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Huadong, China

Wang J.,Huadong Hospital | Zhang G.,Fudan University | Hu X.,Fudan University | Liu Y.,Fudan University | And 2 more authors.
Saudi Journal of Gastroenterology | Year: 2015

Background and Aim: To evaluate a high effective and practical regimen for the eradication of Helicobacter pylori infection. Patients and Methods: The 298 patients with H. pylori infection, diagnosed by biopsies performed during the endoscopy, were randomized into two groups. Group 1: Treated for one week with a combination of omeprazole, amoxicillin, and clarithromycin (OAC), named by OAC-1 group (n = 143); Group 2: OAC-2 group (n = 155) treated for two weeks with OAC. The OAC-1 group was treated with triple therapy of omeprazole 20 mg, amoxicillin 1000 mg, and clarithromycin 500 mg bid for 1 week. OAC-2 group was treated likewise, but for two weeks. A 13C-urea breath test was used to monitorH. pylori after four to eight weeks following therapy. Results: The eradication of infection was 55% and 68% in the OAC-1 and OAC-2 groups, respectively. Moreover, the eradication rates in the two groups were 63% and 75%, respectively. Compared with the OAC-1 group, the efficacy of treatment in the OAC-2 group is significantly higher (P < 0.05). Conclusion: Two-week OAC regimen yields a higher eradication rate of H. pylori, which might be a practical regimen for the eradication of H. pylori. © 2015 Saudi Journal of Gastroenterology (Official journal of The Saudi Gastroenterology Association).

Sun J.,Xinjiang Medical University | Guo X.,Huadong Hospital | Wugeti N.,Xinjiang Medical University | Guo Y.,Xinjiang Medical University | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

Background: Atrial fibrillation (AF) arises from abnormalities in atrial structure and electrical activity. Microelectrode arrays (MEA) is a real-time, nondestructive measurement of the resting and action potential signal, from myocardial cells, to the peripheral circuit of electrophysiological activity. This study examined the field action potential duration (fAPD) of the right atrial appendage (RAA) by MEA in rapid atrial pacing (RAP) in the right atrium of rabbits. In addition, this study also investigated the effect of potassium ion channel blockers on fAPD. Methods: 40 New Zealand white rabbits of either sex were randomly divided into 3 groups: 1) the control, 2) potassium ion channel blocker (TEA, 4-Ap and BaCl2), and 3) amiodarone groups. The hearts were quickly removed and right atrial appendage sectioned (slice thickness 500 μm). Each slice was perfused with Tyrode’s solution and continuously stimulated for 30 minutes. Sections from the control group were superfused with Tyrode’s solution for 10 minutes, while the blocker groups and amiodarone were both treated with their respective compounds for 10 minutes each. The fAPD of RAA and action field action potential morphology were measured using MEA. Results: In non-pace (control) groups, fAPD was 188.33 ± 18.29 ms after Tyrode’s solution superfusion, and 173.91 ± 6.83 ms after RAP. In pace/potassium ion channel groups, TEA and BaCl2 superfusion prolonged atrial field action potential (fAPD) (control vs blocker: 176.67 ± 8.66 ms vs 196.11 ± 10.76 ms, 182.22 ± 12.87 ms vs 191.11 ± 13.09 ms with TEA and BaCl2 superfusion, respectively, P < 0.05). 4-AP superfusion significantly prolonged FAPD. In pace/amiodarone groups, 4-Ap superfusion extended fAPD. Conclusions: MEA was a sensitive and stable reporter for the measurement of the tissue action potential in animal heart slices. After superfusing potassium ion channel blockers, fAPD was prolonged. These results suggest that Ito, IKur and IK1 remodel and mediate RAP-induced atrial electrical remodeling. Amiodarone alter potassium ion channel activity (Ito, IKur, IK1 and IKs), shortening fAPD. © 2015, E-Century Publishing Corporation. All Rights Reserved.

Ji J.,Shanghai Skin Diseases Hospital | Zhang L.-L.,Shanghai Skin Diseases Hospital | Ding H.-L.,Shanghai Skin Diseases Hospital | Wang H.-W.,Huadong Hospital | And 5 more authors.
Photodiagnosis and Photodynamic Therapy | Year: 2014

Objective: The aim of this pilot study was to compare the efficacy of ALA-PDT and red light alone in the treatment of photoaging. Methods: A total of 14 adults with photoaging skin were recruited. ALA-PDT or red light alone was applied to the forearm extensor. Before and after treatment, the treated sites were examined by dermoscopy, the changes in straum corneum (SC) hydration, transepidermal water loss (TEWL), and the L*. a*. b* values were measured, and microscopic examination of collagens and elastins was performed. Results: After ALA-PDT or red light illumination, the appearance of photoaging lesions improved, SC hydration increased and TEWL decreased. These changes in the ALA-PDT group were more obvious than those in the red light group. No significant change was noticed in the L*. a*. b* values in both groups. The signs of typical solar elastosis damage were improved in both groups. Conclusions: ALA-PDT showed better skin rejuvenation effect than red light alone. © 2014 Elsevier B.V.

Yao Y.,Huadong Hospital | Kong Z.,Fudan University
National Medical Journal of China | Year: 2015

Objective To evaluate the effect of Ixabepilone on quiescent or hypoxic cells response to ionizing radiation. Methods NCI-H460 and A549, two human NSCLC cell lines, were employed in this experiment. Quiescent cells (QC) or hypoxic cells (HC) were induced as mentioned previously and untreated cells as control. A colony forming assay was applied to compare cellular radio-sensitivity with or without Ixabepilone. Flow cytometry and Western blot analysis were used to detect cell cycle distribution and apoptosis. Results Along with an increased population of G1 cell (NCI-H46 P = 0. 001 3; A549 P = 0. 006) , both HC and QC were exhibited radio-resistance compared to untreated cells (survival fraction; NCI-H460 P = 0.003; A549 P = 0. 000 1). Moreover, it was found that Ixabepilone, which induced apoptosis in both IR-treated or untreated NSCLC cells, significantly enhanced cells death under hypoxia (decrease of survival fraction; NCI-H460 P = 0. 000 2; A549 P < 0. 01). Conclusion The existence of quiescent or hypoxic cells in solid tumors poses a critical therapeutic problem since they were resistant to IR. Ixabepilone, which induces apoptosis in NSCLC, showed great radio-sensitization effect on hypoxic cells. Following work will focus on whether Ixabepilone could increase hypoxic tumor cells radio-sensitivity in vivo, which could provide useful data for the application of Ixabepilone in clinical practice.

Yong D.,East China Normal University | Luo Y.,East China Normal University | Du F.,East China Normal University | Huang J.,East China Normal University | And 5 more authors.
Colloids and Surfaces B: Biointerfaces | Year: 2013

This research is aimed to develop a nano-sized supramolecular micelle delivery system of cis-dichlorodiammine platinum (II) (CDDP) in order to achieve the passive tumor targeting. Firstly, star-shaped poly (γ-benzyl-l-glutamate) was synthesized by the ring-opening polymerization of γ-benzyl-l-glutamate-N-carboxyanhydride initiated with per-6-amino-β-cyclodextrin. After removal of benzyl groups, β-cyclodextrin based seven-armed poly (l-glutamic acid) (β-CD-7PLGA) was obtained. β-CD-7PLGA/CDDP complexes were prepared by the complex reaction between the carboxylic groups of β-CD-7PLGA and CDDP. Further inclusion of β-CD-7PLGA/CDDP complexes with adamantine terminated mPEG (mPEG-Ad) gave CDDP supramolecular micelles (mPEG-Ad@β-CD-7PLGA/CDDP). The formation of mPEG-Ad@β-CD-7PLGA/CDDP supramolecular micelles was confirmed by fluorescence spectrophotoscopy and particle size measurements. All the micelles showed spherical shape, and their sizes increased from 100 to 135. nm with the increase of PLGA arm molecular weight. mPEG-Ad@CD-7PLGA/CDDP micelles showed sustained drug release profiles over 50. h in PBS. Compared with CDDP, mPEG-Ad@β-CD-7PLGA/CDDP supramolecular micelles showed essential decreased cytotoxicity to KB cells, suggesting their great potential as the delivery carriers of CDDP. © 2013 Elsevier B.V.

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