunology | Year: 2015

A portable immunochromatographic (IC) strip based on gold-labelled monoclonal antibodies was developed for the rapid detection of Pseudomonas syringae pv. maculicola, a plant pathogen. The IC strip detected 105 colony-forming units CFU/ml P. syringae pv. maculicola in pure culture within 10 min and had good specificity. Compared with sandwich enzyme-linked immunosorbent assay, the strip was equally sensitive; however, it is portable and time-efficient. On broccoli and radish seeds, the IC strips had a sensitivity of 5 × 105 CFU/ml and 106 CFU/ml, respectively. The results revealed that the IC strip could have potential applications for P. syringae pv. maculicola detection in plant seeds. © 2015 Taylor & Francis. Source

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