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Ma S.,Hospital of Heilongjiang Province | Zhao Y.,Jiamusi University | Xia X.,Jiamusi University | Dong X.,Jiamusi University | And 2 more authors.
BioMed Research International | Year: 2015

Candida albicans (C.a) and Candida tropicalis (C.t) were treated with Streptococcus sanguinis bacteriocin (S.s bacteriocin), respectively; the bacteriostatic dynamics of S.s bacteriocin, their effects on cell surface hydrophobicity, leakage of inorganic phosphorus and macromolecular substance, cytosolic calcium concentration, and ultrastructure changes of Candida thallus were detected and analyzed. The results showed that inhibitory effect of S.s bacteriocin on C.a and C.t reached peak level at 24 h, the cell-surface hydrophobicity decreased significantly (P < 0.05) after S.s bacteriocin treatment, and there was leakage of cytoplasmic inorganic phosphorus and macromolecular substance from C.a and C.t; cytosolic calcium concentration decreased greatly. After 24 h treatment by S.s bacteriocin, depressive deformity and defect could be found in the cell surface of C.a and C.t; the thallus displayed irregular forms: C.a was shrunken, there was unclear margins abutting upon cell wall and cell membrane, nucleus disappeared, and cytoplasm was inhomogeneous; likewise, C.t was first plasmolysis, and then the cytoplasm was shrunk, the ultrastructure of cell wall and cell membrane was continuously damaged, and the nucleus was karyolysis. It was illustrated that S.s bacteriocin had similar antifungal effect on C.a and C.t; their cell surface hydrophobicity, membrane permeability, and ultrastructure were changed significantly on exposure to S.s bacteriocin. © 2015 Shengli Ma et al. Source


Liu Y.,Jilin University | Zhu M.,Harbin Institute of Technology | Lin L.,Hebei Medical University | Fan X.,Jilin University | And 2 more authors.
Brain Research | Year: 2014

Parkinsons disease (PD) is an age-related neurodegenerative movement disorder, characterized by loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc). The MPTP/MPP+ model is often used to investigate the signaling mechanisms of dopamine (DA) degeneration, both in vivo and in vitro. The identification of specific genetic and environmental factors responsible for PD has bolstered evidence for a shared pathway of neuronal death - apoptosis. Trim27 is reported to promote apoptosis. However, little evidence exists to indicate a linkage between Trim27 and PD. In this study, we found that compared to healthy individuals, Trim27 was significantly upregulated in patients with PD. We further showed that Trim27 expression was dramatically induced in PC12 cells and in the SNpc of the PD mouse model. RNAi-mediated knockdown of Trim27 in PC12 cells showed obvious suppression of apoptosis. There are reduced dopaminergic neuron loss and lower apoptotic protein expression levels in MPTP-treated Trim27-/- mice, compared with MPTP-treated WT mice. These data demonstrated that Trim27 deficiency decreases apoptosis and protects dopaminergic neurons in the neurotoxin model of PD, suggesting that Trim27 may be an effective potential target during the treatment of PD. © 2014 Elsevier B.V. Source


Li Q.,Childrens Hospital of Hebei Province | Song J.J.,Harbin Medical University | Zhang H.Y.,Harbin Medical University | Fu K.,Harbin Medical University | And 2 more authors.
Biotechnic and Histochemistry | Year: 2015

We investigated dexamethasone therapy for preventing delayed encephalopathy after carbon monoxide (CO) poisoning. Eighty healthy male rats were exposed to CO and randomly divided into four groups: hyperbaric oxygen treatment (H), treatment (D), combined hyperbaric and dexamethasone treatment (C), and a control (M) group in which the rats inhaled CO to coma in the hyperbaric oxygen chamber, then were removed without further treatment. Twelve rats were put into the hyperbaric oxygen chamber and treated with air for 60 min (N) group. An eight arm maze was used to evaluate cognitive and memory abilities of these mice. Serum myelin basic protein (MBP) levels were evaluated using ELISA, and magnetic resonance imaging was used to observe brain demyelination and morbidity associated with delayed encephalopathy. A sample of the hippocampus from each group was examined by light microscopy. Cognitive and memory functions decreased in the control group M. Three days after CO poisoning, the serum MBP level of each group increased significantly. On Day 10 after CO poisoning, the MBP levels in groups C and D decreased significantly, but returned to normal on Day 18. MBP levels in the M and H groups were elevated at all time points. Brain MRIs showed significant differences among C, D, H and control M groups. Hematoxylin & eosin staining of the hippocampus showed greater damage in the control M and H groups. Early dexamethasone treatment may be useful for preventing delayed encephalopathy after CO poisoning and may reduce serum MBP levels. © 2015 The Biological Stain Commission. Source


Fan X.,Jilin University | Jiang Y.,Jilin University | Jiang Y.,Jiangsu Electric Power Company | Han J.,Jilin University | And 4 more authors.
Mediators of Inflammation | Year: 2016

Objective. This study aimed to examine the potential role of memory T follicular helper (Tfh) cells in patients with neuromyelitis optica/neuromyelitis optica spectrum disorders (NMO/NMOSD). Methods. The percentages of different subsets of circulating memory Tfh cells in 25 NMO/NMOSD patients before and after treatment as well as in 17 healthy controls were examined by flow cytometry. The levels of IL-21 and AQP4 Ab in plasma and CSF were measured by ELISA. Results. The percentages and numbers of circulating memory Tfh cells, ICOS+, CCR7-, CCR7-ICOS+, CCR7+, CCR7+ICOS+ memory Tfh cells, and the levels of IL-21 in plasma and CSF were significantly increased in NMO/NMOSD patients. The percentages of CCR7- and CCR7-ICOS+ memory Tfh cells were positively correlated with ARR, plasma IL-21, and AQP4 Ab levels. The percentages of CCR7+ and CCR7+ICOS+ memory Tfh cells were positively correlated with CSF white blood cell counts, proteins, and IL-21 levels. Treatment with corticosteroids significantly reduced the numbers of CCR7-ICOS+ and CCR7+ICOS+ memory Tfh cells as well as plasma IL-21 levels in patients with partial remission. Conclusions. Our findings indicate that circulating memory Tfh cells may participate in the relapse and development of NMO/NMOSD and may serve as a new therapeutic target. © 2016 Xueli Fan et al. Source


Liu T.,Shenyang University | Ren X.,Shanghai JiaoTong University | Li L.,Guangdong General Hospital | Yin L.,Shenyang University | And 6 more authors.
Frontiers in Bioscience - Landmark | Year: 2015

Prostate cancer is the most common malignancy in men and is the second leading cause of cancer-related mortality in developed countries. Recent work has revealed the significance of CIP-interacting zinc finger protein 1 (CIZ1) in cancer cell biology, but its roles in prostatic carcinoma are unknown. Our study compared CIZ1 gene expression in banked prostatic carcinomas versus matched paraneoplastic tissues and in tumor cell lines of varying origin. This study revealed that the expression of CIZ1 was higher in high-grade prostate cancer than in low-grade prostate cancer and normal tissues. Among the tumor cell lines, PC-3 exhibited the highest levels of CIZ1 expression. CIZ1 gene silencing in PC-3 cells reduced cell proliferation and colony formation, induced cell cycle arrest in G1, inhibited tumor formation in nude mice, and suppressed the expression of genes related to prostate carcinoma. These results suggest that CIZ1 may play an important role in the progression of human prostate carcinoma and us which may be used as a therapeutic target in prostate cancer. © 2014, Frontiers in Bioscience. All rights reserved. Source

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