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Zhu F.,Hospital of Traditional Chinese Medicine | Ke F.,Shiyan Peoples Hospital
International Eye Science | Year: 2017

AIM: To investigate the role of regulatory T cells in the pathogenesis of autoimmune dry eye and to analyze the expression of endogenous regulatory T cells and related cytokines in the lacrimal system to provide a more effective treatment for autoimmune dry eye disease treatment programs. METHODS: Rabbit lacrimal gland epithelial cells isolated and cultured for a period of time were separately cultured and mixed with isolated peripheral blood lymphocytes in a ratio of 1:1 and cultured with 5-Bromo-2-deoxyUridine (BrdU) to detect the proliferation of peripheral blood lymphocytes, and the activated autologous peripheral blood lymphocytes were transferred to donor rabbits via ear vein. The rabbits were used as the rabbit model of autoimmune dry eye and the normal rabbits which did not induce the disease as the control group. Before and after the experiment, tear film rupture time and tear secretion at 2, 4, 6, 8 wk, were observed. At 4 wk after the transfusion the rabbits were sacrificed for the collection of bilateral upper and lower lacrimal gland and conjunctiva, and pathological HE staining, and then extract the total RNA in lacrimal gland. The expression of IL-17, TNF-α, IL-6 and TGF-γ was detected. RESULTS: BrdU detected peripheral lymphocyte proliferation rate of 3.72. Compared with the normal group, the time of rupture of the tear film decreased (P<0.05), and the tear of the model group was significantly lower than that of the control group (P<0.05). The expression of IL-17, TNF-α, TGF-γ and IL-6 in the model group were significantly higher than those in the control group (P<0.05). The expression of IL-10 and TGF-β was significantly lower than those of the control group (P<0.05). The ratio of CD4+ CD2+ Treg cells in lacrimal gland tissue and spleen was higher in control group. The proportion of CD4+CD25+ Treg cells in model group decreased (P<0.05). CONCLUSION: IL-17, TNF-α, IL-6 and TGF-γ play important roles in inflammatory reaction of lacrimal gland and the immunodepression active by CD4+CD25+Treg cells decreases when the rabbit model of dry eye is involved. Copyright 2017 by the IJO Press.

Cai X.B.,Shanghai JiaoTong University | Dai Y.M.,Hospital of Traditional Chinese Medicine | Wan X.J.,Shanghai JiaoTong University | Zeng Y.,Shanghai JiaoTong University | And 3 more authors.
Digestive Diseases and Sciences | Year: 2013

Background: Intrasphincteric injection of botulinum toxin (BTX) and removable self-expanding metal stents (SEMS) have both been used for the treatment of achalasia. Aim: To compare the effectiveness and long-term outcome of SEMS placement and BTX injection in patients with achalasia. Methods: A total of 110 patients with newly diagnosed achalasia were enrolled in the study between July 2003 and December 2009. The patients received either BTX injection (n = 51) or SEMS placement (n = 59). Clinical symptoms, objective parameters, and long-term clinical outcomes were evaluated after 7 and 30 days and then every 6 months until withdrawal from the study. The mean duration of follow-up was 28 months (range 10-36 months). Results: Improvements in global symptom and dysphagia scores and in basal lower esophageal sphincter pressure were significantly more marked in the SEMS group than in the BTX group (all P < 0.05). After 12 months, the remission rate in the SEMS group (81.28 %) was statistically significantly higher than that in the BTX group (64.58; P < 0.05). At 36 months, the remission rates in the two groups were 49.1 and. 4.2 %, respectively (P < 0.01). The symptom-free interval was 831 ± 46 days in the SEMS group and 522 ± 42 days in the BTX group (P < 0.01). No side effects were reported with BTX treatment. In the SEMS group there were 13 episodes of chest pain, nine cases of regurgitation, and four stent migrations. No serious complications occurred. Conclusion: Removable SEMS was associated with better long-term outcomes than BTX injection in patients with achalasia. © 2013 Springer Science+Business Media New York.

Teng J.,Hospital of Traditional Chinese Medicine | Wang X.,Fujian Medical University | Xu Z.,Hospital of Traditional Chinese Medicine | Tang N.,Fujian Medical University
Journal of Cellular Biochemistry | Year: 2013

This study investigated the molecular mechanisms of liver cells with HBx expression on epithelium-mesenchymal transition (EMT) change using Western blot analysis and Transwell assay to assess EMT-related protein expression and cell mobility. Luciferase reporter assay and chromatin immunoprecipitation (ChIP) assay were used to test the Twist promoter containing different STAT3 binding loci. Electrophoretic mobility band-shift assay (EMSA) was used to detect Twist activity. Results showed that HBx expression affected the EMT-related protein expression and the cell mobility of liver cancer cells (MHCC97) and liver cells (HL-7702) in vitro or in vivo. These proteins exhibited reversed expression to a certain extent after Twist inhibition. In addition, the wound-healing capability and the mobility of HL-7702/HBx cells were lower than those treated with control-siRNA. The expressions of p-STAT3 and Twist were positively correlated with HBx expression. The second STAT-3 binding sequence in the Twist promoter region of the HL-7702/HBx cells was the first locus. Twist activity in the HL-7702/HBx2 cells was higher than that in HL-7702 cells. Moreover, the activity decreased when the cells were treated with HBx-siRNA to inhibit HBx expression, or with STAT3 inhibitor to reduce STAT3 activation. Therefore, Twist is essential for the regulation of the mobility of liver cells with HBx expression. HBx activates the Twist promoter by activating STAT3 and promotes EMT occurrence in liver cells. Copyright © 2012 Wiley Periodicals, Inc.

Wang Y.,Hospital of Traditional Chinese Medicine
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2013

To study effects of Xinfeng Capsule (XFC, a Chinese herbal compound for benefiting qi, invigorating Pi, and dredging col laterals) on myocardial fibrosis in adjuvant arthritis (AA) rats and its mechanism study. Sixty male Wistar rats were randomly divided into the normal control (NC) group (n = 12) and the model control (MC) group (n =48). Except rats in the NC group, Freund's complete adjuvant (0. 1 mL) was intracutaneously injected from the right hind limb to prepare the AA rat model. On the 19th day of inflammation rats in the AA model group were randomly divided into four groups, i.e., the XFC group, the methotrexate (MTX) group, the Tripterygium wilfordii polycoride Tablet (TPT) group, and the MC group, 12 in each group. Rats were sacrificed after 30-day medication. The myocardial ultrastructure was observed under transmission electron microscope. The mRNA and protein expression levels of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) were detected using RT-PCR and Western blot respectively. (1) Results under electron microscope showed obvious hyperplasia of collagen fibers in myocardial cells of AA rats, which indicated the trend of myocardial fibrosis. The myocardial ultrastructural changes could be effectively improved in the MTX group, the TPT group, and the XFC group. The morphology of myocardial cells in the XFC group was contact, with clear nucleus, and little collagen fibers, which was better than the other groups. (2) Compared with the NC group, the mRNA and protein expression levels of MMP-9 in the myocardial tissue of the MC group were obviously up-regulated, while the mRNA and protein expression levels of TIMP-1 were obviously down-regulated (P <0. 01). The mRNA and protein expression levels of MMP-9 decreased, and the mRNA and protein expression levels of TIMP-1 obviously increased in the XFC group, showing statistical difference when compared with the MC group (P < 0. 5, P < 0. 01). XFC could effectively inhibit the myocardial fibrosis of AA rats.lts mechanisms might be correlated with inhibiting the mRNA and protein expression levels of MMP-9, improving the mRNA and protein expression levels of TIMP-1, adjusting the balance of MMP-9/TIMP-1 ,and improving the metabolism of myocardial extracellular matrix.

Zhou Y.Y.,Hospital of Traditional Chinese Medicine | Zhang R.-J.,Hospital of Traditional Chinese Medicine
International Journal of Ophthalmology | Year: 2010

AIM: To observe clinical effects of Avastin combined with vitreous cavity injection of triamcinolone acetonide in treatment of diabetic retinopathy ( DR) with macular edema (ME). METHODS: Thirty-four cases of the DR with ME inpatients or outpatients in our hospital from March 2007 to April 2008, with both eyes involved in each case, were randomly divided into observation group of 17 cases and control group of 17 cases. Observation group were treated with intravitreal Avastin (20g/L), triamcinolone acetonide (40g/L) co-injection and the control group received intravitreal triamcinolone acetonide (40g/L) injection. The visual acuity, intraocular pressure, retinal neovascularization and the ME situation were observed and recorded. RESULTS:The cure rate of the observation group was 64.7%, while the cure rate of the control group was only 41.2% . Observation group was significantly better than control group in the cure rate (P<:0.05). CONCLUSION: Avastin combined with vitreous cavity injection of triamcinolone acetonide in treatment of DR with ME is an effective approach.

Zhang Y.-M.,Hospital of Traditional Chinese Medicine | Huang Q.-F.,Meridian
Journal of Acupuncture and Tuina Science | Year: 2014

In review of acupuncture literature from 1949 to 2010, in accordance with the data in 'Traditional Chinese Medical Acupuncture-moxibustion Information Databank', the accounting analysis was used for comprehensive analysis and regularity summery of acupuncture therapy for apoplexy sequelae. The results showed that Hegu (LI 4), Zusanli (ST 36), Quchi (LI 11), Jianyu (LI 15), Sanyinjiao (SP 6), Yanglingquan (GB 34), Lianquan (CV 23), and Waiguan (TE 5) were used at the highest frequency. In the therapeutic methods, acupuncture was used at the highest frequency, followed by scalp acupuncture, and simultaneous application of acupuncture and herbal medicine. © 2014 Shanghai Research Institute of Acupuncture and Meridian and Springer-Verlag Berlin Heidelberg.

Shao H.,Hospital of Traditional Chinese Medicine | Ma J.,Hospital of Traditional Chinese Medicine | Guo T.,Hospital of Traditional Chinese Medicine | Hu R.,Hospital of Traditional Chinese Medicine
Experimental and Therapeutic Medicine | Year: 2014

Triptolide is a diterpene triepoxide compound extracted from the medicinal plant, Tripterygium wilfordii Hook F. The aim of the present study was to determine whether triptolide inhibits the proliferation of breast cancer cells and to further investigate the associated molecular mechanisms. The effects of triptolide on the cell viability of three breast cancer cell lines, specifically, highly metastatic MDA-MB-231, human epidermal growth factor receptor 2-positive BT-474 and estrogen receptor-positive MCF7 cells, were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and apoptosis assays. Western blot analysis was performed to investigate the expression levels of β-catenin in the control and triptolide-treated cells. The results demonstrated that triptolide treatment caused cell death in the three types of malignant cell lines. Treatment with 25 nM triptolide for 48 h exhibited marked inhibitory effects on the cell viability of the three types of cells, with greater effects observed in BT-474 cells compared with the other two cell types. When compared with the cells not treated with triptolide, 50 nM triptolide treatment resulted in apoptosis of MDA-MB-231, BT-474 and MCF7 cells with apoptotic rates of ~80%. Western blot analysis indicated that triptolide treatment of MDA-MB-231, BT-474 and MCF7 cells decreased the expression levels of β-catenin to 5-10% of the levels observed in the cells treated with dimethyl sulfoxide only. Therefore, the results of the present study indicate that triptolide induces the apoptosis of breast cancer cells via a mechanism associated with the Wnt/β-catenin signaling pathway.

Shao H.,Hospital of Traditional Chinese Medicine | Yang B.,Hospital of Traditional Chinese Medicine | Hu R.,Hospital of Traditional Chinese Medicine | Wang Y.,Hospital of Traditional Chinese Medicine
Oncology Letters | Year: 2013

Matrine is an alkaloid isolated from Sophora flavescens. The present study aimed to determine whether matrine effectively inhibits the proliferation of breast cancer cells, and the underlying mechanism(s) of its antitumor function. The effects of matrine on the cell viability of ER-positive MCF7 cells, HER2-positive BT-474 cells and highly metastatic MDA-MB-231 cells were measured using MTT and apoptosis assays. Western blot analysis was performed to investigate the expression levels of the inhibitor of κB (IκB) kinase β (IKKβ) in cells treated with or without matrine. It was observed that the matrine treatment resulted in the death of the three types of cancer cells, but significantly less toxicity was observed in the control cancer cells. The experimental results also suggested that the antitumor effects of matrine on breast cancer cells may be associated with the downregulation of IKKβ expression by matrine, as indicated by the western blot analysis results. The present results suggested that matrine may be used as an effective drug candidate for treating breast cancers in the future, following further research.

Zhang Y.-F.,Hospital of Traditional Chinese Medicine | Dong X.-S.,Hospital of Traditional Chinese Medicine
Cancer Research and Clinic | Year: 2013

Objective: To investigate the effect of ursodesoxycholic acid (UDCA) on JAK/STAT3/ COX-2 signal pathway in human hepatocellular carcinoma cells and the underlying mechanism of its antitumor effect. Methods: In order to obtain the optimal concentration and time of UDCA, MTT assay was used to detect anti-proliferative activities of UDCA on SMMC-7721 cells induced by IL-6 in vitro. Cells were divided into control group, IL-6 group, AG490 (JAK2 inhibitor) group and UDCA group. The protein expressions of STAT3, p-STAT3 and COX-2 were measured by Western blot. Results: MTT assay showed UDCA had obvious anti-proliferative activities on SMMC-7721 cells. The optimized concentrations were 50, 100, 200 μg/ml, the optimized culture time was 48 h. Western blot results showed that the expression of p-STAT3 and COX-2 were remarkably increased in IL-6 group than that in control group. There was no difference of STAT3 expression between the two groups. Compared with IL-6 group, the protein level of COX-2 was decreased in AG490 and UDCA groups, so was the expression of p-STAT3. But there was no change on STAT3 expression in AG490 and UDCA groups. Conclusion: UDCA may decrease the proliferation of SMMC-7721 cells via inhibiting phosphorylation of STAT3 and down-regulating COX-2 expression.

Zhang L.J.,Hospital of Traditional Chinese Medicine
Zhongguo gu shang = China journal of orthopaedics and traumatology | Year: 2012

To study the application of stress X-ray of knee joint for the diagnosis ot medial collateral ligament injury. From January 2008 to June 2011, 46 patients with medial collateral ligament injury were reviewed. Among the patients, 32 patients were male and 14 patients were female, ranging in age from 28 to 72 years, with an average of (49.46 +/- 22.54) years. Left knee joint injuries occurred in 22 patients, and 24 patients had injuries in right knee joint. Thirty-one patients were treated with surgical method, and 15 patients were treated with conservative method including 1 patient finally received operation after 3 months treatment with conservative method without effect. The injury side and normal side were observed by X-ray under stress and non-stress, as well as MRI. Operation was used to treat patients with medial gap markedly widen and the deep layer of medial collateral ligament injury in MRI. A line (line A) was drawn from medial condyle to lateral condyle of tibia platform. The line A was translated to the inner margin of condylus medialis femoris, which was line B. The perpendicular distance was measured, which was line C. The ration of space difference (R) was calculated by (space at stress force of injured side-space at non-stress force of injured side)/(space at stress force of normal side-space at non-stress force of normal side). The relationship between R and ligament injury type were investigated. There were 17 patients with injuries of superficial lamella, 21 patients with injuries of deep lamella. Eight patients had associated injuries of articular capsule and posterior cruciate ligament. When R value was between 1.51 to 5.24, the injury of superficial ligament was found in 15 patients, actual injuries were found in 17 patients. When R value between 5.28 and 13.85, the injuries of bathypelagic ligament were found in 19 patients, actual injuries were found in 21 patients. When R value was between 15.61 and 26.25, the associated injuries of articular capsule and posterior cruciate ligament were found in 7 patients, actual injuries were found in 8 patients. Using the specific value as the standard of measurement can provide the quantizatily fractionation of the medial collateral ligament injury.

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