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Feng M.,Huaian Entry Exit Inspection and Quarantine Bureau | Li H.,Huaian Entry Exit Inspection and Quarantine Bureau | Li H.,Yancheng Institute of Technology | Zhang L.,Yancheng Entry Exit Inspection and Quarantine Bureau | And 5 more authors.
Analytical Sciences | Year: 2016

Novel magnetic molecularly imprinted composites were prepared through a facile method using sulfadimethoxine (SDM) as template. The inorganic magnetic nanoparticles were linked with the organic molecularly imprinted polymer (MIP) through irreversibly covalent bond. So, the resulted composites showed excellent stability and reusability under acidic elution conditions. The magnetic MIP composites showed good selectivity, high binding capacity and excellent kinetics toward SDM. Adopting the magnetic MIP composites as extraction material, an off-line magnetic solid-phase extraction (SPE)/high performance liquid chromatography (HPLC) method was established. The calibration curve was linear in the range of 0.05 - 15 mg kg-1 (r2 = 0.9976). The LOD and LOQ were 0.016 and 0.052 mg kg-1, respectively, while the recoveries were in the range of 89.3 - 107.0%. These novel magnetic MIP composites may become a powerful tool for the extraction of template from complex samples with good efficiency. © The Japan Society for Analytical Chemistry.


PubMed | Yancheng Entry Exit Inspection and Quarantine Bureau, Yancheng Institute of Technology and Huaian Entry Exit Inspection and Quarantine Bureau
Type: | Journal: Journal of chromatography. A | Year: 2016

In this study, a boronate affinity silica stationary phase with enhanced binding properties towards cis-diol compounds was prepared through the combination of surface-initiated atom transfer radical polymerization (SI-ATRP) with a Wulff-type boronate as affinity ligand. The stationary phase showed good hydrophilicity and improved binding strength toward adenosine, with binding constant to be as low as 2.3810


Feng M.,Huaian Entry Exit Inspection and Quarantine Bureau | Feng M.,Jiangnan University | Yong Q.,Jiangnan University | Wang W.,Jiangnan University | And 3 more authors.
Food and Agricultural Immunology | Year: 2013

A pair of monoclonal antibodies (mAb) from 10 murine hybridomas secreting Escherichia coli O157:H7 (E. coli O157:H7)-specific mAbs were selected for the development of the sandwich ELISA to detect E. coli O157:H7. On the basis of pairwise interaction analysis, mAb-1 was selected as a capture antibody while mAb-6 was used as a detection antibody. The buffer system which provided the greatest difference between the specific E. coli O157:H7-positive antigen and the negative control was chosen. This sandwich ELISA showed good linearity when the concentration of E. coli O157:H7 was in the range of 105-108 cfu/mL, and the sensitivity was 1×104 cfu/mL. With 8-h enrichment of bacteria, this ELISA was found to detect 0.4 cfu/g E. coli O157:H7 in artificially contaminated green tea samples. © 2012 Taylor & Francis.


Zhang X.,Jiangnan University | Feng M.,Huaian Entry Exit Inspection and Quarantine Bureau | Liu L.,Jiangnan University | Xing C.,Jiangnan University | And 4 more authors.
International Journal of Food Science and Technology | Year: 2013

An enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody was established to detect aflatoxin B1 (AFB1) in tea. The antibody was prepared from a hybridoma derived by fusing Sp2/0-Ag14 myeloma cells and immunised spleen B cells. The effects from pH, ionic strength, and organic solvents on immunoassay were optimised and the 50% inhibition (IC50) value was 0.057 ± 0.007 ng mL-1. Spiked black and green tea samples at 10, 20 and 50 ng g-1 levels of AFB1 were detected with this proposed ELISA. The recoveries for black tea samples ranged from 68.5% to 117.7% and 73.5 to 114.3% for green tea samples. This immunoassay showed no cross-reactions with other mycotoxin family but good recognition with related aflatoxins. These results indicate that the ELISA assay could be used as a screening method for aflatoxin detection in tea samples. © 2013 Institute of Food Science and Technology.


Xing C.,Jiangnan University | Liu L.,Jiangnan University | Song S.,Jiangnan University | Feng M.,Huaian Entry Exit Inspection and Quarantine Bureau | And 2 more authors.
Biosensors and Bioelectronics | Year: 2015

In this paper, we describe the development of a multicomponent lateral-flow assay based on an antibody-antigen reaction for the rapid and simultaneous detection of trace contaminants in water, including a heavy metal, algal toxin, antibiotic, hormone, and pesticide. The representative analytes chosen for the study were lead (Pb(II), microcystin-leucine-arginine (MC-LR), chloramphenicol (CAP), testosterone (T), and chlorothalonil (CTN). Five different antigens were immobilized separately in five test lines on a nitrocellulose membrane. The monoclonal antibodies specifically recognized the corresponding antigens, and there was no cross-reactivity between the antibodies in the detection assay. Samples or standards containing the five analytes were preincubated with the freeze-dried colloidal-gold-labeled monoclonal antibody conjugates to improve the sensitivity of the assay. The results were obtained within 20. min with a paper-based sensor. The cut-off values for the strip test were 4. ng/mL for Pb(II), 1. ng/mL for MC-LR, 0.1. ng/mL for CAP, 5. ng/mL for T, and 5. ng/mL for CTN. The assay was evaluated using spiked water samples, and the accuracy and reproducibility of the results were good. In summary, this lateral-flow device provides an effective and rapid method for the onsite detection of multiple contaminants in water samples, with no treatment or devices required. © 2014 Elsevier B.V.


Feng M.,Huaian Entry Exit Inspection and Quarantine Bureau | Feng M.,Jiangnan University | Kong D.,Jiangnan University | Wang W.,Jiangnan University | And 3 more authors.
Sensors (Switzerland) | Year: 2015

A rapid, simple, sensitive, and specific immunochromatographic test strip was developed for the detection of Pantoea stewartii subsp.stewartii (Pss) in corn seed which was soaked overnight and then centrifuged for precipitate re-dissolved as samples. A pair of sensitive monoclonal antibodies for the immunochromatographic test strip was generated by mice immunization and cell fusion. Under optimized conditions, the lower detection limit of the strips for Pss was 1 × 105 cfu/mL both in 0.01 M phosphate buffer solution and corn seed samples, with no cross-reactivity with other common plant pathogens. The developed strip is useful and rapid for the detection of Pss in corn seed samples. © 2015 by the authors; licensee MDPI, Basel, Switzerland.


Wang W.,Jiangnan University | Feng M.,Huaian Entry Exit Inspection and Quarantine Bureau | Kong D.,Jiangnan University | Liu L.,Jiangnan University | And 2 more authors.
Food and Agricultural Immunology | Year: 2015

A portable immunochromatographic (IC) strip based on gold-labelled monoclonal antibodies was developed for the rapid detection of Pseudomonas syringae pv. maculicola, a plant pathogen. The IC strip detected 105 colony-forming units CFU/ml P. syringae pv. maculicola in pure culture within 10 min and had good specificity. Compared with sandwich enzyme-linked immunosorbent assay, the strip was equally sensitive; however, it is portable and time-efficient. On broccoli and radish seeds, the IC strips had a sensitivity of 5 × 105 CFU/ml and 106 CFU/ml, respectively. The results revealed that the IC strip could have potential applications for P. syringae pv. maculicola detection in plant seeds. © 2015 Taylor & Francis.


PubMed | Jiangnan University and Huaian Entry Exit Inspection and Quarantine Bureau
Type: Journal Article | Journal: Sensors (Basel, Switzerland) | Year: 2015

A rapid, simple, sensitive, and specific immunochromatographic test strip was developed for the detection of Pantoea stewartii subsp. stewartii (Pss) in corn seed which was soaked overnight and then centrifuged for precipitate re-dissolved as samples. A pair of sensitive monoclonal antibodies for the immunochromatographic test strip was generated by mice immunization and cell fusion. Under optimized conditions, the lower detection limit of the strips for Pss was 1 10(5) cfu/mL both in 0.01 M phosphate buffer solution and corn seed samples, with no cross-reactivity with other common plant pathogens. The developed strip is useful and rapid for the detection of Pss in corn seed samples.


PubMed | Huaian Entry Exit Inspection and Quarantine Bureau and Jiangnan University
Type: | Journal: Biosensors & bioelectronics | Year: 2014

In this paper, we describe the development of a multicomponent lateral-flow assay based on an antibody-antigen reaction for the rapid and simultaneous detection of trace contaminants in water, including a heavy metal, algal toxin, antibiotic, hormone, and pesticide. The representative analytes chosen for the study were lead (Pb(II), microcystin-leucine-arginine (MC-LR), chloramphenicol (CAP), testosterone (T), and chlorothalonil (CTN). Five different antigens were immobilized separately in five test lines on a nitrocellulose membrane. The monoclonal antibodies specifically recognized the corresponding antigens, and there was no cross-reactivity between the antibodies in the detection assay. Samples or standards containing the five analytes were preincubated with the freeze-dried colloidal-gold-labeled monoclonal antibody conjugates to improve the sensitivity of the assay. The results were obtained within 20min with a paper-based sensor. The cut-off values for the strip test were 4ng/mL for Pb(II), 1ng/mL for MC-LR, 0.1ng/mL for CAP, 5ng/mL for T, and 5ng/mL for CTN. The assay was evaluated using spiked water samples, and the accuracy and reproducibility of the results were good. In summary, this lateral-flow device provides an effective and rapid method for the onsite detection of multiple contaminants in water samples, with no treatment or devices required.

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