Liu X.-Z.,Hospital of Infectious Diseases of Yantai |
Zou Z.-Q.,Hospital of Infectious Diseases of Yantai |
Wang G.-Q.,Peking University |
Li D.,Shandong University |
Shao Z.-Y.,Hospital of Infectious Diseases of Yantai
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: Human umbilical cord mesenchymal stem cells (hUC-MSCs) can obviously relieve liver cirrhosis, and thereby repair liver injury. However, the molecular mechanism of hUC-MSCs therapy for liver cirrhosis is limited at present, and especially the non-coding RNA regulation of hepatic gene changes has not been detailed. OBJECTIVE: To investigate the changes of microRNA after hUC-MSCs therapy in rats with liver cirrhosis. METHODS: Liver cirrhosis models were established in rats using carbon tetrachloride subcutaneous injection plus oral administration of alcohol. At 8 weeks after modeling, hUC-MSCs were injected via the tail vein once a week for 4 consecutive weeks. At 1 week after the last injection, rat liver tissues were collected for paraffin embedding. Liver RNA was axtracted for gene chip analysis. Blood samplas were collacted and analyzed using an automatic biochemical analyzer to aetect the changes of liver function. RESULTS AND CONCLUSION: Alanine aminotransferase, aspartate aminotransferase and gamma-glutamyl transpeptidase were improved significantly after hUC-MSCs therapy. Fat lesions and necrosis of hepatocytes were significantly reduced. MicroRNA expression microarray hybridization analysis and PCR results showed that rno-miR-369-5p, rno-miR-3584-5p and rno-miR-153* were down-regulated during modeling and increased after hUC-MSCs therapy. And rno-miR-93, rno-miR-199a-3p, rno-miR-195, rno-let-7a and rno-miR-19a were firstly up-regulated in the process of modeling and then down-regulated obviously after hUC-MSCs therapy. These results suggest that hUC-MSCs may reverse liver cirrhosis and liver cell damage through up-regulation of rno-miR-369-5p, rno-miR-3584-5p and rno-miR-153*, and down-regulation of rno-miR-93, rno-miR-199a-3p, rno-miR-195, rno-let-7a and rno-miR-19a. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research, All Right Reserved.