de Lemos P.A.P.,Hospital of Tropical Diseases |
Garcia-Zapata M.T.A.,Hospital of Tropical Diseases
International Journal of Tropical Medicine | Year: 2010
The frequency of Trichomonas vaginalis infection in HIV-positive and negative women attending hospitals in Goiania, Brazil was evaluated using the gold standard diagnostic method of culture. A total of 237 vaginal swab specimens were examined: 125 (52.7%) comprising the HIV-positive group and 112 (47.3%) the HIV-negative control group. T. vaginalis was detected in 13.5% of the women, 23 (18.4%) of whom were HIV-positive while 9 (8.0%) were HIV-negative. This difference was statistically significant however, infection by this parasite was not found to be associated with immune status. T. vaginalis was found in 23.1% of the pregnant women and there was a statistically significant difference in the rate of infection by this parasite between the pregnant HIV-positive and the pregnant HIV-negative women (25.8% versus 12.5%). T. vaginalis was more prevalent in HIV-positive compared to HIV-negative women however, no association was found between the infection and the immune status of the patients. © Medwell Journals, 2010.
Tho D.Q.,University of Oxford |
Lan N.T.N.,Pham Ngoc Thach Hospital for Tuberculosis and Lung Diseases |
Chau N.V.V.,Hospital of Tropical Diseases |
Farrar J.,University of Oxford |
Caws M.,University of Oxford
International Journal of Tuberculosis and Lung Disease | Year: 2011
SETTING: Pham Ngoc Thach Tuberculosis Reference Hospital, Ho Chi Minh City, Viet Nam. DESIGN: A multiplex allele-specific polymerase chain reaction (MAS-PCR) was developed to detect mutations at the two most common sites responsible for isonia-zid (INH) resistance in Mycobacterium tuberculosis: katG315 and inhA-15. The MAS-PCR is able to detect rare mutations at katG315, in addition to katG S315T. Conventional phenotypic proportion drug susceptibility testing on Löwenstein-Jensen media was used as a gold standard to compare the sensitivity and specificity of the commercial MTBDRplus line-probe assay and the MAS-PCR in 100 INH-resistant and 50 INH-susceptible isolates collected consecutively at Pham Ngoc Thach Hospital reference laboratory. RESULTS: The sensitivity and specificity on culture isolates were 90% (n = 90/100, 95%CI 0.83-0.94) and 100% (n = 50/50, 95%CI 0.93-1.0), respectively, for the MAS-PCR and the MTBDRplus assay. CONCLUSION: The MAS-PCR described here represents an alternative method for rapid screening for INH resistance in M. tuberculosis isolates. © 2011 The Union.
Nguyen H.P.,Hospital of Tropical Diseases |
Hanson J.,Cairns Base Hospital |
Bethell D.,A+ Network |
Nguyen T.H.,Hospital of Tropical Diseases |
And 11 more authors.
PLoS ONE | Year: 2011
Background: Optimising the fluid resuscitation of patients with severe malaria is a simple and potentially cost-effective intervention. Current WHO guidelines recommend central venous pressure (CVP) guided, crystalloid based, resuscitation in adults. Methods: Prospectively collected haemodynamic data from intervention trials in Vietnamese adults with severe malaria were analysed retrospectively to assess the responses to fluid resuscitation. Results: 43 patients were studied of whom 24 received a fluid load. The fluid load resulted in an increase in cardiac index (mean increase: 0.75 L/min/m2 (95% Confidence interval (CI): 0.41 to 1.1)), but no significant change in acid-base status post resuscitation (mean increase base deficit 0.6 mmol/L (95% CI: -0.1 to 1.3). The CVP and PAoP (pulmonary artery occlusion pressure) were highly inter-correlated (rs = 0.7, p&0.0001), but neither were correlated with acid-base status (arterial pH, serum bicarbonate, base deficit) or respiratory status (PaO2/FiO2 ratio). There was no correlation between the oxygen delivery (DO2) and base deficit at the 63 time-points where they were assessed simultaneously (rs=-0.09, p=0.46). Conclusions: In adults with severe falciparum malaria there was no observed improvement in patient outcomes or acid-base status with fluid loading. Neither CVP nor PAoP correlated with markers of end-organ perfusion or respiratory status, suggesting these measures are poor predictors of their fluid resuscitation needs. © 2011 Phu et al.