Bratislava, Slovakia
Bratislava, Slovakia
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Ondriska F.,HPL Ltd | Lengyel D.,Faculty Hospital | Miterpakova M.,Slovak Academy of Sciences | Lengyelova B.,Faculty Hospital | And 2 more authors.
Annals of Agricultural and Environmental Medicine | Year: 2010

The spread of dirofilariosis, as a newly emerging zoonosis, due to global changes has been documented in several Central European countries. In Slovakia, the first autochthonous case of dirofilariosis was recorded in dogs in 2005. The first case of human subcutaneous dirofilariosis caused by Dirofilaria repens was diagnosed in a 60-year-old patient from an area of western Slovakia. We report another case of dirofilariosis in a 37-year-old woman living in the same area of south-western Slovakia. The infection manifested as a painful, inflamed nodule on the back of the left hand, containing an adult parasite. Morphological analysis aroused suspicion of the presence of a filarial worm belonging to the genus Dirofilaria. Subsequent PCR analysis of the DNA identified the parasite as Dirofilaria repens. After removal of the parasite, the subcutaneous nodule completely resolved and the patient was successfully cured.

PubMed | Comenius University, HPL Ltd., Masaryk University and University Hospital Brno
Type: Journal Article | Journal: Medical mycology | Year: 2016

Despite advances in the treatment of invasive fungal diseases (IFD), mortality rates remain high. Moreover, due to the expanding spectrum of causative agents, fast and accurate pathogen identification is necessary. We designed a panfungal polymerase chain reaction (PCR), which targets the highly variable ITS2 region of rDNA genes and uses high resolution melting analysis (HRM) for subsequent species identification. The sensitivity and specificity of this method was tested on a broad spectrum of the most clinically important fungal pathogens including Aspergillus spp., Candida spp. and mucormycetes. Despite the fact that fluid from bronchoalveolar lavage (BAL) is one of the most frequently tested materials there is a lack of literature sources aimed at panfungal PCR as an IFD diagnostic tool from BAL samples. The applicability of this method in routine practice was evaluated on 104 BAL samples from immunocompromised patients. Due to high ITS region variability, we obtained divergent melting peaks for different fungal species. Thirteen out of 18 patients with proven or probable IFD were positive. Therefore, the sensitivity, specificity, positive predictive value and negative predictive value of our method were 67%, 100%, 100%, and 94%, respectively. In our assay, fungal pathogens identification is based on HRM, therefore omitting the expensive and time consuming sequencing step. With the high specificity, positive and negative predictive values, short time needed to obtain a result, and low price, the presented assay is intended to be used as a quick screening method for patients at risk of IFD.

Hrckova G.,Slovak Academy of Sciences | Kuchtova H.,Forlife N O General Hospital | Miterpakova M.,Slovak Academy of Sciences | Ondriska F.,HPL Ltd | And 2 more authors.
Journal of Helminthology | Year: 2013

Dirofilariosis is considered to be the arthropod vector-borne disease with the fastest spread in Europe. Slovakia belongs to new endemic regions for canine and human infections. This paper reports the fourth human case, where diagnosis was confirmed using computed tomography (CT), histological and molecular examinations - for the first time in this endemic region. The epidemiological history of this case indicated the autochthonous origin, and infection manifested by intense swelling in the periocular region, where a subcutaneous nodule was localized. Microscopic analysis of cross-sections of the surgically removed nodule confirmed the presence of a single male worm of Dirofilaria repens, indicated by the external cuticular ridges, the robust muscle cells and a single male sex organ seen as one tube beside the intestine. Considering that the worm morphology was partially damaged, molecular study was performed using DNA isolated from formalin-fixed and paraffin-embedded tissue sections of the worm. In polymerase chain reactions (PCR) a set of primers specific for D. repens DNA for the CO1-encoding gene amplified the expected 246 bp product using a high concentration of DNA template. Our diagnostic approach, involving molecular techniques, showed that identification of D. repens from excised tissues is possible even when the morphology of the worm and DNA are damaged during tissue processing. It is expected that the spread of this disease will continue due to climatic changes in central Europe. This warrants higher awareness among clinicians, who will initially be approached by patients, and better co-operation with parasitologists in newly endemic countries. Copyright © 2012 Cambridge University Press.

Mrva M.,Comenius University | Garajova M.,Comenius University | Lukac M.,Comenius University | Ondriska F.,HPL Ltd.
Journal of Parasitology | Year: 2011

Hexadecylphosphocholine (miltefosine) is an anticancer drug active in vitro against various protozoan parasites, and recently used for the treatment of disseminated Acanthamoeba infection. In the present study, we present results of weak cytotoxic activity of this potential amoebicidal agent for 2 of 3 clinical isolates of Acanthamoeba spp. Although the inhibition effect for all tested concentrations was apparent, and showed 100% eradication of trophozoites of Acanthamoeba castellanii strain at a concentration of 62.5 μM after 24 hr, the strains Acanthamoeba sp. and Acanthamoeba lugdunensis exhibited low sensitivity to hexadecylphosphocholine, even in high concentrations. The determined minimal trophocidal concentrations were 250 μM for Acanthamoeba sp. and 500 μM for A. lugdunensis after 24 hr of exposure. Although hexadecylphosphocholine is a potential agent for treatment of Acanthamoeba keratitis and systemic infections, in clinical practice the possible insusceptibility of the amoebic strain should be considered for optimizing therapy. © American Society of Parasitologists 2011.

Sykora T.,Haematopoietic Stem Cell Transplantation Unit | Horakova J.,Haematopoietic Stem Cell Transplantation Unit | Buzzasyova D.,Comenius University | Sladekova M.,HPL Ltd. | And 2 more authors.
New Microbes and New Infections | Year: 2014

The case presented here illustrates a protothecal infection caused by Prototheca wickerhamii in a paediatric haematopoietic stem cell recipient followed by a review of the literature of all 13 paediatric cases published since 1980. Protothecosis is a rare disease caused by algae, not described in this setting before. Infection was proven additionally post-mortem from peritoneal dialysis fluid. Even though no death of a paediatric patient due to this infection has been reported and the mortality rate associated with protothecosis is low, our patient died from multiorgan failure as a result of numerous post-transplant complications and a strain of cultivated alga that was highly resistant to antifungal agents. Prototheca spp. show various susceptibility profiles, and there is no direct correlation between in vitro activity and clinical response. There are different treatment regimens described but there are no clear published guidelines of specific therapy of protothecosis. Paediatric cases were successfully treated mostly with amphotericin B and azoles. As the number of immunocompromised patients increases, it is necessary to think more about unusual pathogens such as Prototheca. © 2014 The Authors.

Hpl Llc | Date: 2011-02-15

Desiccant for use as a drying agent and/or dehumidifier with cellular phones.

Hpl Llc | Date: 2011-09-20

dehumidifying substances, namely, desiccants for household and industrial use.

Hpl Llc | Date: 2010-05-15

Dehumidifiers; Dehumidifiers; Dehumidifiers for household use; Industrial dehumidifiers.

Hpl Llc | Date: 2011-08-23

Dehumidifiers; Dehumidifiers for household use.

Hpl Llc | Date: 2011-11-29

Dehumidifier kits consisting of a chemical desiccant within a resealable container for use in drying cellular telephones.

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