Ruiz-Roso B.,Complutense University of Madrid |
Quintela J.C.,Puleva Biotech S.A. |
de la Fuente E.,Puleva Biotech S.A. |
Haya J.,Hospital Universitario Santa Cristina |
Perez-Olleros L.,Complutense University of Madrid
Plant Foods for Human Nutrition | Year: 2010
Recently, polyphenols have been found to affect blood lipids in animals in a similar manner as soluble dietary fibre. The aim was to assess whether an insoluble dietary fiber very rich in polyphenols has a beneficial effect on serum lipids in humans. In a double-blind randomized placebo-controlled clinical study with parallel arms, 88 volunteers with hypercholesterolemia were randomly assigned to consume daily either, fiber with insoluble 84% polyphenols 4 g twice a day (n=43) or placebo (n=45). Serum total, LDL and HDL cholesterol and triglycerides were assessed at baseline and after 4 weeks. The insoluble polyphenols consumption reduced the total cholesterol by 17.8 ± 6.1% (p<0.05), LDL cholesterol by 22.5 ± 8.9% (p <0.001), LDL: HDL cholesterol ratio by 26.2± 14.3% (p<0.001) and triglycerides by 16.3 ± 23.4% (p<0.05) at the end of the study compared with baseline. No significant differences were found during the study time in the placebo group for the lipid profile. The consumption of fiber very rich in insoluble polyphenols shows beneficial effects on human blood lipid profile and may be effective in prevention and treatment of hyperlipemia. © Springer Science+Business Media, LLC 2010.
Anedda A.,Hospital Universitario Of La Princesa |
Lopez-Bernardo E.,Hospital Universitario Of La Princesa |
Acosta-Iborra B.,Hospital Universitario Of La Princesa |
Acosta-Iborra B.,Hospital Universitario Santa Cristina |
And 5 more authors.
Free Radical Biology and Medicine | Year: 2013
Uncoupling protein 3 (UCP3) is a member of the mitochondrial inner membrane carrier superfamily that modulates energy efficiency by catalyzing proton conductance and thus decreasing the production of superoxide anion. However, its role during oxidative stress and the underlying regulatory and molecular mechanisms remain poorly understood. We sought to investigate how UCP3 expression is regulated by oxidative stress and to evaluate the putative antioxidant role of this protein. H2O2 treatment increased UCP3 expression and the nuclear accumulation of the transcription factor Nrf2 in C2C12 and HL-1 cells. Nrf2 siRNA prevented H2O2-induced UCP3 expression, increasing oxidative stress and cell death. ChIP assays identified an antioxidant-response element (ARE) within the UCP3 promoter that bound Nrf2 after exposure to H2O2. Luciferase reporter experiments confirmed increased ARE activity in H2O 2-treated HL-1 cells. Importantly, H2O2 increased the UCP3-mediated proton leak, suggesting a role for this protein in attenuating ROS-induced damage. Nrf2 nuclear accumulation and increased UCP3 protein were also detected in intact mouse heart subjected to a condition known to increase ROS generation. This is the first study to demonstrate that H 2O2 augments UCP3 expression and it provides the first evidence of Nrf2 binding to the UCP3 promoter in response to oxidative challenge. These findings suggest that UCP3 functions as a member of the cellular antioxidant defense system that protects against oxidative stress in vivo. In conclusion, we have identified a novel regulatory process induced by an oxidative insult whereby the expression of the mitochondrial protein UCP3 is driven by the Nrf2 transcription factor, which decreases ROS production and prevents cell death. © 2013 Elsevier Inc.
Fernandez-Fairen M.,Institute Cirugia Ortopedica Y Traumatologia Of Barcelona |
Hernandez-Vaquero D.,Hospital Of San Agustin |
Murcia A.,Hospital Of Cabuenes |
Torres A.,Hospital Universitario Santa Lucia |
Llopis R.,Hospital Universitario Santa Cristina
Clinical Orthopaedics and Related Research | Year: 2013
Background: Porous tantalum is an option of cementless fixation for TKA, but there is no randomized comparison with a cemented implant in a mid-term followup. Questions/purposes: We asked whether a tibial component fixed by a porous tantalum system might achieve (1) better clinical outcome as reflected by the Knee Society Score (KSS) and WOMAC Osteoarthritis Index, (2) fewer complications and reoperations, and (3) improved radiographic results with respect to aseptic loosening compared with a conventional cemented implant. Methods: We randomized 145 patients into two groups, either a porous tantalum cementless tibial component group (Group 1) or cemented conventional tibial component in posterior cruciate retaining TKA group (Group 2). Patients were evaluated preoperatively and 15 days, 6 months, and 5 years after surgery, using the KSS and the WOMAC index. Complications, reoperations, and radiographic failures were tallied. Results: At 5-year followup the KSS mean was 90.4 (range, 68-100; 95% CI, ± 1.6) for Group 1, and 86.5 (range, 56-99; 95% CI, ± 2.4) for Group 2. The effect size, at 95% CI for the difference between means, was 3.88 ± 2.87. The WOMAC mean was 15.1 (range, 0-51; 95% CI, ± 2.6) for the Group 1, and 19.1 (range, 4-61; 95% CI, ± 2.9) for Group 2. The effect size for WOMAC was -4.0 ± 3.9. There were no differences in the frequency of complications or in aseptic loosening between the two groups. Conclusions: Our data suggest there are small differences between the uncemented porous tantalum tibial component and the conventional cemented tibial component. It currently is undetermined whether the differences outweigh the cost of the implant and the results of their long-term performance. Level of Evidence: Level I, therapeutic study. See Instructions to Authors for a complete description of levels of evidence. © 2013 The Association of Bone and Joint Surgeons®.
Gonzalez-Rodriguez A.,Institute Investigaciones Biomedicas Alberto Sols CSIC UAM |
Gonzalez-Rodriguez A.,Research Center Biomedica En Red Of Diabetes fermedades Metabolicas Asociadas Ciberdem |
Gonzalez-Rodriguez A.,Hospital Universitario Santa Cristina |
Valverde A.M.,Institute Investigaciones Biomedicas Alberto Sols CSIC UAM |
Valverde A.M.,Research Center Biomedica En Red Of Diabetes fermedades Metabolicas Asociadas Ciberdem
Current Pharmaceutical Design | Year: 2015
RNA interference has emerged as an innovative technology for gene silencing that degrades mRNAs complementary to the antisense strands of double-stranded, short interfering RNAs (siRNAs). Its therapeutic application has important advantages over small-molecule drugs since offers the possibility of targeting virtually all genes and allows selective silencing of one or several genes. So far, a relative small proportion of cellular proteins can bind and respond to chemical drugs. Based on that, RNA interference-mediated gene silencing is widely considered as a crucial breakthrough in molecular biology with a direct translation to medicine. The liver has been widely chosen as a model system for the development of RNA interference therapy due to the convenience and availability of effective delivery into this tissue. Numerous preclinical models have revealed promising results, but the safety of this technology remains the primary challenge in developing siRNA based treatments. Liver diseases comprise a broad spectrum of genetic and non-genetic pathologies including acute fulminant liver injury that demands urgent medical care, or chronic pa- thologies such as nonalcoholic fatty liver (NAFLD), alcoholic liver disease, liver cirrhosis, viral hepatitis and hepatocellular carcinoma (HCC). In some cases restoration of liver function is not possible and alternatives to liver transplantation offering novel and efficient therapeutic approaches are urgently needed. In this review, we describe recent insights on the advantages of using RNA interference in preclinical settings as a targeted strategy with potential to markedly improve the treatment of liver diseases.
Vigano R.,Ferrari |
Marega L.,Casi di Cura S. Anna |
Breemans E.,Gelre Ziekenhuizen Apeldoorn |
Miro R.L.,Hospital Universitario Santa Cristina
Acta Orthopaedica Belgica | Year: 2012
Despite more than a decade of use, there are currently no comprehensive reviews summarising clinical results with the Profix Total Knee System in primary total knee arthroplasty. Searching the PubMed and Google Scholar databases revealed 17 potentially relevant Profix manuscripts. After author review and exclusion of studies not meeting predetermined variables, 8 manuscripts were selected. Knee Society data were provided in all 8 and implant survival data in 4. Data for 987 patients (1152 knees) were available. The overall estimated implant survival was 98.6% at 5 years and 94.2% at 10 years with revision for any reason as an endpoint, and 100% at both time points with radiographic loosening as an endpoint. Mean/median preoperative Knee Society knee scores improved from 39.2/24.7 at baseline, to 91.4/92.1 at the last postoperative follow-up visit. Good mediumto long-term clinical results can be expected with the Profix in primary total knee arthroplasty. © 2012, Acta Orthopædica Belgica.
Cabo J.,Hospital Universitario Santa Cristina |
Alonso R.,IIS Fundacion Jimenez Diaz |
Mata P.,IIS Fundacion Jimenez Diaz
British Journal of Nutrition | Year: 2012
Epidemiological and clinical studies suggest that consumption of omega (w-3) polyunsaturated fatty acids (PUFA) contributes to the reduction of cardiovascular mortality through different mechanisms including modulation of cellular metabolic functions, gene expression and beneficial effects on lipid profile or blood pressure. The aim of the study is to review the effects of w-3 PUFA supplemented as fish oil or blue fish in blood pressure. The analysis of different studies suggests that high doses w-3 PUFA ( ≥ 3 g/day) produces a small but significant decrease in blood pressure, especially systolic blood pressure, in older and hypertensive subjects; however, the evidence is not consistent among different studies. w-3 polyunsaturated fatty acids consumption might have a place in the control of patients with mild hypertension before starting drug treatment and of those who prefer changes of lifestyles like diet. © 2012 The Authors.
Biazzo A.,Hospital Universitario Santa Cristina |
Gonzalez del Pino J.,Hospital Universitario Santa Cristina
Revista Espanola de Cirugia Ortopedica y Traumatologia | Year: 2013
Introduction: The lipofibrohamartoma is a rare entity of unknown origin that can affect any peripheral nerves, but mainly being found in the median nerve within the carpal tunnel. The lipofibrohamartoma is frequently associated with other conditions such as macrodactyly, the Proteus and Klippel-Trenaunay-Weber syndromes and multiple exostosis, among others. Clinical cases: Two cases of lipofibrohamartoma in the carpal tunnel with associated median nerve palsy are described in the present article. They were treated by simple decompression of the median nerve by releasing the transverse carpal ligament and a palmaris longus tendon transfer to improve the thumb abduction (Camitz procedure). In one of the cases (a previously multi-operated median nerve entrapment at the carpal tunnel), a posterior interosseous skin flap was employed to improve the quality of the soft tissues on the anterior side of the wrist. Discussion: A review of the literature is also presented on lipofibrohamartoma of the median nerve, covering articles from 1964 to 2010. The literature suggests that the most recommended treatment to manage this condition is simple release of the carpal tunnel, which should be associated with a tendon transfer when a median nerve palsy is noticed. © 2013 SECOT.
Porrero J.L.,Hospital Universitario Santa Cristina |
Cano-Valderrama O.,Hospital Universitario Santa Cristina |
Castillo M.J.,Hospital Universitario Santa Cristina |
Alonso M.T.,Hospital Universitario Santa Cristina
Hernia | Year: 2015
Purpose: Self-gripping meshes have been developed to avoid fixing sutures during inguinal hernia repair. Operative time is shorter when using a self-gripping mesh than with conventional Lichtenstein repair. However, these meshes can be difficult to handle because they fix to undesired structures. The aim of this report is to describe a new technique to avoid this problem. Technique: Inguinal hernia dissection is made as usual. Once dissection is finished, a Parietex ProGrip® (Covidien, Dublin, Ireland) flat sheet mesh is cut depending on the size needed. A small split is made between the lower and medium third of the mesh to mark where the split for the spermatic cord will be. Using this mark, the upper third of the mesh is folded over the medium third, hiding the microgrips that make this a self-gripping mesh. In this way, only the lower third of the mesh has the microgrips exposed and the mesh can be fixed to the pubic bone and inguinal ligament without fixation to undesired structures. Once the lower third of the mesh is fixed, the split for the spermatic cord is completed and the upper part of the mesh is passed below the spermatic cord. Then, the mesh is unfolded to expose the microgrips again and the medium and upper third of the mesh are descended to its final position. Conclusion: This proposed technique for inguinal hernia repair with self-gripping mesh makes the surgery easier, avoiding mesh fixation to undesired structures. © 2014, Springer-Verlag France.
Alpha blockers: Effects on improvement of erectile dysfunction and sexual desire in patients with benign prostatic hypertrophy [Alfabloqueantes: Efectos en la mejora de la disfunción eréctil y el deseo sexual en pacientes con hipertrofia prostática benigna]
Balmori C.,Hospital Universitario Santa Cristina
Revista Internacional de Andrologia | Year: 2013
Objectives: To assess the impact of benign prostatic hyperplasia on both quality of life and sexual function as well as the benefits of using alpha-blockers, especially silodosin. Material and method: Epidemiological observational, multicenter nationwide study in which the data collection was performed retrospectively. A total of 175 urologists, who recruited 900 patients, participated in the study. Descriptive statistics of all variables were carried out, including measures of central tendency, dispersion for quantitative variables, and absolute and relative frequencies for qualitative variables. Results: At the beginning of treatment, 31.6% of the patients reported a lack of desire. This proportion decreased to 26.6% at the end of the study (P< .0001). When the treatment was initiated, 64.6% of patients had mild or absent erectile dysfunction. This has increased to 71% at the present time (P= .0002). Conclusions: Adequate selection of patients who may benefit from treatment with alpha-blocker, explanation of their benefits and side effects will not only improved lower urinary tract symptoms, but also erectile dysfunction scores. In this case, the most uroselective alpha-blocker silodosin has shown excellent results in both fields, both when administered as monotherapy or associated with PDE5 inhibitors. Furthermore, use of silodosin improved sexual desire parameter as opposed to use of 5 alpha reductase inhibitors. © 2013 Asociación Española de Andrología, Medicina Sexual y Reproductiva.
Miquilena Colina M.E.,Hospital Universitario Santa Cristina |
Garcia Monzon C.,Hospital Universitario Santa Cristina
Gastroenterologia y Hepatologia | Year: 2010
Obesity is associated with a higher risk of developing non-alcoholic fatty liver disease (NAFLD) and contributes to the progression of liver diseases of distinct etiologies such as chronic hepatitis C virus (HCV) infection. The discovery that adipose tissue is submitted to a state of chronic inflammation able to secrete adipokines has allowed a connection to be established between the metabolic alterations that lead to triglyceride accumulation and liver inflammation, reinforcing the role of hepatocellular lipotoxicity in the pathogenesis of NAFLD. In addition, although HCV genotype 3 induces steatosis, it is currently believed that obesity and its associated alterations, such as insulin resistance, are involved in progression of HCV-mediated liver disease, as well as that of other chronic liver diseases of diverse etiologies. © 2009 Elsevier España, S.L.