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Sanad M.H.,Hot Laboratories Center
Radiochemistry | Year: 2013

Omeprazole, a proton pump inhibitor, was labeled with 99m Tc to obtain 99m Tc-omeprazole in ∼96% yield in basic media. The optimum conditions were as follows: pH 9, 50 μg of SnCl2· 2H2O, 30 min, and 3 mg of the substrate. 99m Tc-omeprazole was stable for 6 h. Intravenous biodistribution of 99m Tc-omeprazole showed that it concentrated in the stomach ulcer to reach about 22% of the total injected dose at 1 h post injection. This concentration of 99m Tc-omeprazole in stomach ulcer may be sufficient for ulcer imaging. © 2013 Pleiades Publishing, Inc.

Amin A.M.,Hot Laboratories Center | Ibrahim I.T.,Hot Laboratories Center | Attallah K.M.,University of Umm Al - Qura | Ali S.M.,Hot Laboratories Center
Radiochemistry | Year: 2014

Sulfadimidine, a bacteriostatic drug blocking folic acid synthesis, was labeled with 99m Tc producing a yield of about 90%. The optimum conditions required to obtain 90% yield of the 99m Tc complex were as follows: 500 μg of sulfadimidine, 100 μg of SnCl2· 2H2O, 5 min, room temperature (25 ± 1 C), pH 4 (0.5 M citrate buffer). The radiochemical purity of the labeled compound was determined using paper and thin-layer chromatography. Biodistribution study in normal mice showed high uptake of the 99m Tc complex in stomach and intestine. The ratio of the uptake of the 99m Tc complex in muscles infected with E. coli to that in normal mice was about 2, 1.5 and 1.4 at 15, 90, and 180 min post injection, respectively, whereas for the muscles inflamed with heat-killed E. coli or sterile turpentine oil the difference from the normal muscles was insignificant. © 2014 Pleiades Publishing, Inc.

Tadros N.A.,Hot Laboratories Center | El-Sweify F.H.,Hot Laboratories Center
Journal of Radioanalytical and Nuclear Chemistry | Year: 2011

In the Egyptian society, it is usual until now that females wear extensively gold jewelry since their birth. The present work is carried out to determine gold content in scalp hair samples from some selected female donors of various ages. Instrumental Neutron Activation Analysis, with gamma ray spectrometry was applied for this study. The obtained data are compared with the available published data for analysis of hair samples from other countries. The correlation between age and gold concentration in hair samples is discussed. Interpretation of the influence of other factors which possibly may affect the contents of gold in the analyzed hair samples is also presented. © 2011 Akadémiai Kiadó, Budapest, Hungary.

Sanad M.H.,Hot Laboratories Center | El-Tawoosy M.,Hot Laboratories Center
Journal of Radioanalytical and Nuclear Chemistry | Year: 2013

An adopted method for the preparation of high radiochemical purity 99mTc-ursodeoxycholic acid (UDCA) was conducted with a high radiochemical yield up to 97.5 %. The reaction proceeds well using 2 mg UDCA, 50 μg tin chloride in solution of pH 8 at room temperature for 30 min. The radiochemical yield was up to 97.5 % as pure as 99mTc-UDCA. Different chromatographic techniques (paper chromatography and electrophoresis) were used to evaluate the radiochemical yield and purity of the labeled product. Biodistribution studies were carried out in Albino Swiss mice at different time intervals after administration of 99mTc-UDCA. The uptake of 99mTc-UDCA in the liver gave the chance to diagnose it. The results indicate that the labeled compound cleared from the systematic circulation within 2 h after administration and majority of organs showed significant decrease in uptake of 99mTc-UDCA. Finally, the liver uptake was high and the results indicate the possibility of using 99mTc-UDCA for hepatobiliary imaging. © 2013 Akadémiai Kiadó, Budapest, Hungary.

El-Tawoosy M.,Hot Laboratories Center
Radiochemistry | Year: 2013

A simple, fast, and efficient method was developed for preparing radioiodinated 125I-pindolol (125IPIN) using different oxidizing agents. 125I-PIN was prepared by the reaction between Na125I and pindolol using iodogen, Chloramine-T, N-bromosuccinamide, or H2O2 as an oxidizing agent at pH 7. The reaction proceeds well within 30 min at room temperature (20-25 C). The radiochemical yield was determined by TLC using chloroform-acetic acid-water (15: 4: 1) as developing solvent and by HPLC using reversed-phase RP18 column and acetonitrile-0.1 M ammonium bicarbonate (pH 7.5) (1: 1) as the mobile phase at a flow rate of 1 mL min-1. The radiochemical yield with the above oxidants was found to be 85, 93, 96.5, and 97.5%, respectively, with the radiochemical purity of 125I-PIN over 99.9%. © 2013 Pleiades Publishing, Inc.

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