House Wellness Foods Corporation

Itami, Japan

House Wellness Foods Corporation

Itami, Japan
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Takahashi A.,Kyoto University | Yamamoto N.,House Wellness Foods Corporation | Murakami A.,Kyoto University
Life Sciences | Year: 2011

Aims: Overproduction of nitric oxide (NO) from inducible NO synthase (iNOS) plays many pivotal roles in various inflammatory diseases. In this study, we examined the effects of 6 flavonoids on lipopolysaccharide (LPS)-induced NO generation in macrophage (Mφ), and addressed molecular mechanisms of cardamonin. Main methods: Suppressive effects on NO generation in vitro were assayed in LPS-stimulated macrophages (Mφ). In vivo anti-inflammatory activity was evaluated with LPS-challenged ICR mice. Mechanistic analyses were done by ELISA, Western blot, RT-PCR, etc. Key findings: Cardamonin, a chalcone, exhibited pronounced suppressive activity, while pinocembrin, a counterpart flavanone, was much less active. Administration of cardamonin (0.02-2 mg/kg body weight) significantly decreased NOx concentrations in the sera from LPS-challenged ICR mice. This efficacy was superior to that of curcumin, a well-known anti-inflammatory agent present in turmeric. Cardamonin down-regulated iNOS mRNA expression and suppressed activation of STAT-1, but not nuclear factor kappaB, both of which are transcription factors for the iNOS gene in peritoneal Mφ of ICR mice. Interferon (IFN)-γ was identified as the major cytokine which mediates LPS-induced STAT-1 activation and resultant iNOS expression. Intriguingly, cardamonin suppressed the activation of not only STAT-1 but also STATs-2, 3 and 4. The involvement of the JAK/STATs pathway in NO generation was suggested because its specific inhibitor, AG490, decreased NO generation. Significance: Cardamonin was identified to be a unique phytochemical that targets the production of IFN- and thereby suppresses the STAT pathway for mitigating inflammation. © 2011 Elsevier Inc. All rights reserved.


Ohara T.,House Wellness Foods Corporation | Muroyama K.,House Wellness Foods Corporation | Yamamoto Y.,House Wellness Foods Corporation | Murosaki S.,House Wellness Foods Corporation
Phytotherapy Research | Year: 2015

To develop an anti-obesity agent, we examined the combination effect of glucosyl hesperidin (G-hesperidin) and caffeine on obesity in mice. High-fat diet-induced obese KK mice were fed a low-fat diet with or without G-hesperidin, caffeine, or their combination for 2 weeks. Decreases in body weight and significantly lower adipose tissue weight were observed in the combination-fed mice but not in the G-hesperidin-fed or caffeine-fed mice. DNA microarray analysis of mouse liver suggested that the feeding of G-hesperidin + caffeine was associated with lower lipogenesis. Therefore, we examined the anti-lipogenic effect of G-hesperidin + caffeine in fasted -refed KK mice. Hepatic triglyceride levels were significantly lower in the mice fed G-hesperidin + caffeine during the refeeding period but not in the mice fed each alone. In addition, hepatic expressions of genes related to lipogenesis, such as sterol regulatory element-binding protein-1c or fatty acid synthase, were significantly lower in the mice fed G-hesperidin + caffeine compared with that in the control mice. These results suggested that G-hesperidin + caffeine is effective for controlling obesity partly by the inhibition of hepatic lipogenesis. © 2014 John Wiley & Sons, Ltd.


Zhang T.,Kobe University | Sawada K.,Kobe University | Yamamoto N.,House Wellness Foods Corporation | Ashida H.,Kobe University
Molecular Nutrition and Food Research | Year: 2013

Scope: Adipocytes differentiation is deeply involved in the onset of obesity. 4-Hydroxyderricin (4HD) and xanthoangelol (XAG) are the chalcones that are derived from Ashitaba (Angelica keiskei). In this study, we demonstrated the inhibitory effects of these chalcones on adipocytes differentiation. Methods and results: 4HD and XAG suppressed intracellular lipid accumulation by Oil red O staining at 5 μM without cytotoxicity. They inhibited adipocytes differentiation accompanied by down-expression of adipocyte-specific transcription factors, CCAAT/enhancer-binding protein-β (C/EBP-β), C/EBP-α, and peroxisome proliferator-activated receptor gamma (PPAR-γ) using RT-PCR and Western blotting analysis. To obtain insights into the underlying mechanism, the activation of AMP-activated protein kinase (AMPK) and mitogen-activated protein kinase pathways was investigated. These two chalcones promoted phosphorylation of AMPK and acetyl CoA carboxylase during differentiation of 3T3-L1 adipocytes accompanied by a decrease in glycerol-3-phosphate acyl transferase-1 and an increase in carnitine palmitoyltransferase-1 mRNA expression. These chalcones also promoted phosphorylation of extracellular signal-regulated kinases and Jun aminoterminal kinases, but not p38. Moreover, the inhibitors for AMPK and extracellular signal-regulated kinases abolished the chalcones-caused down-expression of C/EBP-β, C/EBP-α, and PPAR-γ. Treatment with Jun aminoterminal kinases inhibitor abolished the down-expression of C/EBP-α and PPAR-γ, but not C/EBP-β. Conclusion: 4HD and XAG inhibit adipocytes differentiation through AMPK and mitogen-activated protein kinase pathways, resulting in the down-expression of adipocyte-specific transcription factors. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Patent
House Wellness Foods Corporation | Date: 2016-04-20

An object of the present invention is to provide a low-cost and safe approach to efficient delivery of a substance to phagocytes. In particular, the present invention relates to a carrier for delivery of a substance to phagocytes, the carrier comprising a lactic acid bacterium and/or an extract thereof.


Trademark
House Wellness Foods Corporation | Date: 2012-11-26

Tea; coffee; cocoa; confectionery; bread and buns; seasonings; spices; cereal preparations; instant confectionery mixes. Beer; extracts of hops for making beer; soft drinks; fruit beverages; whey beverages; vegetable juices.


Zhang T.,Kobe University | Yamamoto N.,House Wellness Foods Corporation | Ashida H.,Kobe University
Food and Function | Year: 2014

Excessive lipid accumulation in the liver has been proposed to cause hyperlipidemia, diabetes and fatty liver disease. 4-Hydroxyderricin (4HD), xanthoangelol (XAG), cardamonin (CAR) and flavokawain B (FKB) are chalcones that have exhibited various biological effects against obesity, inflammation, and diabetes; however, little is known about the inhibitory effects of these chalcones on fatty liver disease. In the present study, we investigated the ability of 4HD, XAG, CAR, and FKB to reduce lipid accumulation in hepatocytes. When HepG2 cells were treated with a mixture of fatty acids (FAs; palmitic acid:oleic acid = 1:2 ratio), significant lipid accumulation was observed. Under the same experimental conditions, addition of chalcones at 5 μM significantly suppressed the FA-induced lipid accumulation. We found that the expression of sterol regulatory element-binding protein-1 (SREBP-1), a key molecule involved in lipogenesis, was decreased in these chalcone-treated cells. We also found that these chalcones increased the expression of peroxisome proliferator-activated receptor α (PPARα), which is involved in FA oxidation. Moreover, these chalcones increased phosphorylation of AMP-activated protein kinase (AMPK) and liver kinase B1 (LKB1), upstream regulators of SREBP-1 and PPARα. We confirmed that an AMPK inhibitor, compound C, reversed chalcone-induced changes in SREBP-1 and PPARα expression in the HepG2 cells. Collectively, we found that 4HD, XAG, CAR, and XAG attenuated lipid accumulation through activation of the LKB1/AMPK signaling pathway in HepG2 cells. © 2014 the Partner Organisations.


Patent
House Wellness Foods Corporation | Date: 2010-04-21

Producing an immunostimulating composition containing lactic acid bacteria having IL-12 production-inducing activity, by culturing lactic acid bacteria belonging to the genus Lactobacillus, immediately killing the bacteria at the point of time at which the pH of the culture medium substantially stops decreasing, and then adding the obtained killed cells to a food-or-drink product.


Patent
House Wellness Foods Corporation | Date: 2013-06-11

An object of the present invention is to provide a low-cost and safe approach to efficient delivery of a substance to phagocytes. In particular, the present invention relates to a carrier for delivery of a substance to phagocytes, the carrier comprising a lactic acid bacterium and/or an extract thereof.


Patent
House Wellness Foods Corporation | Date: 2015-06-08

A composition comprising (a) and (b), and a food or drink or a medicine comprising the composition, wherein (a) is at least a kind of polyphenols selected from the group consisting of a polyphenol containing 15 mass % or more of proanthocyanidin having a polymerization degree of 1 to 3, hesperidin, a hesperidin derivative, and hesperetin; and (b) is at least a kind of xanthine derivatives, the mass ratio of (a):(b) in the composition being 1:(0.001 to 5), are useful for the prevention, amelioration, or treatment of metabolic syndrome, disorders of lipid metabolism (for example, fatty liver, hyperlipidemia), obesity (for example, visceral fat accumulation, subcutaneous fat accumulation), or the like.


Patent
House Wellness Foods Corporation | Date: 2011-01-14

A composition comprising (a) and (b), and a food or drink or a medicine comprising the composition, wherein

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