Hospital of Kunming Medical College

Kunming, China

Hospital of Kunming Medical College

Kunming, China
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Wang Y.,Hospital of Kunming Medical College | Liu L.,Hospital of Kunming Medical College | Tang H.,Hospital of Kunming Medical College | Zhang L.,Hospital of Kunming Medical College | He Q.,Hospital of Kunming Medical College
Journal of Leukemia and Lymphoma | Year: 2011

Objective To explore the p27 gene expression and clinical significance in acute leukemia (AL). Methods RT-PCR was used for testing the mRNA levels of p27 gene in bone marrow mononuclear cell of 65 cases of newly diagnosed AL patients and 41 controls. The correlation between the p27 gene expression positive rate and age, sex, peripheral blood white blood cell count and extramedullary infiltration were analyzed simultaneously in AL patients. Results The positive rate of p27 gene expression (36.9%, 24/65) was significantly lower in AL group than that in the control group (87.8%, 36/41) (P <0.05), and which was lower than the control group (P <0.05) in the AML group (34.2%, 13/38) and the ALL group (37.5%, 9/24). However, comparing AML group with ALL group, the difference was not statistically significant (P >0.05). At the same time, compared the p27 gene expression positive rate of > 14 years old group (52 cases) and <14 years old group (13 cases), and also compared in male group (34 cases) and in female group (31 cases), there was no statistically difference (P >0.05); Compared the p27 gene expression positive rate of peripheral blood white blood cell count >20×l09/L group (31 cases) and <20×l09/L group (34 cases), also compared with in extramedullary infiltration group (37 cases) and in no extramedullary infiltration group (28 cases), the relationship of p27 gene expression positive rate with peripheral blood white blood cell count as well as extramedullary infiltration was a negative correlation (r = -0.284, P < 0.05, r = -0.300, P < 0.05). Conclusion There is p27 mRNA expression deletion in AL patients, and the deletion is present in the AML and ALL at the same time. p27 mRNA expression is negatively correlated with peripheral blood white blood cell count or extramedullary infiltration, which might be one of the mechanisms of occurrence and development of AL.


Ding X.,Wuhan University | Yan D.,Hospital of Kunming Medical College | Long Q.,Wuhan University
Medical Journal of Wuhan University | Year: 2010

Objective: To investigate the effect of using the suspension of ferroso-ferric oxide granules and iodinated oil to embolize the artery of primary hepatic carcinoma. Methods, Ninety-eight cases with primary hepatic carcinoma who had accepted TACE were retrospectively studied and were divided into three groups according to the method of treatment as group A (suspension of iodinated oil embolism and chemotherapeutics), group B (suspension of iodinated oil embolism, chemotherapeutics, and the stem artery embolizing by spongia gelatinosa) , and group C (suspension of ferroso-ferric oxide granules iodinated oil and chemotherapeutics). Results, The nulli-recanalization rate of group B and group C were obviously higher than that of group A (both P< 0. 01), however, the part-recanalization rate and the all-recanalization rate of group A and B were lower (all P<0. 01). There was no statistical differences in recanalization between group B and group C (P>0. 05). Conclusion, After embolization, the revascularization results were different according to the embolizing method adopted. The method of using the suspension of ferroso-ferric oxide granules and iodinated oil is the more effective one.


Wu X.,Yunnan Normal University | Cheng J.,Kunming University | Yang K.,Hospital of Kunming Medical College
International Journal of Molecular Sciences | Year: 2016

Epidemiological studies regarding the relationship between vitamin D, genetic polymorphisms in the vitamin D metabolism, cigarette smoke and non-small cell lung cancer (NSCLC) risk have not been investigated comprehensively. To search for additional evidence, the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique and radioimmunoassay method were utilized to evaluate 5 single-nucleotide polymorphisms (SNPs) in vitamin D receptor (VDR), 6 SNPs in 24-hydroxylase (CYP24A1), 2 SNPs in 1α-hydroxylase (CYP27B1) and 2 SNPs in vitamin D-binding protein (group-specific component, GC) and plasma vitamin D levels in 426 NSCLC cases and 445 controls from China. Exposure to cigarette smoke was ascertained through questionnaire information. Multivariable linear regressions and mixed effects models were used in statistical analysis. The results showed that Reference SNP rs6068816 in CYP24A1, rs1544410 and rs731236 in VDR and rs7041 in GC were statistically significant in relation to reduction in NSCLC risk (p < 0.001-0.05). No significant connection was seen between NSCLC risk and overall plasma 25-hydroxyvitamin D [25(OH)D] concentrations, regardless of smoking status. However, the mutation genotype of CYP24A1 rs6068816 and VDR rs1544410 were also significantly associated with increased 25(OH)D levels only in both the smoker and non-smoker cases (p < 0.01-0.05). Meanwhile, smokers and non-smokers with mutated homozygous rs2181874 in CYP24A1 had significantly increased NSCLC risk (odds ratio (OR) = 2.14, 95% confidence interval (CI) 1.47-3.43; p = 0.031; OR = 3.57, 95% CI 2.66-4.74; p = 0.019, respectively). Smokers with mutated homozygous rs10735810 in VDR had significantly increased NSCLC risk (OR = 1.93, 95% CI 1.41-2.76; p = 0.015). However, smokers with mutated homozygous rs6068816 in CYP24A1 had significantly decreased NSCLC risk (OR = 0.43, 95% CI 0.27-1.02; p = 0.006); and smokers and non-smokers with mutated homozygous rs1544410 in VDR had significantly decreased NSCLC risk (OR = 0.51, 95% CI 0.34-1.17; p = 0.002; OR = 0.26, 95% CI 0.20-0.69; p = 0.001, respectively). There are significant joint effects between smoking and CYP24A1 rs2181874, CYP24A1 rs6068816, VDR rs10735810, and VDR rs1544410 (p < 0.01-0.05). Smokers with mutated homozygous rs10735810 in VDR had significantly increased NSCLC risk (OR = 1.93, 95% CI 1.41-2.76; p = 0.015). In summary, the results suggested that the lower the distribution of vitamin D concentration, the more the genetic variations in CYP24A1, VDR and GC genes may be associated with NSCLC risk. In addition, there are significant joint associations of cigarette smoking and vitamin D deficiency on NSCLC risk. © 2016 by the authors; licensee MDPI, Basel, Switzerland.


PubMed | Kunming University, Yunnan Normal University and Hospital of Kunming Medical College
Type: Journal Article | Journal: International journal of molecular sciences | Year: 2016

Epidemiological studies regarding the relationship between vitamin D, genetic polymorphisms in the vitamin D metabolism, cigarette smoke and non-small cell lung cancer (NSCLC) risk have not been investigated comprehensively. To search for additional evidence, the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique and radioimmunoassay method were utilized to evaluate 5 single-nucleotide polymorphisms (SNPs) in vitamin D receptor (VDR), 6 SNPs in 24-hydroxylase (CYP24A1), 2 SNPs in 1-hydroxylase (CYP27B1) and 2 SNPs in vitamin D-binding protein (group-specific component, GC) and plasma vitamin D levels in 426 NSCLC cases and 445 controls from China. Exposure to cigarette smoke was ascertained through questionnaire information. Multivariable linear regressions and mixed effects models were used in statistical analysis. The results showed that Reference SNP rs6068816 in CYP24A1, rs1544410 and rs731236 in VDR and rs7041 in GC were statistically significant in relation to reduction in NSCLC risk (p < 0.001-0.05). No significant connection was seen between NSCLC risk and overall plasma 25-hydroxyvitamin D [25(OH)D] concentrations, regardless of smoking status. However, the mutation genotype of CYP24A1 rs6068816 and VDR rs1544410 were also significantly associated with increased 25(OH)D levels only in both the smoker and non-smoker cases (p < 0.01-0.05). Meanwhile, smokers and non-smokers with mutated homozygous rs2181874 in CYP24A1 had significantly increased NSCLC risk (odds ratio (OR) = 2.14, 95% confidence interval (CI) 1.47-3.43; p = 0.031; OR = 3.57, 95% CI 2.66-4.74; p = 0.019, respectively). Smokers with mutated homozygous rs10735810 in VDR had significantly increased NSCLC risk (OR = 1.93, 95% CI 1.41-2.76; p = 0.015). However, smokers with mutated homozygous rs6068816 in CYP24A1 had significantly decreased NSCLC risk (OR = 0.43, 95% CI 0.27-1.02; p = 0.006); and smokers and non-smokers with mutated homozygous rs1544410 in VDR had significantly decreased NSCLC risk (OR = 0.51, 95% CI 0.34-1.17; p = 0.002; OR = 0.26, 95% CI 0.20-0.69; p = 0.001, respectively). There are significant joint effects between smoking and CYP24A1 rs2181874, CYP24A1 rs6068816, VDR rs10735810, and VDR rs1544410 (p < 0.01-0.05). Smokers with mutated homozygous rs10735810 in VDR had significantly increased NSCLC risk (OR = 1.93, 95% CI 1.41-2.76; p = 0.015). In summary, the results suggested that the lower the distribution of vitamin D concentration, the more the genetic variations in CYP24A1, VDR and GC genes may be associated with NSCLC risk. In addition, there are significant joint associations of cigarette smoking and vitamin D deficiency on NSCLC risk.


Yang Z.,Tumor Hospital of Yunnan Province | Jin C.,Tumor Hospital of Yunnan Province | Chen T.,Tumor Hospital of Yunnan Province | Sun H.,Tumor Hospital of Yunnan Province | And 4 more authors.
Oncology Letters | Year: 2012

Brachytherapy is regarded as the most effective method in the treatment of metastatic spinal tumors since little damage is caused to surrounding healthy tissue. However, this method may cause radiation myelopathy if an overdose occurs. In the present study, we established a Banna mini-pig 125 I spinal cord implantation model to provide a tool for the study of how to reduce these types of side effects. Cell cycle alteration, apoptosis and necrosis of spinal cord neurons in the presence of various doses and durations of 125 I brachytherapy were also investigated. The pigs were randomly divided into four groups, A, B, C and D. In group A, four 125 I seeds (total radioactivity, 4.0 mCi) were implanted into the dura mater of the spinal canal at the level of T13. In groups B and C, eight 125 I sources (total radioactivity, 8.0 mCi) were inserted at the same location. Groups A and C were raised for up to 8 months and group B for only 2 months. Neurons from the swine spinal cord at the T13 level were collected and cell cycle analysis was performed. Apoptosis and necrosis were tested by a terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) assay. The Banna mini-pig brachytherapy model was successfully established. Radiation myelopathy was closely associated with radiation dose and duration, more neurons were blocked in the G2 and S phases as dose and time increased, and an increase in apoptosis and necrosis was detected. Ratios of apoptosis and necrosis were reduced as lower doses and shorter durations of radiation were applied. Our results demonstrate that the Banna mini-pig is an ideal animal to study 125 I brachytherapy. Low-dose and short-term brachytherapy may effectively decrease apoptosis and necrosis in spinal cord cells in Banna mini-pigs.


Niu J.-K.,Hospital of Kunming Medical College | Miao Y.-L.,Hospital of Kunming Medical College
World Chinese Journal of Digestology | Year: 2011

Ulcerative colitis (UC), one of non-specific chronic inflammatory conditions of the gastrointestinal tract with unknown complex etiology, is a chronic inflammatory bowel disorder characterized by diffuse mucosal inflammation of the colorectum with exacerbations and remissions. Nowadays, the diagnosis of UC is based mainly on symptoms, endoscopic findings, and histopathologic grading of biopsy specimens. However, there is a lack of a gold standard for the diagnosis of UC. Endoscopy is the cornerstone for diagnosis and evaluation of UC and plays a significant role in diagnosis, evaluating disease activity, malignancy surveillance and treatment. Over recent decades, the emergence of new imaging techniques, including endoscopic ultraonography, chromoendoscopy, magnification endoscopy, narrow-band endoscopic imaging, and laser scanning confocal microendoscopy, has provided a great boost to endoscopic diagnosis and treatment of UC. In this article, we will review the recent advances in endoscopic diagnosis and treatment of UC.

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